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Understanding recurrent pregnancy loss:recent advances on its etiology,clinical diagnosis,and management

Medical Review 2022年第6期2卷 570-589页

作者： Chunwei Cao Shiyu Bai Jing Zhang Xiaoyue Sun anming meng Hui Chen Medical Research Center Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510000Guangdong ProvinceChina Guangzhou Laboratory Guangzhou 510000Guangdong ProvinceChina Center for Reproductive Genetics and Reproductive Medicine Sun Yat-Sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510000Guangdong ProvinceChina Reproductive Medicine Center Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Medical Research Center Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangdong ProvinceChina Center for Reproductive Genetics and Reproductive Medicine Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhouGuangdong ProvinceChina Guangzhou laboratory GuangzhouGuangdong ProvinceChina Reproductive Medicine Center Sun Yat-sen Memorial HospitalSun Yat-sen University 107 YanjiangGuangzhou 510000Guangdong ProvinceChina

Recurrent pregnancy loss(RPL)has become an important reproductive health issue worldwide.RPL affects about 2%–3%of reproductive-aged women,and makes serious threats to women’s physical and mental health.However,the etiology of approximately 50%of RPL cases remains unknown(unexplained RPL),which poses a big challenge for clinical management of these patients.RPL has been widely regarded as a complex disease where its etiology has been attributed to numerous factors.Heretofore,various risk factors for RPL have been identified,such as maternal ages,genetic factors,anatomical structural abnormalities,endocrine dysfunction,prethrombotic state,immunological factors,and infection.More importantly,development and applications of next generation sequencing technology have significantly expanded opportunities to discover chromosomal aberrations and single gene variants responsible for RPL,which provides new insight into its pathogenic mechanisms.Furthermore,based upon patients’diagnostic evaluation and etiologic diagnosis,specific therapeutic recommendations have been established.This review will highlight current understanding and recent advances on RPL,with a special focus on the immunological and genetic etiologies,clinical diagnosis and therapeutic management.

关键词： etiologic diagnosis genetic etiology next generation sequencing recurrent pregnancy loss therapeutic recommendations

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The second polar body contributes to the fate asymmetry in the mouse embryo

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National Science Review 2022年第7期9卷 32-42页

作者： Hongbin Jin Yang Han Huasong Wang J.Xiao He Li Weimin Shen Lin Zhang Luxi Chen Shunji Jia Ping Yuan Hui Chen anming meng Laboratory of Molecular Developmental Biology State Key Laboratory of Membrane BiologyTsinghua-Peking Center for Life Sciences School of Life SciencesTsinghua University Center for Reproductive Genetics and Reproductive Medicine Sun Yat-sen Memorial HospitalSun Yat-sen University Guangzhou Laboratory

The polar bodies(PBs) are extruded microcells during oocyte meiosis and generally regarded as inessentials for embryonic development.Therefore,PBs have been widelyused as important materials for pre-implantation genetic diagnosis in human.Here we report that the second PB(PB2) in the mouse zygote may play roles in cell-fate specification and post-implantation development.A subset of mRNAs encoding pluripotency-related factors are enriched in PB2.Nascent proteins maybe synthesized in PB2 after fertilization and transport from PB2 to the zygote before the two-cell stage.The PB2-attached blastomere(pbB) at the two-cell stage,compared to the other blastomere(npbB),likely contributes more descendants to the inner cell mass(ICM) lineage in the blastocyst.Removal of PB2 from the zygote or transient blockage of material exchange between PB2 and the zygote by nocodazole treatment appears to cause a loss of the ICM fate bias of pbB.PB2 removal or nocodazole treatment also results in abnormal post-implantation development.Injection of PB2 lysate into pbB of PB2-removed two-cell-stage embryos may reset the cell-fate preference and rescue post-implantation development.Our data collectively suggest that PB2 would demarcate the earliest cell-fate asymmetry of the mouse zygote and be required for post-implantation development.

关键词： mouse embryo polar body cell fate asymmetry

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The genetic program Of oocytes can be modified in vivo in the zebrafish ovary

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Journal of Molecular Cell Biology 2018年第6期10卷 479-493页

作者： Xiaotong Wu Weimin Shen Bingjie Zhang anming meng Laboratory of Molecular Developmental Biology State Key Laboratory of Membrane BiologyTsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Center for Stem Cell Biology and Regenerative Medicine MOE Key Laboratory of BioinformaticsTsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China

Oocytes,the irreplaceable gametes for generating a new organism,are matured in the ovary of living female animals.It is unknown whether any genetic manipulations can be applied to immature oocytes inside the living ovaries.As a proof-of-concept, we here demonstrate genetic amendments of zebrafish immature oocytes within the ovary.Oocyte microinjection in situ (OMIS) stimulates tissue repair responses,but some of the microinjected immature oocytes are matured,ovulated and fertilizable.By OMIS-mediated Cas9 approach,ntla and gata5 loci of oocytes arrested at prophase I of meiosis are successfully edited before fertilization.Through OMIS,high efficiency of biaUeUc mutations in single or multiple loci using Cas9/gRNAs allows immediate manifestation of mutant phenotypes in Fo embryos and multiple transgenes can co-express the reporters in Fo embryos with patterns similar to germline transgenic embryos.Furthermore,maternal knockdown of dnmt1 by antisense morphoino via OMIS results in a dramatic decrease of global DNA methylation level at the dome stage and causes embryonic lethalityprior to segmentation period.Therefore,OMIS opens a door to efficiently modify the genome and provides a possibility to re repair genetically abnormal oocytes in situ.

关键词： oocytes microinjection gene editing transgenesis morpholino zebrafish

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Ecological problems and ecological restoration zoning of the Aral Sea

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Journal of Arid Land 2024年第3期16卷 315-330页

作者： BAO anming YU Tao XU Wenqiang LEI Jiaqiang JIAPAER Guli CHEN Xi Tojibaev KOMILJON Shomurodov KHABIBULLO Xabibullaev B SAGIDULLAEVICH Idirisov KAMALATDIN State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011China Key Laboratory of GIS&RS Application Xinjiang Uygur Autonomous RegionUrumqi 830011China University of Chinese Academy of Sciences Beijing 100049China CAS Research Center for Ecology and Environment of Central Asia Urumqi 830011China Institute of Botany of the Academy of Sciences of the Republic of Uzbekistan Tashkent 100125Uzbekistan International Innovation Center for Aral Sea Basin under the President of the Republic of Uzbekistan Nukus 230100Uzbekistan

The Aral Sea was the fourth largest lake in the world but it has shrunk dramatically as a result of irrational human activities, triggering the "Aral Sea ecological crisis". The ecological problems of the Aral Sea have attracted widespread attention, and the alleviation of the Aral Sea ecological crisis has reached a consensus among the five Central Asian countries(Kazakhstan, Uzbekistan, Tajikistan, Kyrgyzstan, and Turkmenistan). In the past decades, many ecological management measures have been implemented for the ecological restoration of the Aral Sea. However, due to the lack of regional planning and zoning, the results are not ideal. In this study, we mapped the ecological zoning of the Aral Sea from the perspective of ecological restoration based on soil type, soil salinity, surface water, groundwater table, Normalized Difference Vegetation Index(NDVI), land cover, and aerosol optical depth(AOD) data. Soil salinization and salt dust are the most prominent ecological problems in the Aral Sea. We divided the Aral Sea into 7 first-level ecological restoration subregions(North Aral Sea catchment area in the downstream of the Syr Darya River(Subregion Ⅰ);artificial flood overflow area in the downstream of the Aral Sea(Subregion Ⅱ);physical/chemical remediation area of the salt dust source area in the eastern part of the South Aral Sea(Subregion Ⅲ);physical/chemical remediation area of severe salinization in the central part of the South Aral Sea(Subregion Ⅳ);existing water surface and potential restoration area of the South Aral Sea(Subregion Ⅴ);Aral Sea vegetation natural recovery area(Subregion Ⅵ);and vegetation planting area with slight salinization in the South Aral Sea(Subregion Ⅶ)) and 14 second-level ecological restoration subregions according to the ecological zoning principles. Implementable measures are proposed for each ecological restoration subregion. For Subregion Ⅰ and Subregion Ⅱ with lower elevations, artificial flooding should be carried out to restore the surface of the Aral Sea. Subregion Ⅲ a

关键词： ecological restoration zoning salt and dust storms soil salinization ecological crisis Aral Sea Central Asia

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A zebrafish gene trap line expresses GFP recapturing expression pattern of foxj1b

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Journal of Genetics and Genomics 2009年第10期36卷 581-589页

作者： Tian Tian Long Zhao Xinyi Zhao Min Zhang anming meng Protein Science Laboratory of Ministry of Education Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China State Key Laboratory of Biomembrane and Membrane Engineering Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

Foxj1 has been found to play an important role in cilia formation and function in vertebrates. The zebrafish or Xenopus genome expresses two Foxjl genes, foxjlalFoxJ1 and foxjlb/FoxJ1.2. In this study, we have generated a zebrafish transgenic line T2BGSZIO by Tol2 transposon-based gene trapping approach. T2BGSZ10 transgenic fish carry an insertion of the transposon genome into the first intron of thefoxj1b locus. This insertion results in GFP expression in the forebrain, otic vesicles, floorplate, pronephric ducts and other domains during embryogenesis, which recaptures the expression pattern offoxj1b. Although normal expression offoxj1b is dramatically reduced, T2BGSZ10 homozygous embryos develop normally and grow to adulthood without detectable defects, which may be due to the incomplete interruption of foxjlb expression. Nevertheless, this transgenic line may serve as a useful model for dynamic observation of GFP-labeled tissues and organs and for isolation of GFP-labeled cells.

关键词： zebrafish transposon embryo transgenic foxj1

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The Tiny Zebrafish Keep Swimming Fast in the Developmental Biology Pond

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Journal of Genetics and Genomics 2012年第9期39卷 419-420页

作者： Jinrong Peng Jian Zhang anming meng College of Animal Sciences Zhejiang UniversityHangzhou 310058China Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China School of Life Sciences Tsinghua UniversityBeijing 100084China

In 1996,the journal Development published a special issue on zebrafish solely focusing on characterization of dozens of phenotypic mutants chosen from hundreds of mutants identified through chemical（ENU）mutagenesis by two zebrafish groups in Tubingen and Boston.This milestone formally catapulted zebrafish to a league of genetically tractable model

关键词： life sciences

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Preface to the special topic on gene editing towards translation

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National Science Open 2023年第5期2卷 3-4页

作者： Jinsong Li anming meng State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China Laboratory of Molecular Developmental Biology State Key Laboratory of Membrane BiologyTsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China

The first draft of the human genome,published in 2001,has had a broad effect on biomedical studies and applications.In the past two decades,the human genome project(HGP)has dramatically promoted our understanding of how genomic sequences work during development,diseases and evolution.Meanwhile,genome-editing technologies have been rapidly developed to precisely and efficiently rewrite DNA sequences of different species,especially in 2012 as the development of a revolutionary genome editing method based on clustered regularly interspaced short palindromic repeat(CRISPR)and CRISPR-associated(Cas)proteins.

关键词： evolution. sequences editing

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Induction of cranial and posterior trunk neural crest by exogenous retinoic acid in zebrafish

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Chinese Science Bulletin 2002年第13期47卷 1105-1108,1146页

作者： Ming Li Ying Su anming meng Tsing Hua Univ Dept Biol Sci & Biotechnol Prot Sci Lab MOE Beijing 100084 Peoples R China

Retinoic acid (RA) plays an important role in development of vertebrate embryos. We demonstrate impacts of exogenous RA on the formation of neural crest cells in zebrafish using specific neural crest markers sox9b and crestin. Treatment with all-trans RA at 10-7 mmol/L at 50% epiboly induces sox9b expression in the forebrain and crestin expression in the forebrain and midbrain, resulting in significant increase of pigment cells in the head derived from the cranial neural crest. In addition, RA treatment induces expression of sox9b and crestin in the caudal marginal cells of the neuroectoderm during early segmentation. Earlier commitment of these cells to the neural crest fate in the posterior margins leads to abnormal development of the posterior body, probably by preventing mingling of ventral derived and dorsal-derived cells during the formation of the tailbud.

关键词： zebrafish retinoic acid neural crest sox9b crestin.

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prpf4 is essential for cell survival and posterior lateral line primordium migration in zebrafish

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Journal of Genetics and Genomics 2018年第8期45卷 443-453页

作者： Yixia Wang Yanchao Han Pengfei Xu Shihui Ding Guangyuan Li Hongbin Jin Yaping meng anming meng Shunji Jia State Key Laboratory of Membrane Biology Tsinghua-Peking Center for Life Sciences School of Life Sciences Tsinghua University

Prpf4 （pre-mRNA processing factor 4）, a key component of spliceosome, plays critical roles in pre-mRNA splicing and its mutations result in retinitis pigmentosa due to photoreceptor defects. In this study, we characterized a zebrafish prpf4t243 mutant harboring a Tol2 transposon-based gene trap cassette in the third intron of the prpf4 gene. Cells in the brain and spinal cord gradually undergo p53-dependent apoptosis after 28 hpf in prpf4t243 mutants, suggesting that a widespread function of prpf4 in neural cell survival. In addition, prpf4 is essential for survival of posterior lateral line primordial （pLLP） cells, prpf4 deficiency perturbs Fgf, Wnt/β-catenin and chemokine signaling pathways and impairs pLLP migration. RNA-Seq analysis suggests that prpf4 deficiency may impair spliceosome assembly, leading to compensatory upregulation of core spliceosomal genes and alteration of pre-mRNA splicing. Taken together, our studies uncover an essential role of prpf4 in pre-mRNA splicing, cell survival and pLLP migration.

关键词： prpf4 Splicing Apoptosisp LLP Zebrafish

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The rising zebrafish research in China：Meeting report of the 3rd Chinese Zebrafish Principal Investigator Meeting ＆ the Inaugural Meeting of China Zebrafish Society

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Journal of Genetics and Genomics 2016年第10期43卷 617-620页

作者： Dongyuan Ma Yuanyuan Xue Yifan Zhang Yonghua Sun anming meng Feng Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Freshwater Ecology and Biotechnology China Zebrafish Resource CenterInstitute of HydrobiologyChinese Academy of SciencesWuhan 430072China State Key Laboratory of Membrane Biology Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China

The zebrafish has become a very important animal model,not only for developmental biology and genetics,but also for disease modeling and drug discovery.Since the year of 2000,zebrafish research has been rapidly growing in China,and the laboratories using zebrafish as the main subject have been increasing dramatically（Xie et al.,2015）.For example,the number of participants who attended the Chinese Zebrafish Principle Investigator （PI） Meeting series is 80 in 2012, 150 in 2014, and over 200 this year.

关键词： the Inaugural Meeting of China Zebrafish Society The rising zebrafish research in China

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