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Proposal for a new evaluation of phagocytosis using different sizes of fluorescent polystyrene microspheres

Advances in Biological Chemistry 2013年第6期3卷 556-563页

作者： Riyo Enomoto Makoto Imamori Ayoumi Seon Kozue Yoshida aya furue Hirofumi Tsuruda Eibai Lee-Hiraiwa Laboratory of Pharmacology Faculty of Pharmaceutical Sciences Kobe Gakuin University Kobe Japan Laboratory of Pharmacology Faculty of Pharmaceutical Sciences Kobe Gakuin University Kobe Japan 2Cooperative Research Center of Life Sciences Kobe Gakuin University Kobe Japan Laboratory of Pharmacology Faculty of Pharmaceutical Sciences Kobe Gakuin University Kobe Japan 2Cooperative Research Center of Life Sciences Kobe Gakuin University Kobe Japan Cooperative Research Center of Life Sciences Kobe Gakuin University Kobe Japan Laboratory of Pharmacology Faculty of Pharmaceutical Sciences Kobe Gakuin University Kobe Japan Cooperative Research Center of Life Sciences Kobe Gakuin University Kobe Japan 2Cooperative Research Center of Life Sciences Kobe Gakuin University Kobe Japan

To investigate phagocytosis, peritoneal-resident and J774.1 macrophages were incubated with fluorescent polystyrene microspheres measuring 1.0 μm in diamter at 200 particles per cell. The amount of phagocytized microspheres increased with incubation time, and both cell types had similar phagocytic activity. Further, we investigated the phagocytosis of different sizes of microspheres by J774.1 macrophages. To adequately evaluate phagocytosis, varying amounts of different sizes of microspheres were added to J774.1 cells, and their phagocytic activities were evaluated. When the microspheres were added at a density of 20 particles per cell, few small microspheres (3 μm in diameter) could not be evaluated because of cytotoxicity. Thus, the amount of different sizes of microspheres added is important for precisely evaluating phagocytic activity. When the amount of different sizes of microspheres added was standardized to provide a set amount of total surface area, phagocytosis of these microspheres could be adequately evaluated and compared. To determine the effects of phagocytosis on cell viability and proliferation, cells incubated with different sizes of microspheres were assayed using a cell counting kit. We found that phagocytosis had no effect on cell viability or proliferation and was independent of particle size. Furthermore, cells already phagocytized microspheres retained their phagocytic activity.

关键词： Macrophage Phagocytosis Polystyrene Microsphere Total Surface Area

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Six Amino Acids among Natural Moisturizing Factors Responsible for Skin Hydration: Improvement and Anti-Aging of Skin by Galactomyces Ferment Filtrate-PiteraTM Containing Skin Moisturizer

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Journal of Cosmetics, Dermatological Sciences and Applications 2024年第2期14卷 113-127页

作者： Kukizo Miyamoto Yoko Munakata Keisuke Fujii Wang Summer Ley Yang Suda Sudarsana Masutaka furue Research and Development Kobe Innovation Center Procter and Gamble Innovation GK Kobe Japan Global Scientific Communications P & G Guangzhou Ltd. Guangzhou China Department of Dermatology Graduate School of Medical Sciences Kyushu University Fukuoka Japan

Background: Natural moisturizing factors (NMFs) are filaggrin-derived components in the cornified layer that are critical for maintaining healthy skin moisturization and barrier function. However, studies have reported conflicting findings on the relationship between NMF levels and aging, while few studies have investigated this relationship clinically. To fill this research gap, we determined the levels of major NMF components such as free amino acids, pyrrolidone carboxylic acid, and urocanic acids, and individually verified their relationships with skin hydration, barrier function, age, and skin aging. Purpose: The objective of this study was to clinically investigate the relationship between NMF components levels and skin aging in facial skin. The main NMF components were obtained from facial skin and quantified. We then selected NMF components showing strong relationships to skin hydration, and analyzed the relationships of the levels of these selected NMF components with signs of skin aging, namely, texture, pores, wrinkles, and dullness (L-value). We also examined the efficacy of treatment with a skin care formula (SK-II Facial Treatment Essence, called SK-II FTE hereafter) including Galactomyces ferment filtrate (GFF, PiteraTM) on the selected NMF component levels associated with skin hydration and barrier function, and the signs of skin aging of texture, pores, wrinkles, and dullness (L-value). Method: We conducted two clinical trials in this research. In Study 1, we measured 23 NMF components using tape-stripped cornified layer to quantify them via an HPLC method in 196 Asian females aged 20 to 59 (mean S.D., 38.6 9.4). Facial visual aging parameters [texture, pores, wrinkles, and dullness (L-value)], as well as elasticity (R7), skin hydration, and TEWL, were quantified using facial skin imaging and skin physical property measurement devices. Study 2 was performed to evaluate whether the facial application of SK-II FTE affects the NMF levels and skin aging

关键词： NMF Amino Acid Visual Aging Parameter Hydration Aging Texture Pore Wrinkle Dullness Galactomyces Ferment Filtrate PiteraTM SK-II Facial Treatment Essence

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Quantification of In Vivo Epidermal Keratinocyte Architecture Associated with the Signs of Skin Aging and the Skin Benefit Evaluation by Application of Galactomyces Ferment Filtrate (Pitera)-Containing Skin Care Product

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Journal of Cosmetics, Dermatological Sciences and Applications 2024年第1期14卷 12-28页

作者： Kukizo Miyamoto Yoko Munakata Keisuke Fujii Chenlu Lei Ley Yang Suda Sudarsana Masutaka furue Research and Development Kobe Innovation Center Procter and Gamble Innovation GK Kobe Japan Global Scientific Communications P & G Guangzhou Ltd. Guangzhou China Department of Dermatology Graduate School of Medical Sciences Kyushu University Fukuoka Japan

Background: Aged skin exhibits visual alterations such as wrinkles, rough texture, pore dilation, and dull skin tone, as well as physiological aging, namely, decreased hydration and increased transepidermal water loss (TEWL). Recent advances in coherence tomography have also revealed that skin aging affects in vivo epidermal keratinocyte architecture. However, the interconnectivity between spatial architectural aging and visual/physiological aging parameters remains largely unknown. Purpose: To elucidate whether the tomographic keratinocyte architectural aging is correlated with visual and physiological skin aging parameters and to quantitatively evaluate the improvements of the architectural, visual, and physiological aging parameters by the daily treatment of the skin care formula containing Galactomyces Ferment Filtrate (GFF, 8X PiteraTM). Method: We measured the in vivo keratinocyte cellular architecture with two-photon stereoscopic tomography obtaining by-layer epidermal section images in 78 Asian females of various ages. Visual aging parameters were analyzed using a portable image capture system. Hydration and TEWL were also assessed. The anti-aging effects of GFF-containing skin moisturizer (SK-II LXP CreamTM) were also examined in two studies after twice-daily application for 2 (N = 35) and 4 (N = 32) weeks. Results: As for the keratinocyte cellular architecture, skin aging was significantly associated with decreased cell density and increased cell uniformity. These architectural aging parameters were significantly correlated with visual and physiological aging parameters, namely, rough texture, wrinkles, pore dilation, dull skin tone, dehydration, and increased TEWL. The strong interconnectivity allowed us to develop formulae to estimate the keratinocyte architecture from visual aging parameters. Moreover, twice-daily application of SK-II significantly improved the keratinocyte architecture associated with multiple skin aging visual an

关键词： Facial Skin Aging In Vivo Keratinocyte Cellular Architecture Visual Aging Pa-rameter Dehydration Interconnectivity New Trajectory Galactomyces Fer-ment Filtrate PiteraTM SK-II LXP CreamTM

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Expanded Follicle-Sulcus-Crack Complex Is an Early Warning Sign of Facial Skin Aging: Improvement by Application of Galactomyces Ferment Filtrate-Containing Skin Product

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Journal of Cosmetics, Dermatological Sciences and Applications 2023年第2期13卷 91-106页

作者： Kukizo Miyamoto Bandara Dissanayake Shenal Wanigasekara Keisuke Fujii Xianghong Yan Masutaka furue Research and Development Kobe Innovation Center Procter and Gamble Innovation GK Kobe Japan Department of Research and Development Beauty Technology Division P&G Company Cincinnati USA Department of Dermatology Graduate School of Medical Sciences Kyushu University Fukuoka Japan

Background: Wrinkles, pigmented spots, and roughness are representative parameters reflecting facial skin aging. Sulci cutis connecting to follicular orifices frequently form perifollicular cracks, which join together adjacent sulci. This follicle-sulcus-crack complex (FSCC) is exacerbated by dehydration. Purpose: Although dehydration is associated with facial skin aging, FSCC’s implications in facial skin aging remain unresolved. Method: We measured facial skin parameters in 1999 and 2010 in 108 Japanese women, and in 2006 and 2016 in 182 Chinese women. We addressed whether expansion of FSCC is associated with other skin aging parameters. We also examined whether skin moisturizer can reverse the expanded FSCC. Results: In both clinical studies, FSCC severity as well as wrinkles, spots, and roughness significantly deteriorated with age. Notably, FSCC significantly increased as early as in subjects in their 20s, whereas wrinkles, spots, and roughness became conspicuous in those in their 40s or older. Moreover, subjects with more severe FSCC in the initial measurement exhibited significantly worse wrinkles, spots, and roughness in the second measurement a decade later. Dehydration was significantly correlated with expanded FSCC. Furthermore, FSCC expansion was reversed after appropriate moisturization by daily application of Galactomyces ferment filtrate (GFF)-containing skincare product (SK-II Skin Power Cream) for 4 weeks in 37 women. Conclusion: The expanded FSCC is likely to be an early indicator of facial skin aging. Appropriate moisturization may reduce FSCC formation and decelerate facial skin aging.

关键词： Facial Skin Aging Follicle-Sulcus-Crack Complex Dehydration Hyperpigmented Spot Wrinkle Galactomyces Ferment Filtrate

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INHIBITORY EFFECT OF NOCICEPTIN ON EXCITATORY BUT NOT INHIBITORY TRANSMISSION IN RAT SPINAL DORSAL HORN NEURONES

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神经解剖学杂志 2001年第B7期17卷 110-110页

作者： Eiichi KUMAMOTO Hidemasa furue Megumu YOSHIMURA Department of Physiology Saga Medical School Saga 849-8501 Japan

Nociceptin, or orphanin FQ, is a 17 amino-acid peptide which is a newly discovered endogenous ligand for the opioid-like G-protein coupled receptor, ORLI, and suggested to participate in modulating nociceptive transmission. We examined the actions of nociceptin on excitatory and inhibitory transmission in substantia gelatinosa (SG) neurons in the dorsal horn of adult rat spinal cord slices by use of the blind whole-cell patch-clamp technique. Nociceptin induced an outward current at-70 mV in a dose-dependent manner (EC_(50)=0.23μM);

关键词：大白鼠脊髓背角神经配体损伤感受素兴奋传导抑制作用 SG

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PRESYNAPTIC Al ADENOSINE RECEPTOR-MEDIATED INHIBITION OF NOCICEPTIVE TRANSMISSION TO SPINAL DORSAL HORN NEURONS IN THE ADULT RAT

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神经解剖学杂志 2001年第B7期17卷 116-116页

作者： Eiichi KUMAMOTO Hidemasa furue Megumu YOSHIMURA Department of Physiology Saga Medical School 5-1-1 Nabeshima Saga 849-8501 Japan

Although intrathecal administration of adenosine analogues or A_1 adenosine receptor agonists is known to result in antinociception, this has not been examined yet at the cellular level. In the present study, we examined pharmacologically the actions of adenosine on spontaneous sensory transmission in substantia gelatinosa (SG) neurons of an adult rat spinal cord slice under the condition where a postsynaptic action of adenosine

关键词：成年大白鼠脊髓背角神经损伤感受传导突触前抑制 A1腺苷受体调制性抑制疼痛

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Expanded Follicle-Sulcus-Crack Complex Is an Early Warning Sign of Facial Skin Aging:Improvement by Application of Galactomyces Ferment Filtrate-Containing Skin Product

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Journal of Cosmetics, Dermatological Sciences and Applications 2023年第2期

作者： Kukizo Miyamoto Bandara Dissanayake Shenal Wanigasekara Keisuke Fujii Xianghong Yan Masutaka furue

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前列腺特异性抗原和雄激素受体在乳房外paget病中的表达

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临床皮肤科杂志 2007年第2期36卷 77-79页

作者：林能兴刘厚君占部和敬古江增隆涂亚庭华中科技大学同济医学院附属协和医院皮肤科湖北武汉430022 日本九州大学附属病院皮肤科

目的:了解前列腺特异性抗原(prostate specific antigen,PSA)和雄激素受体(androgen receptor,AR)在乳房外Paget病(EMPD)中的表达及其意义。方法:采用免疫组化ABC法检测PSA和AR在30例EMPD患者皮损中的表达情况。结果:①在30例EMPD患者... 详细信息

目的:了解前列腺特异性抗原(prostate specific antigen,PSA)和雄激素受体(androgen receptor,AR)在乳房外Paget病(EMPD)中的表达及其意义。方法:采用免疫组化ABC法检测PSA和AR在30例EMPD患者皮损中的表达情况。结果:①在30例EMPD患者皮损中13例PSA表达阳性,14例AR表达阳性,且在男性患者中PSA和AR的表达均明显高于女性患者(P<0.01);②在男性EMPD患者中,PSA和AR阳性表达间有明显相关性。结论:PSA和AR在男性EMPD患者中过度表达,PSA和(或)AR信号传导通路可能在男性EMPD形成机制中起重要作用。

关键词： Paget病乳房外抗原前列腺特异性受体雄激素

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磷酸化Stat3和磷酸化Akt及细胞周期蛋白D1在血管肉瘤中的表达

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中华皮肤科杂志 2007年第3期40卷 155-157页

作者：林能兴连昕占部和敬古江增隆涂亚庭华中科技大学同济医学院附属协和医院皮肤科武汉430022 日本九州大学附属病院皮肤科

目的探讨磷酸化细胞信号传导与转录活化因子3（Star3）和磷酸化Akt及细胞周期蛋白D1在血管肉瘤中的表达。方法采用免疫组化ABC的方法检测磷酸化Star3（p-Stat3）和磷酸化Akt（p-Akt）及细胞周期蛋白D1在21例血管肉瘤和15例毛细血管瘤... 详细信息

目的探讨磷酸化细胞信号传导与转录活化因子3（Star3）和磷酸化Akt及细胞周期蛋白D1在血管肉瘤中的表达。方法采用免疫组化ABC的方法检测磷酸化Star3（p-Stat3）和磷酸化Akt（p-Akt）及细胞周期蛋白D1在21例血管肉瘤和15例毛细血管瘤石蜡包埋切片中的表达。结果在21例血管肉瘤石蜡包埋切片中，有15例p-Stat3表达阳性，11例p-Akt表达阳性，16例细胞周期蛋白D1表达阳性；而在15例毛细血管瘤中仅有4例p-Stat3表达阳性，2例p-Akt表达阳性，4例细胞周期蛋白D1表达阳性，两组各阳性表达率分别经卡方检验发现，差异均有统计学意义（P〈0．05）。在血管肉瘤中，p-Star3阳性表达与细胞周期蛋白D1的阳性表达有相关性（r=0．62，P=0．003），而p-Akt阳性表达与细胞周期蛋白D1的阳性表达无相关性（r=-0．09，P=0．700）。结论 p-Stat3、p-Akt和细胞周期蛋白D1在血管肉瘤的形成机制中可能起一定的作用。

关键词：血管肉瘤血管瘤毛细管细胞周期蛋白D1 蛋白激酶类

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毛母质瘤中β联蛋白的突变基因分析

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中华皮肤科杂志 2006年第9期39卷 497-499页

作者：夏建新占部和敬李玉林古江增隆吉林大学白求恩医学部第二医院皮肤科长春130041 日本九州大学医学部皮肤科吉林大学基础医学院病理生物学教研室

目的探讨β联蛋白的突变基因及其蛋白表达与毛母质瘤发生的关系。方法选取67例毛母质瘤的组织标本进行β联蛋白的免疫染色,提取基因组DNA,进行β联蛋白的PCR扩增及测序,用克隆、测序及限制性内切酶的方法证实突变的存在。结果毛母质瘤... 详细信息

目的探讨β联蛋白的突变基因及其蛋白表达与毛母质瘤发生的关系。方法选取67例毛母质瘤的组织标本进行β联蛋白的免疫染色,提取基因组DNA,进行β联蛋白的PCR扩增及测序,用克隆、测序及限制性内切酶的方法证实突变的存在。结果毛母质瘤中嗜碱粒细胞β联蛋白免疫染色阳性率为81%(26/32),β联蛋白外显子3基因突变率为61%(14/23)。其突变均位于β联蛋白外显子3的磷酸化位点或邻近位点。结论β联蛋白的基因突变及其核阳性与毛母质瘤的发生有关。

关键词：毛基质瘤突变 β联蛋白

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