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proteomic analysis of F_(1)hybrids and intermediate variants in a Littorina saxatilis hybrid zone

Current Zoology 2022年第3期68卷 351-359页

作者： angel p.diz Mónica R.ROMERO Juan GALINDO María SAURA David O.F.SKIBINSKI Emilio ROLÁN-ALVAREZ Centro de Investigación Mariña(CIM-UVIGO) Universidade de VigoVigo 36310Spain Departamento de Mejora Genética Animal INIAMadrid 28040Spain Institute of Life Science Swansea University Medical SchoolSwansea SA28PPUK

proteomic analysis was carried out on the Crab(upper-shore)and Wave(lower-shore)ecotypes of Littorina saxatilis from a hybrid zone at Silleiro Cape,*** profiles of individual snails were *** expression in F_(1)hybrid snails bred in the laboratory and snails with intermediate shell phenotypes collected from the mid-shore were compared with Crab and Wave ecotypes using analytical approaches used to study *** analysis over many protein spots showed that the F_(1)snails are distinct from both ecotypes but closer to the Wave *** intermediate snails are highly variable,some closer to the Crab and others to the Wave *** on a protein by protein basis,some proteins are significantly closer in expression to the Crab and others to the Wave ecotype for both F_(1)and intermediate ***,a significant majority of proteins were closer in expression to the Wave ecotype for the F_(1),consistent with the multivariate *** such significant majority toward either the Crab or Wave ecotype was observed for the intermediate *** closer similarity of F_(1)and Wave ecotype expression patterns could be the result of similar selective pressures in the similar mid-shore and low-shore *** a significantly larger number of proteins,intermediate snails were closer in expression to the ecotype having the lower expression,for both Crab and Wave *** is somewhat unexpected as lower expression might be expected to be an indication of impairment of function and lower *** analysis could be important for the identification of candidate proteins useful for gaining improved understanding of adaptation and barriers to gene flow in hybrid zones.

关键词： F_(1)hybrids gene expression marine invertebrates molecular phenotype reproductive isolation speciation

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Limited proteomic response in the marine snail Melarhaphe neritoides after long-term emersion

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Current Zoology 2017年第5期63卷 487-493页

作者： angel R diz Margarita ALVAREZ-RODRiGUEZ Monica R. ROMERO Emilio FOLAN-ALVAREZ Juan GALINDO Department of Biochemistry Genetics and Immunology Faculty of Biology University of Vigo Vigo 36310 Spain Toralla Marine Science Station (ECIMAT) Universidad de Vigo Vigo 36331 Spain Institute of Marine Research (IIM) CSIC Vigo 36208 Spain

Rocky intertidal organisms are commonly exposed to environmental gradients, promoting adapta-tions to these conditions. Emersion time varies along the intertidal range and in the supralittoralzone is frequently larger than a single tidal cycle, even lasting for weeks. The planktonic-dispersinggastropod Melarhaphe neritoides is a common species of the high shore, adapted to reduce waterloss in order to survive during long-term emersion. In this study, we investigated the molecular re-sponse, at the proteome level, of M. neritoides collected in high-shore tide pools to a series ofemersion periods, from 8 to 24 days, in laboratory conditions. We compared this response to indi-viduals maintained submerged during this period, because this was their original habitat. We alsoincluded a reversion treatment in the study, in which emersed individuals were returned to the sub-merged conditions. Although we detected an increase in overall protein concentration with longeremersion periods, contrary to general expectation, the two dimensional electrophoresis （2DE）-based proteomic analysis did not show significant differences between the treatments at the levelof individual protein spots, even after an emersion period of 24 days. Our results suggest that themetabolism remains unaltered independent of the treatment carried out or the changes are verysubtle and therefore difficult to detect with our experimental design. We conclude that M. neri-toides could be equally adapted to emersion and submersion without drastic physiologicalchanges.

关键词： daptation, 2DE proteomics, environmental gradient, intertidal zone, metabolic rate depression.

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Can sexual selection and disassortative mating contribute to the maintenance of a shell color polymorphism in an inter- tidal marine snail？

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Current Zoology 2012年第3期58卷 463-474页

作者： Emilio ROLALVAREZ Maria SAURA angel E diz Marfa JOSE RIVAS MargaALVAREZ Beatriz CORTES Alicia de COO Daniel ESTEVEZ Laura IGLESIAS Departamento de Bioqufmica Genetica e Inmunologfa Facultad de Biologfa Universidad de Vigo Campus de Vigo 36310 VigoSpain

Littorina fabalis is an intertidal snail commonly living on the brown algae Fucus vesiculosus and showing frequent shell-color polymorphisms in the wild. The evolutionary mechanism underlying this polymorphism is currently unknown. Shell color variation was studied in mated and non-mated specimens of this species from different microareas in one locality from NW Spain, in order to estimate sexual selection and assortative mating that may （still） be operating in this population. The analyses across microareas allowed us to investigate frequency-dependent selection and assortative mating components, mechanisms that could maintain the polymorphism. The presence of shell scars caused by crab attacks, an environmental variable not related with sexual selection or assortative mating, was used as experimental control. This study provides new evidence of significant disas- sortative mating and some degree of sexual selection against some shell colors, supporting the results found 21 years ago in a similar study, i.e. in the same species and locality. The similarity of these estimates during the studied period suggests that this experimental approach is consistent and valid to be extended to other populations and organisms. In addition, sexual selection and assortative mating estimates did not change across microareas differing in shell color frequencies, suggesting than the polymor- phism can not be maintained by a frequency-dependent （sexual selection-based） mechanism. Our main hypothesis is that negative assortative mating could contribute to the maintenance of the polymorphism, perhaps by males showing distinct female color preferences when searching for mates [Current Zoology 58 （3）： 463-474, 2012].

关键词： Fitness estimate Mate choice Mate propensity Negative assortative mating Sexual selection Frequency-dependentselection

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