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Analysis of promoter activity reveals that GmFTL2 expression differs from that of the known Flowering Locus T genes in soybean

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The Crop Journal 2017年第5期5卷 438-447页

作者： Limin Liu Xiaomei Zhang Fulu Chen asia adam elzamzami mahi Xiaoxia Wu Qingshan Chen Yong-Fu Fu College of Agriculture Northeast Agricultural University MOA Key Lab of Soybean Biology (Beijing) National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural Sciences

Regulation of flowering is one of the key issues in crop yield. The Floiuering Loews T(FT) gene is a well-known florigen, which integrates various signals from multiple flowering-regulation pathways to initiate flowering. We previously reported that there are at least six FT genes(GmFTLl-6) in soybean displaying flowering activity. However, the individual functions of genes GmFTLl-6 remain to be identified. In this study, we cloned the GmFTL2 promoter(GmFTLpro) from soybean(Glycine max) cultivar Tianlong 1 and analyzed its motifs bioinformatically and its expression patterns using both a transgenic approach and quantitative RT-PCR(qRT-PCR). In GmFTLpro::GUS transgenic lines, GUS signals were enriched in cotyledons, hypocotyledons, pollen, embryos, and root tips in a photoperiod-independent manner. qRT-PCR confirmed the GUS reporter results. Our results suggest that GmFTL2 expression is regulated by developmental and tissue-specific clues and plays roles in seedling establishment and the development of micro game tophytes, embryos, and roots.

关键词： Flowering Locus T Cotyledon Microgametophyte Embryo Root tip

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The Origin and Diversification of Plant Family Dipterocarpaceae

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Open Journal of Geology 2019年第10期9卷 593-596页

作者： mahi Bansal Shivaprakash K. Nagaraju Vandana Prasad Terminal Cretaceous-Cenozoic Ecosystem Laboratory Birbal Sahni Institute of Palaeosciences Lucknow India Centre for Structural and Functional Genomics Biology Department Concordia University Montreal Canada The Nature Conservancy Centre India (TNCIndia) New Delhi Delhi India

The Dipterocarpaceae plant family, that shows a disjunct distribution in Gondwanan continents and Southeast asia, is a dominant constituent of the tropical rain forests of Southeast asia. The high species diversity of Dipterocarpaceae in SE asian rain forests suggests its origin from SE asia. However, its fossil history is much younger, from Oligocene, from the region. Based on the pollen fossil records from the late Cretaceous-early Paleogene sedimentary sequences of Indian subcontinent and the contemporaneous distribution of its extant taxa, evolutionary history of Dipterocarpaceae has been traced. The study suggests a West Gondwanan origin for this family. Present study also provides first evidence of Dipterocarpaceae genus Vateriopsis (endemic in Seychelles) type fossil pollen record from the late Cretaceous and early Palaeogene sedimentary sequences of western Indian margin.

关键词： Fossil Vateriospis Pollen Cretaceous Dipterocarpaceae

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