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Microbial lipases:Propitious biocatalysts for the food industry

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Food Bioscience 2022年第1期45卷 34-44页

作者： Cleonice aparecida Salgado Clarissa Isabela aparecida dos Santos Maria Cristina Dantas Vanetti Department of Microbiology Universidade Federal de Viçosa(UFV)ViçosaMGBrazil

Lipases(triacylglycerol acylhydrolases,EC 3.***.1.3)are one of the largest groups of enzymes and are used in various industrial *** of microbial origin are currently receiving increased attention for industrial application as microorganisms grow quickly and are easily genetically ***,they offer several advantages,such as catalysis of diverse reactions,high specificity,high yields,low energy consumption and reduced processing time and production *** is a relentless ongoing effort to optimise the production of microbial lipases for potential application in the food *** this context,this review highlights the most promising techniques for producing microbial lipases and the recent applications of these lipases in dairy,oils and fats,bakery and confectionery,meat,flavours and aromas and other food *** lipases are normally obtained by fermentation,but the high costs of carbon and nitrogen sources limit the *** overcome this problem,low-cost agro-industrial residues in the lipase production process are *** obtain lipases with high yields and improved characteristics,the technique of protein engineering is described as promising,and the immobilization method that allows the recycling of lipases to improve their catalytic performance is *** to their catalytic properties and versatility,lipases of microbial origin are considered extremely important catalysts in the food industry,meeting the demand for tastier foods with pleasant aromas and ***,microbial lipases are considered safe and sustainable biocatalysts.

关键词： Microbial lipases Food industry Agro-industrial residue

Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture

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Journal of Environmental Sciences 2015年第10期27卷 102-111页

作者： Jaqueline Bianchi Mario Sérgio Mantovani Maria aparecida Marin-Morales Department of Biology Institute of BiosciencesSao Paulo State University(UNESP) Department of General Biology Biological Science CentreUniv Estadual de Londrina

Based on the concentration of Malathion used in the field, we evaluated the genotoxic potential of low concentrations of this insecticide on meristematic and F1 cells of Allium cepa and on rat hepatoma tissue culture（HTC cells）. In the A. cepa, chromosomal aberrations（CAs）, micronuclei（MN）, and mitotic index（MI） were evaluated by exposing the cells at 1.5,0.75, 0.37, and 0.18 mg/m L of Malathion for 24 and 48 hr of exposure and 48 hr of recovery time. The results showed that all concentrations were genotoxic to A. cepa cells. However,the analysis of the MI has showed non-relevant effects. Chromosomal bridges were the CA more frequently induced, indicating the clastogenic action of Malathion. After the recovery period, the higher concentrations continued to induce genotoxic effects, unlike the observed for the lowest concentrations tested. In HTC cells, the genotoxicity of Malathion was evaluated by the MN test and the comet assay by exposing the cells at 0.09, 0.009, and0.0009 mg/5 m L culture medium, for 24 hr of exposure. In the comet assay, all the concentrations induced genotoxicity in the HTC cells. In the MN test, no significant induction of MN was observed. The genotoxicity induced by the low concentrations of Malathion presented in this work highlights the importance of studying the effects of low concentrations of this pesticide and demonstrates the efficiency of these two test systems for the detection of genetic damage promoted by Malathion.

关键词： HTC Chromosomal aberration Micronuclei Comet assay Pesticide

Mapping of the Brazilian Groups Studying Nanocellulose

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Journal of Renewable Materials 2019年第5期7卷 429-440页

作者： Asaph Armando Jacinto Márcia aparecida da Silva Spinacé Center of Natural and Human Sciences Federal University of ABCSanto AndréSPBrazil

The nanocellulose is a material that has gained much attention in the recent ***,the relevance of Brazil in this field was evaluated concerning the scientific publications in Web of ***,the Brazilian groups were mapped using a bibliometric procedure on these ***,more factors were analyzed from them *** were the sources to extract the nanocellulose in Brazil,the methods to do it,the characterizations to determine its dimensions and the funding agencies of these *** results identified 69 Brazilian ***,the bacterial cellulose was the most common *** the acid hydrolysis was the most used *** its turn,the size characterization was mostly by scanning electron *** last,the most important agencies were the CNPq,the CAPES and the *** these points,it was possible to suggest the opportunities to develop the nanocellulose research in Brazil.

关键词： Nanocellulose bibliometry scientometrics scientific publication web of science

Presence of blaOXA-48-carrying IncF plasmid in an Escherichia fergusonii strain isolated from a sugarcane soil

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Pedosphere 2020年第2期30卷 293-294页

作者： Jessica aparecida Silva MORETTO Joao Pedro Rueda FURLAN Vania Santos BRAZ Tamires aparecida BITENCOURT Eliana Guedes STEHLING Departamento de Analises Clinicas Toxicologicas e BromatologicasFaculdade de Ciencias Farmaceuticas de Ribeirao PretoUniversidade de Sao PauloRibeirao Preto SP 14040-903(Brazil Departamento de Genetica Faculdade de Medicina de Ribeirao PretoUniversidade de Sao PauloRibeirao Preto SP 14040-903(Brazil

Dear Editor,A large number of antimicrobial agents used for the treatment of humans and animals reach water sources and agricultural soils in different ways,including animal ***,antimicrobial-resistant bacteria,carrying important antimicrobial resistance genes(ARGs),circulate between humans,animals,and the environment,giving attention to the concept of One Health(Topp,2017;Tiedje et al.,2019).

关键词： soil soils sugarcane

Recovering Wood Waste to Produce Briquettes Enriched with Commercial Kraft Lignin

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Natural Resources 2021年第5期12卷 181-195页

作者： Carlos Eduardo Silveira da Silva Fernando José Borges Gomes Larisse aparecida Ribas Batalha Roberto Carlos Costa Lelis Ana Márcia Macedo Ladeira Carvalho angélica de Cássia Oliveira Carneiro Alexandre Monteiro de Carvalho Federal Rural University of Rio de Janeiro Forest Products Department Rio de Janeiro Brazil Federal University of Viç osa Forest Engineer Department Vi&#231 osa Minas Gerais Brazil

Aiming to use lignocellulosic biomass as energy source, one of the process that may aggregate values is the densification process, which allows the production of bioenergy using solid fuels, mainly for reducing transportation costs. In this research, solid fuel from co-briquetting of wood residues from sawmill using commercial kraft lignin as binder was investigated. The effects of compression pressure (900, 1200 and 1500 PSI) and briquette formulation (varying wood and kraft lignin proportion) on the quality and characteristics of briquettes were evaluated. The main findings were that briquetting of wood residues with kraft lignin resulted in an improvement of bulk density, strength rupture modulus, low heating value (LHV) and high heating value (HHV). The briquettes using 4% and 6% of kraft lignin, and submitted to 1200 to 1500 PSI, presented higher bulk density and strength resistance, respectively. On the other hand, the heating values showed the highest results with the addition of 2% lignin at 900 PSI, being the legal range for additives in briquettes for many countries such as in European Union.

关键词： Hardwood Biomass Densification Heating Values Solid Fuel

Differential diagnosis between autism spectrum disorder and other developmental disorders with emphasis on the preschool period

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World Journal of Pediatrics 2023年第8期19卷 715-726页

作者： Taynara Amancio de Lima Patricia aparecida Zuanetti Marina Estima Neiva Nunes Ana Paula Andrade Hamad Department of Neurosciences and Behavioral Sciences Ribeirão Preto Medical SchoolUniversity of São PauloAv.Bandeirantes3900Monte AlegreRibeirão PretoSP14049-900Brazil University Hospital Ribeirão Preto Medical SchoolUniversity of São Paulo(HCFMRP-USP)Ribeirão PretoSPBrazil

Background Neurodevelopmental disorders are a heterogeneous group of conditions that manifest as delays or deviations in the acquisition of expected developmental milestones and behavioral *** spectrum disorder(ASD)is a neurodevelopmental disorder characterized by deficits in communication and social interaction and by repetitive and restricted patterns of behavior,interests and *** aim of this review is to discuss the clinical features of the differential diagnoses of ASD that are prevalent among preschoolers,focusing on their similarities and *** sources The international medical literature search was conducted using PubMed and was revised regarding the subject using single and/or combined keywords as follows:differential diagnosis,preschoolers,diagnostic challenge,attention deficit hyperactivity disorder,intellectual disability,high abilities/giftedness,childhood apraxia of speech,social communication disorder,Landau–Kleffner syndrome,stereotyped movement disorder and excessive screen *** We describe conditions commonly found in clinical practice,taking ASD as a *** addressed converging and divergent aspects of behavior,cognition,communication,language,speech,socialization,and stereotypes for the diagnosis of ASD and other disorders identified as potential differential or comorbid *** The ranking and characterization of symptoms appear to be essential for better understanding the underlying common ground between children with developmental disorders and children with ASD,thus properly diagnosing and directing social,professional,or medication *** detailed discussion adds to the literature since,although ASD differential diagnoses are frequently mentioned and discussed in textbooks and journal articles,they rarely occupy a prominent place as we aimed herein.

关键词： Autism spectrum disorder Clinical features Differential diagnosis Neurodevelopmental disorders Preschool

A perivascular epithelioid cell tumor of the stomach:An unsuspected diagnosis

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World Journal of Gastroenterology 2010年第4期16卷 522-525页

作者： Cristina aparecida Troques da Silveira Mitteldorf Dario Birolini Luis Heraldo da Camara-Lopes Laboratory of Surgical and Molecular Pathology Hospital Sírio Libanêsrua Dona Adma Jafet 91So PauloSP 01308-050Brazil Department of Surgery of the University of So Paulo Medical School av. Dr. Enéas de Carvalho Aguiar 25505403-000 So Paulo SP Brazil Hospital Sírio Libanês rua Dona Adma Jafet 91 So Paulo SP 01308-050 Brazil Genoa Biotechnology S.A. Alameda Ministro Rocha Azevedo 346 1st floor 01410-000So Paulo SP 01308-050 Brazil

Perivascular epithelioid cell tumor(PEComa) is a rare mesenchymal neoplasia and currently well recognized as a distinct entity with characteristic morphological,immunohistochemical and molecular *** report a case of PEComa arising in the antrum of a 71-year-old female with *** tumor,located predominantly in the submucosa as a well delimited nodule,measured 3.0 cm in diameter and was completely resected,with no evidence of the disease ***,it was composed predominantly of eosinophilic epithelioid cells arranged in small nests commonly related to variably sized vessels,with abundant extracellular material,moderate nuclear variation and discrete mitotic *** necrosis,angiolymphatic invasion or perineural infil-tration was *** cells were uniformly positive for vimentin,smooth muscle actin,desmin and melan *** unusual,PEComa should be considered in the differential diagnosis of gastric neoplasia with characteristic epithelioid and oncocytic features and prominent vasculature.

关键词： Perivascular epithelioid cell tumor Stomach Gastrointestinal hemorrhage

Search for naive human pluripotent stem cells

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World Journal of Stem Cells 2015年第3期7卷 649-656页

作者： Simone aparecida Siqueira Fonseca Roberta Montero Costas Lygia Veiga Pereira National Laboratory of Embryonic Stem Cell Department of Genetics and Evolutionary Biology University of Sao Paulo Sao Paulo 05508-090 Brazil

Normal mouse pluripotent stem cells were originally derived from the inner cell mass(ICM) of blastocysts and shown to be the in vitro equivalent of those pre-implantation embryonic cells, and thus were called embryonic stem cells(ESCs). More than a decade later, pluripotent cells were isolated from the ICM of human blastocysts. Despite being called human ESCs, these cells differ significantly from mouse ESCs, including different morphology and mechanisms of control of pluripotency, suggesting distinct embryonic origins of ESCs from the two species. Subsequently, mouse pluripotent stem cells were established from the ICMderived epiblast of post-implantation embryos. These mouse epiblast stem cells(Epi SCs) are morphological and epigenetically more similar to human ESCs. This raised the question of whether cells from the human ICM are in a more advanced differentiation stage than their murine counterpart, or whether the available culture conditions were not adequate to maintain those human cells in their in vivo state, leading to a transition into Epi SC-like cells in vitro. More recently, novel culture conditions allowed the conversion of human ESCs into mouse ESC-like cells called nave(or ground state) human ESCs, and the derivation of nave human ESCs from blastocysts. Here we will review the characteristics of each type of pluripotent stem cells, how(and whether) these relate to different stages of embryonic development, and discuss the potential implications of nave human ESCs in research and therapy.

关键词： Naive pluripotent stem cells Epiblast stem cells X chromosome inactivation Human embryonic stem cells

Attributes of stand-age-dependent forest determine technosol fertility of Atlantic forest re-growing on mining tailings in Mariana,Brazil

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Journal of Forestry Research 2022年第1期33卷 103-116页

作者： Pedro Manuel Villa Sebastião Venâncio Martins Aline Pilocelli Gabriel Correa Kruschewsky Andreia aparecida Dias Fabio Haruki Nabeta Forest Restoration Laboratory Department of Forest EngineeringUniversidade Federal de ViçosaViçosaMinas GeraisBrazil Fundação Renova Belo HorizonteMinas GeraisBrazil

Understanding how soil fertility changes due to environmental conditions and stand-age-dependent forest attributes is important for local-scale forest *** evaluated the effects of stand-age-dependent forest attributes(plant community composition and litter stock)on soil and technosol fertility across two second-growth Atlantic forests(SGF)after the deposition of mining tailings in Mariana,southeastern *** hypothesized that technosol fertility in the SGF tailings is positively affected by plant community composition variability,stand age,and litter *** used total exchangeable bases and organic matter as fertility indicators for technosol and soil,and species composition and litter stock as stand-age-dependent forest *** results showed significant differences in the stand-age-dependent forest attributes and soil chemical properties between the two forest patches(SGF tailing and SGF non-tailing)***,there was a marked gradient of litter storage and fertility between soil and technosol that can be important forest recovery indicators for the affected plant ***,according to the tested models,we corroborated the hypothesis that technosol fertility is positively affected by stand age,plant community composition variability,and litter stock,which may contribute considerably to forest recovery on *** results demonstrate that the fertility predictors analyzed to explain the forest recovery on tailings can also be considered as ecological indicators for assessing forest restoration in areas impacted by mining tailings in Mariana.

关键词： Ecological indicators Forest recovery Litter stock Nutrient recovery Technosol