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The application of digital holography for accurate three-dimensional localisation of mosquito-bednet interaction

Light(Advanced Manufacturing) 2022年第3期3卷 13-27页

作者： Matthew L Hall Katherine Gleave angela hughes Philip J McCall Catherine E Towers David P Towers Optical Engineering Group School of EngineeringUniversity of WarwickCoventryCV47ALUK Vector Biology Department Liverpool School of Tropical MedicineLiverpoolL35QAUK

Understanding mosquito interaction with long-lasting insecticidal bednets is crucial in the development of more effective intervention methods to protect humans from malaria *** such,a 240×240×1000 mm laboratory setup for the in-line recording of digital holograms and subsequent in-focus reconstruction and 3D localisation of mosquitoes is *** bednet background removal methods are used to accurately localise a mosquito obscured by a bednet in 3D *** and physical data demonstrate that this method is suitable for mosquitoes positioned 3−1000 mm behind a bednet.A novel post-processing technique,involving a cascade-correlation of a Tamura of Intensity focus metric extracted from digitally reconstructed scenes,accurately localises mosquitoes positioned 35−100 mm behind a bednet from a single digital *** result of this study is a scalable digital holographic methodology to examine mosquito-bednet interaction in 3D at a level of accuracy previously only seen in 2D imaging of mosquitoes in a much smaller volume.

关键词： Digital Holography Metrology Entomology

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GARLIC AND HEALTH: CULTIVATION AND SULFUR BIOCHEMISTRY OF GARLIC

GARLIC AND HEALTH: CULTIVATION AND SULFUR BIOCHEMISTRY OF GA...

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4～(th) International Ishs Symposium on Edible Alliaceae(ISEA)

作者： Brian Thomas Laurence Trueman Linda Brown Brian Smith angela Tregova Jill hughes Jonothan Milne Meriel Jones Brian Tomsett Mark Wilkinson Hamish Collin Odile Huchette Remi Kahane Jacques Auger Ingrid Arnault Xabier Barandiaran J.Martin Sanchez Veronique C Horticulture Research International Wellesbourne Warwick CV35 9EF Horticulture Research International Wellesbourne Warwick CV35 9EF Horticulture Research International Wellesbourne Warwick CV35 9EF Horticulture Research International Wellesbourne Warwick CV35 9EF University of Liverpool School of Biological Sciences The Biosciences Building Liverpool L69 7ZB University of Liverpool School of Biological Sciences The Biosciences Building Liverpool L69 7ZB University of Liverpool School of Biological Sciences The Biosciences Building Liverpool L69 7ZB University of Liverpool School of Biological Sciences The Biosciences Building Liverpool L69 7ZB University of Liverpool School of Biological Sciences The Biosciences Building Liverpool L69 7ZB University of Liverpool School of Biological Sciences The Biosciences Building Liverpool L69 7ZB University of Liverpool School of Biological Sciences The Biosciences Building Liverpool L69 7ZB DIJON CEREALES/COOPD’OR R＆D Department INRA21110 BretenieresFrance （RN5 21130 Villers-Les-PotsFrance） CIRAD-FLHOR 34398 Montpellier Cedex 5 France Universite de F. Rabelais IRBI Faculte des Sciences et Techniques 37200 Tours France Universite de F. Rabelais IRBI Faculte des Sciences et Techniques 37200 Tours France Universidad de Cordoba San Alberto Magna14004 Cordoba Spain Universidad de Cordoba San Alberto Magna14004 Cordoba Spain INRA Pathologie Vegetale 84143 Montfavet France

＜正＞ Research groups in France, Spain and the UK have collaborated to obtain a better picture of the environmental factors that affect the flavour levels in garlic cloves, the movement of sulfur in the plant as it develops and the biosynthetic pathways of the flavour precursors. The major flavour precursor of garlic, alliin, is the source of its characteristic flavour and is also

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Retinoic acid signaling acts as a rheostat to balance Treg function

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Cellular & Molecular Immunology 2022年第7期19卷 820-833页

作者： Govindarajan Thangavelu Gabriela Andrejeva Sara Bolivar-Wagers Sujeong Jin Michael C.Zaiken Michael Loschi Ethan G.Aguilar Scott N.Furlan Chrysothemis C.Brown Yu-Chi Lee Cameron McDonald Hyman Colby J.Feser angela Panoskaltsis-Mortari Keli L.Hippen Kelli P.MacDonald William J.Murphy Ivan Maillard Geoffrey R.Hill David H.Munn Robert Zeiser Leslie S.Kean Jeffrey C.Rathmell Hongbo Chi Randolph J.Noelle Bruce R.Blazar Department of Pediatrics Center for ImmunologyUniversity of MinnesotaMinneapolisMNUSA Center for Immunobiology Vanderbilt University Medical CenterNashville TNUSA Department of Pediatrics University of WashingtonSeattleWAUSA Fred Hutchinson Cancer Research Center SeattleWAUSA Howard hughes Medical Institute Immunology Program and Ludwig CenterSloan Kettering InstituteMemorial Sloan Kettering Cancer CenterNew YorkNYUSA Department of Microbiology and Immunology.Geisel School of Medicine at Dartmouth Norris Cotton Cancer CenterLebanonUSA Department of Immunology Queensland Institute of Medical Research(QIMR)Berghofer Medical Research Institute and School of MedicineUniversity of QueenslandBrisbaneQLDAustralia Departmentof Dermatology School of MedicineUniversity of California DavisSacramentoCAUSA Division of Hematology/Oncology Department of MedicineUniversity of Pennsylvania Perelman School of MedicinePhiladelphiaPAUSA Georgia Cancer Center Augusta UniversityAugustaGAUSA Department of Haematology Oncology and Stem Cell TransplantationFaculty of MedicineFreiburg University Medical CentreFreiburgGermany Boston Children's Hospital and the Dana-Farber Cancer Institute BostonMAUSA Department of Immunology St.Jude Children's ResearchHospitalMemphisTNUSA

Regulatory T cells(Tregs)promote immune homeostasis by maintaining self-tolerance and regulating inflammatory *** certain inflammatory conditions,Tregs can lose their lineage stability and *** studies have reported that ex vivo exposure to retinoic acid(RA)enhances Treg function and ***,it is unknown how RA receptor signaling in Tregs influences these processes in ***,we employed mouse models in which RA signaling is silenced by the expression of the dominant negative receptor(DN)RARαin all T *** the fact that DNRARαconventional T cells are hypofunctional,Tregs had increased CD25 expression,STAT5 pathway activation,mTORC1 signaling and supersuppressor ***,DNRARαTregs had increased inhibitory molecule expression,amino acid transporter expression,and metabolic fitness and decreased antiapoptotic *** function was observed when wild-type mice were treated with a pharmacologic pan-RAR ***,Treg-specific expression of DNRARαresulted in distinct phenotypes,such that a single allele of DNRARαin Tregs heightened their suppressive function,and biallelic expression led to loss of suppression and *** loss of Treg function was not cell intrinsic,as Tregs that developed in a noninflammatory milieu in chimeric mice reconstituted with DNRARαand wild-type bone marrow maintained the enhanced suppressive *** mapping suggested that maintaining Treg stability in an inflammatory milieu requires RA *** findings indicate that RA signaling acts as a rheostat to balance Treg function in inflammatory and noninflammatory conditions in a dose-dependent manner.

关键词： Retinoic acid Tregulatory cells Autoimmunity Metabolism mTORC1 STAT5

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