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Transplanting embryonic stem cells onto damaged human corneal endothelium

World Journal of Stem Cells 2017年第8期9卷 127-132页

作者： Charles Hanson arsaell arnarsson Thorir Hardarson Ann Lindgard Mandana Daneshvarnaeini Catarina Ellerstrom Anita Bruun Ulf Stenevi Unit of Reproductive Medicine Sahlgrenska University Hospital Neuroscience Laboratory University of Akureyri Fertility Centre Scandinavia Carlanderska Hospital Department of Ophthalmology Gothenburg University Takara Bio Europe AB Arvid Wallgrens Backe 2041346 GothenburgSweden Department of Ophthalmology Lund University Hospital

AIM To investigate whether human embryonic stem cells(hESCs) could be made to attach, grow and differentiate on a human Descemet's membrane(DM).METHODS Spontaneously differentiated hESCs were transferred onto a human corneal button with the endothelial layer removed using ocular sticks. The cells were cultured on a DM for up to 15 d. The genetically engineered hESC line expressed green fluorescent protein, which facilitated identification during the culture experiments, tissue preparation, and analysis. To detect any differentiation into human corneal endothelial-like cells, we analysed the transplanted cells by immunohistochemistry using specific *** We found transplanted cells form a single layer of cells with a hexagonal shape in the periphery of the DM. The majority of the cells were negative for octamer-binding transcription factor 4 but positive for paired box 6 protein, sodium potassium adenosine triphosphatase(NaKATPase), and Zona Occludens protein 1. In four of the 18 trials, the transplanted cells were found to express CK3, which indicates that the stem cells differentiated into corneal epithelial cells in these cases. CONCLUSION It is possible to get cells originating from hESCs to become established on a human DM, where they grow and differentiate into corneal endothelial-like cells in vitro.

关键词： Embryonic stem cells Cornea Descemet’s membrane Endothelium Immunohistochemistry

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一项雷克雅未克成年人群5年屈光变化的眼研究

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世界核心医学期刊文摘（眼科学分册） 2005年第11期1卷 53-54页

作者： Gudmundsdottir E. arnarsson A. Jonasson F. 杨建刚 Department of Oph thalmology University of Iceland Landspitalinn 101 Reykjavik IcelandDr.

Purpose: To study refractive changes over the course of 5 years in citizens of Reykjavik 50 years and older at baseline. Methods: The cohort was a population -based random sample of citizens 50 years and older. Eight hundred forty-six of the 958 survivors (88.2%) underwent a 5-year follow-up examin ation. Refraction and keratometry were performed using a Nidek (Gamagori, Japan) ARK 900 autorefractor keratometer. Typing and grading of lens opacification was carried out using Scheimpflug slit-lamp and retroilluminated images (Nidek EAS 1000), and axial length was measured by Nidek Echoscan 800. Results: Seven hund red fifty-seven right eyes were available for refraction analysis. In the 50-t o 59-year age group and the 60-to 69-year age group at baseline, there was a hyperopic shift of 0.41 diopters (D) and 0.34D, respectively, during the 5 years . There was a 0.02-D myopic shift for those 70 years of age and older at baseli ne during the same period. Considering all right eyes, there was a hyperopic shi ft of +0.29 D over the 5 years, whereas eyes with nuclear lens opacification gr ade II or more at baseline had a mean myopic shift of-0.65 D. There was a mean change of 0.13 D in the astigmatism against the rule during the 5 years. The dat a on axial length are available from the followup study only. The mean axial len gth for those 50 to 59 years of age at baseline was 23.56mm (standard deviation [SD], 1.08 mm), as compared with 23.23 mm (SD, 1.27 mm) for those 70 years of ag e and older at baseline (P< 0.04). Conclusions: We found a hyperopic shift for t hose younger than 70 years of age at baseline, although eyes with nuclear opacif ication grade of II or more at baseline were associated with a myopic shift. The re was also an age-related shift against the rule for the axis of astigmatism d uring the 5-year period. Our results may be useful for predicting long-term ou tcome of refractive surgery.

关键词：雷克雅未克眼轴长度角膜曲率计检查逆规性散光晶状体核晶状体混浊轴长年龄组随访检查后照

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Reykjavik眼病研究中老年性黄斑病变的5年发病率

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世界核心医学期刊文摘（眼科学分册） 2005年第5期1卷 57-57页

作者： Jonasson F. arnarsson A. Peto T. 张少娟

To examine the age-and gender-specific 5-year incidence of age-related mac ulopathy (ARM) and age-related macular degeneration (AMD) in citizens of Reykja vik. Population-based, prospective cohort study. The cohort was a population-b ased random sample of citizens 50 years and older. Of 1379 eligible subjects, 10 45 had a baseline examination in 1996; 846 of the 958 survivors (88.2%) had a 5 -year follow-up examination in 2001. The incidence of various characteristics of drusen and pigmentary changes that are typical of ARM were determined using t he international classification and grading system for ARM and AMD. Early ARM an d AMD were assessed by masked grading of stereo fundus photographs. Hypopigmenta tion developed at 5 years in 10.7%of people 50 to 59 years of age (95%confiden ce interval [CI], 6.9-14.4) and in 25.7%those 70 to 79 years of age (95%CI, 1 8.4-33.0) at baseline. Age-related macular degeneration developed in no one wh o was 50 to 59 years of age at baseline. Geographic atrophy (GA) developed in 4. 6%(95%CI, 1.2-7.9) and exudative AMD in none of those who were 70 years and o lder at baseline. Geographic atrophy is the predominant type of AMD in Iceland, and the ratio of GA to neovascular AMD is higher than in racially similar popula tions.

关键词：黄斑病变 Reykjavik 老年性黄斑变性玻璃膜疣色素脱失随访调查国际分类前瞻性队列研究存活者色素改变

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