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Hypoxia-inducible factor 1alpha and vascular endothelial growth factor in Glioblastoma Multiforme:a systematic review going beyond pathologic implications

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Oncology Research 2024年第8期32卷 1239-1256页

作者： DIMITRA P.VAGELI PANAGIOTIS G.DOUKAS KERASIA GOUPOU ANTONIOS D.BENOS kyriaki astara KONSTANTINA ZACHAROULI SOTIRIS SOTIRIOU MARIA IOANNOU Department of Surgery Yale UniversityNew HavenCT 06510USA Department of Pathology Faculty of MedicineSchool of Health SciencesUniversity of ThessalyLarissa41500Greece Department of Medicine Rutgers/Saint Peter’s University HospitalNew BrunswickNJ 08901USA Department of Neurology Army Share Fund Hospital(NIMTS)Athens11521Greece Laboratory of Embryology Faculty of MedicineSchool of Health SciencesUniversity of ThessalyLarissa41500Greece

Glioblastoma multiforme(GBM)is an aggressive primary brain tumor characterized by extensive heterogeneity and vascular *** conditions in the tissue microenvironment are considered a pivotal player leading tumor ***,hypoxia is known to activate inducible factors,such as hypoxia-inducible factor 1alpha(HIF-1α),which in turn can stimulate tumor neo-angiogenesis through activation of various downward mediators,such as the vascular endothelial growth factor(VEGF).Here,we aimed to explore the role of HIF-1α/VEGF immunophenotypes alone and in combination with other prognostic markers or clinical and image analysis data,as potential biomarkers of GBM prognosis and treatment *** performed a systematic review(Medline/Embase,and Pubmed database search was completed by 16th of April 2024 by two independent teams;PRISMA 2020).We evaluated methods of immunoassays,cell viability,or animal or patient survival methods of the retrieved studies to assess unbiased *** used inclusion criteria,such as the evaluation of GBM prognosis based on HIF-1α/VEGF expression,other biomarkers or clinical and imaging manifestations in GBM related to HIF-1α/VEGF expression,application of immunoassays for protein expression,and evaluation of the effectiveness of GBM therapeutic strategies based on HIF-1α/VEGF *** used exclusion criteria,such as data not reporting both HIF-1αand VEGF or *** included 50 studies investigating in total 1319 GBM human specimens,18 different cell lines or GBM-derived stem cells,and 6 different animal models,to identify the association of HIF-1α/VEGF immunophenotypes,and with other prognostic factors,clinical and macroscopic data in GBM prognosis and therapeutic *** found that increased HIF-1α/VEGF expression in GBM correlates with oncogenic factors,such as miR-210-3p,Oct4,AKT,COX-2,PDGF-C,PLDO3,M2 polarization,or ALK,leading to unfavorable *** HIF-1α/VEGF expression correlates with F

关键词： Glioblastoma multiforme(GBM) Astrocytoma Grade III Astrocytoma Grade IV Hypoxia-inducible factor 1alpha(HIF-1α) Vascular endothelial growth factor(VEGF)

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Athletes with mild post-COVID-19 symptoms experience increased respiratory and metabolic demands:А cross-sectional study

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Sports Medicine and Health Science 2023年第2期5卷 106-111页

作者： Vasileios T.Stavrou astara kyriaki George D.Vavougios Ioannis G.Fatouros George S.Metsios Konstantinos Kalabakas Dimitrios Karagiannis Zoe Daniil Konstantinos I.Gourgoulianis George Вasdekis Laboratory of Cardio-Pulmonary Testing and Pulmonary Rehabilitation Respiratory Medicine DepartmentFaculty of MedicineUniversity of ThessalyLarissaGreece The Medical Project PreventionEvaluation and Recovery CenterLarissaGreece Department of Neurology 417 Army Equity Fund Hospital(NIMTS)AthensGreece Department of Neurology Faculty of MedicineUniversity of CyprusLefkosiaCyprus School of Physical Education and Sport Sciences University of ThessalyTrikalaGreece Department of Nutrition and Dietetics University of ThessalyTrikalaGreece

Coronavirus Disease 2019(COVID-19)has significantly affected different physiological systems,with a potentially profound effect on athletic ***,to date,such an effect has been neither addressed nor ***,the aim of this study was to investigate fitness indicators,along with the respiratory and metabolic profile,in post-COVID-19 *** male soccer players,were divided into two groups:non-hospitalized COVID-19(n=20,Age:[25.2±4.1]years,Body Surface Area[BSA]:[1.9±0.2]m^(2),body fat:11.8%±3.4%)versus[vs]healthy(n=20,Age:[25.1±4.4]years,BSA:[2.0±0.3]m^(2),body fat:10.8%±4.5%).For each athlete,prior to cardiopulmonary exercise testing(CPET),body composition,spirometry,and lactate blood levels,were *** between groups were assessed with the independent samples t-test(p<0.05).Several differences were detected between the two groups:ventilation(:Resting:[14.7±3.1]L·min^(−1)vs.[11.5±2.6]L·min^(−1),p=0.001;Maximal Effort:[137.1±15.5]L·min^(−1)vs.[109.1±18.4]L·min^(−1),p<0.001),ratio VE/maximal voluntary ventilation(Resting:7.9%±1.8%vs.5.7%±1.7%,p<0.001;Maximal Effort:73.7%±10.8%vs.63.1%±9.0%,p=0.002),ratioVE/BSA(Resting:7.9%±2.0%vs.5.9%±1.4%,p=0.001;Maximal Effort:73.7%±11.1%vs.66.2%±9.2%,p=0.026),heart rate(Maximal Effort:[191.6±7.8]bpm vs.[196.6±8.6]bpm,p=0.041),and lactate acid(Resting:[1.8±0.8]mmol·L^(−1)vs.[0.9±0.1]mmol·L^(−1),p<0.001;Maximal Effort:[11.0±1.6]mmol·L^(−1)vs.[9.8±1.2]mmol·L^(−1),p=0.009),during *** significant differences were identified regarding maximal oxygen uptake([55.7±4.4]ml·min^(−1)·kg−1 vs.[55.4±4.6]ml·min^(−1)·kg−1,p=0.831).Our findings demonstrate a pattern of compromised respiratory function in post-COVID-19 athletes characterized by increased respiratory work at both rest and maximum effort as well as hyperventilation during exercise,which may explain the reported increased metabolic needs.

关键词： Cardiopulmonary exercise testing Infected with COVID-19 Respiratory work

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Impact of hepatitis B immunoglobulin mode of administration on treatment experiences of patients after liver transplantation: Results from an online survey

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World Journal of Transplantation 2024年第3期14卷 144-155页

作者： Giorgia Rizza kyriaki Glynou Masha Eletskaya General Surgery and Liver Transplant Center S.Giovanni Battista HospitalTurin I-10126Italy Medical Affairs Biotest AGDreieich 63303Germany Lumanity Insight(Cello Health Insight) London SE11PPUnited Kingdom

BACKGROUND Hepatitis B immunoglobulin(HBIG)in combination with a potent nucleos(t)ide analog is considered the standard of care for prophylaxis against hepatitis B virus(HBV)reinfection after liver transplantation for HBV-associated *** To evaluate patients’satisfaction,preferences,and requirements for subcutaneous(SC),intramuscular(IM),and intravenous(IV)HBIG *** A self-completion,cross-sectional,online,22-question survey was conducted to examine perceptions and satisfaction with current HBIG treatment in adults receiving HBIG treatment following liver transplantation for HBV-associated disease in France,Italy,and *** HBIG products with different administration modes were evaluated using target product profile assessment and a conjoint(trade-off)*** Ninety patients were enrolled;32%,17%,and 51%were SC,IM,and IV HBIG users,*** duration of treatment was 36.2 *** HBIG had the least negative impact on emotional well-being and social life and was perceived as the most convenient,easiest to administer,least painful,and had the highest self-rating of treatment *** IM HBIG users than SC or IV HBIG users reported that administration frequency was excessive(67%,28%,and 28%,respectively).In the target product profile assessment,76%of patients were likely to use hypothetical SC *** the conjoint exercise,administration route,frequency,and duration were key drivers of treatment *** Ease,frequency,duration,and side effects of HBIG treatment administration were key drivers of treatment preferences,and SC HBIG appeared advantageous over IM and IV HBIG for administration ease,convenience,and pain.A hypothetical SC HBIG product elicited a favorable *** demographics,personal preferences,and satisfaction with HBIG treatment modalities may influence long-term treatment compliance.

关键词： Hepatitis B immunoglobulin Liver transplantation Subcutaneous Intramuscular Intravenous Patient satisfaction

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Incidence, risk factors and clinical outcome of multidrug-resistant organisms after heart transplantation

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World Journal of Transplantation 2024年第2期14卷 107-118页

作者： Sophia Hatzianastasiou Paraskevas Vlachos Georgios Stravopodis Dimitrios Elaiopoulos Afentra Koukousli Josef Papaparaskevas Themistoklis Chamogeorgakis Kyrillos Papadopoulos Theodora Soulele Despoina Chilidou kyriaki Kolovou Aggeliki Gkouziouta Michail Bonios Stamatios Adamopoulos Stavros Dimopoulos Microbiology Department and Infection Control Office Onassis Cardiac Surgery CenterAthens 17674Greece Heart Transplantation Unit Onassis Cardiac Surgery CenterAthens 17674Greece Cardiac Surgery Intensive Care Unit Onassis Cardiac Surgery CenterAthens 17674Greece

BACKGROUND Transplant recipients commonly harbor multidrug-resistant organisms(MDROs),as a result of frequent hospital admissions and increased exposure to antimi-crobials and invasive *** To investigate the impact of patient demographic and clinical characteristics on MDRO acquisition,as well as the impact of MDRO acquisition on intensive care unit(ICU)and hospital length of stay,and on ICU mortality and 1-year mortality post heart *** This retrospective cohort study analyzed 98 consecutive heart transplant patients over a ten-year period(2013-2022)in a single transplantation *** was collected regarding MDROs commonly encountered in critical *** Among the 98 transplanted patients(70%male),about a third(32%)acquired or already harbored MDROs upon transplantation(MDRO group),while two thirds did not(MDRO-free group).The prevalent MDROs were Acinetobacter baumannii(14%),Pseudomonas aeruginosa(12%)and Klebsiella pneumoniae(11%).Compared to MDRO-free patients,the MDRO group was characterized by higher body mass index(P=0.002),higher rates of renal failure(P=0.017),primary graft dysfunction(10%vs 4.5%,P=0.001),surgical re-exploration(34%vs 14%,P=0.017),mechanical circulatory support(47%vs 26%P=0.037)and renal replacement therapy(28%vs 9%,P=0.014),as well as longer extracorporeal circulation time(median 210 vs 161 min,P=0.003).The median length of stay was longer in the MDRO group,namely ICU stay was 16 vs 9 d in the MDRO-free group(P=0.001),and hospital stay was 38 vs 28 d(P=0.006),while 1-year mortality was higher(28%vs 7.6%,log-rank-χ2:7.34).CONCLUSION Following heart transplantation,a predominance of Gram-negative MDROs was *** acquisition was associated with higher complication rates,prolonged ICU and total hospital stay,and higher post-transplantation mortality.

关键词： Heart transplantation Multi drug resistant organisms Transplantation complications Transplantation outcome

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Septic cardiomyopathy phenotype in the critically ill may depend on antimicrobial resistance

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Journal of Intensive Medicine 2024年第3期4卷 355-361页

作者： Vasiliki Tsolaki kyriaki Parisi George E.Zakynthinos Efrosini Gerovasileiou Nikitas Karavidas Vassileios Vazgiourakis Epaminondas Zakynthinos Demosthenes Makris Intensive Care Unit University Hospital of LarissaUniversity of Thessaly Faculty of MedicineLarissaGreece Third Cardiology Clinic University of AthensSotiria HospitalAthensGreece Respiratory Department University Hospital of LarissaUniversity of Thessaly Faculty of MedicineLarissaGreece

Background Sepsis is a life-threatening organ dysfunction,and septic cardiomyopathy(SCM)may complicate the course of the *** with multidrug-resistant(MDR)pathogens has been linked with worse *** study aims to evaluate SCM in patients with infections caused by different antimicrobial-resistant *** This retrospective study included patients with sepsis/septic shock,hospitalized,and intubated in the intensive care unit of the University Hospital of Larissa between January 2022 and September 2023 with echocardiographic data during the first two days after infection *** patients were divided into two groups:non-MDR-SCM group and MDR-SCM *** cardiac function was compared between the two *** A total of 62 patients were included in the ***-four patients comprised the MDR-SCM and 18 the non-MDR-SCM ***-six patients(41.9%)presented with left ventricular(LV)systolic dysfunction,and≤35%right ventricular fractional area change(RVFAC)was present in 56.4%.LV systolic function was more severely impaired in the non-MDR-SCM group(left ventricular ejection fraction,35.8%±4.9%vs.45.6%±2.4%,P=0.049;LV outflow tract velocity time integral,[10.1±1.4]cm vs.[15.3±0.74]cm,P=0.001;LV-Strain,–9.02%±0.9%vs.–14.02%±0.7%,P=0.001).The MDR-SCM group presented with more severe right ventricular(RV)dilatation(right ventricular end-diastolic area/left ventricular end-diastolic area,0.81±0.03 vs.0.7±0.05,P=0.042)and worse RV systolic function(RVFAC,32.3%±1.9%vs.39.6%±2.7%,P=0.035;tricuspid annular plane systolic excursion,[15.9±0.9]mm vs.[18.1±0.9]mm,P=0.165;systolic tissue Doppler velocity measured at the lateral tricuspid annulus,[9.9±0.5]cm/s vs.[13.1±0.8]cm/s,P=0.002;RV-strain,–11.1%±0.7%vs.–15.1%±0.9%,P=0.002).Conclusion SCM related to MDR infection presents with RV systolic dysfunction predominance,while non-MDR-SCM is mainly depicted with LV systolic dysfunction impairment.

关键词： Septic cardiomyopathy Multidrug-resistant pathogen Left ventricular failure Right ventricular dysfunction Left/right ventricular longitudinal strain Ventriculoarterial Coupling

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Quality of life and functional capacity in patients after cardiac surgery intensive care unit

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World Journal of Cardiology 2024年第8期16卷 436-447页

作者： Vasiliki Raidou Katerina Mitete Christos Kourek Michael Antonopoulos Theodora Soulele kyriaki Kolovou Ioannis Vlahodimitris Ioannis Vasileiadis Stavros Dimopoulos Clinical Ergospirometry Exerciseand Rehabilitation LaboratoryDepartment of First Critical Care MedicineEvangelismos HospitalNational and Kapodistrian University of AthensAthens 10676Greece Cardiac Surgery Intensive Care Unit Onassis Cardiac Surgery CenterAthens 17674Greece

Coronary heart disease and aortic stenosis are prevalent cardiovascular diseases worldwide,leading to morbidity and *** artery bypass grafting(CABG)and surgical aortic valve replacement(SAVR)have therapeutic benefits,including improved postoperative quality of life(QoL)and enhanced patient functional capacity which are key indicators of cardiac surgery *** this article,we review the latest studies of QoL outcomes and functional capacity in patients who underwent cardiac *** standardized instruments are used to evaluate QoL and functional *** health status,age,length of intensive care unit stay,operative risk,type of procedure,and other pre-,intra-,and postoperative factors affect postoperative *** patients experience impaired physical status soon after cardiac surgery,but it improves in the following *** and SAVR are associated with increases of physical and mental health and functional capacity in the immediate postoperative and the long *** rehabilitation improves patient functional capacity,QoL,and frailty following cardiac surgery.

关键词： Quality of life Health-related quality of life Functional capacity Cardiac rehabilitation Cardiac surgery Coronary artery bypass grafting Heart valve surgery Heart valve replacement

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Radial and longitudinal density variations in Abies cephalonica and Pinus halepensis

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Journal of Forestry Research 2023年第3期34卷 853-863页

作者： Vasiliki Dimou Anna Tsaliki kyriaki Kitikidou Laboratory of Forest Technology Department of Forestry and Management of the Environment and Natural ResourcesDemocritus University of Thrace193 Pandazidou68200 OrestiadaPCGreece Laboratory of Forest Biometry-Biostatistics Department of Forestry and Management of the Environment and Natural ResourcesDemocritus University of Thrace193 Pandazidou68200 OrestiadaPCGreece

Basic wood density is an excellent indicator of quality,it is correlated with numerous physical,physiological and mechanical characteristics of a species and is a good descriptor of wood quality and important indicator of tree performance in community *** accurate method is thus needed to estimate wood *** standard way is to calculate the ratio of the oven-dried mass of a wood sample divided by its green volume,but wood characteristics within and between growth rings are highly variable;the density can vary in the longitudinal and the radial *** present study investigates the longitudinal and radial fl uctuations in the basic density of Abies cephalonica Loud and Pinus halepensis *** logs were cut from four diff erent trees for each species and 16 discs were formed(two discs from each log,one at the base and one at the top).Each disc was cut into cubes where their distance from the pith was measured and then sorted into 2 cm wide concentric *** results revealed a mild decreasing trend in basic density from the foot of the trunk upward for both *** pith in both species also seems to have a relatively high density,which in the immediate next growth rings decreases only to resume increasing toward the bark.

关键词： Aleppo pine Physical properties Specifi c gravity Wood quality

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Arsenic speciation in fish from Greek coastal areas

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Journal of Environmental Sciences 2017年第6期29卷 300-312页

作者： Ioanna Kalantzi kyriaki Mylona Katerina Sofoulaki Manolis Tsapakis Spiros A.Pergantis Institute of Oceanography Hellenic Centre for Marine Science(HCMR)PO Box 2214Heraklion 71003CreteGreece Environmental Chemical Processes Laboratory Department of ChemistryUniuersity of CreteHeraklion 71003CreteGreece

Arsenic speciation analysis was conducted on fish samples （sardine and anchovy） collected from six areas along the Greek coastline, i.e. Artemisium Straits, Thermaikos Gulf, Amvrakikos Gulf, Strymonian Gulf, Thracian Sea, and Elefsina Gulf. Total arsenic levels ranging from 11.8 to 62.6 mg As/kg dry weight were determined. Arsenobetaine, a non-toxic form of arsenic, was found to be the main arsenic species, present at 8.6 to 58.8 mg As/kg dry weight, accounting for 67--95% of the total arsenic. Also detected in all fish samples was dimethylarsinic acid, although at considerably lower concentrations, ranging from 0.072-3.956 mg As/kg dry weight. Monomethylarsonic acid was detected at low levels in all anchovy samples, and only in sardines from one area. Finally, inorganic arsenic in the form of arsenate was detected only in fish at one area, indicating the possible effect of an environmental parameter on its presence at detectable amounts. Statistical analysis revealed the environmental variables, such as salinity, total organic carbon and nitrogen, ammonium, phosphate, total phosphorus, dissolved oxygen and pressure index, are potentially correlated to As species concentrations. Furthermore, based on factor analysis, the biological parameters, such as fish weight, lipids, protein and ash content, that are correlated to As species concentrations of fish were also identified. The interrelationship of arsenobetaine and dimethylarsinic acid concentrations within each fish species was evaluated.

关键词： Arsenic speciationArsenobetaineDimethylarsinic acidArsenateFishEnvironmental and biologicalparameters

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Design and synthesis of thermally stable single atom catalysts for thermochemical CO_(2) reduction

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Journal of Energy Chemistry 2023年第11期86卷 246-262,I0006页

作者： Eswaravara Prasadarao Komarala Ayesha A.Alkhoori Xiaolong Zhang Hui-Ming Cheng kyriaki Polychronopoulou Department of Mechanical Engineering Khalifa University of Science and TechnologyMain CampusP.O.Box 127788Abu DhabiUnited Arab Emirates Center for Catalysis and Separations Khalifa University of Science and TechnologyP.O.Box 127788Abu DhabiUnited Arab Emirates Shenzhen Key Laboratory of Energy Materials for Carbon Neutrality Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055GuangdongChina Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016LiaoningChina

The continuous and excessive emission of CO_(2)into the atmosphere presents a pressing challenge for global sustainable *** response,researchers have been devoting significant efforts to develop methods for converting CO_(2)into valuable chemicals and *** conversions have the potential to establish a closed artificial carbon cycle and provide an alternative resource to depleting fossil *** the various conversion routes,thermochemical CO_(2)reduction stands out as a promising candidate for *** the realm of heterogeneous catalysis,single atom catalysts(SACs)have garnered significant *** utilization of SACs offers tremendous potential for enhancing catalytic *** achieve optimal activity and selectivity of SACs in CO_(2)thermochemical reduction reactions,a comprehensive understanding of key factors such as single atom metal-support interactions,chemical coordination,and accessibility of active sites is *** extensive research in this field,the atomic-scale reaction mechanisms in different chemical environments remain largely *** SACs have been found successful applications in electrochemical and photochemical CO_(2)reduction reactions,their implementation in thermochemical CO_(2)reduction encounters challenges due to the sintering and/or agglomeration effects that occur at elevated *** this review,we present a unique approach that combines theoretical understanding with experimental strategies to guide researchers in the design of controlled and thermally stable *** elucidating the underlying principles,we aim to enable the creation of SACs that exhibit stable and efficient catalytic activity for thermochemical CO_(2)reduction ***,we provide a comprehensive overview of recent literature on noble metal-and transition metal-based SACs for thermochemical CO_(2)*** current review is focused on certain CO_(2)-derived products involving one

关键词： CO_(2) utilization CO_(2) conversion SACs Added-value products Thermochemical catalysis

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Importance of serum phosphate in elderly patients with diabetes mellitus

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World Journal of Diabetes 2020年第10期11卷 416-424页

作者： Vaia D Raikou Despina kyriaki Sotiris Gavriil Department of Nephrology Doctors’HospitalAthens 11257Greece Department of Nuclear Medicine General Hospital“LAΪKO”Athens 11527Greece Department of Bariatric Surgery Doctors’HospitalAthens 11257Greece

BACKGROUND Metabolic disturbances including changes in serum calcium,magnesium or phosphate(P)influence the prevalence of type 2 diabetes mellitus(DM).We assessed the importance of serum P in elderly patients with type 2 DM vs nondiabetes mellitus(non-DM)in relation to renal *** To determine the association between serum P and serum glucose or insulin resistance in diabetic and non-diabetic *** One hundred-ten subjects with a mean age of 69.02±14.3 years were ***-nine of the participants had type 2 DM(26.4%).The incidence of hypertension,smoking and receiving vitamin D(vitD)derivates were *** participants were classified by both estimated glomerular filtration rate(eGFR)and albuminuria categories according to the Kidney Disease Improving Global Outcomes 2012 *** We divided the patients in two groups according to the P cut-off point related to DM value.A comparison between high and low P showed that body mass index 30.2±6.3 vs 28.1±4.6(P=0.04),mean glucose 63.6 vs 50.2(P=0.03),uric acid 6.7±1.6 vs 6.09±1.7(P=0.05),mean intact-parathyroid hormone 68.06 vs 47.4(P=0.001),systolic blood pressure 147.4±16.7 vs 140.2±16.1(P=0.02),mean albuminuria 63.2 vs 50.6(P=0.04)and eGFR 45.6±22.1 vs 55.4±21.5(P=0.02)were significantly different.χ^2 tests showed a significant association between high P and DM,hypertension,receiving vitD,smoking and eGFR stage(χ^2=6.3,P=0.01,χ^2=3.9,P=0.03,χ^2=6.9,P=0.009,χ^2=7.04,P=0.01 andχ^2=7.36,P=0.04,respectively).The adjusted model showed that older age,female gender and increased body mass index were significant predictors of type 2 DM when entering the *** High serum P contributes to vascular and metabolic disturbances in elderly patients with type 2 DM and renal impairment.

关键词： Serum phosphate Diabetes mellitus Renal disease Old age Albuminuria Vitamin D

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