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Progress in Natural Science:Materials International 2015年第2期25卷 122-130页

作者： Valentina Mitran Madalina Georgiana Albu Eugeniu Vasile anisoara cimpean Marieta Costache University of Bucharest Faculty of Biology Department of Biochemistry and Molecular Biology INCDTP – Division Leather and Footwear Research Institute Collagen Department 3. University Politehnica of Bucharest Advanced Polymer Materials Group

This paper aims at demonstrating the biocompatibility of recently developed 3D hydrogel scaffolds containing the same amount of collagen(COLL) and variable concentrations of sericin(SS) in order to find the most suitable formula for adipose tissue engineering(ATE) *** scaffolds were obtained by COLL crosslinking with glutaraldehyde followed by freeze-drying and, subsequently, seeded with 3T3-L1 preadipocytes. Scanning electron microscopy studies revealed the scaffolds’ architecture and cellular colonization. Also, in vitro biocompatibility of the developed scaffolds was evaluated by LDH and MTT assays and Live/Dead analysis of 3T3-L1 preadipocyte populating these 3D *** best results in terms of cell survival and proliferation status were obtained in the case of the hybrid COLL scaffold containing 40% SS(COLL–SS4). Furthermore, the biological performance of the analyzed COLL-based hydrogels at 5- and 8- days post-seeding was found to decrease as follows: COLL–SS4 4 COLL–SS2 4 COLL 4 COLL–SS6. Consequently, our study highlights that hybrid scaffolds obtained by the addition of variable concentrations of SS to a constant COLL composition positively influences the behavior of 3T3-L1 cells with the exception of the COLL–SS6 matrix(60% SS). Altogether, the data obtained recommend SS as a component of COLL-based hydrogels providing them with features that may be useful in ATE applications.

关键词： Sericin–collagen scaffold Preadipocyte Proliferation Morphology Tissue engineering

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In vitro study of human endothelial progenitor cells behaviour on nitrided Ni-free Ti–27Nb alloy

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Progress in Natural Science:Materials International 2019年第4期29卷 466-471页

作者： Raluca Ion Yvan Bédouin Thierry Gloriant Gladiola Andruseac Doina-Margareta Gordin anisoara cimpean University of Bucharest Department of Biochemistry and Molecular Biology Univ Rennes INSA Rennes CNRS ISCR (Institut des Sciences Chimiques de Rennes) CHU de Rennes P?le d'Odontologie et de Chirurgie Buccale Department of Biomedical Science Faculty of Medical Bioengineering “Grigore T.Popa” University of Medicine and Pharmacy

A superelastic Ni-free Ti-27 Nb alloy has been synthesized and gas nitrided at high temperature to investigate its suitability for vascular implant *** cellular responses of human endothelial progenitor cells(EPCs)to both bare and nitrided Ti-27 Nb alloy have been analyzed using Live/Dead staining,MTT assay,fluorescence microscopy and ELISA technique,as well as NiTi alloy for ***/Dead staining and MTT assay were performed to assess the cellular viability and proliferation,while fluorescence microscopy was used to analyze cell adhesion,cell morphology,and the expression of endothelial cell markers(VE-cadherin and von Willebrand factor).Secretion of the pro-inflammatory chemokine MCP(monocyte chemoattractant protein)-1 by the cells grown in contact with the analyzed materials was further verified using the enzyme-linked immunosorbent *** results obtained revealed that adhesion,spreading,viability,proliferation rate and phenotypic markers expression of EPCs were similar on the surfaces of Ti-27 Nb and NiTi *** exposed to nitrided Ti-27 Nb surface exhibited significantly decreased inflammatory response,which may be beneficial for reducing in-stent restenosis incidence.

关键词： Titanium alloy Nitriding Endothelial progenitor cells Biocompatibility Inflammatory response

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