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Cross-linked self-assembling peptide scaffolds

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Nano Research 2018年第1期11卷 586-602页

作者： Raffaele pugliese Amanda Marchini Gloria anna Ada Saracino1 Ronald N. Zuckermann3, Fabrizio Gelain IRCCS Casa Sollievo della Sofferenza Opera di San Pio da Pietralcina Viale Capuccini I San Giovanni Rotondo Foggia 71013 Italy Center for Nanomedicine and Tissue Engineering (CNTE) A. O. Ospedale Niguarda Cd Granda Piazza dell' Ospedale Maggiore 3 Milan 20162 Italy The Molecular Foundry Lawrence Berkeley National Laboratory 1 Cyclotron Road Berkeley California 94720 USA

Self-assembling peptides （SAPs） are synthetic bioinspired biomaterials that can be feasibly multi-functionalized for cell transplantation and/or drug delivery therapies. Despite their superior biocompatibility and ease of scaling-up for production, they are unfortunately hampered by weak mechanical properties due to transient non-covalent interactions among and within the self-assembled peptide chains, thus limiting their potential applications as fillers, hemostat solutions, and fragile scaffolds for soft tissues. Here, we have developed and characterized a cross-linking strategy that increases both the stiffness and the tailorability of SAP hydrogels, enabling the preparation of transparent flexible threads, discs, channels, and hemispherical constructs. Empirical and computational results, in close agreement with each other, confirmed that the cross-linking reaction does not affect the previously self-assembled secondary structures. In vitro tests also provided a first hint of satisfactory biocompatibility by favoring viability and differentiation of human neural stem cells. This work could bring self-assembling peptide technology to many applications that have been precluded so far, especially in regenerative medicine.

关键词： self-assembling peptide co-assembling peptide cross-linking sulfo-SMCC supramolecularself-assembly

Biological and molecular interplay between two viruses and powdery and downy mildews in two grapevine cultivars

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Horticulture Research 2020年第1期7卷 408-421页

作者： Giovanna Gilardi Walter Chitarra Amedeo Moine Monica Mezzalama Paolo Boccacci Massimo pugliese maria Lodovica Gullino Giorgio Gambino Centre of Competence for the Innovation in the Agro-Environmental sector(AGROINNOVA) University of TorinoLargo Paolo Braccini 210095 GrugliascoItaly Institute for Sustainable Plant Protection National Research Council(IPSP-CNR)Strada delle Cacce 7310135 TorinoItaly Research Centre for Viticulture and Enology Council for Agricultural Research and Economics(CREA-VE)Via XXVIII Aprile 2631015 ConeglianoItaly Department of Agricultural Forest and Food Sciences(DISAFA)University of TorinoLargo Paolo Braccini 210095 GrugliascoItaly

Grapevine may be affected simultaneously by several pathogens whose complex interplay is largely *** studied the effects of infection by two grapevine viruses on powdery mildew and downy mildew development and the molecular modifications induced in grapevines by their multiple *** fanleaf virus(GFLV)and grapevine rupestris stem pitting-associated virus(GRSPaV)were transmitted by in vitro-grafting to Vitis vinifera cv Nebbiolo and Chardonnay virus-free plantlets regenerated by somatic *** were then artificially inoculated in the greenhouse with either Plasmopara viticola or Erysiphe necator ***-infected plants showed a reduction in severity of the diseases caused by powdery and downy mildews in comparison to virus-free *** induced the overexpression of stilbene synthase genes,pathogenesis-related proteins,and influenced the genes involved in carbohydrate metabolism in *** transcriptional changes suggest improved innate plant immunity,which makes the GFLV-infected grapevines less susceptible to other biotic ***,however,cannot be extrapolated to GRSPaV as it was unable to promote protection against the fungal/oomycete *** these multiple interactions,the grapevine genotype seemed to have a crucial role:in‘Nebbiolo’,the virus-induced molecular changes were different from those observed in‘Chardonnay’,suggesting that different metabolic pathways may be involved in protection against fungal/oomycete *** results indicate that complex interactions do exist between grapevine and its different pathogens and represent the first study on a topic that still is largely unexplored.

关键词： mildew powdery cultivar

Anti-TNF alpha in the treatment of ulcerative colitis:A valid approach for organ-sparing or an expensive option to delay surgery?

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World Journal of Gastroenterology 2014年第17期20卷 4839-4845页

作者： Gianluca Rizzo Daniela pugliese Alessandro Armuzzi Claudio Coco Department of Surgical Sciences Complesso Integrato ColumbusCatholic University of the Sacred Heart00168 RomeItaly Internal Medicine and Gastroenterology Unit Complesso Integrato ColumbusCatholic University of the Sacred Heart00168 RomeItaly

Ulcerative colitis (UC) is an inflammatory bowel disease affecting large bowel with variable clinical course. The history of disease has been modified by the introduction of biologic therapy, in particular Infliximab (IFX), that has demonstrated efficacy in inducing fast symptoms remission, promoting mucosal healing and maintaining long-term remission. However, surgery is still needed for UC patients: in case of failure of medical therapy and if acute complications or a malignancy occurred. Surgical treatment is associated with a short-term post-operative mortality and morbidity respectively of 0%-4% and 30%. In this study we systematically analyzed: the role of IFX in reducing the colectomy rate, the risk of post-operative morbidity in pre-operatively IFX-treated patients and the cost-effectiveness of IFX therapy. Four of 5 analyzed randomized controlled trials demonstrated that therapy with IFX significantly reduces the colectomy rate. Moreover, pre-operative treatment with IFX doesn’t seem to increase post-operative infectious complications. By an economic point of view, the cost-effectiveness of IFX-therapy was demonstrated for UC patients suffering from moderate to severe UC in a study based on a cost estimation of the National Health Service of England and Wales. However, the argument is debated.

关键词： Ulcerative colitis Infliximab Colectomy Post-operative complications Cost-effectiveness Inflammatory bowel disease

Computer-aided sperm analysis： a useful tool to evaluate patient＇s response to varicocelectomy

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Asian Journal of Andrology 2017年第4期19卷 449-452页

作者： Julia I Ariagno Gabriela R Mendeluk maria J Furlan M Sardi P Chenlo Susana M Curi Mercedes N pugliese Herberto E Repetto mariano Cohen Clinical Biochemistry Department Male Fertility Laboratory Faculty of Pharmacy and Biochemetry University of Buenos Aires Argentina Urology Department "Jose de San Martin" Clinical Hospital University of Buenos Aires Buenos Aires Argentina (CP1120)

Preoperative and postoperative sperm parameter values from infertile men with varicocele were analyzed by computer-aided sperm analysis （CASA） to assess if sperm characteristics improved after varicocelectomy. Semen samples of men with proven fertility （n = 38） and men with varicocele-related infertility （n = 61） were also analyzed. Conventional semen analysis was performed according to WHO （2010） criteria and a CASA system was employed to assess kinetic parameters and sperm concentration. Seminal parameters values in the fertile group were very far above from those of the patients, either before or after surgery. No significant improvement in the percentage normal sperm morphology （P = 0. 10）, sperm concentration （P = 0.52）, total sperm count （P = 0.76）, subjective motility （%）（P = 0.97） nor kinematics （P = 0.30） was observed after varicocelectomy when all groups were compared. Neither was significant improvement found in percentage normal sperm morphology （P = 0.91）, sperm concentration （P =0. 10）, total sperm count （P = 0.89） or percentage motility （P = 0.77） after varicocelectomy in paired comparisons of preoperative and postoperative data. Analysis of paired samples revealed that the total sperm count （P = 0.01） and most sperm kinetic parameters： curvilinear velocity （P = 0.002）, straight-line velocity （P = 0.0004）, average path velocity （P = 0.0005）, linearity （P = 0.02）, and wobble （P = 0.006） improved after surgery. CASA offers the potential for accurate quantitative assessment of each patient＇s response to varicocelectomy.

关键词： computer-assisted semen analysis male infertility semen analysis varicocele varicocelectomy

Geobatteries in environmental biogeochemistry: Electron transfer and utilization

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Environmental Science and Ecotechnology 2024年第6期22卷 38-51页

作者： Shihao Cui Rui Wang Qing Chen Lorenzo pugliese Shubiao Wu Department of Agroecology Aarhus UniversityBlichers Allé208830TjeleDenmark Department of Biological and Chemical Engineering Aarhus UniversityGustav Wieds Vej 108000AarhusDenmark Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation College of Resources and Environmental SciencesChina Agricultural UniversityBeijing100193China

The efficiency of direct electron flow from electron donors to electron acceptors in redox reactions is significantly influenced by the spatial separation of these ***,a class of redox-active substances naturally present in soil-water systems,act as electron reservoirs,reversibly donating,storing,and accepting *** capability allows the temporal and spatial decoupling of redox half-reactions,providing a flexible electron transfer *** this review,we systematically examine the critical role of geobatteries in influencing electron transfer and utilization in environmental biogeochemical *** redox-active centers within geobatteries,such as quinone-like moieties,nitrogen-and sulfur-containing groups,and variable-valent metals,possess the potential to repeatedly charge and *** characterization techniques,ranging from qualitative methods like elemental analysis,imaging,and spectroscopy,to quantitative techniques such as chemical,spectroscopic,and electrochemical methods,have been developed to evaluate this reversible electron transfer ***,current research on the ecological and environmental significance of geobatteries extends beyond natural soil-water systems(e.g.,soil carbon cycle)to engineered systems such as water treatment(e.g.,nitrogen removal)and waste management(e.g.,anaerobic digestion).Despite these advancements,challenges such as the complexity of environmental systems,difficulties in accurately quantifying electron exchange capacity,and scaling-up issues must be addressed to fully unlock their *** review underscores both the promise and challenges associated with geobatteries in responding to environmental issues,such as climate change and pollutant transformation.

关键词： Geobattery Electron transfer Redox reactions

CO_2和温度升高情况下白粉菌侵染对西葫芦生长特性的影响

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生态学报 2011年第2期31卷 491-497页

作者：刘俊稚葛亚明 pugliese Massimo Garibaldi Angelo Gullino maria Lodovica 田光明浙江大学环境工程系 Centre of Competence in the Agro-environmental Sector(AGROINNOVA) University of TorinoGrugliasco 10095Italy

植物病害是降低植物产量和产品品质的重要因素,但对其在气候变化情景下如何影响植物的研究鲜见报道。利用封闭式人工气候室模拟不同环境处理,探讨了大气CO2浓度增加和温度升高情况下白粉菌(Podosphaera xanthii)侵染对西葫芦(Cucurbita ... 详细信息

植物病害是降低植物产量和产品品质的重要因素,但对其在气候变化情景下如何影响植物的研究鲜见报道。利用封闭式人工气候室模拟不同环境处理,探讨了大气CO2浓度增加和温度升高情况下白粉菌(Podosphaera xanthii)侵染对西葫芦(Cucurbita pepo)生长发育的影响。结果表明,单独CO2加富(EC)增强了西葫芦光合作用(P<0.05),促进了植株生长和果实生产;CO2浓度和温度同时升高(ECT)也促进了光合作用(P<0.05),加速了植株器官发育,但限制了叶片叶绿素合成和叶片面积生长,最终明显降低了植株地上部分干物质积累和果实产量(P<0.05)。和对照相比,EC处理下白粉菌的生长繁殖没有明显变化,但由于西葫芦植株的抗病性有所改善,植株病情指数略有下降;而ECT处理下白粉菌的发育繁殖明显改善,***菌落规模和产孢能力极大提高(P<0.01),植物病情指数显著加重(P<0.01),作物严重减产(P<0.01)。可见,在未来以CO2浓度和温度升高为特征的气候变化条件下,白粉菌倾向于加重对西葫芦的侵染。这个结论对其他葫芦科白粉病的防治管理也有借鉴作用。

关键词：全球气候变化西葫芦白粉菌植物病害生长特性

Risk of hepatitis B virus reactivation in oncological patients treated with tyrosine kinase inhibitors:A case report and literature analysis

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World Journal of Gastroenterology 2024年第9期30卷 1253-1256页

作者： Francesca Colapietro Nicola pugliese Antonio Voza Alessio Aghemo Stella De Nicola Department of Biomedical Sciences Humanitas UniversityMilan 20072Italy Division of Internal Medicine and Hepatology Department of GastroenterologyHumanitas Research HospitalMilan 20089Italy Department of Emergency Humanitas Research HospitalMilan 20089Italy

Hepatitis B virus(HBV)reactivation(HBVr)represents a severe and potentially life-threatening condition,and preventive measures are available through blood test screening or prophylactic therapy *** assessment of HBVr traditionally considers factors such as HBV profile,including hepatitis B surface antigen(HBsAg)and antibody to hepatitis B core antigen,along with type of medication(chemotherapy;immunomodulants).Nevertheless,consideration of possible patient’s underlying tumor and the specific malignancy type(solid or hematologic)plays a crucial role and needs to be assessed for decision-making process.

关键词： Chronic hepatitis B Reactivation Nucleoside analogue Tyrosine kinase inhibitors Onco-hematology

Osteosarcopenia in autoimmune cholestatic liver diseases:Causes,management,and challenges

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World Journal of Gastroenterology 2022年第14期28卷 1430-1443页

作者： Nicola pugliese Ivan Arcari Alessio Aghemo Andrea G Lania Ana Lleo Gherardo Mazziotti Department of Biomedical Sciences Humanitas UniversityPieve Emanuele 20090MilanItaly Division of Internal Medicine and Hepatology Department of GastroenterologyIRCCS Humanitas Research HospitalRozzano 20089MIItaly Department of Endocrinology Diabetology and Medical Andrology UnitIRCCS Humanitas Research HospitalRozzano 20089MIItaly

Primary biliary cholangitis and primary sclerosing cholangitis(PSC)are the most common cholestatic liver diseases(CLD)in adults and are both characterized by an immune *** primary biliary cholangitis is a model autoimmune disease,with over 90%of patients presenting very specific autoantibodies against mitochondrial antigens,PSC is considered an immune mediated *** is the most common bone disease in CLD,resulting in frequent fractures and leading to significant ***,sarcopenia is emerging as a frequent complication of chronic liver diseases with a significant prognostic impact and severe implications on the quality of life of *** mechanisms underlying osteoporosis and sarcopenia in CLD are still largely unknown and the association between these clinical conditions remains to be *** timely diagnosis,prevention,and management of osteosarcopenia are crucial to limit the consequences,there are no specific guidelines for management of osteoporosis and sarcopenia in patients with *** guidelines recommend screening for bone disease at the time of diagnosis of ***,the optimal monitoring strategies and treatments have not been defined yet and vary among *** herein aim to comprehensively outline the pathogenic mechanisms and clinical implications of osteosarcopenia in CLD,and to summarize expert recommendations for appropriate diagnostic and therapeutic approaches.

关键词： Cholestatic liver diseases Primary biliary cholangitis Primary sclerosing cholangitis Osteoporosis Sarcopenia

Indoor Radon Concentrations in Dwellings of Ischia Island

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Journal of Environmental Protection 2013年第8期4卷 37-39页

作者： mariagabriella pugliese maria Quarto Filomena Loffredo Agostino Mazzella Vincenzo Roca 1Dipartimento di Fisica Università di Napoli Federico II Naples Italy 2Istituto Nazionale di Fisica Nucleare (INFN) Sezione di Napoli Naples Italy Dipartimento di Fisica Università di Napoli Federico II Naples Italy Liceo Statale Ischia Naples Italy

Indoor radon concentration measurements were performed in dwellings of Ischia island (South Italy) using commercial E-Perm devices in LST configuration. The average concentrations in the dwellings were found to vary from 125 to 865 Bq/m3 with an average of 354 ± 176 Bq/m3. This value is higher than Italian National average that is 75 Bq/m3 and Campanian average that is 95 Bq/m3. Correlation between indoor radon concentration measurements and floor level of monitored room was also studied.

关键词： Radon E-Perm Floor Level