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β-catenin mRNA encapsulated in SM-102 lipid nanoparticles enhances bone formation in a murine tibia fracture repair model

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Bioactive Materials 2024年第9期39卷 273-286页

作者： anna laura nelson Chiara Mancino Xueqin Gao Joshua AChoe laura Chubb Katherine Williams Molly Czachor Ralph Marcucio Francesca Taraballi John P.Cooke Johnny Huard Chelsea Bahney Nicole Ehrhart Steadman Philippon Research Institute(SPRI) Center for Regenerative and Personalized MedicineVailCOUSA Houston Methodist Research Institute Center for Musculoskeletal RegenerationHouston TXUSA University of Wisconsin-Madison Department of Orthopedics and RehabilitationDepartment of Biomedical EngineeringMedical Scientist Training ProgramMadisonWIUSA Colorado State University School of Biomedical EngineeringFort Collins COUSA Colorado State University Department of Clinical SciencesFort Collins COUSA Colorado State University Department of MicrobiologyImmunologyand PathologyFort CollinsCOUSA Houston Methodist Research Institute Center for RNA TherapeuticsDepartment of Cardiovascular SciencesHoustonTXUSA University of Californi1 San Francisco(UCSF)Orthopaedic Trauma InstituteSan FranciscoCAUSA

Fractures continue to be a global economic burden as there are currently no osteoanabolic drugs approved to accelerate fracture *** this study,we aimed to develop an osteoanabolic therapy which activates the Wnt/β-catenin pathway,a molecular driver of endochondral *** hypothesize that using an mRNAbased therapeutic encodingβ-catenin could promote cartilage to bone transformation formation by activating the canonical Wnt signaling pathway in *** optimize a delivery platform built on recent advancements in liposomal technologies,two FDA-approved ionizable phospholipids,DLin-MC3-DMA(MC3)and SM-102,were used to fabricate unique ionizable lipid nanoparticle(LNP)formulations and then tested for transfection efficacy both in vitro and in a murine tibia fracture *** firefly luciferase mRNA as a reporter gene to track and quantify transfection,SM-102 LNPs showed enhanced transfection efficacy in vitro and prolonged transfection,minimal fracture interference and no localized inflammatory response in vivo over MC3 *** generatedβ-cateninGOF mRNA encapsulated in SM-102 LNPs(SM-102-β-cateninGOF mRNA)showed bioactivity in vitro through upregulation of downstream canonical Wnt genes,axin2 and *** testing SM-102-β-cateninGOF mRNA therapeutic in a murine tibia fracture model,histomorphometric analysis showed increased bone and decreased cartilage composition with the 45μg concentration at 2 weeks post-fracture.μCT testing confirmed that SM-102-β-cateninGOF mRNA promoted bone formation in vivo,revealing significantly more bone volume over total volume in the 45μg ***,we generated a novel mRNA-based therapeutic encoding aβ-catenin mRNA and optimized an SM-102-based LNP to maximize transfection efficacy with a localized delivery.

关键词： Gene therapy Fracture healing Lipid nanoparticles mRNA Canonical Wnt

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Britain Seeks Compromise on Animal Research

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当代外语研究 2004年第9期 19-20页

作者： laura nelson 陈青

利用动物进行药物活体试验似乎一直被人们认为是天经地义的。然而在英国,人们已经开始注意保护the welfare of lab animals,这方面的极端分子甚至对有关实验室的研究工作人员发动了攻击,毁坏有关设施。面对这一局面,英国政府正在努力采... 详细信息

利用动物进行药物活体试验似乎一直被人们认为是天经地义的。然而在英国,人们已经开始注意保护the welfare of lab animals,这方面的极端分子甚至对有关实验室的研究工作人员发动了攻击,毁坏有关设施。面对这一局面,英国政府正在努力采取措施,希望情况能够有所改善。

关键词： Britain Seeks Compromise on Animal Research

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Nasal Solution Joins SARS Race

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当代外语研究 2004年第8期 2-3页

作者： laura nelson 秦艳艳

SARS如今已引起人们的普遍关注。我们不会忘记在过去两年中,SARS给我们带来的巨大损失和灾难。如今SARS暂时得到了有效的控制,但谁也不能保证它不会再次卷土重来。现在科学家们正致力于研制SARS疫苗,以求从根本意义上解决问题。本文对... 详细信息

SARS如今已引起人们的普遍关注。我们不会忘记在过去两年中,SARS给我们带来的巨大损失和灾难。如今SARS暂时得到了有效的控制,但谁也不能保证它不会再次卷土重来。现在科学家们正致力于研制SARS疫苗,以求从根本意义上解决问题。本文对研究过程中的尝试及新发现做了介绍。

关键词： Nasal Solution Joins SARS Race

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An integrated approach for increasing breeding efficiency in apple and peach in Europe

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Horticulture Research 2018年第1期5卷 825-838页

作者： Francois Laurens Maria JoséAranzana Pere Arus Daniele Bassi Marco Bink Joan Bonany Andrea Caprera Luca Corelli-Grappadelli Evelyne Costes Charles-Eric Durel Jehan-Baptiste Mauroux Hélène Muranty nelson Nazzicari Thierry Pascal Andrea Patocchi Andreas Peil Bénédicte Quilot-Turion laura Rossini Alessandra Stella Michela Troggio Riccardo Velasco Eric van de Weg IRHS INRAAgrocampus-OuestUniversitéd’AngersSFR 4207 QuaSaVUniversitéBretagne Loire42 rue Georges MorelBeaucouzé49071France IRTA(Institut de Recerca i Tecnologia Agroalimentàries) BarcelonaSpain Centre for Research in Agricultural Genomics(CRAG)CSIC-IRTA-UAB-UB Campus UABBellaterraBarcelonaSpain Universitàdegli Studi di Milano-DiSAA Via Celoria 2Milan20133Italy Biometris Wageningen University and ResearchDroevendaalsesteeg 1Wageningen6708PBThe Netherlands IRTA-Mas Badia Mas BadiaLa Tallada17134Spain Parco Tecnologico Padano Via EinsteinLoc.Cascina CodazzaLodi26900Italy Dipartimento di Scienze Agrarie Alma Mater Studiorum-Universitàdi BolognaBolognaItaly UMR AGAP INRAMontpellier34398France GAFL INRAMontfavet84143France Agroscope Research Division Plant BreedingSchloss 1Wädenswil8820Switzerland Julius Kühn-Institute(JKI) Federal Research Centre for Cultivated Plants Institute for Breeding Research on Fruit CropsPillnitzer Platz 3aDresden01326Germany Research and Innovation Center Fondazione Edmund MachSan Michele all’AdigeTrentoItaly CREA-VE Center of Viticulture and Enologyvia XXVIII Aprile 26Conegliano(TV)31015Italy Plant Breeding Wageningen University and ResearchDroevendaalsesteeg 1P.O.Box 386Wageningen6700AJThe Netherlands Hendrix Genetics Research Technology&ServicesBoxmeer5830 ACThe Netherlands

Despite the availability of whole genome sequences of apple and peach,there has been a considerable gap between genomics and *** bridge the gap,the European Union funded the FruitBreedomics project(March 2011 to August 2015)involving 28 research institutes and private *** complementary approaches were pursued:(i)tool and software development,(ii)deciphering genetic control of main horticultural traits taking into account allelic diversity and(iii)developing plant materials,tools and methodologies for *** breakthroughs were made including the making available of ready-to-go DNA diagnostic tests for Marker Assisted Breeding,development of new,dense SNP arrays in apple and peach,new phenotypic methods for some complex traits,software for gene/QTL discovery on breeding germplasm via Pedigree Based Analysis(PBA).This resulted in the discovery of highly predictive molecular markers for traits of horticultural interest via PBA and via Genome Wide Association Studies(GWAS)on several European genebank *** also developed pre-breeding plant materials in which multiple sources of resistance were pyramided and software that can support breeders in their selection *** FruitBreedomics,significant progresses were made in the field of apple and peach breeding,genetics,genomics and bioinformatics of which advantage will be made by breeders,germplasm curators and scientists.A major part of the data collected during the project has been stored in the FruitBreedomics database and has been made available to the *** review covers the scientific discoveries made in this major endeavour,and perspective in the apple and peach breeding and genomics in Europe and beyond.

关键词： breeding germplasm breed

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布鲁斯·威利斯对抗失语症

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空中英语教室（高级版．彭蒙惠英语） 2022年第10期 8-9,38,39页

作者： laura J.nelson Thomas Curwen Emily Baumgaertner 陈信宏(译) 不详

罹患失语症被描述为就像住在一个讲陌生语言的国度里。“你了解眼前的情况。你也是原来的你——但别人不知道,”加拿大麦克马斯特大学语言病理学与神经科学教授琳恩·特克斯特拉说,“突然间,你没办法像过去那样表达自己的想法与感受,如... 详细信息

罹患失语症被描述为就像住在一个讲陌生语言的国度里。“你了解眼前的情况。你也是原来的你——但别人不知道,”加拿大麦克马斯特大学语言病理学与神经科学教授琳恩·特克斯特拉说,“突然间,你没办法像过去那样表达自己的想法与感受,如果这种情况是渐进式的,那么你就会感觉到自己的表达能力在慢慢消失。”

关键词：布鲁斯神经科学麦克马斯特大学失语症威利斯斯特拉表达能力渐进式

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酒店改造如何实现新旧、内外的统一——葡萄牙米尼奥酒店改造与扩建

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设计家 2015年第1期 60-65页

作者： JP Pereira Pedro Guedes Teresa Aguiar Leonor Macedo Eva Sousa nelson Garrido Vírgula i

米尼奥酒店在原有建筑基础上重新设计了公共区和水疗中心,并扩建了酒店面积。新设计保持了原建筑简洁朴素的特点,同时又提升了酒店形象。酒店建筑决定了室内设计必将是整个设计非常重要的一部分,建筑是外壳,室内是核心,内外之间的差异... 详细信息

米尼奥酒店在原有建筑基础上重新设计了公共区和水疗中心,并扩建了酒店面积。新设计保持了原建筑简洁朴素的特点,同时又提升了酒店形象。酒店建筑决定了室内设计必将是整个设计非常重要的一部分,建筑是外壳,室内是核心,内外之间的差异被有意地简化成单一的解决方案。由于建筑原本就存在,扩建建筑在外观上要保持原有特征。

关键词：原有建筑米尼室内设计公共区设计过程主设计师 laura 内外空间表达情绪周围环境

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Turbo-taxonomy to assemble a megadiverse lichen genus:seventy new species of Cora(Basidiomycota:Agaricales:Hygrophoraceae),honouring David Leslie Hawksworth’s seventieth birthday

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Fungal Diversity 2017年第3期84卷 139-207页

作者： Robert Lücking Manuela Dal Forno Bibiana Moncada Luis Fernando Coca Leidy Yasmín Vargas-Mendoza André Aptroot laura Juliana Arias Betty Besal Frank Bungartz Diego Mauricio Cabrera-Amaya Marcela E.S.Cáceres José Luis Chaves Sionara Eliasaro Martha Cecilia Gutiérrez Jesús E.Hernández Marin María de los Ángeles Herrera-Campos María E.Holgado-Rojas Harald Jonitz Martin Kukwa Fabiane Lucheta Santiago Madriñán Marcelo Pinto Marcelli Suzana Maria de Azevedo Martins Joel A.Mercado-Díaz Jorge Alberto Molina Eduardo A.Morales Peter R.nelson Freddy Nugra Francisco Ortega Telma Paredes Ayda Lucía Patiño Rouchi Nadine Peláez-Pulido Rosa Emilia Pérez Pérez Gary B.Perlmutter Eimy Rivas-Plata Javier Robayo Camilo Rodríguez Diego Fernando Simijaca Edier Soto-Medina Adriano Afonso Spielmann Alejandra Suárez-Corredor Jean-Marc Torres Carlos Alberto Vargas Alba Yánez-Ayabaca Gothamie Weerakoon Karina Wilk Marcela Celis Pacheco Mauricio Diazgranados Grischa Brokamp Thomas Borsch Patrick M.Gillevet Masoumeh Sikaroodi James D.Lawrey Botanical Garden and Botanical Museum Berlin KöniginLuise-Straße 6-814195 BerlinGermany Science Education The Field Museum1400 South Lake ShoreChicagoIL 60605USA Department of Botany Smithsonian InstitutionNational Museum of Natural History10th St Constitution Ave NWWashingtonDC 20560USA Department of Environmental Science and Policy George Mason UniversityFairfaxVA 22030-4444USA Licenciatura en Biologı´a Universidad Distrital Francisco Jose´de CaldasCra.4 No.26D-54Torre de LaboratoriosHerbarioBogota´D.C.Colombia Herbario Universidad de Caldas(FAUC) Edificio BicentenarioCalle 65No.26-10ManizalesA.A.275CaldasColombia ABL Herbarium G.v.d.Veenstraat 1073762 XK SoestThe Netherlands Biologı´a Universidad del Quindı´oCarrera 15 Calle 12 NorteArmeniaQuindı´oColombia 120 Chavis Avenue LexingtonVA 24450USA Charles Darwin Foundation Puerto AyoraSanta Cruz IslandGala´pagosEcuador Schedestraße 8 53113 BonnGermany Jardı´n Bota´nico de Bogota´Jose´Celestino Mutis Avenida Calle 63 No.68-95Bogota´D.C.Colombia Departamento de Biocieˆncias Universidade Federal de SergipeAvenida Vereador Olı´mpio Grandes/n.ItabaianaCEP:49.500-000 SergipeBrazil Laboratorio de Hongos Instituto Nacional de Biodiversidad(INBio)Apdo.22-3100Santo Domingo de HerediaCosta Rica Departamento de Botaˆnica Universidade Federal do Parana´Campus Polite´cnicoSetor de Cieˆncias Biolo´gicasAv.Cel.Francisco H.dos Santoss/nJardim das Ame´ricas81531-980 CuritibaPRBrazil Ingenierı´a Ambiental Universidad Distrital Francisco Jose´de CaldasCarrera 5 Este No.15-82Av.CircumvalarBogota´D.C.Colombia Instituto Experimental Jardı´n Bota´nico Dr.Tobı´as Lasser Centro de Investigacio´n y DesarrolloUniversidad Central de VenezuelaAve.Salvador AllendeJardı´n Bota´nico de CaracasApartado#2156Caracas 1010-AVenezuela Departamento de Bota´nica Instituto de Biologı´aUniversidad Nacional Auto´noma de Me´xicoApartado Postal 70-36704510 Ciudad De Me´xicoMexico Facultad de Ciencias Escuela Profesional de Biologı´aUniv

Following a large-scale phylogenetic study of the lichenized genus Cora(Basidiomycota:Agaricales:Hygrophoraceae),we formally describe 70 new species,honouring the seventieth birthday of David Leslie Hawksworth,one of the preeminent figures in mycology and lichenology in the past 50 *** on an updated phylogeny using the ITS fungal barcoding locus,we now recognize 189 taxa in a genus that until recently was considered to represent a single species;including this contribution,92 of these are formally recognized,including five taxa based on historical names or collections that have not been *** of Cora can be recognized by a combination of morphological(size,colour,lobe configuration,surface hairs,hymenophore size and shape),anatomical(thallus thickness,cortex structure,photobiont type,hyphal papillae),and ecogeographical features(substrate,habitat,distribution),and a keytable allowing the identification of all accepted taxa is *** new species are:Cora accipiter Moncada,Madrin˜a´n&Lücking ***.,*** Moncada,Soto-Medina&Lücking ***.,*** Moncada,Dal Forno&Lücking ***.,*** Dal Forno,Chaves&Lücking ***.,*** Moncada,***ı´guez&Lücking ***.,*** Lücking,Moncada&*** ***.,*** Moncada,Ya´nez-Ayabaca&Lücking ***.,*** Moncada,Patin˜o&Lücking ***.,*** Lücking,***&Dal Forno ***.,*** Holgado,Rivas Plata&Perlmutter ***.,*** Dal Forno,Eliasaro&Spielmann ***.,*** Nugra,Dal Forno&Lücking ***.,*** Lücking,Martins&Lucheta ***.,*** Moncada,R.-***´rez&Lücking ***.,*** Moncada,***.&Lücking ***.,*** Moncada,CabreraAmaya&Lücking ***.,*** Moncada,R.-***´rez&***.,*** Lücking,***&Dal Forno ***.,*** Moncada,***´rez-Corredor&Lücking ***.,*** Moncada,***&Lücking ***.,***

关键词： Bolivia Citizen science Chile Mexico Puerto Rico Sri Lanka

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Does Dust from Arctic Mines Affect Caribou Forage?

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Journal of Environmental Protection 2017年第3期8卷 258-276页

作者： Wenjun Chen Sylvain G. Leblanc H. Peter White Christian Prevost Brian Milakovic Christine Rock Greg Sharam Harry O’Keefe laura Corey Bruno Croft Anne Gunn Sjoerd van der Wielen Adeline Football Boyan Tracz Jody Snortland Pellissey John Boulanger Canada Centre for Remote Sensing Natural Resources Canada Ottawa Canada ERM Environmental Ressources Management Vancouver Canada Dominion Diamond Ekati Corporation Yellowknife Canada Environment and Natural Resources GNWT Yellowknife Canada Circum Arctic Rangifer Monitoring and Assessment Network Salt Spring Island Canada Tlicho Government Behchoko NT Canada Wek’èezhìi Renewable Resources Board Yellowknife Canada Integrated Ecological Research Ltd. Nelson Canada

This study explores how dust from the Ekati Diamond Mine potentially affects the availability and quality of forage on the seasonal range of the Bathurst caribou herd. Understanding the effects of dust as a source of disturbance is important because the Bathurst caribou population has declined by 93% since the middle 1980s and there are reports that caribou in general may avoid mining projects. There are several challenges for quantifying dust impacts: 1) Natural variations (e.g., topography, natural disturbance, and soil pH) may also impact forage availability and quality for caribou. To minimize their masking effect, we stratified survey sites into seven land cover classes and selected the most populous class (i.e., the dwarf shrub) for assessing the impact. 2) Within class variation (e.g., the proportion of area covered by rocks where vascular plants and lichen do not grow) can further skew the analysis. We eliminated this problem by examining only the area not covered by rocks. 3) Coarse and fine suspended particulates have different spatial coverages, chemical compositions, and pH values. Consequently, their impacts on caribou forage can be different. To distinguish their impacts, we sampled two areas: transects from the Misery Haul Road that has been in active use vs. those from a rarely used spur road outside the Misery Camp. We sampled percent vegetation cover, soil pH, and dust on leaves along these transects during the summers of 2015 and 2016. Our results indicated that the amount of dust on leaves in a zone of ~1000 m from the Misery Haul Road was 3 - 9 times than that of background sites. The zone of reduced lichen percent cover was also about 1000 m. In contrast, these road dust-induced changes in caribou forage were not observed for the dust-free transect from the spur road.

关键词： Mining Arctic Caribou Habitat Road Dust PM2.5 Vegetation Cover Lichen Soil pH Dust Deposition

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多器官床旁即时超声在新型冠状病毒肺炎中的应用:国际专家共识

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中国呼吸与危重监护杂志 2021年第2期20卷 77-84页

作者：王臻(译) Arif Hussain Gabriele Via Lawrence Melniker Alberto Goffi Guido Tavazzi Luca Neri Tomas Villen Richard Hoppmann Francesco Mojoli Vicki Noble Laurent Zieleskiewicz Pablo Blanco Irene W.Y.Ma Mahathar Abd.Wahab Abdulmohsen Alsaawi Majid AI Salamah Martin Balik Diego Barca Karim Bendjelid Belaid Bouhemad Pablo Bravo-Figueroa Raoul Breitkreutz Juan Calderon Jim Connolly Roberto Copetti Francesco Corradi Anthony J.Dean AndréDenault Deepak Govil Carmela Graci Young-Rock Ha laura Hurtado Toru Kameda Michael Lanspa Christian B Laursen Francis Lee Rachel Liu Massimiliano Meineri Miguel Montorfano Peiman Nazerian Bret P.nelson Aleksandar N.Neskovic Ramon Nogue Adi Osman JoséPazeli Elmo Pereira-Junior Tomislav Petrovic Emanuele Pivetta Jan Poelaert Susanna Price Gregor Prosen Shalim Rodriguez Philippe Rola Colin Royse Yale Tung Chen Mike Wells Adrian Wong Wang Xiaoting Wang Zhen Yaseen Arabi 中国人民解放军空军军医大学航空航天医学系陕西西安710032 Department of Cardiac Sciences King Abdulaziz Medical City and King Abdullah International Medical Research Center 不详

新型冠状病毒肺炎(简称新冠肺炎,COVID-19)自首次报道以来,已造成全球超一亿人感染,给各国的公共卫生体系造成了巨大威胁。新冠肺炎的临床表现以呼吸系统体征和症状为主,80%的患者症状轻微,约5%为危重症患者,常表现为急性呼吸窘迫综合征... 详细信息

新型冠状病毒肺炎(简称新冠肺炎,COVID-19)自首次报道以来,已造成全球超一亿人感染,给各国的公共卫生体系造成了巨大威胁。新冠肺炎的临床表现以呼吸系统体征和症状为主,80%的患者症状轻微,约5%为危重症患者,常表现为急性呼吸窘迫综合征(ARDS)。前期,由于缺乏相关研究,面对日益紧迫的临床困境,医务人员不得不借鉴采取一些经验性措施以应对疫情。

关键词：体征和症状肺炎公共卫生体系呼吸系统首次报道专家共识疫情

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