检索结果-南通市图书馆

Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus

Asian Pacific Journal of Tropical Biomedicine 2017年第7期7卷 633-639页

作者： Achmad Fuad Hafid Chie Aoki-Utsubo Adita Ayu Permanasari Myrna Adianti Lydia Tumewu aty widyawaruyanti Sri Puji Astuti Wahyuningsih Tutik Sri Wahyuni Maria Inge Lusida Soetjipto Hak Hotta Institute of Tropical Disease Airlangga University Surabaya 60115 IndonesiaDepartment of Pharmacognosy and Phytochemistry Faculty of Pharmacy Airlangga University Surabaya 60286 Indonesia Department of International Health Kobe University Graduate School of Health Sciences 7-10-2 Tomogaoka Suma-ku Kobe 654-0142 Japan Institute of Tropical Disease Airlangga University Surabaya 60115 Indonesia Department of Biology Faculty of Science and Technology Airlangga University Surabaya 60115 Indonesia Institute of Tropical Disease Airlangga University Surabaya 60115 IndonesiaDepartment of Microbiology Faculty of Medicine Airlangga University Surabaya 60131 Indonesia Institute of Tropical Disease Airlangga University Surabaya 60115 IndonesiaDepartment of Biochemistry Faculty of Medicine Airlangga University Surabaya 60131 Indonesia Department of Oral Vaccine and Drug Development Kobe University Graduate School of Health Sciences 1-5-6 Minatojima-minamimachi Chou-ku Kobe 650-0047 Japan

Objective: To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods: Antiviral activities of the crude extracts were examined by cell culture method using Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCV activities was determined by time-of-addition experiments. The effect on HCV RNA replication and HCV accumulation in cells were analyzed by quantitative reverse transcription-PCR and western blotting, respectively. Results: The dichloromethane (DCM) extract of A. heterophyllus exhibited strong anti-HCV activity with an inhibitory concentration (IC50) value of (1.5 ± 0.6)μg/mL without obvious toxicity. The DCM extracts from Artocarpus altilis and Artocarpus camansi showed moderate anti-HCV activities with IC50 values being (6.5 ± 0.3) μg/mL and (9.7 ± 1.1) μg/mL, respectively. A time-of-addition studies showed that DCM extract from A. heterophyllus inhibited viral entry process though a direct virucidal activity and targeting host cells. HCV RNA replication and HCV protein expression were slightly reduced by the DCM treatment at high concentration. Conclusions: The DCM extract from A. heterophyllus is a good candidate to develop an antiviral agent to prevent HCV grant reinfection following liver transplantation.

关键词： Hepatitis C virus Artocarpus sp. Artocarpus heterophyllus Antiviral

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：