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Impact of the antiproliferative agent ciclopirox olamine treatment on stem cells proteome

World Journal of Stem Cells 2013年第1期5卷 9-25页

作者： Gry H Dihazi asima Bibi Olaf Jahn Jessica Nolte Gerhard A Mueller Wolfgang Engel Hassan Dihazi Department of Nephrology and Rheumatology Georg-August University GoettingenD-37075 GoettingenGermany Proteomics Group Max-Planck-Institute of Experimental MedicineD-37075 GoettingenGermany Deutsche Forschungsgemeinschaft Research Center for Molecular Physiology of the Brain D-37073 Institute of Human Genetics Georg-August University GoettingenD-37073 GoettingenGermany

AIM:To investigate the proteome changes of stem cells due to ciclopirox olamine (CPX) treatment compared to control and retinoic acid treated cells. METHODS:Stem cells (SCs) are cells, which have the ability to continuously divide and differentiate into various other kinds of cells. Murine embryonic stem cells (ESCs) and multipotent adult germline stem cells (maG-SCs) were treated with CPX, which has been shown to have an antiproliferative effect on stem cells, and compared to stem cells treated with retinoic acid (RA),which is known to have a differentiating effect on stem cells. Classical proteomic techniques like 2-D gel electrophoresis and differential in-gel electrophoresis (DIGE) were used to generate 2D protein maps from stem cells treated with RA or CPX as well as from non-treated stem cells. The resulting 2D gels were scanned and the digitalized images were collated with the help of Delta 2D software. The differentially expressed proteins were analyzed by a MALDI-TOF-TOF mass spectrometer, and the identified proteins were investigated and categorized using bioinformatics. RESULTS:Treatment of stem cells with CPX, a synthetic antifungal clinically used to treat superficial mycoses, resulted in an antiproliferative effect in vitro, without impairment of pluripotency. To understand the mechanisms induced by CPX treatments which results in arrest of cell cycle without any marked effect on pluripotency, a comparative proteomics study was conducted. The obtained data revealed that the CPX impact on cell proliferation was accompanied with a significant alteration in stem cell proteome. By peptide mass fingerprinting and tandem mass spectrometry combined with searches of protein sequence databases, a set of 316 proteins was identified, corresponding to a library of 125 non-redundant proteins. With proteomic analysis of ESCs and maGSCs treated with CPX and RA, we could identify more than 90 single proteins, which were differently expressed in both cell lines. We cou

关键词： Stem cells Differentiation Hypusination Ciclopirox olamine Proteomics Retinoic acid

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磁控溅射用于细微颗粒涂镀功能薄膜的新技术

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辽宁科技大学学报 2017年第2期40卷 126-132页

作者： Peter J Kelly David Sawtell Glen T West May Azzawi asima Farooq Marina Ratova 英国曼彻斯特城市大学科学与工程学院英国曼彻斯特M15GD

磁控溅射是一项成熟的技术,可用在各种基体上沉积高质量金属及陶瓷涂层。然而,此项技术一般并不适于涂镀微粒粒度为nm或μm到100μm的细微粒涂层。本文详述了一种可涂镀微粒粒径在上述范围的均匀功能薄膜的技术。在磁场下面安放的一个... 详细信息

磁控溅射是一项成熟的技术,可用在各种基体上沉积高质量金属及陶瓷涂层。然而,此项技术一般并不适于涂镀微粒粒度为nm或μm到100μm的细微粒涂层。本文详述了一种可涂镀微粒粒径在上述范围的均匀功能薄膜的技术。在磁场下面安放的一个碗状容器内放有微粒子,容器可以振动可使粒子均匀地振出碗边沉落到真空腔,薄膜沉积工艺为反应或非反应脉冲磁控溅射。Ti,Ti O2,Sn及Sn O2从单磁场源沉积,而Bi/W的氧化物从双磁场源沉积。用SEM,TEM和EDX,以及其它与应用相关的技术表征所沉积的微粒,以表明此项技术的潜在功能。

关键词：功能薄膜磁控溅射粒子沉积振动

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