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Virologica Sinica 2010年第5期25卷 352-360页

作者： Manoj Kumar Sukdeb nandi Sunil Chidri Virology Laboratory Centre for Animal Disease Research and DiagnosisIndian Veterinary Research Institute

A polyclonal antibody-based antigen-capture ELISA （AC-ELISA） has been developed for detection of Canine parvovirus （CPV） antigens in faecal samples of dogs. The assay uses rabbit anti-CPV polyclonal antibody as the capture antibody, guinea pig anti-CPV polyclonal antibody as tracing antibody and anti-guinea pig HRPO conjugate as the detection system. The optimum dilution of the capture antibody and the tracing antibody capable of detecting the CPV-2 antigens was found to be 1：1 600 and 1：400, respectively, in the check-board titration. In this study, a total of 152 samples （129 faecal samples and 23 cell culture supernatant） were tested both by AC-ELISA and by polymerase chain reaction （PCR）. Of the samples tested, 69 and 78 samples were found positive by AC-ELISA and PCR, respectively. The AC-ELISA had relative sensitivity, relative specificity and accuracy of 88.4%, 100.0% and 91.4% respectively. The analytical sensitivity of AC-ELISA was estimated to be 102.8 TCID50/mL whereas PCR sensitivity was 100.8 TCIDs0/mL. The AC-ELISA is a simple, quick and reliable method for screening large numbers of faecal samples of dogs suspected of CPV infection.

关键词： Canine parvovirus (CPV) Polyclonal antibody Antigen-capture ELISA(AC-ELISA)

Allelopathic effects of Mikania cordata on forest and agricultural crops in Bangladesh

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Journal of Forestry Research 2016年第1期27卷 155-159页

作者： M.K.Hossain S.Anwar R.nandi Institute of Forestry and Environmental Sciences University of Chittagong

Mikania cordata, an invasive weed is becom- ing a problem in the secondary degraded forests and plantations of Bangladesh, not only competing with but also releasing allelochemicals to cultivated crops. Allelo- pathic effects of the leaves of M. cordata were investigated through an experiment in the green house of the Institute of Forestry and Environmental Sciences, University of Chit- tagong, Bangladesh. Five economically important agricul- tural crops （Oryza sativa L., Triticum aestivum L., Vigna sinensis L., Abelmoschus esculentus L. and Amaranthus tricolor L.） and three forest crops （Acacia auriculiformis A. Cunn. ex Benth. ＆ Hook, Albizia procera （Roxb.） Benth., and Paraserianthes falcataria （L.） Nielson） were used as bioassay species. Experiments were conducted on trays at an average room temperature 27 ~C. The effects of dif- ferent concentrations of leaf extracts were compared to controls. Water soluble allelochemicals of M. cordata inhibited the germination and initial growth of the tested agricultural and forest crops. Germination percent of forest crops declined with increasing concentration of extracts. The inhibitory effect on root elongation of forest crops was pronounced, whereas a stimulatory effect was found on P. falcataria up to a concentration of 25 %. Abelmoschus esculentus showed poor germination （%） and low shoot development （0.5 cm） and no development of taproot or lateral roots at treatment of 100 % concentration. Theinhibitory effect was proportional to the concentrations of the extracts with higher concentrations producing stronger inhibitory effects. Lower concentrations showed some stimulatory effect. The inhibitory effect was pronounced in root and lateral root development rather than germination or shoot development of the crops. Mikania cordata should be eradicated for healthy growth and development of the forest and adjacent agricultural crops.

关键词： Mikania cordata Allelopathy Agriculturalcrops Forest crops Germination Growth inhibition

Effect of pyroxenite and olivine minerals as source of MgO in hematite pellet on improvement of metallurgical properties

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Journal of Central South University 2015年第9期22卷 3302-3310页

作者： Jagannath Pal Satadal Ghorai Bikash nandi Tapas Chakraborty Goutam Das T.Venugopalan CSIR-National Metallurgical Laboratory Tata Steel

Pelletization of hematite ore requires high fineness and very high induration temperature（~1325 ℃） owing to its poor diffusion bonding unlike magnetite ore. Further, high-alumina hematite pellets show very high reduction degradation index（RDI） during low temperature（500-650 ℃） reduction due to their volume expansion and lattice distortion. Noamundi（India） hematite ore contains very high Al2O3（2.3%） with adverse ratio of alumina to silica（~2） for which, it shows very high RDI. In this work, the acid pellets prepared from Noamundi ore fines of optimum Blaine fineness show good cold crushing strength（CCS）. However, it shows very high RDI（77%）. In order to reduce RDI, Mg O in form of two different gangue-containing fluxes, such as pyroxenite and olivine in varying quantities has been added. The optimum requirement and performance of these fluxes has been examined and compared. Both pyroxenite and olivine fluxed pellets show significant lowering of RDI（26% and 23%, respectively） and improvement of other properties, viz CCS, swelling indices etc with good reducibility（70%-77%）. Finally, a good quality acidic hematite pellet was developed from high-alumina ore without using any lime which is very important charge material in combination of basic sinter in blast furnace.

关键词： hematite pellet high-alumina ore reduction degradation index(RDI) pyroxenite flux olivine flux effect of MgO

In-Situ Hydrothermal Synthesis of Bi–Bi2O2CO3 Heterojunction Photocatalyst with Enhanced Visible Light Photocatalytic Activity

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Nano-Micro Letters 2017年第2期9卷 58-67页

作者： Prasenjit Kar Tuhin Kumar Maji Ramesh nandi Peter Lemmens Samir Kumar Pal Department of Chemical Biological and Macromolecular Sciences S.N.Bose National Centre for Basic SciencesBlock JDSector ⅢSaltLakeKolkata 700106India Institute for Condensed Matter Physics TU BraunschweigMendelssohnstraße 338106 BrunswickGermany Laboratory for Emerging Nanometrology TU BraunschweigBrunswickGermany

Bismuth containing nanomaterials recently received increasing attention with respect to environmental applications because of their low cost, high stability and nontoxicity. In this work, Bi–Bi_2O_2CO_3 heterojunctions were fabricated by in-situ decoration of Bi nanoparticles on Bi_2O_2CO_3 nanosheets via a simple hydrothermal synthesis approach. X-ray diffraction(XRD), scanning electron microscopy(SEM), transmission electron microscopy(TEM) and high-resolution TEM(HRTEM) were used to confirm the morphology of the nanosheet-like heterostructure of the Bi–Bi_2O_2CO_3 composite. Detailed ultrafast electronic spectroscopy reveals that the in-situ decoration of Bi nanoparticles on Bi_2O_2CO_3 nanosheets exhibit a dramatically enhanced electron-hole pair separation rate, which results in an extraordinarily high photocatalytic activity for the degradation of a model organic dye, methylene blue(MB) under visible light illumination. Cycling experiments revealed a good photochemical stability of the Bi–Bi_2O_2CO_3 heterojunction under repeated irradiation. Photocurrent measurements further indicated that the heterojunction incredibly enhanced the charge generation and suppressed the charge recombination of photogenerated electron-hole pairs.

关键词： Bi nanoparticles Bi–Bi2O2CO3 nanosheets Heterojunction Hydrothermal method Charge separation Visible light photocatalytic activity

Configuration-Sensitive Molecular Sensing on Doped Graphene Sheets

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Nano Research 2010年第7期3卷 472-480页

作者： John Russell Petr král Department of Chemistry University of Illinois at ChicagoChicagoIL 60607USA

We show by molecular dynamics simulations that configuration-sensitive molecular spectroscopy can be realized on optimally doped graphene sheets vibrated by an oscillatory electric *** selectivity of the spectroscopy is achieved by maximizing Coulombic binding between the detected molecule and a specific nest,formed for this molecule on the graphene sheet by substituting selected carbon atoms with boron and nitrogen *** can detect binding of different isomers to the nest from the frequency shifts of selected vibrational modes of the combined *** an illustrative example,we simulate detection of hexanitrostilbene enantiomers in chiral nests formed on graphene.

关键词： Molecular sensing enantiomer graphene functionalization molecular dynamics

Systematic bio‑fabrication of aptamers and their applications in engineering biology

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Systems Microbiology and Biomanufacturing 2023年第2期3卷 223-245页

作者： Rongfeng Cai Xin Chen Yuting Zhang Xiaoli Wang nandi Zhou The Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Aptamers are single-stranded DNA or RNA molecules that have high affinity and selectivity to bind to specific *** to antibodies,aptamers are easy to in vitro synthesize with low cost,and exhibit excellent thermal stability and *** these features,aptamers have been widely used in biology and medicine-related *** the meantime,a variety of systematic evolution of ligands by exponential enrichment(SELEX)technologies have been developed to screen aptamers for various *** to the characteristics of targets,customizing appropriate SELEX technology and post-SELEX optimization helps to obtain ideal aptamers with high affinity and *** this review,we first summarize the latest research on the systematic bio-fabrication of aptamers,including various SELEX technologies,post-SELEX optimization,and aptamer modification *** procedures not only help to gain the aptamer sequences but also provide insights into the relationship between structure and function of the *** latter provides a new perspective for the systems bio-fabrication of ***,on this basis,we review the applications of aptamers,particularly in the fields of engineering biology,including industrial biotechnology,medical and health engineering,and environmental and food safety *** the encountered challenges and prospects are discussed,providing an outlook for the future development of aptamers.

关键词： Aptamer SELEX Engineering biology Biosensors

Analyzing hydro abrasive erosion in Kaplan turbine:A case study from India

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Journal of Hydrodynamics 2016年第5期28卷 863-872页

作者： Anant kr.RAI Arun KUMAR Alternate Hydro Energy Centre IIT Roorkee

Sediment flow through hydro turbine causes erosion of hydraulic components resulting in drop of turbine efficiency, parti- cularly in hydropower plants of the Himalayan region. The measurement of erosion and monitoring of sediment flow in turbine are major concerns in erosion study. Attempts have been made to study erosion mainly in Pelton and Francis turbines. In this study, a simple and effective method has been presented to measure erosion in a Kaplan turbine of a run-of-river scheme Chilla hydropower plant in foothills of Himalaya. Recent techniques were used to measure sediment parameters like concentration, size, shape and mineral content. A standard erosion model is applied to estimate the erosion in Kaplan turbine blade, runner chamber and draft tube cone. A calibration factor has been proposed to apply the erosion model for site specific conditions. It has been found that the outer trailing edges of the turbine blade and upper rtmner chamber are most erosion prone zones. Sediment analysis revealed that effective operation can reduce erosion in turbine components. The estimated erosion values from model are found to be consistent with measu- red values. Finally, suggestions for design improvements and effective operation of erosion affected hydropower plants are given.

关键词： turbine sediment erosion measurement IEC 62364 hydropower

Distribution of hepatitis B virus genotypes:Phylogenetic analysis and virological characteristics of Genotype C circulating among HBV carriers in Kolkata,Eastern India

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World Journal of Gastroenterology 2006年第37期12卷 5964-5971页

作者： arup Banerjee Sibnarayan Datta Partha K Chandra Susanta Roychowdhury Chinmoy Kumar Panda Runu Chakravarty ICMR Virus Unit Kolkata India Indian Institute of Chemical Biology Kolkata India Chittaranjan National Cancer Institute Kolkata India

AIM: To evaluate the genotype distribution of hepatitis B virus (HBV) in Eastern India and to clarify the phyloge- netic origin and virological characteristics of the recently identifi ed genotype C in this region. METHODS: Genotype determination, T1762/A1764 mutation in the basal core promoter (BCP) and A1896 mutation in the precore region of 230 subjects were de- termined by restriction fragment length polymorphism method (RFLP) and the result was confi rmed by direct sequencing. RESULTS: The predominant genotypes D (HBV/D) and A (HBV/A) were detected in 131/230 (57%) and 57/230 (25%) samples. In addition, genotype C (HBV/C) was detected in 42/230 (18%) isolates. Surface gene region was sequenced from 45 isolates (27 HBV/C, 9 HBV/A and 9 HBV/D). Phylogenetic analysis revealed that all of the HBV/C sequences clustered with South East Asian subgenotype (HBV/Cs). The sequence data showed re- markable similarity with a Thai strain (AF068756) (99.5% ± 0.4% nucleotide identities) in 90% of the genotype C strains analyzed. T1762/A1764 mutation in BCP re- gion, associated with high ALT was signifi cantly higher in HBeAg negative isolates than HBeAg positive isolates. Frequency of A1896 mutation leading to HBeAg negativ- ity was ***: The present study reports the genotypic distribution and the characteristics of partial genome sequences of HBV/C isolates from Eastern India. Low genetic diversity and confi nement of HBV/C in Eastern India possibly indicate a recent, limited, spread in this region. Genotype C with T1762/A1764 mutation has been reported to increase the risk for hepatocellular car- cinoma; therefore genotype C carriers in Eastern India should be carefully monitored.

关键词： HBV genotypes HBV/Cs, Eastern India T1762/A1764 mutation

Pre-treatment of rocks prior to comminution--A critical review of present practices

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International Journal of Mining Science and Technology 2017年第2期27卷 339-348页

作者： Somani Aditya nandi Tapas K. Pal Samir K. Majumder Arun K. Department of Mining Engineering Indian Institute of TechnologyKharagpur 721302India Cryogenic Engineering Centre Indian Institute of TechnologyKharagpur 721302India

It has been an established fact that comminution processes, crushing and grinding, are most energy intensive processes which account for more than half of the total energy consumed in mineral *** alternative pre-treatment methods have been tried by experts around the globe. Although these methods yielded positive results in terms of reduction in energy consumption in crushing and particularly, in grinding operations at laboratory scale, their industrial application still remains an unresolved issue and challenge. Present review paper describes each one of these methods along with outcome of earlier studies and issues that need to be addressed through further rigorous experimental *** also suggests the direction in which future studies can be carried out to meet the primary objective of making comminution processes more energy efficient than today they are.

关键词： Comminution Rock breakage Microwave Thermal shock

IRFs as competing pioneers in T-cell differentiation

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Cellular & Molecular Immunology 2017年第8期14卷 649-651页

作者： Andrea kröger Institute for Medical Microbiology Molecular MicrobiologyOtto-von-Guericke University of MagdeburgMagdeburg D-39120Germany

CD4-expressing T lymphocytes are among of the best-characterized immune system cells.1 They develop in the thymus and acquire the potential to differentiate into distinct *** leaving the thymus,T helper cells further ... 详细信息

关键词： lymphocytes expressing pioneer