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Tri-milR29 from Trichoderma breve Targets Chitin Synthase Gene in Botrytis cinerea and its Enhanced Disease Control Efficacy through Nanodelievery-base Application

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Tri-milR29 from Trichoderma breve Targets Chitin Synthase Ge...

中国植物病理学会2024年学术年会

作者：刘震 Raja asad ali Khan WANG Rui LI Yuejiao 刘铜 School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication) Hainan University Key Laboratory of Green Prevention and Control of Tropical Diseases and Pests Ministry of Education (School of Tropical Agriculture and Forestry)Hainan University Engineering Center of Agricultural Microbial Preparation Research and Development of Hainan (Hainan University)

In the process of mycoparasitism,a form of biological control that one fungus attacking another fungus,the exchange of biologically active molecules such as RNAs may occur,playing an important role in *** these molecules,microRNAs(miRNAs) can inhibit translation and regulate gene expression at the post-transcriptional level through complementary base pairing,thereby degrading target mRNAs to prevent ***,a well-known mycoparasitic fungus,is one of the most studied biocontrol *** recent years,small RNAs(named milRNA) have also been discovered in ***,the role of Trichoderma RNAs in their mycoparasitic activity and their targets in pathogenic fungi still needs further *** study verified the cross-species regulatory role of Trichoderma-T069 miRNA,named *** demonstrated that Tri-milR29 from T069 was transferred to ***,a pathogenic fungus,where it targeted the chitin synthase gene,BcCHS7,resulting in a marked decrease in its ***-species transportation of sRNAs may occur through extracellular vesicles(EVs) ***,more research is needed on EVs in *** a series of experiments,we verified that the Tri-milR29 from T069 was transferred to *** through the EVs *** sRNA can effectively prevent and control plant diseases by targeting specific genes in pathogenic microbes through RNA ***,their susceptibility to nuclease degradation,off-target effects,and their low absorption efficiency by target pathogens limit their practical ***,the development of suitable delivery systems to protect and deliver sRNA is *** developed a carbon nanomaterial-based delivery system that enhances the stability and absorption of TrimilR29 by ***,increasing antifungal *** nano-delivered Tri-milR29 significantly lowered the disease incidence caused by *** on various fruits and tomato plants.

关键词： microRNA extracellular vesicles biocontrol mycoparasitism pathogen

The combined application of a biocontrol agent Trichoderma asperellum SC012 and Hymexazol reduced the fungicide dose to control Fusarium wilt in cowpea

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The combined application of a biocontrol agent Trichoderma a...

中国植物病理学会2021年学术年会

作者： RAJA asad ali KHAN WANG Weiwei ZHANG Fengtao LIU Zhen 刘铜 College of Plant Protection Hainan University

Fungicides use needs to be gradually reduced because of its adverse effect on human health and the ***,an integrated approach combining fungicides with biological control agents（BCAs） is put forward to reduce the fungicide doses,there by minimizing the risks associated with chemical *** this study,the combined application of a BCA Trichoderma and a fungicide hymexazol was used to manage the cowpea wilt disease caused by Fusarium *** Trichoderma strain SC012,which is resistant to hymexazol,was screened out and identified as *** SC012 showed hyperparasitism to *** and could penetrate and encircle the hyphae of pathogen on a medium amended with hymexazol or *** combined with hymexazol,the population density in the rhizosphere soil of cowpea showed no significant difference compared with the treatment Trichoderma used *** the concentration of *** SC012 or hymexazol was halved,their combined application could control cowpea wilt disease more effectively than their individual *** study showed that the combination of Trichoderma and chemical fungicide could reduce the use of chemical,which is eco-friendly and may be an important part of integrated control of *** in cowpea.

关键词： Trichoderma asperellum hymexazol Fusarium wilt cowpea

巴基斯坦萨戈达地区危害桔园的桃实蝇时空分布（英文）

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昆虫学报 2017年第12期60卷 1457-1466页

作者： Muhammad ARIF Muhammad SIDDIQUE AASI Muhammad FAROOQ Habib ali Saif UL ISLAM Muhammad asad Muhammad SHAKEEL 吴祖建福建农林大学植物保护学院福州350002 Department of Pest Warning and Quality Control of Pesticides District Sargodha Cotton Research Station FaisalabadPakistan 华南农业大学农学院广州510642

【目的】本研究旨在监测2009-2011年Tehsil Sargodha 7个地点(Sargodha-Ⅰ,Sargodha-Ⅱ,Bhagtanwala,Sakessar,Chak#75-SB,Chak#46-SB和Chak#104-NB)中桃实蝇Bactrocera zonata优势种的种群变化情况。【方法】通过甲基丁香酚信息素诱捕器诱捕桃实蝇,每周统计记录桃实蝇种群发生情况,诱捕器每两周加药一次。【结果】结果表明,在调查的3年(2009-2011)中,Sargodha-Ⅰ的桃实蝇种群多度最高(分别为53.67,45.82和45.47头/诱捕器),其次为Sakessar(分别为41.13,33.87和35.75头/诱捕器),而Chak#75-SB的种群多度最低(分别为15.78,19.18和19.15头/诱捕器)。每年桃实蝇发生最高峰出现在4月(分别为76.08,71.94和61.51头/诱捕器),其次为5月(分别为60.74,52.63和64.00头/诱捕器),而在2月和10月发生量最低。另外,桃实蝇种群多度与最高和最低气温呈较强的正相关,而与相对湿度和降雨量呈负相关。同样,回归系数表明,最高气温是影响桃实蝇种群发生的主要因素,而降雨量的影响最小。【结论】应当全年对桃实蝇进行定期监测,由于气象因子严重影响桃实蝇的种群发生情况,因此尤其应监测其在4-5月温度开始上升时的发生情况。

关键词：实蝇科桃实蝇种群动态气象因子柑橘信息素诱捕器

Proliferation, Multiplication and Improvement of Micro-Propagation System for Mass Clonal Production of Rose through Shoot Tip Culture

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American Journal of Plant Sciences 2018年第2期9卷 296-310页

作者： Allah Jurio Khaskheli Muhammad Ibrahim Khaskheli Maqsood Ahmad Khaskheli Tahmina Shar Waqas Ahmad Umed ali Lighari Muhammad Azeem Khaskheli asad ali Khaskheli Faisal Hayat Makan Department of Biotechnology Sindh Agriculture University Tando Jam Pakistan Department of Plant Protection Sindh Agriculture University Tando Jam Pakistan State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou China Collage of Agriculture and Biotechnology China Agriculture University Beijing China Institute of Pomology Chinese Academy of Agriculture Sciences Xingcheng China Department of Animal Nutrition Sindh Agriculture University Tando Jam Pakistan

Present study was conducted to assess the regeneration potential and producing mass-clonal seedlings of Rose through shoot tip culture. A total of 40 explants were cultured on each of basal medium supplemented with different concentrations of BAP, NAA and GA. The observations on the survival rate, days taken to initiate the shoots, total number of shoots and length of shoots, initiation of roots, total number of root, length of roots and number of leaves were investigated. Rose regenerated on MS-Basal medium (control) without addition of growth hormones showed significantly (P < 0.05) lower survival rate and did not show shoots up to the end of experiments. MS-Basal medium supplemented with different concentrations of BAP, NAA and GA showed increasing rate of survival. MS-Basal medium supplemented with highest BAP, NAA and GA concentration (MS-SV) has taken least time to initiate the shoots, whilst supplemented in concentrations of 0.5 and/or 1.0 mg/L, respectively revealed more time. Rooted plants were transplanted into the substrate and acclimatized in the laboratory greenhouse (humid cavity). The acclimatization in the humid cavity showed optimistic effect on the number of survived plants.

关键词： Rose Tissue Culture Explants BAP NAA and GA

Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds

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Burns & Trauma 2021年第1期9卷 272-287页

作者： Maryam Azam Hafiz Ghufran Hira Butt Azra Mehmood Ramla Ashfaq asad M.Ilyas Muhammad R.Ahmad Sheikh Riazuddin National Centre of Excellence in Molecular Biology 87-West Canal Bank RoadUniversity of the PunjabLahorePakistan Jinnah Burn and Reconstructive Surgery Centre Maulana Shaukat Ali RdQuaid-i-Azam CampusLahorePakistan

Background:Following recent findings from our group that curcumin preconditioning augments the therapeutic efficacy of adipose-derived stem cells in the healing of diabetic wounds in rats,we aimed to investigate the regenerative effects of curcumin preconditioned adipose-derived mesenchymal stem cells(ASCs)for better recovery of acid inflicted burns in this ***:ASCs were preconditioned with 5μM curcumin for 24 hours and assessed for proliferation,migration,paracrine release potential and gene expression comparative to naive ***,the healing capacity of curcumin preconditioned ASCs(Cur-ASCs)versus naive ASCs was examined using acidic wounds in *** this,acid inflicted burns of 20 mm in diameter were made on the back of male Wistar ***,2×10^(6) cells of Cur-ASCs and naive ASCs were intradermally injected in the wound periphery(n=6)for comparison with an untreated saline ***-transplantation,wounds were macroscopically analysed and photographed to evaluate the percentage of wound closure and period of *** wound biopsies were excised and used for histological evaluation and expression analysis of wound healing markers at molecular level by quantitative PCR and western ***:We found that Cur-ASCs exhibited greater proliferation,migration and paracrine potential in ***,Cur-ASCs showed more effective recovery than naive ASCs as exhibited by gross morphology,faster wound closure and earlier ***’s trichrome and hematoxylin and eosin staining demonstrated the improved architecture of the healing burns,as evidenced by reduced infiltration of inflammatory cells,compact collagen and marked granulation in Cur-ASC treated *** these findings,molecular assessment showed significantly reduced expressions of pro-inflammatory factors(interleukin-1 beta,interleukin-6,tumor necrosis factor alpha)a with striking upsurge of an oxidative marker(superoxide dismutase 1),p

关键词： Adipose-derived stem cells Curcumin preconditioning Acidic burn Wound healing

巴基斯坦锂、铍地球化学分布特征与成矿远景区优选

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地学前缘 2024年

作者：洪俊 Tahseenullah Khan Yasir Shaheen Khalil 马中平张晶王志华张辉善张海迪 asad ali Narejo 自然资源部岩浆作用成矿与找矿重点实验室中国地质调查局西安地质调查中心巴基斯坦巴利亚大学地球与环境科学学院巴基斯坦地质调查局

锂、铍金属是世界战略关键金属资源，在新材料和信息技术等新兴产业具有不可替代的重大用途。巴基斯坦位于欧亚、印度和阿拉伯三大板块汇聚部位，拥有优越的锂、铍成矿条件，但由于地质工作程度偏低，目前锂、铍金属资源潜力不明。低密... 详细信息

锂、铍金属是世界战略关键金属资源，在新材料和信息技术等新兴产业具有不可替代的重大用途。巴基斯坦位于欧亚、印度和阿拉伯三大板块汇聚部位，拥有优越的锂、铍成矿条件，但由于地质工作程度偏低，目前锂、铍金属资源潜力不明。低密度地球化学调查是研究锂、铍元素空间分布、快速圈定成矿远景区的有效方法。本文利用巴基斯坦1:100万低密度地球化学调查成果，阐述了巴基斯坦锂、铍元素地球化学背景，结合已有锂、铍矿床成矿地质背景分析，圈定了锂、铍成矿远景区，为锂、铍稀有金属找矿勘查提供依据。巴基斯坦全境基岩出露区水系沉积物中锂的含量在1.56μg/g ～118.2μg/g之间，平均含量为20.06 μg/g。铍总体变化范围为0.07μg/g ～7.16μg/g，平均值为μg/g。根据92%累频共圈定18个锂地球化学异常和12个铍地球化学异常，并优选找矿远景区。其中，喀喇昆仑地体和喜马拉雅造山带作为伟晶岩型锂矿的找矿远景区，查盖岩浆弧东作为盐湖卤水型锂矿的找矿远景区，喀喇昆仑地体是寻找伟晶岩型锂、铍矿床最有利的地区。

关键词：锂铍稀有金属资源潜力地球化学调查远景区预测巴基斯坦

巴基斯坦穆斯林巴赫铬铁矿床成矿环境、地质特征及控矿因素

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地质论评 2016年第B11期62卷 161-162页

作者：洪俊 TAHSEENULLAH Khan 张晶张辉善杨博 asadA li 国土资源部岩浆作用成矿与找矿重点实验室中国西安710054 中国地质调查局西安地质调查中心中国西安710054 巴基斯坦Bahria大学地球与环境学院巴基斯坦伊斯兰堡44000 巴基斯坦地质调查局巴基斯坦伊斯兰堡44000

巴基斯坦境内蛇绿岩广泛分布，且大多发育铬铁矿化。穆斯林巴赫铬铁矿床位于巴基斯坦中部，是新特提斯缝合带中大型豆荚状铬铁矿床之一，目前已经发现数百个矿体，储量超过4000kt，是巴基斯坦境内最重要的铬铁矿产地。张洪瑞等（2013）... 详细信息

巴基斯坦境内蛇绿岩广泛分布，且大多发育铬铁矿化。穆斯林巴赫铬铁矿床位于巴基斯坦中部，是新特提斯缝合带中大型豆荚状铬铁矿床之一，目前已经发现数百个矿体，储量超过4000kt，是巴基斯坦境内最重要的铬铁矿产地。张洪瑞等（2013）总结了中部瓦济里斯坦-贝拉蛇绿岩带的地质特征和穆斯林巴赫铬铁矿床的产出特征，认为穆斯林巴赫铬铁矿床成因仍存在争议，其形成构造环境、控矿因素有待于进一步研究。

关键词：控矿因素铬铁矿穆斯林巴赫巴基斯坦

Smooth Sliding Mode Control for PEM Fuel Cell System

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Smooth Sliding Mode Control for PEM Fuel Cell System

第24届中国控制与决策会议

作者： S. Saqib H. Rizvi A. I. Bhatti Qudrat Khan Qadeer Ahmad asad Hameed Zahid Butt CASPR Dept.of Electronics Engineering Mohammad Ali Jinnah University

A smooth sliding mode control （SSMC） is applied to tackle the problem of PEM fuel cell system output voltage stabilization problems along with controlling oxygen excess ratio. The oxygen excess ratio is controlled in the inner control loop using PID while the regulation of output voltage is being treated in the outer loop through smooth sliding mode control. The control is applied on the validated nonlinear model of the PEM fuel cell system. The simulation results show that the scheme with SSM control provides same performance as compared to higher order sliding mode control （Super twisting Algorithm） in output voltage stabilization and also in minimizing the fuel consumption.

关键词： Smooth Sliding Mode Control PEM Fuel Cell System

木霉诱导的根际微生物群重组及合成群落对黄瓜枯萎病和生长的影响

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木霉诱导的根际微生物群重组及合成群落对黄瓜枯萎病和生长的影响

中国植物病理学会2024年学术年会

作者：张漫漫王睿 Raja asad ali Khan 战鑫任森姜浩楠吴英姑杨繁兴于笑丽缪卫国刘铜海南大学热带农林学院海南省绿色农用生物制剂创制工程研究中心

与植物根系相关的细菌群落在抑制土传病害中起着重要作用,多品种有益微生物组合可以增强病害抑制作用。本研究用棘孢木霉FJ035处理连作模式下黄瓜根际,探究了其对连作黄瓜土壤中微生物群落的变化、对黄瓜枯萎病的防效及对黄瓜植株生长... 详细信息

与植物根系相关的细菌群落在抑制土传病害中起着重要作用,多品种有益微生物组合可以增强病害抑制作用。本研究用棘孢木霉FJ035处理连作模式下黄瓜根际,探究了其对连作黄瓜土壤中微生物群落的变化、对黄瓜枯萎病的防效及对黄瓜植株生长的影响。结果表明,木霉影响了黄瓜连作土壤中微生物群落变化,从而显著促进了黄瓜植株的生长,增强了黄瓜对黄瓜枯萎病菌SSCFo1的根际免疫效应。同时,根据宏基因组和培养组联合分析及菌群回接实验结果构建了由FJ035和来自11个细菌属的30株菌株组成的细菌-真菌合成群落TB11。接种SSCFo1的黄瓜植株经TB11处理后,枯萎病发病率降低了70.0%,鲜重增加了64.59%。将合成菌群TB11进一步简化为由FJ035和5属6株菌组成的TB5简化菌群。TB5在病害控制和促进生长方面与Tb11类似。TB5中的细菌菌株兼具抑制SCCFo1的生长和溶解土壤养分的多重功能,在抑制黄瓜枯萎病的同时促进了黄瓜植株的生长,还增加了黄瓜植株抗氧化、防御相关酶和生长激素相关基因的表达。这些发现阐明了有益的生防真菌与本地微生物之间的相互作用来提高生物防治稳定性,以保护作物免受病原体侵害,利用有益的合成微生物组合来支持可持续农业管理系统。

关键词：细菌-真菌合成群落抗病性抗真菌活性植物微生物互作