Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds
作者机构：National Centre of Excellence in Molecular Biology87-West Canal Bank RoadUniversity of the PunjabLahorePakistan Jinnah Burn and Reconstructive Surgery CentreMaulana Shaukat Ali RdQuaid-i-Azam CampusLahorePakistan
出 版 物：《Burns & Trauma》 (烧伤与创伤（英文）)
年 卷 期：2021年第9卷第1期
页 面：272-287页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by research grants from the Higher Education Commission of Pakistan
主 题：Adipose-derived stem cells Curcumin preconditioning Acidic burn Wound healing
摘 要：Background:Following recent findings from our group that curcumin preconditioning augments the therapeutic efficacy of adipose-derived stem cells in the healing of diabetic wounds in rats,we aimed to investigate the regenerative effects of curcumin preconditioned adipose-derived mesenchymal stem cells(ASCs)for better recovery of acid inflicted burns in this ***:ASCs were preconditioned with 5μM curcumin for 24 hours and assessed for proliferation,migration,paracrine release potential and gene expression comparative to naive ***,the healing capacity of curcumin preconditioned ASCs(Cur-ASCs)versus naive ASCs was examined using acidic wounds in *** this,acid inflicted burns of 20 mm in diameter were made on the back of male Wistar ***,2×10^(6) cells of Cur-ASCs and naive ASCs were intradermally injected in the wound periphery(n=6)for comparison with an untreated saline ***-transplantation,wounds were macroscopically analysed and photographed to evaluate the percentage of wound closure and period of *** wound biopsies were excised and used for histological evaluation and expression analysis of wound healing markers at molecular level by quantitative PCR and western ***:We found that Cur-ASCs exhibited greater proliferation,migration and paracrine potential in ***,Cur-ASCs showed more effective recovery than naive ASCs as exhibited by gross morphology,faster wound closure and earlier ***’s trichrome and hematoxylin and eosin staining demonstrated the improved architecture of the healing burns,as evidenced by reduced infiltration of inflammatory cells,compact collagen and marked granulation in Cur-ASC treated *** these findings,molecular assessment showed significantly reduced expressions of pro-inflammatory factors(interleukin-1 beta,interleukin-6,tumor necrosis factor alpha)a with striking upsurge of an oxidative marker(superoxide dismutase 1),p
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