检索结果-南通市图书馆

Mutations in Spike Gene of SARS-CoV-2 that Are Associated with a Higher Viral Load: A Clinical Case Study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Case Reports in Clinical Medicine 2022年第11期11卷 474-486页

作者： Ibrahim Keita Garan Dabo Lassina Doumbia Youssouf Diarra Ibrahim Traoré Mariam Traoré Lansana Sangaré Yacouba Toloba Mahamadou Alpha Diallo Djeneba Sy Andres H. Gutierrez Aliou Sissako Ibrahim Guindo Mohamed Diallo Akory Ag Iknane anne S. De Groot Ousmane Aliou Koita Laboratoire de Biologie Moléculaire Appliquée Université des Sciences Techniques et des Technologies de Bamako Bamako Mali Hô pital du Mali Ministère de la Santé Publique et du Développement Social Bamako Mali Centre Hospitalo-Universitaire du Point G Ministère de la Santé Publique et du Développement Social Bamako Mali epiVax Inc. Providence USA Institut National de Santé Publique Ministère de la Santé Publique et du Développement Social Bamako Mali Center for Vaccines and Immunology College of Veterinary Medicine University of Georgia Athens USA

Background: Quantitative PCR (qPCR) can be used to detect and quantify a load of a pathogen. It is a good indicator of the degree of transmissibility. While performing routine qPCR, we observed an unusually short cycle threshold (Ct) value of SARS-CoV-2 for a clinical specimen obtained in Bamako, Mali. This prompted us to sequence the short-cycle SARS-CoV-2 sample to identify potential mutations in the Spike gene (S gene) gene. Methods: Post-infection, Quantitative Reverse Transcription (qRT-PCR) was performed over a defined time course to estimate the Ct of the SARS-CoV-2 specimen collected from the patient. Sanger sequencing was done on the entire fragment of the S gene to identify mutations. Findings: Sanger sequencing revealed mutations in the lineage of interest, designated B.1.525 by Pango, and also known as “eta” using the nomenclature defined by WHO. This variant was originally found in Nigeria and Italy. The four novel mutations identified in eta (D228N, Y451N, I1172M, and C1250F) were otherwise observed with a low frequency worldwide. Although the initial Ct was 10 in the case study patient, he did not exhibit severe symptoms of SARS-CoV-2, for example, pneumonia. However, we observed a longer viral clearance period than usual, of 3 weeks. We note that as compared to SARS-CoV-2 samples obtained during the first peaks of SARS-CoV-2 infection in Mali, when the infection was at its peak in March 2020 (Ct = 30.4), circulating strains evaluated at the time the eta sample was obtained demonstrated a lower mean Ct (Ct = 24). Conclusions: The short cycle threshold associated with this variant, and the temporal association with a decrease in the mean Ct in the region of Bamako, may indicate higher levels of transmissibility due to a circulating variant. This variant is a lineage of interest designated B.1.525 by Pango or eta by WHO.

关键词： SARS-CoV-2 Variant eta Ct Value Mali

*** on central *** vision for visual search in younger and older adults

维普期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Annals of eye Science 2019年第1期 186-186页

作者： anne-Sophie Laurin Julie Ouerfelli-Éthier Laure Pisella Aarlenne Khan Department of Psychology University of MontrealMontrealQCCanada School of Optometry University of MontrealMontrealQCCanada ImpAct INSERM UM1028CNRS UMR 5292BronFrance

Background:It has been suggested that older adults show a reduced attentional field compared to younger *** may be attributed to a poorer utilization of peripheral vision(i.e.,peripheral attentional allocation)and a higher reliance on central vision compared to younger *** test this,we examined the importance of central,peri-foveal and near periphery information in younger and older adults by comparing their visual search performance while their central vision was blocked,in the presence of different sized artificial central *** tested participants in two versions of visual search,pop-out and serial search,because they require a different use of central and peripheral ***-out search relies on processing of the entire visual scene(i.e.,global processing)whereas serial search requires processing of each feature serially(i.e.,local processing).Methods:Thirteen healthy younger(M=21.8,SD=1.5)and 15 older adults(M=69.1 years,SD=7.3)performed a pop-out and a serial version of a visual search task in the presence of different sized gaze-contingent artificial central scotomas(no scotoma,3°diameter,5°and 7°).Participants were asked to indicate as quickly as possible whether a target was present or not among distractors whose number varied(16,32 or 64 objects).Results:We found evidence for a greater decline in peripheral processing in older adults compared to younger in pop-out but not in serial *** the pop-out condition with no scotoma,we found that the further the target in the periphery,the longer the search time,and that this increase was proportionally greater for older adults compared to younger ***,increases in scotoma size were associated with a greater increase in reaction times for older adults compared to younger *** the serial condition,both groups showed similar increases in reaction times with target distance from center and scotoma *** surmise that this may be due to task difficulty in serial sea

关键词： Visual search spatial attention peripheral central aging

earthworm functional groups are related to denitrifier activity in riparian soils

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2021年第3期31卷 413-422页

作者： Chen CHeN Leanne eJACK Martin RCHÉNIeR Joann KWHALeN State environmental Protection Key Laboratory of Urban ecological environment Simulation and Protection South China Institute of Environmental SciencesMinistry of Ecology and Environment of ChinaGuangzhou 510535China Department of Natural Resource Sciences Macdonald CampusMcGill University21111 Lakeshore RoadSte-Anne-de-BellevueQuebec H9X 3V9Canada Department of Food Science and Agricultural Chemistry and Department of Animal Science Macdonald CampusMcGill University21111 Lakeshore RoadSte-Anne-de-BellevueQuebec H9X 3V9Canada

Riparian buffers,located in the transition zone between terrestrial and aquatic ecosystems,are a hotspot for nitrogen(N)removal through *** are abundant in riparian buffers and may enhance *** study investigated earthworm demographics of three earthworm functional groups(anecic,epigeic,and endogeic)and denitrifier activity in temporarily flooded and non-flooded riparian soils from April to October 2012 in southern Quebec,*** earthworm species,mostly endogeic,were found in the temporarily flooded soil,while only six earthworm species were found in the non-flooded *** average,there were 11.7 times more earthworms with 12.4 times greater biomass(Pe temporarily flooded soil than in the non-flooded *** denitrification enzyme activity(DeA)was of similar magnitude in temporarily flooded and non-flooded soils,with temporal variation associated with rainfall *** earthworm biomass was positively correlated(PeA,while epigeic earthworm biomass was positively correlated(Pene copies and nosZ gene copies from bacteria,indicating an association between earthworm functional groups and denitrifier activity in riparian *** multiple regressions showed that DeA in riparian soils could be predicted using soil moisture,inorganic N concentration,and earthworm functional groups,suggesting that endogeic and epigeic earthworms contributed to denitrifier activity in riparian soils.

关键词： endogeic earthwormsepigeic earthwormsriparian areasriparian buffermacrofaunaseasonal changedenitrification enzyme activitydenitrifier gene copy

Does Dust from Arctic Mines Affect Caribou Forage?

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of environmental Protection 2017年第3期8卷 258-276页

作者： Wenjun Chen Sylvain G. Leblanc H. Peter White Christian Prevost Brian Milakovic Christine Rock Greg Sharam Harry O’Keefe Laura Corey Bruno Croft anne Gunn Sjoerd van der Wielen Adeline Football Boyan Tracz Jody Snortland Pellissey John Boulanger Canada Centre for Remote Sensing Natural Resources Canada Ottawa Canada eRM environmental Ressources Management Vancouver Canada Dominion Diamond ekati Corporation Yellowknife Canada environment and Natural Resources GNWT Yellowknife Canada Circum Arctic Rangifer Monitoring and Assessment Network Salt Spring Island Canada Tlicho Government Behchoko NT Canada Wek’èezhìi Renewable Resources Board Yellowknife Canada Integrated ecological Research Ltd. Nelson Canada

This study explores how dust from the ekati Diamond Mine potentially affects the availability and quality of forage on the seasonal range of the Bathurst caribou herd. Understanding the effects of dust as a source of disturbance is important because the Bathurst caribou population has declined by 93% since the middle 1980s and there are reports that caribou in general may avoid mining projects. There are several challenges for quantifying dust impacts: 1) Natural variations (e.g., topography, natural disturbance, and soil pH) may also impact forage availability and quality for caribou. To minimize their masking effect, we stratified survey sites into seven land cover classes and selected the most populous class (i.e., the dwarf shrub) for assessing the impact. 2) Within class variation (e.g., the proportion of area covered by rocks where vascular plants and lichen do not grow) can further skew the analysis. We eliminated this problem by examining only the area not covered by rocks. 3) Coarse and fine suspended particulates have different spatial coverages, chemical compositions, and pH values. Consequently, their impacts on caribou forage can be different. To distinguish their impacts, we sampled two areas: transects from the Misery Haul Road that has been in active use vs. those from a rarely used spur road outside the Misery Camp. We sampled percent vegetation cover, soil pH, and dust on leaves along these transects during the summers of 2015 and 2016. Our results indicated that the amount of dust on leaves in a zone of ~1000 m from the Misery Haul Road was 3 - 9 times than that of background sites. The zone of reduced lichen percent cover was also about 1000 m. In contrast, these road dust-induced changes in caribou forage were not observed for the dust-free transect from the spur road.

关键词： Mining Arctic Caribou Habitat Road Dust PM2.5 Vegetation Cover Lichen Soil pH Dust Deposition

Is there a way out for the 2014 ebola outbreak in Western Africa?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2015年第10期8卷 757-762页

作者： Angelo Andreas Christian Binoun A egom Peter Kruzliak emmanuel e.egom University of Toronto Scarborough Campus University of Ndjamena Faculty of Medicine International Clinical Research Center St.Anne's University Hospital and Masaryk University egom Clinical and Translational Research Services

The 2014 ebola outbreak in West Africa,primarily affecting *** Leone,and Liberia,has exceeded all previous ebola outbreaks in the number of cases and in international *** infections only occur frequently in Western Africa,the virus has the potential to spread globally and is classified as a category A pathogen that could be misused as a bioterrorism *** review aims(i) to discuss the latest data to aid our current recommendations for the prevention and control of the ebola vims infection,(ii) to review its pathophysiology as well as offering insights on the most current data available about ebola vaccine progress and potential use.

关键词： ebola virus Infection Prevention Control

学生公寓,埃因霍芬理工大学,埃因霍芬,荷兰

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

世界建筑 2018年第11期 66-71页

作者：吕德.布劳韦斯王单单鹿特丹及马斯特里赫特城市规划和遗产委员会

埃冈霍芬理工大学沿河岸的多梅尔山谷延伸开来。1950年代由城市规划师S．J．凡·艾本恩创作的设计图，如今被用作指导方针。现代风格的大楼被置于一片松散的布局中，如同将零碎摆件放置于正在进行中的景观里。为了解决对于学生公寓及社... 详细信息

埃冈霍芬理工大学沿河岸的多梅尔山谷延伸开来。1950年代由城市规划师S．J．凡·艾本恩创作的设计图，如今被用作指导方针。现代风格的大楼被置于一片松散的布局中，如同将零碎摆件放置于正在进行中的景观里。为了解决对于学生公寓及社会住宅的紧急需求，Stichting Woonbedrijf SWS／Hhvl公司主动为300名学生设计了一个住宅小区。

关键词：学生公寓大学荷兰城市规划师现代风格社会住宅住宅小区设计图

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

2008我们的地球

明日风尚 2008年第5期 33-80页

作者： Josephine Shen A-Link Agnès Sinai anne Rialhe Olivier Bailly Sabine RabOurdin Dominioue Vidal Donatien Garnier Alain Zecchini Marc Laimé Rémi Parmentier Michel Urvoy Dorothée Benoit-Browaeys Dominique Vidal Laurent Tubiana Nadège Figarol Thierry Salomono Philippe Bovet Claude Aubert 不详 Institutd′études politiques de Paris ecole des mines de Paris 《法国世界外交论衡》 Collectif Argos协会国际环境顾问公司Varda集团《Ouest-France》日报 VivAgora协会可持续发展及国际关系学院负瓦特协会

2008年,这一刻,我们的地球怎样了?《明日风尚》在内地华文媒体之中,首先取得《法国世界外交论衡月刊》(Le Monde Diplomatique)特别授权,辑录了该国际性月刊今年推出的"世界地图集",以大量地图和图表,向华文读者展现我们地球的最新状况... 详细信息

2008年,这一刻,我们的地球怎样了?《明日风尚》在内地华文媒体之中,首先取得《法国世界外交论衡月刊》(Le Monde Diplomatique)特别授权,辑录了该国际性月刊今年推出的"世界地图集",以大量地图和图表,向华文读者展现我们地球的最新状况。地球明显盛载着战争、疾病、贫穷、疯狂和环境破坏。我们从"天视"的角度去俯瞰地球,不出我们所料,地球形势叫人不安。深海水产遭到可以装下整座巴黎铁塔的渔网的无情扫荡。森林采伐、疯狂贸易,进一步导致地球气温攀升。水源匮乏进入了五十年倒数的灾难阶段。紧接着令人忧心忡忡的基因改造生物技术,这年头,我们又迎来了"微不足道"同时危险莫测的"纳米科技"。文明国家可以联合起来做些什么吗?对不起,我们发现国际上普遍存在企业环保责任的"司法空白"。跨国企业一方面牟取暴利肆意破坏环境,一方面展开"伪绿色宣传",为企业形象"整容"。原来"国有国法,家有家规"之外,我们并没有一条"地球法"。"生物多样性"正呈现明显衰退,远古出现过五次大规模生物灭绝,现在我们是否已踏进第六波生物灭绝?下一页,你将从"地球人视角"看到宏观的世界。——编者——

关键词：生物灭绝环境破坏明日风尚生物多样性京都议定书世界地图集森林采伐消费生活环境问题华文

Orally administered extract from Prunella vulgaris attenuates spontaneous colitis in mdr1a^(-/-) mice

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Pharmacology and Therapeutics 2015年第4期6卷 223-237页

作者： Kelley MK Haarberg Meghan J Wymore Brand anne-Marie C Overstreet Catherine C Hauck Patricia A Murphy Jesse M Hostetter Amanda e Ramer-Tait Michael J Wannemuehler Department of Veterinary Microbiology and Preventive Medicine College of Veterinary Medicine Iowa State University Department of Food Science and Human Nutrition Iowa State University Department of Veterinary Pathology College of Veterinary Medicine Iowa State University Department of Food Science and Technology University of Nebraska-Lincoln

AIM: To investigate the ability of a Prunella vulgaris(P. vulgaris) ethanolic extract to attenuate spontaneous typhlocolitis in mdr1a-/- mice. MeTHODS: Vehicle(5% ethanol) or P. vulgaris ethanolic extract(2.4 mg/d) were administered daily by oral gavage to mdr1a-/- or wild type FVBWT mice from 6 wk of age up to 20 wk of age. Clinical signs of disease were noted by monitoring weight loss. Mice experiencingweight loss in excess of 15% were removed from the study. At the time mice were removed from the study, blood and colon tissue were collected for analyses that included histological evaluation of lesions, inflammatory cytokine levels, and myeloperoxidase activity. ReSULTS: Administration of P. vulgaris extracts to mdr1a-/- mice delayed onset of colitis and reduced severity of mucosal inflammation when compared to vehicle-treated mdr1a-/- mice. Oral administration of the P. vulgaris extract resulted in reduced(P erum levels of IL-10(4.6 ± 2 vs 19.4 ± 4), CXCL9(1319.0 ± 277 vs 3901.0 ± 858), and TNFα(9.9 ± 3 vs 14.8 ± 1) as well as reduced gene expression by more than two-fold for Ccl2, Ccl20, Cxcl1, Cxcl9, IL-1 α, Mmp10, VCAM-1, ICAM, IL-2, and TNFα in the colonic mucosa of mdr1a-/- mice compared to vehicle-treated mdr1a-/-mice. Histologically, several microscopic parameters were reduced(P eated mdr1a-/-mice, as was myeloperoxidase activity in the colon(2.49 ± 0.16 vs 3.36 ± 0.06, P e numbers of CD4+ T cells(2031.9 ± 412.1 vs 5054.5 ± 809.5) and germinal center B cells(2749.6 ± 473.7 vs 4934.0 ± 645.9) observed in the cecal tonsils of P. vulgaris-treated mdr1a-/- were significantly reduced(P ehicle-treated mdr1a-/- mice. Vehicle-treated mdr1a-/- mice were found to produce serum antibodies to antigens derived from members of the intestinal microbiota, indicative of severe colitis and a loss of adaptive tolerance to the members of the microbiota. These serum antibodies were greatly reduced or absent in P. vulga

关键词： Prunella vulgaris Spontaneous colitis Inflammatory

Basic Study Impact of stimulant medication on behaviour and executive functions in children with attention-deficit/hyperactivity disorder

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Pediatrics 2022年第1期11卷 48-60页

作者： Tasmia Hai Hanna A Duffy Julie anne Lemay Jean François Lemay Werklund School of education University of CalgaryCalgaryAB T2N 1N4Canada Department of Paediatrics Alberta Children's Hospital/Cumming School of MedicineUniversity of CalgaryCalgaryAB T3B 6A8Canada

BACKGROUND Children with attention-deficit/hyperactivity disorder(ADHD)often exhibit behaviour challenges and deficits in executive functions(eF).Psychostimulant medications[e.g.,methylphenidate(MPH)]are commonly prescribed for children with ADHD and are considered effective in 70%of the ***,only a handful of studies have investigated the long-term impact of MPH medication on eF and *** To evaluate behaviour and eF challenges in children with ADHD who were involved in an MPH treatment trial across three-time *** Thirty-seven children with ADHD completed a stimulant medication trial to study the short-and long-term impact of *** with ADHD completed three neuropsychological assessments[Continuous Performance Test(CPT)-II,Digit Span Backwards and Spatial Span Backwards].Parents of children with ADHD completed behaviour rating scales[Behaviour Rating Inventory of executive Functioning(BRIeF)and Behaviour Assessment System for Children-Second edition(BASC-2)].Participants were evaluated at:(1)Baseline(no medication);and(2)Best-dose(BD;following four-week MPH treatment).Additionally,18 participants returned for a long-term naturalistic follow up(FU;up to two years following BD).ReSULTS Repeated measure analyses of variance found significant effects of time on two subscales of BRIeF and four subscales of *** assessments showed some improvement,but not on all tasks following the medication *** improvements did not sustain at FU,with increases in eF and behaviour challenges,and a decline in performance on the CPT-II task being *** Parents of children with ADHD reported improvements in eF and behaviours during the MPH trial but were not sustained at *** screening tools and neuropsychological assessments may be useful for monitoring medication responses.

关键词： Attention-deficit/hyperactive disorder Behaviour executive functions Stimulant medications