Transplanting embryonic stem cells onto damaged human corneal endothelium

作     者：Charles Hanson Arsaell Arnarsson Thorir Hardarson Ann Lindgard Mandana Daneshvarnaeini Catarina Ellerstrom Anita Bruun Ulf Stenevi 

作者机构：Unit of Reproductive MedicineSahlgrenska University Hospital Neuroscience LaboratoryUniversity of Akureyri Fertility Centre ScandinaviaCarlanderska Hospital Department of OphthalmologyGothenburg University Takara Bio Europe ABArvid Wallgrens Backe 2041346 GothenburgSweden Department of OphthalmologyLund University Hospital 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2017年第9卷第8期

页      面：127-132页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：De Blindas Vanner,Gothenburg,and Greta Bergs Foundation,Lerum(to Charles Hanson) University of Akureyri Research Fund the KEA Fund the Icelandic Council on Ageing(to Arsaell Arnarsson) Gothenburg Medical Society the Medical Faculty of the University of Gothenburg the Herman Svensson Foundation(to Ulf Stenevi) 

主　　题：Embryonic stem cells Cornea Descemet’s membrane Endothelium Immunohistochemistry 

摘      要：AIM To investigate whether human embryonic stem cells(hESCs) could be made to attach, grow and differentiate on a human Descemet s membrane(DM).METHODS Spontaneously differentiated hESCs were transferred onto a human corneal button with the endothelial layer removed using ocular sticks. The cells were cultured on a DM for up to 15 d. The genetically engineered hESC line expressed green fluorescent protein, which facilitated identification during the culture experiments, tissue preparation, and analysis. To detect any differentiation into human corneal endothelial-like cells, we analysed the transplanted cells by immunohistochemistry using specific *** We found transplanted cells form a single layer of cells with a hexagonal shape in the periphery of the DM. The majority of the cells were negative for octamer-binding transcription factor 4 but positive for paired box 6 protein, sodium potassium adenosine triphosphatase(NaKATPase), and Zona Occludens protein 1. In four of the 18 trials, the transplanted cells were found to express CK3, which indicates that the stem cells differentiated into corneal epithelial cells in these cases. CONCLUSION It is possible to get cells originating from hESCs to become established on a human DM, where they grow and differentiate into corneal endothelial-like cells in vitro.