Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus

作     者：Achmad Fuad Hafid Chie Aoki-Utsubo Adita Ayu Permanasari Myrna Adianti Lydia Tumewu Aty Widyawaruyanti Sri Puji Astuti Wahyuningsih Tutik Sri Wahyuni Maria Inge Lusida Soetjipto Hak Hotta 

作者机构：Institute of Tropical Disease Airlangga University Surabaya 60115 IndonesiaDepartment of Pharmacognosy and Phytochemistry Faculty of Pharmacy Airlangga University Surabaya 60286 Indonesia Department of International Health Kobe University Graduate School of Health Sciences 7-10-2 Tomogaoka Suma-ku Kobe 654-0142 Japan Institute of Tropical Disease Airlangga University Surabaya 60115 Indonesia Department of Biology Faculty of Science and Technology Airlangga University Surabaya 60115 Indonesia Institute of Tropical Disease Airlangga University Surabaya 60115 IndonesiaDepartment of Microbiology Faculty of Medicine Airlangga University Surabaya 60131 Indonesia Institute of Tropical Disease Airlangga University Surabaya 60115 IndonesiaDepartment of Biochemistry Faculty of Medicine Airlangga University Surabaya 60131 Indonesia Department of Oral Vaccine and Drug Development Kobe University Graduate School of Health Sciences 1-5-6 Minatojima-minamimachi Chou-ku Kobe 650-0047 Japan 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2017年第7卷第7期

页      面：633-639页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported in part by Mandat Project Airlangga University and Science and Technology Research Partnerships for Sustainable Development (SATREPS) program from Japan Science and Technology Agency (JST) and Japan International Cooperation Agency (JICA) 

主　　题：Hepatitis C virus Artocarpus sp. Artocarpus heterophyllus Antiviral 

摘      要：Objective: To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods: Antiviral activities of the crude extracts were examined by cell culture method using Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCV activities was determined by time-of-addition experiments. The effect on HCV RNA replication and HCV accumulation in cells were analyzed by quantitative reverse transcription-PCR and western blotting, respectively. Results: The dichloromethane (DCM) extract of A. heterophyllus exhibited strong anti-HCV activity with an inhibitory concentration (IC50) value of (1.5 ± 0.6)μg/mL without obvious toxicity. The DCM extracts from Artocarpus altilis and Artocarpus camansi showed moderate anti-HCV activities with IC50 values being (6.5 ± 0.3) μg/mL and (9.7 ± 1.1) μg/mL, respectively. A time-of-addition studies showed that DCM extract from A. heterophyllus inhibited viral entry process though a direct virucidal activity and targeting host cells. HCV RNA replication and HCV protein expression were slightly reduced by the DCM treatment at high concentration. Conclusions: The DCM extract from A. heterophyllus is a good candidate to develop an antiviral agent to prevent HCV grant reinfection following liver transplantation.