The genetic program Of oocytes can be modified in vivo in the zebrafish ovary
作者机构：Laboratory of Molecular Developmental BiologyState Key Laboratory of Membrane BiologyTsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Center for Stem Cell Biology and Regenerative MedicineMOE Key Laboratory of BioinformaticsTsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China
出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)
年 卷 期：2018年第10卷第6期
页 面：479-493页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 09[农学]
基 金：the National Natural Science Foundation of China (31330052 and 31590832)
主 题：oocytes microinjection gene editing transgenesis morpholino zebrafish
摘 要：Oocytes,the irreplaceable gametes for generating a new organism,are matured in the ovary of living female *** is unknown whether any genetic manipulations can be applied to immature oocytes inside the living *** a proof-of-concept, we here demonstrate genetic amendments of zebrafish immature oocytes within the *** microinjection in situ (OMIS) stimulates tissue repair responses,but some of the microinjected immature oocytes are matured,ovulated and *** OMIS-mediated Cas9 approach,ntla and gata5 loci of oocytes arrested at prophase I of meiosis are successfully edited before *** OMIS,high efficiency of biaUeUc mutations in single or multiple loci using Cas9/gRNAs allows immediate manifestation of mutant phenotypes in Fo embryos and multiple transgenes can co-express the reporters in Fo embryos with patterns similar to germline transgenic ***,maternal knockdown of dnmt1 by antisense morphoino via OMIS results in a dramatic decrease of global DNA methylation level at the dome stage and causes embryonic lethalityprior to segmentation ***,OMIS opens a door to efficiently modify the genome and provides a possibility to re repair genetically abnormal oocytes in situ.
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