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Exogenous cytokinin application to Actinidia chinensis var. deliciosa ‘Hayward’ fruit promotes fruit expansion through water uptake

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Horticulture Research 2017年第1期4卷 131-141页

作者： Simona Nardozza Helen L Boldingh Mark W Wohlers andrew p gleave Zhiwei Luo Guglielmo Costa Elspeth A MacRae Michael J Clearwater Annette C Richardson The New Zealand Institute for plant&Food Research Limited(pFR) Mt Albert Research CentrePrivate Bag 92169AucklandNew Zealand pFR Ruakura Research CentrePrivate Bag 3123HamiltonNew Zealand Dipartimento di Scienzie Agrarie Universitàdi BolognaVia Fanin 46Bologna40127Italy pFR Te Puke Research CentreTe Puke3182New Zealand pFR Kerikeri Research CentrePrivate Bag 23KerikeriNew Zealand Scion Te Papa Tipu Innovation ParkPrivate Bag 3020RotoruaNew Zealand University of Waikato School of SciencePrivate Bag 3105HamiltonNew Zealand

Exogenous application of a cytokinin-like compound forchlorfenuron(CppU)can promote fruit growth,although often at the expense of dry matter(DM),an important indicator of fruit *** chinensis ***‘Hayward’fruit are very responsive to CppU treatments,but the mechanism underlying the significant fruit weight increase and associated decrease in DM is *** this study,we hypothesised that CppU-enhanced growth increases fruit carbohydrate demand,but limited carbohydrate supply resulted in decreased fruit *** fruit development,CppU effects on physical parameters,metabolites,osmotic pressure and transcriptional changes were assessed under conditions of both standard and a high carbohydrate *** showed that CppU increased fruit fresh weight but the dramatic DM decrease was not carbohydrate *** glucose and fructose concentrations contributed to an increase in soluble carbohydrate osmotic pressure,which was correlated with increased water accumulation in CppU-treated fruit and up-regulation of water channel aquaporin gene pIp2.4 at 49 days after *** analysis suggested that the molecular mechanism contributing to increased glucose and fructose concentrations was altered by carbohydrate *** standard carbohydrate supply,the early glucose increase in CppU fruit was associated with reduced starch synthesis and increased starch *** carbohydrate supply was high,the early glucose increase in CppU fruit was associated with a general decrease in starch synthesis but up-regulation of vacuolar invertase and fructokinase *** conclude that CppU affected fruit expansion by increasing the osmotically-driven water uptake and its effect was not carbohydrate supply-limited.

关键词： CppU Actinidia expansion

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The application of digital holography for accurate three-dimensional localisation of mosquito-bednet interaction

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Light(Advanced Manufacturing) 2022年第3期3卷 13-27页

作者： Matthew L Hall Katherine gleave Angela Hughes philip J McCall Catherine E Towers David p Towers Optical Engineering Group School of EngineeringUniversity of WarwickCoventryCV47ALUK Vector Biology Department Liverpool School of Tropical MedicineLiverpoolL35QAUK

Understanding mosquito interaction with long-lasting insecticidal bednets is crucial in the development of more effective intervention methods to protect humans from malaria *** such,a 240×240×1000 mm laboratory setup for the in-line recording of digital holograms and subsequent in-focus reconstruction and 3D localisation of mosquitoes is *** bednet background removal methods are used to accurately localise a mosquito obscured by a bednet in 3D *** and physical data demonstrate that this method is suitable for mosquitoes positioned 3−1000 mm behind a bednet.A novel post-processing technique,involving a cascade-correlation of a Tamura of Intensity focus metric extracted from digitally reconstructed scenes,accurately localises mosquitoes positioned 35−100 mm behind a bednet from a single digital *** result of this study is a scalable digital holographic methodology to examine mosquito-bednet interaction in 3D at a level of accuracy previously only seen in 2D imaging of mosquitoes in a much smaller volume.

关键词： Digital Holography Metrology Entomology

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