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Integrative Zoology 2015年第1期10卷 91-101页

作者： Yuwen CHUNG-DAVIDSON Huiyong WANG anne MSCOTT Weiming LI Department of Fisheries and Wildlife Michigan State UniversityEast LansingMIUSA

Mature male sea lampreys(Petromyzon marinus)release a sex pheromone,3-keto-petromyzonol sulfate(3kPZS),that induces sexually dimorphic behavioral responses in ***,the neural mechanism of such responses is mostly *** examined the neurotransmitter serotonin(5-hydroxytryptamine,5-HT)and the expression of 5-HT1A receptors in the forebrain and brainstem of sea lamprey exposed to the vehicle(0.91 ppm methanol)or 10−10 M 3kPZS for 2 h using high performance liquid chromatography-electrospray ionization tandem mass spectrometry,immunohistochemistry and real-time quantitative polymerase chain *** to 3kPZS for 2 h increased 5-HT concentration in the forebrain of adult females,whereas 5-HT was not detected in the forebrain of adult *** the contrary,3kPZS exposure decreased 5-HT concentration in the brainstem of adult females and had no effect in adult *** exposure evoked context-dependent sexual dimorphism in brain 5-HT1A receptor immunoreactivity,but had no effect on 5-HT1A mRNA concentrations in the brain with 2 h exposure *** appears that in sea lamprey pheromone 3kPZS affects the 5-HT system in the brain in a context-dependent,sexually dimorphic manner.

关键词： 5-HT1A receptor liquid chromatography-electrospray ionization tandem mass spectrometry motor output real-time quantitative polymerase chain reaction

Seasonal and habitat dependence of fleas parasitic on small mammals in Tanzania

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Integrative Zoology 2009年第2期4卷 196-212页

作者： anne LAUDISOIT Herwig LEIRS Rhodes MAKUNDI Boris Ronald KRASNOV Zoogeographical Research Unit Universitéde LiègeLiègeBelgium Evolutionary Ecology Group Universiteit AntwerpenAntwerpenBelgium General bacteriology Unit VARBrusselsBelgium Danish Pest Infestation Laboratory Department of Integrated Pest ManagementUniversity of AarhusKongens LyngbyDenmark Pest Management Centre Sokoine University of AgricultureMorogoroTanzania Mitrani Department of Desert Ecology Jacob Blaustein Institute for Desert ResearchBen-Gurion University of the NegevMidreshet Ben-GurionIsrael

We investigated host and flea species composition across different habitats during dry and rainy seasons in the Western Usambara Mountains in *** both seasons,similarity in flea species composition increased with an increase in the similarity in host species ***,between-season within-habitat as well as within-season between-habitat similarity in host species composition was higher than similarity in flea species *** of habitats according to their host and flea species composition demonstrated that the pattern of between-habitat similarity in both host and flea species composition varied *** the relatively rich mammal and flea fauna of the study region,the major contribution to variation in species composition between seasons and among habitats was due to a few species *** assemblages on Lophuromys kilonzoi Verheyen et al.,2007 and Praomys delectorum Thomas,1910 in different habitats were equally similar in either *** contrast,flea assemblages on Mastomys natalensis(Smith,1834)occurring in different habitats were more similar in the dry than in the rainy season,whereas the opposite was the case for fleas on Grammomys *** different hosts,the main differences in species composition of flea assemblages between seasons as well as among habitats were due to different flea *** our results support the earlier idea that parasite species composition is determined by both host species composition and habitat properties,the former appears to explain variance in flea species composition between localities in the tropics better than between localities in temperate and arid zones.

关键词： Africa flea assemblages rodents seasonal tropical habitats.

A Novel Two Domain-Fusion Protein in Cyanobacteria with Similarity to the CAB/ELIP/HLIP Superfamily： Evolutionary Implications and Regulation

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Molecular Plant 2008年第1期1卷 155-166页

作者： Oliver Kilian anne Soisig Steunou Arthur R. Grossman Devaki Bhaya Department of Plant Biology The Carnegie Institution Stanford CA 94305 USA

Vascular plants contain abundant, light-harvesting complexes in the thylakoid membrane that are non-covalently associated with chlorophylls and carotenoids. These light-harvesting chlorophyll a/b binding （LHC） proteins are members of an extended CAB/ELIP/HLIP superfamily of distantly related polypeptides, which have between one and four transmembrane helices （TMH）. This superfamUy includes the single TMH, high-light-inducible proteins （Hlips）, found in cyanobacteria that are induced by various stress conditions, including high light, and are considered ancestral to the LHC proteins. The roles of, and evolutionary relationships between, these superfamily members are of particular interest, since they function in both light harvesting and photoprotection and may have evolved through tandem gene duplication and fusion events. We have investigated the Hlips （hli gene family） in the thermophilic unicellular cyanobacterium Synechococcus OS-B＇. The five hli genes present on the genome of Synechococcus OS-B＂ are relatively similar, but transcript analyses indicate that there are different patterns of transcript accumulation when the cells are exposed to various growth conditions, suggesting that different Hlips may have specific functions. Hlip5 has an additional TMH at the N-terminus as a result of a novel fusion event. This additional TMH is very similar to a conserved hypothetical, single membrane-spanning polypeptide present in most cyanobacteria. The evolutionary significance of these results is discussed.

关键词： fusion proteins Cyanobacteria N-Terminus stress conditions Large Hadron Collider Growth conditions photostability Regulation (biology) Thylakoid Membrane Tracheobionta gene duplication Analogy superfamily Chlorophyll Evolutionary

Laboratory and field testing of bolting systems subjected to highly corrosive environments

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International Journal of Mining Science and Technology 2017年第1期27卷 101-106页

作者： Meikle Tom Tadolini Stephen C. Sainsbury Bre-anne Bolton John Minova Richmond Vale NSW 2327Australia Minova Georgetown KY 43204USA Mining and Resource Engineering Monash University VIC 3800 CaulfieldAustralia

The capacity of ground support components which have been affected by corrosion is reduced and may ultimately lead to dynamic failure of the component and the strata. In order to maintain an effective,long-term ground support system, significant campaigns of rehabilitation are often required in corrosion affected areas which also expose the workers to hazardous conditions. The most common corrosion protection for steel ground support utilises sacrificial systems such as galvanising. Galvanising has previously been proven to be susceptible to some corrosion processes. Stainless steel is the most effective in resistance to corrosion, but can be cost prohibitive, and its mechanical properties often make it unsuited to use in ground support components. Providing an outer protective plastic coating to bolts has proven to be an effective means of protecting the inner steel bar from corrosion. However, these support systems tend to be susceptible to coating damage, and require post cement grouting to provide full encapsulation. In comparison to a standard bolt/resin system, they can be slow to install and *** systems have also been shown to reduce overall load transfer performance of the bolting system. In order to provide a higher level of corrosion protection whilst maintaining current installation practices and bolting cycle times, Minova has developed the Enduro^(TM)steel ground support range. The Enduro^(TM) range consists of standard Minova steel ground support components which have been treated with a unique coating process. The Enduro^(TM)coating has been tested in the harshest of conditions, in laboratory controlled conditions and in underground trials. It has been proven to effectively resist or completely eliminate the formation of corrosion, even in the most aggressive environments. This paper explains the process and provides the details of the laboratory and underground corrosion performance testing carried out on Enduro^(TM)ground support product

关键词： Corrosion Coatings Galvanised Salt spray Accelerated corrosion testing

In vitro immunotoxicity of untreated and treated urbanwastewaters using various treatment processes to rainbow trout leucocytes

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Journal of Environmental Sciences 2013年第7期25卷 1400-1407页

作者： Franois Gagné Marlène Fortier Michel Fournier Shirley-anne Smyth Emerging Methods Aquatic Contaminants Research Division Water Science and Technology Environment Canada105 McGill Street Montral Qubec Canada INRS-Institut Armand-Frappier 531 boul.des Prairies Laval Qubec Canada Effluents and Wastewaters Aquatic Contaminants Research Division Water Science and Technology Environment Canada867 Lakeshore Road BurlingtonOntario Canada

Municipal effluents are known to impede the immune system of aquatic organisms. The purpose of this studywas to examine the immunotoxicity of urbanwastewaters before and after 6 treatment processes from 12 cities toward trout leucocytes. Freshly prepared trout leucocyteswere exposed to increasing concentrations of solid phase （C18） extracts ofwastewaters for24 hr at 15°C. Immunocompetencewasdetermined by following changes in leucocyte viability and the proportion of cells able to ingest at least one （immunoactivity） and at least three （immunoefficiency） fluorescent beads. The influentswere treated by sixdifferent treatment strategies consisting of facultative aerated lagoons, activated sludge, biological aerated filter, biological nutrient removal, chemically-assisted physical treatment and trickling filter/solid contact. Water quality parameters of thewastewaters revealed that the plants effectively removed total suspended solids and reduced the chemical oxygendemand. The results revealed that the effluents＇ immunotoxic propertieswere generally more influenced by the properties of the untreatedwastewaters than by the treatment processes. About half of the incoming influentsdecreased leucocyte viabilitywhile4 treatment plantswere able to reduce toxicity. The influents readily increased phagocytosis activity for 8/12 influentswhile itwasdecreased in4/12 influents. This increasewas abolished for4/12 of the effluents using treatments involving biological and oxidative processes. In conclusion, municipal effluents have the potential to alter the immune system in fish and more researchwill be needed to improve the treatments ofwastewaters to better protect the quality of the aquatic environment.

关键词： municipal effluents fish leucocytes viability phagocytosis

Persistent organic pollutants and male reproductive health

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Asian Journal of Andrology 2014年第1期16卷 71-80页

作者： anne Vested Aleksander Giwercman Jens Peter Bonde Gunnar Toil- Department of Occupational Medicine Danish Ramazzini Centre Aarhus University Hospital Aarhus Denmark Reproductive Medicine Centre Lund University Skane University Hospital Malm6 Sweden Department of Occupational and Environmental Medicine Bispebjerg Hospital of Copenhagen University

Environmental contaminants such as persistent organic pollutants （POPs） are man-made bioaccumulative compounds with long half-lives that are found throughout the world as a result of heavy use in a variety of consumer products during the twentieth century. Wildlife and animal studies have long suggested adverse effects of exposure to these compounds on human reproductive health, which, according to the endocrine disrupter hypothesis, are ascribed to the compounds＇ potential to interfere with endocrine signaling, especially when exposure occurs during certain phases of fetal and childhood development. An extensive number of epidemiological studies have addressed the possible effects of exposure to POPs on male reproductive health, but the results are conflicting. Thus far, most studies have focused on investigating exposure and the different reproductive health outcomes during adulthood. Some studies have addressed the potential harmful effects of fetal exposure with respect to malformations at birth and/ or reproductive development, whereas only a few studies have been able to evaluate whether intrauterine exposure to POPs has long-term consequences for male reproductive health with measurable effects on semen quality markers and reproductive hormone levels in adulthood. Humans are not exposed to a single compound at a time, but rather, to a variety of different substances with potential divergent hormonal effects. Hence, how to best analyze epidemiological data on combined exposures remains a significant challenge. This review on POPs will focus on current knowledge regarding the potential effects of exposure to POPs during fetal and childhood life and during adulthood on male reproductive health, including a critical revision of the endocrine disruption hypothesis, a comment on pubertal development as part of reproductive development and a comment on how to account for combined exposures in epidemiological research.

关键词： endocrine disruption male reproduction persistent organic compounds reproductive hormones semen quality

Trends and seasonality in hospitalizations for acute alcohol-related and biliary pancreatitis in the USA

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Hepatobiliary & Pancreatic Diseases International 2021年第2期20卷 173-181页

作者： Artem Shmelev anne M Sill Tiffany Horrigan Steven C Cunningham Department of Surgery Saint Agnes HealthcareBaltimoreMDUSA

Background:The incidence of acute pancreatitis(AP)is characterized by circannual and geographical *** aim of this study was to describe seasonal variation and trends in hospitalizations for AP in the USA with respect to AP ***:The Nationwide Inpatient Sample data(2000–2016)from the Healthcare Cost and Utilization Project were *** study population included all primary hospitalizations for *** AP(BAP)and alcohol-induced AP(AAP)were distinguished by diagnostic and procedural ICD *** trend decomposition was ***:There was a linear increase in annual incidence(per 100000 population)of AAP in the USA(from 17.0 in 2000 to 22.9 in 2016),while incidence of BAP,equaled 19.9 in 2000,peaked at 22.1 in 2006 and decreased to 17.4 in *** incidence demonstrated 18%annual incidence amplitude with summer peak and winter trough,more prominent in *** 2016,within AAP,the highest incidence(per 100000 population)was noted among African-Americans(up to 50.4),followed by males aged 56–70 years(26.5)and Asians of low income(25.5);within BAP,above the average incidence was observed in Hispanic(up to 25.8)and Asian(up to 25.0)*** most consistent and rapid increase in AP incidence was noted in males aged 56–70 years with an alcoholic etiology(average 6%annual incidence growth).Conclusions:The incidence and annual trends of AP vary significantly among demographic and socioeconomic groups and this knowledge may be useful for the planning of healthcare resources and identification of at-risk populations.

关键词： Pancreatitis Alcoholic pancreatitis Incidence Seasonal variation

Corneal neurotization: a narrative review of techniques, outcomes, and surgical considerations

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Annals of Eye Science 2023年第2期8卷 1-13页

作者： Jessica Zhang anne Barmettler Department of Ophthalmology and Visual Sciences Montefiore Medical CenterAlbert Einstein College of MedicineBronxNew YorkUSA

Background and Objective:Corneal neurotization is a novel surgical technique used to restore corneal sensation in patients with neurotrophic *** keratopathy is a disorder characterized by dysfunction of the ophthalmic division of the trigeminal nerve,which provides sensory innervation to the *** sensation,the cornea is at risk of infection,ulceration,perforation,and ultimately,vision *** neurotization has emerged as an innovative technique to reinnervate anesthetized corneas by transferring a healthy donor nerve to the affected eye around the corneoscleral *** the field of corneal neurotization rapidly grows,there is a need to synthesize the existing body of literature on corneal neurotization and identify important areas for further *** this review,we will discuss neurotrophic keratopathy and its current management strategies,followed by an overview of corneal neurotization techniques,outcomes,surgical considerations,and future ***:PubMed and Google Scholar searches were conducted to retrieve and analyze relevant original papers and reviews on neurotrophic keratopathy and corneal neurotization up until April *** Content and Findings:Currently,numerous techniques for corneal neurotization exist,including direct nerve transfers,as well as indirect neurotization via interposition nerve *** far,corneal neurotization has been shown to be highly successful in restoring corneal sensation,improving visual acuity,and improving corneal epithelial *** date,there have been no significant differences in outcomes between direct versus indirect neurotization techniques,different donor nerves,or autologous versus allogeneic interposition ***,there is some evidence that corneal neurotization procedures may be more successful in pediatric ***:Corneal neurotization shows great promise in treating neurotrophic corneas and represents the first management option to date tha

关键词： Corneal neurotization neurotrophic keratopathy corneal sensation neurotrophic cornea

Microstructure and texture evolution of nonoriented silicon steel during the punching process

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International Journal of Minerals,Metallurgy and Materials 2022年第11期29卷 2064-2071页

作者： Xiang Fang Wei Wang François Brisset anne-Laure Helbert Thierry Baudin School of Automotive Engineering Wuhan University of TechnologyWuhan 430070China Hubei Key Laboratory of Advanced Technology for Automotive Components Wuhan University of TechnologyWuhan 430070China Hubei Collaborative Innovation Center for Automotive Components Technology Wuhan University of TechnologyWuhan 430070China Hubei Research Center for New Energy&Intelligent Connected Vehicle Wuhan University of TechnologyWuhan 430070China Institut de Chimie Moléculaire et des Matériaux d’Orsay UniversitéParis-SaclayCNRS91405 OrsayFrance

The iron core of a motor is mainly manufactured from rolled nonoriented silicon steel using a punching process that leads to deformation and texture evolution at the cutting *** to this process,circular samples of nonoriented silicon steel were prepared by punching using blunt punch *** this work,two positions along the rolling and transverse directions at the cutting edge were *** main mechanisms of deformation for both positions are dislocation slip and formation of shear *** two mechanisms lead to similar texture evolutions for both *** dislocation slip leads to the formation of the{221}component in the unbending area(200μm away from the cutting edge)and intermediate continuum-bent ***,the evolution of the texture from the{111}γfiber to the{110}fiber was observed at the extremity of the cutting edge with the formation of shear bands.

关键词： microstructure texture nonoriented silicon steel punching

Designing soft/hard double network hydrogel microsphere/UHMWPE composites to promote water lubrication performance

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Friction 2021年第3期9卷 551-568页

作者： Chaobao WANG Xiuqin BAI Conglin DONG Zhiwei GUO Chengqing YUAN anne NEVILLE School of Energy and Power Engineering Wuhan University of TechnologyWuhan 430063China Key Laboratory of Marine Power Engineering&Technology(Ministry of Transport) Wuhan University of TechnologyWuhan 430063China Institute of Functional Surfaces School of Mechanical EngineeringUniversity of LeedsLeeds LS29JTUnited Kingdom

Several soft tissues residing in the living body have excellent hydration lubrication properties and can provide effective protection during relative *** order to apply this advantage of soft matters in practical applications and try to avoid its disadvantage,such as swelling and weakening in water,a design strategy of a soft/hard double network(DN)hydrogel microsphere modified ultrahigh molecular weight polyethylene(UHMWPE)composite is proposed in this study.A series of microspheres of urea-formaldehyde(UF),polyacrylamide(PAAm)hydrogel,UF/PAAm double network,and their composites were *** mechanical properties,swelling,wettability,friction properties,and the lubrication mechanisms of the composites were *** results show that DN microspheres can have an excellent stability and provide hydration *** performance of 75 DN-1 composite was superior to *** finding will provide a novel strategy for the development of water-lubricated materials and have wide application in engineering fields.

关键词： double network microsphere composite friction properties hydration lubrication