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朝北依然有阳光

缤纷 2002年第8期 72-79页

作者： antonio ochoa 张霖郁周之毅

在北京居住的九年里,我总是生活在黯淡的光线里。家里的光线经常不足,透过小小的窗户,只能看见北京灰色和多云的天空,其实,在这样的环境里,即使你的公寓朝南,室内的光线也还会很暗。我们现在的家却布满阳光,恰好与中国的传统相反,房子... 详细信息

在北京居住的九年里,我总是生活在黯淡的光线里。家里的光线经常不足,透过小小的窗户,只能看见北京灰色和多云的天空,其实,在这样的环境里,即使你的公寓朝南,室内的光线也还会很暗。我们现在的家却布满阳光,恰好与中国的传统相反,房子的朝向是向北的,巨大的落地窗户可以捕捉到北京的每一缕优美光线,往往在清晨时就有的阳光穿过深色的木窗,温柔、平缓、安静地停栖在每一间屋子里。北边的屋子是给艺术家准备的,在那里可以获得最好的光线绘画;而在北京,不同于传统观念,朝北的屋子也同样生动美丽。

关键词：光线阳光艺术家传统观念委内瑞拉北京落地窗生活公寓房子

Plant cell wall-mediated disease resistance:Current understanding and future perspectives

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Molecular Plant 2024年第5期17卷 699-724页

作者： antonio Molina Lucıa Jorda Miguel Angel Torres Marina Martın-Dacal Diego Jose Berlanga Patricia Fernandez-Calvo Elena Gomez-Rubio Sonsoles Martın-Santamarıa Centro de Biotecnología y Genómica de Plantas Universidad Politécnica de Madrid(UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria(INIA/CSIC)Campus de Montegancedo UPMPozuelo de Alarcon(Madrid)Spain Departamento de Biotecnologıa-Biología Vegetal Escuela Técnica Superior de Ingenierıa AgronomicaAlimentarıa y de BiosistemasUPMMadridSpain Centro de Investigaciones Biologicas Margarita Salas Consejo Superior de Investigaciones Científicas(CSIC)Ramiro de Maeztu 928040 MadridSpain Misión Biológica de Galicia Consejo Superior de Investigaciones Científicas(CSIC)Campus de Montegancedo UPMPozuelo de Alarcón(Madrid)Spain

Beyond their function as structural barriers,plant cell walls are essential elements for the adaptation of plants to environmental *** walls are dynamic structures whose composition and integrity can be altered in response to environmental challenges and developmental *** wall changes are perceived by plant sensors/receptors to trigger adaptative responses during development and upon stress *** cell wall damage caused by pathogen infection,wounding,or other stresses leads to the release of wall molecules,such as carbohydrates(glycans),that function as damage-associated molecular patterns(DAMPs).DAMPs are perceived by the extracellular ectodomains(ECDs)of pattern recognition receptors(PRRs)to activate pattern-triggered immunity(PTI)and disease ***,glycans released from the walls and extracellular layers of microorganisms interacting with plants are recognized as microbe-associated molecular patterns(MAMPs)by specific ECD-PRRs triggering PTI *** number of oligosaccharides DAMPs/MAMPs identified that are perceived by plants has increased in recent ***,the structural mechanisms underlying glycan recognition by plant PRRs remain ***,this knowledge is mainly focused on receptors of the LysM-PRR family,which are involved in the perception of various molecules,such as chitooligosaccharides from fungi and lipo-chitooligosaccharides(i.e.,Nod/MYC factors from bacteria and mycorrhiza,respectively)that trigger differential physiological ***,additional families of plant PRRs have recently been implicated in oligosaccharide/polysaccharide *** include receptor kinases(RKs)with leucine-rich repeat and Malectin domains in their ECDs(LRR-MAL RKs),Catharanthus roseus RECEPTOR-LIKE KINASE 1-LIKE group(CrRLK1L)with Malectin-like domains in their ECDs,as well as wall-associated kinases,lectin-RKs,and *** characterization of structural basis of glycans recogniti

关键词： Arabidopsis thaliana cell wall damage-associated molecular patterns DAMPs disease resistance glycans oligosaccharides pattern-triggered immunity PTl pattern recognition receptor PRRs

Canola and Oat Forage Potential Evaluation in Four Early Planting Dates

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Phyton-International Journal of Experimental Botany 2019年第4期88卷 435-448页

作者： Juan Isidro Sánchez-Duarte David Guadalupe Reta-Sánchez Joséantonio Cueto-Wong Arturo Reyes-González Esmeralda ochoa-Martínez Campo Experimental La Laguna Instituto Nacional de Investigaciones ForestalesAgrícolas y Pecuarias(INIFAP).Blvd.JoséSantos Valdez 1200 Pte.Col.CentroMatamorosCoahuilaCP 27440México

Canola and oat forage potential may be affected by climatic conditions when sown *** objective of this study was to evaluate the forage canola and oat potential in four early sowing dates(September 11 and 25;October 9 and 23)during the 2012-2013 and 2013-2014 cycles in Matamoros,Coahuila,*** cycle duration,chemical composition,dry matter(DM),crude protein(CP),and net energy for lactation(NEL)yields were *** temperatures and long photoperiods affected crops seeded on September 11,accelerating growth and reducing canola(26.6%-31.7%)and oat(15.8%)DM *** of September 25,canola cv IMC 205 reached DM yields(7746 kg ha^(-1)-9276 kg ha^(-1))similar to those obtained by oat(8115 kg ha^(-1)-9507 kg ha^(-1)),while canola cv Hyola 401 obtained such yields only until October *** chemical composition was better than that found in oat,with higher CP,but lower acid detergent fiber(ADF)and neutral detergent fiber(NDF)*** equaled oat CP yields(972 kg ha^(-1)-1215 kg ha^(-1))in the first sowing date,while in the other three other canola sowings reached higher yields(1193 kg ha^(-1)-1889 kg ha^(-1)).As for NEL yields,no difference was observed between both *** best sowing date for canola is from September 25 on,with CP production advantages over oat.

关键词： Avena sativa L. Brassica napus L.var.oleifera chemical composition dry matter yield nutrients

Glial regenerative response in the imaginal discs of Drosophila melanogaster

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Neural Regeneration Research 2023年第1期18卷 109-110页

作者： Sergio B.Velarde antonio Baonza Centro de Biología Molecular“Severo ochoa” CSIC/UAMMadridSpain

Glial cells play a key role during nervous system development and actively participate in all the cellular processes involved in maintaining its structural robustness and functional *** response to neuronal damage,glial cells proliferate,migrate to the injured region and change their morphology,function,and behavior(Gallo and Deneen,2014;Kato et al.,2018).This glial regenerative response is associated with the repairing function of these cells and is found across species,suggesting that it may reflect a common underlying genetic mechanism(Kato et al.,2018).

关键词： damage migrate

First report of Phytopythium vexans causing the“Avocado sadness”in Michoacan,Mexico

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Phyton-International Journal of Experimental Botany 2019年第1期88卷 11-13页

作者： Hernandez Perez A E Cerna Chavez JC Delgado Ortiz M Beltran Beache LM Tapia Vargas YM ochoa Fuentes Estudiante del postgrado Universidad Autonoma Agraria antonio Narro Departamento de ParasitologiaBuenavistaSaltilloCoahuilaMexico CP 25315 Universidad Autonoma Agraria antonio Narro Departamento de ParasitologiaBuenavistaSaltilloCoahuilaMexico CP 25315 Catedratico CONACYT-UAAAN.Universidad Autonoma Agraria antonio Narro Departamento de ParasitologiaBuenavistaSaltilloCoahuilaMexico CP 25315 CULTA S.A.de C.V. InvestigacionDesarrolloAltavistaEl ManteTamaulipasMexico CP 898800 INIFAP Campo Experimental Uruapan.Av.Latinoamericana No.1101Col.Revolucion CP.60500UruapanMichoacanMexico

Mexico is the main producer,consumer and exporter of avocado in the world,being Michoacan the main producer state contributing more than 80%of the national *** are phytopathogens that decimate the production causing the death of the *** samples were collected in avocado trees that showed the characteristic symptomatology of the disease known as avocado sadness,the sampling was carried out in four of the main avocado producing towns,in the state of Michoacan,*** isolation consisted in sowing root tissue in Petri dishes with V8R-PARPH culture medium,subsequently they were identified morphologically and for species level it was determined by molecular biology,with the PCR-ITS *** tests were performed in triplicate with avocado seedlings with more than six *** 24 hours,the inoculated plants expressed decay in the apical part,after 120 hours the leaves showed yellowing and after 15 days there was a generalized wilt on the stem and leaves,re-isolating the phytopathogen Phytopythium *** study confirms the first report of the oomycete *** affecting avocado trees in the most important producing region of the Mexican Republic.

关键词： Molecular identification Pathogenicity Phytopythium vexans Avocado.

Non-Painful Peripheral Inflammation Blocks Conditioned Place-Preference to Morphine and Nicotine through an Ibuprofen-Sensitive and an Ibuprofen Insensitive Pathway

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Journal of Behavioral and Brain Science 2018年第2期8卷 57-76页

作者： Marco antonio Sotomayor-Sobrino Abraham ochoa-Aguilar Eda Patricia Tenorio Martha León-Olea Roberto Velasco-González Israel Luna-Mendoza Claudia Gómez-Acevedo Departmento de Farmacología Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México México Departmento de Bioquímica Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México México Instituto Nacional de Psiquiatría “Ramón de la Fuente Muñ iz” Ciudad de México México

The field of neuroimmunology has expanded in recent years providing new insights and therapies into pathologies like stroke, autism, and depression. However, few works explore the relationship between inflammatory stimuli and motivation. Thus, the aim of this study was to determine how non-painful inflammatory stimuli affect reward. To test reward-response, we used the morphine and the nicotine induced conditioned place-preference and place-aversion model in rats with non-painful inflammation. The following inflammatory models were used: non-painful infectious inflammation: 24 hrs prior to conditioning sessions, an injection with Calmette-Guerin bacillus (CGB) 1 × 107 cfu, ip, was administered. Non-painful non-infectious inflammation: 24 hrs prior to conditioning sessions, rats’ sciatic nerve was blocked and cut, followed by the injection of carrageenan (750 μl) in the paw. We then measured the cytokine concentration to determine the inflammatory profile of each of our models. Finally, we administered ibuprofen to determine if it could prevent the effect of inflammation over conditioned place-preference. We show that carrageenan significantly reduced the morphine-induced reward. Non-painful inflammatory stimulus, CGB and denervation + carrageenan, inhibit the conditioned place-preference to morphine and nicotine, CGB also block conditioned place-aversion to nicotine;carrageenan has no effect on CPA. The administration of ibuprofen reinstates conditioned place-preference to morphine and nicotine in the carrageenan model, but has no effect in the CGB model;finally ibuprofen has no effect on CPA. Our data suggest that non-painful-inflammatory stimuli inhibit the reward system, independent of cytokine concentration. Furthermore, the administration of a PGE 2 inhibitor can importantly modulate this phenomenon.

关键词： Reward Addiction Pain Inflammation Prostaglandins Interferon

SARS-CoV-2:Air pollution highly correlated to the increase in *** case of Guadalajara,Jalisco,Mexico

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Infectious Disease Modelling 2023年第2期8卷 445-457页

作者： Elizabeth Torres-Anguiano Itzel Sanchez-Lopez Angeles Garduno-Robles Jorge David Rivas-Carrillo Edgar Alfonso Rivera-Leon Sergio Sanchez-Enríquez Luis Fernando Ornelas-Hernandez Fernando Zazueta Leon-Quintero Eduardo Narciso Salazar Leon-Quintero Guillermo Enrique Juarez-Lopez Fernando antonio Sanchez-Zubieta Mariana ochoa-Bru Abraham Zepeda-Moreno Onkogenetik/Mexicana de Investigacion y Biotectogía S.A.de C.V GuadalajaraJaliscoMexico Unidad de Biología Molecular Investigacion y DiagnosticoHospital San JavierGuadalajaraJaliscoMexico Departamento de Fisiología Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajaraJaliscoMexico Departamento de Biología Molecular y Genomica Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajaraJaliscoMexico Departamento de Clínicas Centro Universitario de los AltosUniversidad de GuadalajaraTepatitlan de MorelosJaliscoMexico Departamento de Clínicas Medicas Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajaraJaliscoMexico Servicio de Hemato-Oncología Pediatrica Hospital Civil de Guadalajara“Dr.Juan I.Menchaca”GuadalajaraJaliscoMexico Hospital Civil de Guadalajara“Fray antonio Alcalde” GuadalajaraJaliscoMexico

Objectives:To determine whether air pollution or changes in SARS-CoV-2 lineages lead to an increase in ***:Descriptive statistics were used to calculate rates of infection(2020-2021).RT ePCR was used to compare viral loads from October 2020 to February *** sequencing(NGS)(n=92)was used to examine and phylogenetically map SARS-CoV-2 lineages.A correlative“air pollution/temperature”index(I)was developed using regression ***_(2.5),PM_(10),O_(3),NO_(2),SO_(2),and CO concentrations were analyzed and compared to the ***:The mortality rate during the last year was~32%.Relative SARS-CoV-2 viral loads increased in December 2020 and January *** revealed that approximately 80%of SARS-CoV-2 linages were B.1.243(33.7%),B1.1.222(11.2%),B.1.1(9%),B.1(7%),B.1.1.159(7%),and B.1.2(7%).Two periods were analyzed,the prehigh-and high-mortality periods and no significant lineage differences or new lineages were *** correlations of air pollution/temperature index values with mortality were found for IPM_(2.5) and IPM_(10).INO_(2).ISO_(2),and ICO but not for O_(3).Using ICO,we developed a model to predict mortality with an estimated variation of~±5 deaths per day.

关键词： Air pollution COVID-19 Guadalajara Mexico SARS-CoV-2 SARS-CoV-2 lineages

Geotechnical particle finite element method for modeling of soilstructure interaction under large deformation conditions

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Journal of Rock Mechanics and Geotechnical Engineering 2022年第3期14卷 967-983页

作者： Josep Maria Carbonell Lluís Monforte Matteo O.Ciantia Marcos Arroyo antonio Gens MECAMAT Group Department of EngineeringFaculty of Science and TechnologyUniversitat de Vic-Universitat Central de Catalunya(UVic-UCC)VicSpain Centre Internacional de Mètodes Numèrics a l'Enginyeria(CIMNE) BarcelonaSpain School of Science and Engineering University of DundeeDundeeUK Universitat Politècnica de Cataluna-BarcelonaTech BarcelonaSpain

The possibilities of the particle finite element method(PFEM)for modeling geotechnical problems are increasingly *** is a numerical approach to solve large displacement and large strain continuum problems that are beyond the capabilities of classical finite element method(FEM).In PFEM,the computational domain is reconfigured for optimal solution by frequent remeshing and boundary *** inherits many concepts,such as a Lagrangian description of continuum,from classic geomechanical *** familiarity with more popular numerical methods facilitates learning and *** work focuses on G-PFEM,a code specifically developed for the use of PFEM in geotechnical *** article has two *** first is to give the reader an overview of the capabilities and main features of the current version of the G-PFEM and the second is to illustrate some of the newer developments of the code.G-PFEM can solve coupled hydro-mechanical static and dynamic problems involving the interaction of solid and/or deformable *** constitutive models for geomaterials are available,including features,such as structure and destructuration,which result in brittle *** solutions are robust,solidly underpinned by numerical technology including mixedfield formulations,robust and mesh-independent integration of elastoplastic constitutive models and a rigorous and flexible treatment of contact *** novel features presented in this work include the contact domain technique,a natural way to capture contact interactions and impose contact constraints between different continuum bodies,as well as a new simplified formulation for dynamic impact *** code performance is showcased by the simulation of several soil-structure interaction problems selected to highlight the novel code features:a rigid footing insertion in soft rock,pipeline insertion and subsequent lateral displacement on over-consolidated clay,screw-pile pull-out and the dyn

关键词： Particle finite element method(PFEM) Structured soils Nonlocal elastoplasticity Contact domain method Soil penetration problems

Nanostructured biohybrid material with wide-ranging antiviral action

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Nano Research 2023年第8期16卷 11455-11463页

作者： Noelia Losada-Garcia Angela Vazquez-Calvo David Ortega-Alarcon Olga Abian Adrian Velazquez-Campoy Pilar Domingo-Calap antonio Alcami Jose M.Palomo Instituto de Catálisis y Petroleoquímica(ICP) CSICC/Marie Curie 2.Madrid 28049Spain Centro de Biología Molecular Severo ochoa Consejo Superior de Investigaciones Científicas(CSIC)-Universidad Autónoma de Madrid(UAM)Madrid 28049Spain Institute of Biocomputation and Physics of Complex Systems(BIFI) Joint Unit GBsC-CSIC-BIFIUniversity of ZaragozaZaragoza 50018Spain Fundación Instituto de Investigación Sanitaria de Aragón(IIS Aragón) Zaragoza 50009Spain CIBERehd Madrid 28029Spain Instituto de Biología Integrativa de Sistemas I2SyBioUniversitat de València-CSICValència E-46071Spain

Respiratory pathogens kill more people than any other infectious agent each year *** of novel,economically friendly,sustainable,and highly efficient materials against viruses is a major ***,we describe a nanostructured material composed of very small crystalline phosphate copper nanoparticles synthesized using a new biohybrid technology that employs a biological agent for its formation at room temperature in aqueous *** evaluation of different enzymes in the final preparation of the nanomaterial or even in synthetic methods was *** characterization revealed the formation of Cu species in the protein *** best biomaterial synthesized using a lipase called BioCuNPs showed excellent inhibition capacity against functional proteins of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2);for example,assent 3-chymotrypsin like protease(3CLpro)complete inhibition was achieved by using 5µg/mL,or acetone(ACE)–spike protein interaction was inhibited by more than 80%in the presence of 400µg/mL of *** these in vitro results into account,an efficacy analysis against human coronavirus 229E(HCoV-E229)coronavirus was performed.A virus reduction of 99%was obtained in 5 ***,SARS-CoV-2 virus was tested to demonstrate high efficiency,with>99%inhibition in 15 min using 500 microgram of *** determine the wide applicability of this nanohybrid against viruses,an evaluation was carried out against a non-enveloped virus such as Human Rhinovirus(HRV-14),obtaining a virus reduction of 99.9%in 5 ***,the virucidal capacity against different bacteriophages was also evaluated,obtaining an excellent inhibition effect against PhageΦX174(99.999%reduction in 5 min).

关键词： antiviral compound coronaviruses copper nanoparticles nanobiotechnology viruses