In vitro study of human endothelial progenitor cells behaviour on nitrided Ni-free Ti–27Nb alloy

作     者：Raluca Ion Yvan Bédouin Thierry Gloriant Gladiola Andruseac Doina-Margareta Gordin Anisoara Cimpean 

作者机构：University of Bucharest Department of Biochemistry and Molecular Biology Univ Rennes INSA Rennes CNRS ISCR (Institut des Sciences Chimiques de Rennes) CHU de Rennes P?le d'Odontologie et de Chirurgie Buccale Department of Biomedical Science Faculty of Medical Bioengineering “Grigore T.Popa” University of Medicine and Pharmacy 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2019年第29卷第4期

页      面：466-471页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：financially supported by the Romanian Ministry of National Education  CNCS-UEFISCDI (project PN-II-ID-20-RO-FR2014) 

主　　题：Titanium alloy Nitriding Endothelial progenitor cells Biocompatibility Inflammatory response 

摘      要：A superelastic Ni-free Ti-27 Nb alloy has been synthesized and gas nitrided at high temperature to investigate its suitability for vascular implant *** cellular responses of human endothelial progenitor cells(EPCs)to both bare and nitrided Ti-27 Nb alloy have been analyzed using Live/Dead staining,MTT assay,fluorescence microscopy and ELISA technique,as well as NiTi alloy for ***/Dead staining and MTT assay were performed to assess the cellular viability and proliferation,while fluorescence microscopy was used to analyze cell adhesion,cell morphology,and the expression of endothelial cell markers(VE-cadherin and von Willebrand factor).Secretion of the pro-inflammatory chemokine MCP(monocyte chemoattractant protein)-1 by the cells grown in contact with the analyzed materials was further verified using the enzyme-linked immunosorbent *** results obtained revealed that adhesion,spreading,viability,proliferation rate and phenotypic markers expression of EPCs were similar on the surfaces of Ti-27 Nb and NiTi *** exposed to nitrided Ti-27 Nb surface exhibited significantly decreased inflammatory response,which may be beneficial for reducing in-stent restenosis incidence.