Limited proteomic response in the marine snail Melarhaphe neritoides after long-term emersion
作者机构：Department of Biochemistry Genetics and Immunology Faculty of Biology University of Vigo Vigo 36310 Spain Toralla Marine Science Station (ECIMAT) Universidad de Vigo Vigo 36331 Spain Institute of Marine Research (IIM) CSIC Vigo 36208 Spain
出 版 物：《Current Zoology》 (动物学报（英文版）)
年 卷 期：2017年第63卷第5期
页 面：487-493页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
基 金：partially funded by MEYC Xunta de Galicia and Fondos FEDER: "Unha maneira de facer Europa" (Grupos con Potencial Crecimiento) supported by a postdoctoral fellowship from Xunta de Galicia (Modalidade B) M Alvarez- Rodriguez by an FPU fellowship (Ministerio de Educacion, Cultura y Deporte) MR Romero by a predoctoral fellowship from Xunta de Galicia (Campus do Mar)
主 题：daptation, 2DE proteomics, environmental gradient, intertidal zone, metabolic rate depression.
摘 要：Rocky intertidal organisms are commonly exposed to environmental gradients, promoting adapta-tions to these conditions. Emersion time varies along the intertidal range and in the supralittoralzone is frequently larger than a single tidal cycle, even lasting for weeks. The planktonic-dispersinggastropod Melarhaphe neritoides is a common species of the high shore, adapted to reduce waterloss in order to survive during long-term emersion. In this study, we investigated the molecular re-sponse, at the proteome level, of M. neritoides collected in high-shore tide pools to a series ofemersion periods, from 8 to 24 days, in laboratory conditions. We compared this response to indi-viduals maintained submerged during this period, because this was their original habitat. We alsoincluded a reversion treatment in the study, in which emersed individuals were returned to the sub-merged conditions. Although we detected an increase in overall protein concentration with longeremersion periods, contrary to general expectation, the two dimensional electrophoresis （2DE）-based proteomic analysis did not show significant differences between the treatments at the levelof individual protein spots, even after an emersion period of 24 days. Our results suggest that themetabolism remains unaltered independent of the treatment carried out or the changes are verysubtle and therefore difficult to detect with our experimental design. We conclude that M. neri-toides could be equally adapted to emersion and submersion without drastic physiologicalchanges.
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