Micropropagation of Kaempferia angustifolia Roscoe via Direct Regeneration

作     者：Zuraida Ab Rahman Ayu Nazreena Othman Mohd Norfaizal Ghazalli Nur Auni Syazalien Adlan Zuraida Ab Rahman;Ayu Nazreena Othman;Mohd Norfaizal Ghazalli;Nur Auni Syazalien Adlan

作者机构：Biotechnology & Nanotechnology Research Centre MARDI HQ Persiaran MARDI-UPM Serdang Selangor Malaysia Agrobiodiversity & Environment Research Center MARDI HQ Persiaran MARDI-UPM Serdang Selangor Malaysia Gambang Campus Universiti Malaysia Pahang Pahang Malaysia 

出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)

年 卷 期：2022年第13卷第6期

页      面：734-743页

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

主　　题：BAP NAA Micropropagation Kaempferia angustifolia Direct Regeneration 

摘      要：This study has established an efficient and reproducible protocol to micropropagation Kaempferia angustifolia Roscoe via direct regeneration. The use of young shoots as explants showed the best results compared to rhizome shoots, where the young shoots showed a low percentage of contamination of 10% - 30% (agar 6 g/L) and 45% - 55% (agar 3 g/L), respectively, compared to the use of rhizome shoots, where the contamination rate exceeded 80%. For shoot initiation, the combination of BAP (6 Benzylaminopurine) and NAA (1-Naphthaleneacetic acid) showed higher results for the percentage of initial shoots and number of micro shoots/explants compared to BAP with Kin (Kinetin). The highest concentration of BAP (5 mg/L) combined with the lowest concentration of NAA (0.5 mg/L) resulted in 90% of initial shoots and a number of shoots/explants of 5.8. The highest number of shoots for micropropagation was in treatment with 30 g/L sucrose that was segmented with 3 mg/L BAP + 0.5 mg/L NAA. For the number of roots, the highest number of roots was 11.8 recorded at sucrose (45) with only BAP (1 mg/L) used as the plant growth regulator, while the longest length of roots was 7 - 8 cm, recorded both at sucrose with the combination of BAP and NAA.