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钢材洁净化技术相关中国专利分析与探讨

四川冶金 2024年第2期46卷 13-17页

作者：贾安娜王俊海王强钢铁研究总院有限公司/科技信息与战略研究所北京100081 冶金工业信息标准研究院/冶金信息研究所北京100730

本文通过专利数据库检索了2008年1月1日到2022年12月31日的钢材洁净化技术中国专利文献,以此为分析样本,采用定量分析方法,从申请量、申请人、发明人、法律状态等方面进行分析;而后分析了专利的技术领域、专利被引用情况;最后对重要相... 详细信息

本文通过专利数据库检索了2008年1月1日到2022年12月31日的钢材洁净化技术中国专利文献,以此为分析样本,采用定量分析方法,从申请量、申请人、发明人、法律状态等方面进行分析;而后分析了专利的技术领域、专利被引用情况;最后对重要相关专利做了详细介绍。结果表明:(1)近十几年来中国钢材洁净化专利申请量一直处于增长趋势;(2)专利申请主要集中在大型钢铁企业、冶金类高校及研究所;(3)钢中S、P、O、N、H的去除工艺技术不断提高但逐渐接近极限水平,针对不同品种和性能需要的钢中夹杂物的去除与控制技术发展迅速,洁净钢技术设备及装置的提升进一步促进了钢水的洁净化,洁净钢耐材的发展也促进钢材洁净化、耐侵蚀和长寿命。

关键词：钢材洁净化专利分析定量分析

富含半胱氨酸和甘氨酸蛋白2在神经母细胞瘤恶性进展中的功能和机制

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北京大学学报（医学版） 2024年第3期56卷 495-504页

作者：张瑶郭金鑫战世佳洪恩宇杨慧贾安娜常艳郭永丽张璇国家儿童医学中心首都医科大学附属北京儿童医院儿科重大疾病研究教育部重点实验室北京市儿科研究所儿童耳鼻咽喉头颈外科疾病北京市重点实验室北京100045

目的:探究富含半胱氨酸和甘氨酸蛋白2(cysteine and glycine-rich protein 2,CSRP2)在神经母细胞瘤(neuroblastoma,NB)恶性进展中的功能和作用机制。方法:利用R2数据库分析NB临床样本中CSRP2基因的mRNA水平与NB患儿临床预后的相关性;在N... 详细信息

目的:探究富含半胱氨酸和甘氨酸蛋白2(cysteine and glycine-rich protein 2,CSRP2)在神经母细胞瘤(neuroblastoma,NB)恶性进展中的功能和作用机制。方法:利用R2数据库分析NB临床样本中CSRP2基因的mRNA水平与NB患儿临床预后的相关性;在NB细胞系SK-N-BE(2)和SH-SY5Y中利用靶向小干扰RNA(small interfering RNA,siRNA)干扰CSRP2的表达或利用质粒转染过表达CSRP2;通过结晶紫染色和实时无标记动态细胞分析技术观察NB细胞的增殖情况;采用克隆形成方法观察NB细胞长时间的克隆形成能力;利用免疫荧光实验检测细胞增殖标记物Ki-67的水平;利用碘化丙啶(propidium iodide,PI)染色流式细胞术分析细胞周期比例,Annexin V/7AAD染色分析细胞凋亡比例;采用划痕实验观察细胞的迁移能力;利用Western blot或实时荧光定量PCR(quantitative real-time PCR,RT-qPCR)检测NB原发肿瘤组织和细胞系中蛋白和基因的表达水平。结果:NB临床数据库中,国际神经母细胞瘤分期(international neuroblastoma staging system,INSS)为高危险度3/4期的NB组织中CSRP2的mRNA水平显著高于低危险度的1/2期,且高表达水平组NB患儿的生存期显著低于低表达组;Western blot结果显示,CSRP2在3/4期NB组织中的蛋白水平显著高于1/2期。NB细胞中敲低CSRP2,细胞的活力减弱、增殖能力降低;NB细胞中过表达CSRP2促进细胞增殖;敲低CSRP2后,sub-G1、G0/G1和S期细胞的比例增加,Annexin V阳性细胞的比例增多;敲低CSRP2的NB细胞的划痕愈合率显著小于对照组。机制研究发现,敲低CSRP2后细胞增殖标记分子Ki-67和细胞外信号调节激酶1/2(extracellular signal-regulated kinases 1/2,ERK1/2)磷酸化水平显著低于对照组。结论:CSRP2在高危险度3/4期NB组织中高表达,表达水平与NB患儿生存期呈负相关;CSRP2通过促进ERK1/2活化,促进NB细胞的增殖和迁移,抑制细胞凋亡,表明CSRP2通过激活ERK1/2促进NB进展,为高危NB的靶向治疗提供了潜在的靶点。

关键词：富含半胱氨酸和甘氨酸蛋白2 神经母细胞瘤细胞增殖细胞迁移细胞外信号调节激酶1/2

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TMEFF1对神经母细胞瘤细胞系增殖的调控

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基础医学与临床 2024年第5期44卷 637-644页

作者：贾安娜郭金鑫张璇战世佳于永波郭永丽常艳国家儿童医学中心、首都医科大学附属北京儿童医院、儿科重大疾病研究教育部重点实验室、北京市儿科研究所儿童耳鼻咽喉头颈外科疾病北京市重点实验室北京100045

目的探究具有表皮生长因子结构域和两个卵泡抑素样结构域的跨膜蛋白1(TMEFF1/tomoregulin1)对神经母细胞瘤(NB)细胞系SN-K-BE(2)增殖和迁移的调控效应。方法RT-qPCR检测神经母细胞瘤细胞系SN-K-BE(2)、IMR32、SK-N-SH、SK-N-AS和正常细... 详细信息

目的探究具有表皮生长因子结构域和两个卵泡抑素样结构域的跨膜蛋白1(TMEFF1/tomoregulin1)对神经母细胞瘤(NB)细胞系SN-K-BE(2)增殖和迁移的调控效应。方法RT-qPCR检测神经母细胞瘤细胞系SN-K-BE(2)、IMR32、SK-N-SH、SK-N-AS和正常细胞系MCF 10A、hTERT RPE-1中TMEFF1 mRNA表达量。利用小分子干扰RNA(siRNA)瞬时敲低MCF 10A、hTERT RPE-1细胞和SN-K-BE(2)NB细胞中TMEFF1表达,RT-qPCR检测TMEFF1 mRNA瞬时敲低效果后,结晶紫染色、实时无标记细胞分析(RTCA)检测细胞增殖;CellTiter-Glo(CTG)检测细胞活性。利用短发夹RNA(shRNA)稳定敲低正常细胞系和NB细胞系中TMEFF1表达,进一步通过集落形成实验、RTCA、CTG检测细胞增殖和活性,此外,通过免疫荧光检测Ki-67细胞阳性率,细胞划痕实验检测细胞迁移率。结果与正常细胞系相比,NB细胞系中TMEFF1 mRNA表达量显著较高(P<0.001)。敲低TMEFF1对正常细胞系增殖影响无统计学意义,但在SN-K-BE(2)细胞中敲低TMEFF1,RTCA和集落形成实验结果显示细胞增殖能力减弱(P<0.05),免疫荧光结果显示Ki-67阳性细胞率减少(P<0.001),CTG结果显示细胞活性降低(P<0.01),细胞划痕实验结果显示敲低组细胞迁移率显著低于对照组(P<0.01)。结论TMEFF1是一种特异在NB细胞中高表达的膜蛋白,TMEFF1敲低后不影响正常细胞系的增殖,但显著抑制NB细胞系的增殖、迁移,提示TMEFF1可能在NB发生发展中发挥重要作用,可能成为NB靶向治疗的潜在新靶点。

关键词：神经母细胞瘤表皮生长因子结构域和两个卵泡抑素样结构域的跨膜蛋白1(TMEFF1/tomoregulin1) 增殖迁移

C12ORF66对MYCN扩增的高危神经母细胞瘤细胞的活性调控

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基础医学与临床 2024年第3期44卷 288-294页

作者：贾安娜战世佳张璇郭金鑫于永波郭永丽常艳国家儿童医学中心、首都医科大学附属北京儿童医院、儿科重大疾病研究教育部重点实验室、北京市儿科研究所儿童耳鼻咽喉头颈外科疾病北京市重点实验室北京100045

目的探究12号染色体开放阅读框66(C12ORF66)对MYCN扩增神经母细胞瘤(NB)细胞系活性的调控效应。方法通过R2数据库GSE16476和GSE49710数据集,分析MYCN扩增和MYCN非扩增NB细胞中C12ORF66表达量,以及C12ORF66表达量与患儿预后的相关性。RT-... 详细信息

目的探究12号染色体开放阅读框66(C12ORF66)对MYCN扩增神经母细胞瘤(NB)细胞系活性的调控效应。方法通过R2数据库GSE16476和GSE49710数据集,分析MYCN扩增和MYCN非扩增NB细胞中C12ORF66表达量,以及C12ORF66表达量与患儿预后的相关性。RT-qRCR检测正常组织永生化细胞系、MYCN扩增及MYCN非扩增细胞系中C12ORF66 mRNA表达差异。构建瞬时敲低和稳定敲低C12ORF66的MYCN扩增细胞株,对照组和敲低组细胞进行实时无标记动态细胞分析(RTCA)、集落形成能力检测及Ki67免疫荧光染色。结果R2数据库分析显示MYCN扩增NB患儿样本中C12ORF66表达显著高于MYCN非扩增NB患儿样本,并且C12ORF66表达与患儿预后负相关(P<0.05)。细胞实验表明,与MYCN非扩增细胞系CHLA-255和SH-SY5Y相比,C12ORF66在MYCN扩增细胞系BE(2)-C和SK-N-BE(2)中表达显著升高(P<0.001)。瞬时或稳定敲低C12ORF66,MYCN扩增NB细胞增殖显著减缓(P<0.001),集落形成能力显著降低(P<0.001),Ki67阳性细胞比例显著减少(P<0.05)。结论C12ORF66在MYCN扩增NB患儿样本和细胞系中高表达,且与患儿预后负相关,敲低C12ORF66显著抑制NB细胞活性。

关键词： 12号染色体开放阅读框66 SK-N-BE(2)细胞 MYCN扩增细胞增殖

Crucial role of histone deacetylase SIRT1 in myeloid-derived suppressor cell-mediated reprogramming of CD4^(+) T-cell differentiation

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Cellular & Molecular Immunology 2020年第7期17卷 785-787页

作者： Lin Dong Yujing Bi anna jia Qing Yu Yuexin Wang Yufei Wang Qiuli Yang Yejin Cao Ying He Ruichen Liu Yan Li Guangwei Liu Key Laboratory of Cell Proliferation and Regulation Biology Ministry of EducationInstitute of Cell BiologyCollege of Life SciencesBeijing Normal University100875 BeijingChina State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology100071 BeijingChina

Myeloid-derived suppressor cells(MDSCs)are heterogeneous,immature myeloid cells that inhibit the immune responses of T cells.1–5 MDSCs play a crucial role in infection,6,7 transplantation,8,9 autoimmune diseases10,11,and tumors12,13 by driving the differentiation of specific T-cell subsets,but the precise regulatory mechanism is still ***,our results showed that SIRT1,a histone deacetylase,suppresses the functions of proinflammatory MDSCs and promotes tumor growth.14 However,it remains unclear whether SIRT1 regulates the MDSC-induced differentiation of T ***,we identified the regulatory effects of SIRT1 in CD11b+Gr1+MDSCs on T-cell differentiation.

关键词： SIRT1 CD11b myeloid

Glucocorticoid receptor promotes the function of myeloid-derived suppressor cells by suppressing HIF1α-dependent glycolysis

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Cellular & Molecular Immunology 2018年第6期15卷 618-629页

作者： Yun Lu Huanrong Liu Yujing Bi Hui Yang Yan Li jian Wang Zhengguo Zhang Yu Wang Chunxiao Li anna jia Linian Han Ying Hu Yong Zhao Ruoning Wang Guangwei Liu Department of Immunology School of Basic Medical SciencesFudan UniversityShanghai 200032China Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education Institute of Cell BiologyCollege of Life SciencesBeijing Normal UniversityBeijing 100875China State Key laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Center for Childhood Cancer and Blood Diseases Hematology/Oncology and BMTThe Research Institute at Nationwide Children's HospitalOhio State UniversityColumbusOhio 43205USA

Immunomodulatory signaling imposes tight regulations on metabolic programs within immune cells and consequentially determines immune response *** the glucocorticoid receptor(GR)has been recently implicated in regulating the function of myeloid-derived suppressor cells(MDSCs),whether the dysregulation of GR in MDSCs is involved in immune-mediated hepatic diseases and how GR regulates the function of MDSCs in such a context remains ***,we revealed the dysregulation of GR expression in MDSCs during innate immunological hepatic injury(IMH)and found that GR regulates the function of MDSCs through modulating HIF1α-dependent *** modulation of GR by its agonist(dexamethasone,Dex)protects IMH mice against inflammatory ***,GR signaling suppresses HIF1αand HIF1α-dependent glycolysis in MDSCs and thus promotes the immune suppressive activity of *** studies reveal a role of GR-HIF1αin regulating the metabolism and function of MDSCs and further implicate MDSC GR signaling as a potential therapeutic target in hepatic diseases that are driven by innate immune cell-mediated systemic inflammation.

关键词： glucocorticoid-receptor glycolysis innate immunity liver injuries metabolism myeloid-derived suppressive cells tolerance

The kinase AKT1 potentiates the suppressive functions of myeloid-derived suppressor cells in inflammation and cancer

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Cellular & Molecular Immunology 2021年第4期18卷 1074-1076页

作者： anna jia Yuexin Wang Yufei Wang Yan Li Qiuli Yang Yejin Cao Ying He Lin Dong Yingjie Dong Yueru Hou Ruoning Wang Yujing Bi Guangwei Liu Key Laboratory of Cell Proliferation and Regulation Biology Ministry of EducationInstitute of Cell BiologyCollege of Life SciencesBeijing Normal UniversityBeijing 100875China Center for Childhood Cancer&Blood Diseases The Research Institute at Nationwide Children'sHospitalOhio State UniversityColumbusOH 43205USA State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China

Myeloid-derived suppressor cells(MDSCs)are a group of heterogeneous cells1 that play crucial negative regulatory roles in immune-associated diseases,including infections,2 cancer,3 transplantation4,and autoimmunity.5 We and others have shown that mTOR signaling and its downstream metabolic pathways regulate MDSC recruitment and function in inflammation and autoimmunity.

关键词： inflammation suppressor cancer

Extending Structures for Gel'fand-Dorfman Bialgebras

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Acta Mathematica Sinica,English Series 2024年第2期40卷 619-638页

作者： jia jia WEN Yan Yong HONG School of Mathematics Hangzhou Normal UniversityHangzhou 311121P.R.China

Gel'fand-Dorfman bialgebra,which is both a Lie algebra and a Novikov algebra with some compatibility condition,appeared in the study of Hamiltonian pairs in completely integrable *** also emerged in the description of a class special Lie conformal algebras called quadratic Lie conformal *** this paper,we investigate the extending structures problem for Gel'fand-Dorfman bialgebras,which is equivalent to some extending structures problem of quadratic Lie conformal ***,given a Gel'fand-Dorfman bialgebra(A,o,[.,.]),this problem is how to describe and classify all Gel'fand-Dorfman bialgebra structures on a vector space E(A⊂E)such that(A,o,[.,.])is a subalgebra of E up to an isomorphism whose restriction on A is the identity *** by the theories of extending structures for Lie algebras and Novikov algebras,we construct an object gH2(V,A)to answer the extending structures problem by introducing the notion of a unified product for Gel'fand-Dorfman bialgebras,where V is a complement of A in *** particular,we investigate the special case when dim(V)=1 in detail.

关键词： Gel'fand-Dorfman bialgebra Lie conformal algebra Extending structures problem Novikovalgebra

Facile synthesis of transition metal complexes with five coplanar metal-carbon σ-bonds

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National Science Review 2024年第4期11卷 11-12页

作者： Guocheng jia Department of Chemistry Hong Kong University of Science and Technology

Carbon is one of the most important elements in chemistry, forming a vast number of organic compounds. It can also form a variety of organometallic compounds with a metal–carbon bond. Metal alkyl, carbene and carbyne complexes are classical examples of organometallic compounds. A particularly interesting class of organometallic compounds are all-carbon metallacycles formed by combination of a metal and a hydrocarbon chain serving as a chelating agent with only carbon atoms as the donor atoms. Many interesting monocyclic metallacycles made of a chelating bidentate hydrocarbon chain are known, for example, metallacyclobutadienes, metallacyclopentadienes and metallabenzenes[1].

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