The application of digital holography for accurate three-dimensional localisation of mosquito-bednet interaction

作     者：Matthew L Hall Katherine Gleave Angela Hughes Philip J McCall Catherine E Towers David P Towers 

作者机构：Optical Engineering GroupSchool of EngineeringUniversity of WarwickCoventryCV47ALUK Vector Biology DepartmentLiverpool School of Tropical MedicineLiverpoolL35QAUK 

出 版 物：《Light(Advanced Manufacturing)》 (光（先进制造）（英文）)

年 卷 期：2022年第3卷第3期

页      面：13-27页

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：This work was supported by the Engineering and Physical Sciences Research Council under Grant Number EP/N509796/1.A UK Medical Research Council award(MRC:MR/M011941/1)also contributed to this work 

主　　题：Digital Holography Metrology Entomology 

摘      要：Understanding mosquito interaction with long-lasting insecticidal bednets is crucial in the development of more effective intervention methods to protect humans from malaria *** such,a 240×240×1000 mm laboratory setup for the in-line recording of digital holograms and subsequent in-focus reconstruction and 3D localisation of mosquitoes is *** bednet background removal methods are used to accurately localise a mosquito obscured by a bednet in 3D *** and physical data demonstrate that this method is suitable for mosquitoes positioned 3−1000 mm behind a bednet.A novel post-processing technique,involving a cascade-correlation of a Tamura of Intensity focus metric extracted from digitally reconstructed scenes,accurately localises mosquitoes positioned 35−100 mm behind a bednet from a single digital *** result of this study is a scalable digital holographic methodology to examine mosquito-bednet interaction in 3D at a level of accuracy previously only seen in 2D imaging of mosquitoes in a much smaller volume.