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Method Optimization Study on Isolation of Volatile Compounds by Headspace Solid-Phase Microextraction (HS-SPME) from Custard Apple (<i>Annona squamosa</i>L.) Pulp.

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Journal of Analytical Sciences, Methods and Instrumentation 2020年第3期10卷 59-77页

作者： Mércia de Sousa Galvão Karen Leticia de Santana Juliete Pedreira Nogueira Maria Terezinha Santos leite Neta Narendra Narain departament of Food Engineering Federal University of Paraiba Jo&atilde o Pessoa Brazil Laboratory of Flavor and Chromatographic Analysis PROCTA Federal University of Sergipe S&atilde o Cristóv&atilde o Brazil

Aroma is an important quality parameter, which can help in delaying degradative processes and hence understanding the volatile compounds that are part of an aroma is extremely important. Thus, the aim of this study was to isolate the volatile compounds present in custard apple (Annona squamosa L.) pulp by solid-phase microextraction (SPME) technique using 3 different fibers (DVB/CAR/PDMS, PDMS and PDMS/DVB). An experimental design was set up to evaluate the best extraction conditions wherein the variables were extraction temperature (minimum 28°C, maximum 62°C), ionic strength (minimum 0, maximum 30.0%) and sample concentration (min 1, max 99.0%). The separation of volatiles was achieved in chromatographic columns of different polarity (polar and non-polar) while identification of volatile compounds was performed by high resolution gas chromatography system coupled with mass spectrometry. The results obtained using 3 different fibers revealed the isolation of 71 compounds with fiber DVB/CAR/PDMS, while only 30 and 21 compounds were isolated using fibers PDMS/DVB and PDMS, respectively. The major classes of compounds identified were terpenes, esters, alcohols and aldehydes. Optimum conditions for obtaining higher number of volatiles for non-polar column were 45°C for extraction temperature, 15% of sodium chloride and 50% of pulp amount which resulted in isolation of a maximum of 78 peaks.

关键词： Flavor SPME Response Surface Methodology Volatiles Gas Chromatography-Mass Spectrometry

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Impacts of COVID-19 on children and adolescents:A systematic review analyzing its psychiatric effects

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World Journal of Psychiatry 2022年第11期12卷 1313-1322页

作者： Italo Wanderson de Moura Gabriel Danielly Gonçalves Sombra Lima Jeully Pereira Pires Nélio Barreto Vieira Aloisio Antonio Gomes de Matos Brasil Yara Talita Gomes Pereira Erika Galvao de Oliveira HildsonLeandro de Menezes Nadia Nara Rolim Lima Alberto Olavo Advíncula Reis Ruan Neto Pereira Alves Uanderson Pereira da Silva Jucier Gonçalves Junior Modesto leite Rolim-Neto School of Medicine Faculty of Medicine of Juazeiro do Norte-FMJ/IDOMEDJuazeiro do Norte 63040-360CearaBrazil School of Medicine Federal University of CaririBarbalha 63048-080CearaBrazil Mais Médicos Program Federal Government of Brazil-Ministry of HealthIguatu 63048-080CearaBrazil Julio Alves de Lira Hospital and Maternity Hospital HALHMBelo Jardim 55157-290PernambucoBrazil Nursing department Doctor Leão Sampaio University Center-UNILEAOJuazeiro do Norte 63.041-140CearaBrazil Julio Alves de Lira Hospital and Maternity Hospital JALHMBelo Jardim 55157-290PernambucoBrazil Graduate Program(Post-Doctoral)in Neuro-Psychiatry UFPERecife 50670-901PernambucoBrazil Postgraduate Program(Master’s and Doctorate)in Public Health University of São Paulo-USPSão Paulo 01246-904São PauloBrazil School of Education USPSão Paulo 05508-040São PauloBrazil Internal Medicine-Division of Rheumathology at Hospital das Clínicas São Paulo UniversitySão Paulo 01246-903São PauloBrazil

OBJECTIVE To summarize the most relevant data from a systematic review on the impact of COVID-19 on children and adolescents,particularly analyzing its psychiatric *** This review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses(PRISMA)guidelines and included experimental studies(randomized-individually or pooled-and non-randomized controlled trials),observational studies with a group for internal comparison(cohort studies-prospective and retrospective-and case-control)and qualitative studies in the period from 2021 to *** The search identified 325 articles;we removed 125 *** selected 200 manuscripts,chosen by title and selected *** excluded 50 records after screening titles and abstracts,as they did not meet the inclusion *** retrieved 150 records selected for a full *** excluded 90 text articles and we selected 25 records for the(n)***:Due to the short period of data collection,from 2021 to 2022,there is a possibility of lack of relevant studies related to the mental health care of children and *** addition,there is the possibility of publication bias,such as only significant findings being *** The impact of COVID-19 on the mental health of children and adolescents is of great concern to child and youth *** such as fear,anxiety,panic,depression,sleep and appetite disorders,as well as impairment in social interactions caused by psychic stress,are punctual markers of pain and psychic suffering,which have increasing impacts on the mental health panorama of children and adolescents globally,particularly in vulnerable and socially at-risk populations.

关键词： Child psychiatry Adolescent psychiatry Mental health COVID-19 Kids Teens

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dengue in renal transplant recipients: Clinical course and impact on renal function

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World Journal of Transplantation 2017年第1期7卷 57-63页

作者： Paula Frassinetti Castelo Branco Camur?a Fernandes Reed andréSiqueira Evelyne Santana Gir?o Rainne andréSiqueira Márcia Uchoa Mota Leyla Castelo Branco Fernandes Marques Silvana Cristina Albuquerque andrade Wilson Mendes Barroso S?nia leite Silva Bruno Gomes Rodrigues dos Santos Claúdia Maria Costa de Oliveira Kidney Transplant Unit Hospital Universitário Walter Cantídio Universidade Federal do Ceará department of Nephrology of Universidade Estadual do Ceará Universidade Estadual do Ceará

AIM To present clinical characteristics from renal transplant recipients with dengue fever and its impact on graft *** We retrospectively evaluated 11 renal transplant recipients(RTR) with dengue infection confirmed by laboratory test, between January 2007 and July 2012, transplanted in the Renal Transplant Center of Walter Cantídio University Hospital from Federal University of Ceará. RESULTS Positive dengue serology(IgM) was found in all patients. The mean time between transplant and dengue infection was 43 mo. Fever was presented in all patients. Nine patients presented with classical dengue and two(18%) with dengue hemorrhagic fever. All cases had satisfactory evolution with complete recovery of the symptoms. The time for symptom resolution varied from 2 to 20 d, with an average of 9 d. An increase of creatinine after the infection was observed in three(27.2%) patients with no clinically impact on the kidney graft function. CONCLUSION RTR with dengue infection seems to have a clinical presentation and evolution similar to those seen in the general population, with no long-term damage to patient and to the graft.

关键词： Kidney Renal Transplant dengue Clinical Brazil

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Endoscopic ultrasonography-emerging applications in hepatology

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World Journal of Gastrointestinal Endoscopy 2017年第8期9卷 378-388页

作者： Joana Magalhaes Sara Monteiro Sofia Xavier Sílvia leite Francisca Dias de Castro JoséCotter Gastroenterology department Hospital Senhora da Oliveira School of Medicine University of Minho Life and Health Sciences Research Institute/3B’s PT Government Associate Laboratory

The inspection of the liver is a valuable part of the upper endoscopic ultrasonography(EUS) studies,regardless of the primary indication for the *** detailed images of the liver segments provided by EUS allows the use of this technique in the study of parenchymal liver disease and even in the diagnosis and classification of focal liver *** has also emerged as an important tool in understanding the complex collateral circulation in patients with portal hypertension and their clinical and prognostic ***,EUS-guided portal vein catheterization has been performed for direct portal pressure measurement as an alternative method to evaluate portal *** this review,the authors summarize the available evidence regarding the application of EUS to patients with liver diseases and how we can apply it in our current clinical practice.

关键词： Endoscopic ultrasonography Portal hypertension Gastroesophageal varices Focal liver lesions Liver biopsy

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Orthobiologics in the treatment of hip disorders

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World Journal of Stem Cells 2021年第4期13卷 304-316页

作者： andre Vinicius Saueressig Kruel Lucas leite Ribeiro Paulo David Gusmao Stephany Cares Huber Jose Fabio Santos Duarte Lana department of Orthopedics Regenesis Clínica MédicaBento GonçalvesRS 95700-066Brazil department of Orthopedics Instituto Médico SalusSão PauloSP 01308-050Brazil department of Hematology University of CampinasCampinasSP 13334-170Brazil department of Orthopedics the Bone and Cartilage InstituteIndaiatubaSP 13334-170Brazil

Orthobiologics are biological materials that are intended for the regeneration or healing of bone,cartilage and soft *** this review we discuss the use of orthobiologics for hip disorders providing an *** orthobiologics included in this article are hyaluronic acid,platelet rich plasma,bone marrow,adipose tissue and expanded mesenchymal stem *** explain the concepts and definitions of each orthobiological product,and the literature regarding its use in the hip *** paucity of guidelines for the production and characterization of the biological products leads to uneven results across the *** biologic therapy has indications and benefits;however,noteworthy are the characterization of the orthobiologics,the application method and outcome analysis for further improvement of each technique.

关键词： Orthobiologics Hip disorders Platelet-rich plasma Mesenchymal stem cells Bone marrow Adipose tissue

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High Levels Production of Recombinant Human Activin A—Effect upon <i>in Vivo</i>Follicle Stimulation

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Advances in Bioscience and Biotechnology 2015年第2期6卷 96-104页

作者： Nélia Ferreira de Souza Meire Coelho de Almeida Geralda Walkiria de Araujo Raquel Mariana de Melo leite Ivan Carlos dos Santos Mercia de Paula Lima Jorge Luiz Pesquero Universidade de Mogi das Cruzes Mogi das Cruzes Brazil Faculdade Pitágoras Belo Horizonte Brazil departamento de Fisiologia e Biofísica Instituto de Ciências Biológicas Universidade Federal de Minas Gerais Belo Horizonte Brazil departamento de Engenharia de Biossistemas Universidade Federal de S&atildeo Jo&atildeo del Rei S&atildeo Jo&atildeo del Rei Brazil departamento de Enfermagem Básica Escola de Enfermagem Universidade Federal de Minas Gerais Belo Horizonte Brazil

Activin plays an important role in numerous physiological processes such as cell differentiation and remodeling, regeneration and repair of tissues from various organs, angiogenesis, morphogenesis of glandular organs, pluripotency and differentiation of stem cells, cell adhesion and apoptosis. It participates in reproductive processes like embryogenesis, in the expression of Follicle Stimulating Hormone and Luteinizing Hormone and maturation of ovarian follicles and therefore has application in the area of reproduction of vertebrates. Given the economic importance of activin, we develop an efficient and economical method for the production of recombinant human activin A (rACT), using as expression system the yeast Pichia pastoris. rACT showed biological activity as it induced, on submicromolar dose, the increase of ovarian mass and the ovulation process in a mammal model.

关键词： Recombinant Human Activin Yeast Superovulation

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Expression of Messenger RNA of Oncoproteins E6 and E7 of Human Papilomavirus in Women with Negative Oncotic Cytologies, Epithelial Squamous Atypias of Undefined Significance and Low-Grade Intraepithelial Lesions

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Open Journal of Obstetrics and Gynecology 2015年第1期5卷 36-43页

作者： Daiene Carvalho Casagrande Julisa Chamorro Lascasas Ribalta Katia de Araujo Prado Ferreira leite Manoel Schimidt Neila Maria de Góis Speck department of Gynecology Escola Paulista de Medicina (UNIFESP) Sao Paulo Brazil

Objectives: To verify the prevalence of E6/E7 RNAm expression of HPV in patients with negative cervicovaginal cytology, ASC-US and LSIL;to correlate with negative anatomopathological exams and/or squamous intraepithelial neoplasy grade I (SIN 1) of the lower genital tract (LGT);to relate the RNAm expression with viral infection types;to assess the genotyping in single infections. Methods: Findings from 825 women submitted to E6/E7 RNAm survey and 422 women submitted to LGT biopsies were analyzed. Results: A larger percentage of E6/E7 in ASC-US and LSIL cytologies occurred. Negative results of RNAm expression were in accordance with negative cytologies and negative anatomopathological exam. In positive cases, the infection by a single HPV type was most prevalent, with the type 16 being the most common. Conclusions: the expression of mRNA was most prevalent in ASC-US and LSIL cytologies, comparing with the negative ones. The findings of SIN 1 biopsies were related to the positive expression of mRNA and negative cytologies;the negative expression was in agreement with negative anatomopathological exam. The infection by a HPV type was more frequent in cases of positive expression, the HPV type 16 being most frequently found. Patients with low grade intraepithelial lesion cytologies had a higher percentage of multiple infections.

关键词： E6/E7 RNAm Human Papillomavirus Negative Cytology ASC-US Cytology LSIL Cytology

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A long-term annual water balance analysis of the Aracuai River Basin, Brazil

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Journal of Geographical Sciences 2010年第6期20卷 938-946页

作者： leite Mariangela Garcia Praca FUJACO Maria Augusta Goncalves Federal University of Ouro Preto Geology Department Campos Morro do Cruzeiro 35400-000 Ouro PretoMinas Gerais Brazil

Semi-arid and arid areas exhibit great temporal variability in water availability. In some of these regions, a one- or two-day rainfall is followed by intervening dry periods of variable length. In recent decades, many rivers of the semi-arid portion of the Jequitinhonha Basin have been undergoing a progressive discharge reduction, mainly of their base flow, the Aracuai River is one of them. In order to understand this transformation, a long-term analysis of the annual water balance of the Aracuai River Basin was performed, the results of which are presented herein. Satellite images, hydrometeorological and river discharge data were analyzed; mean and variance tests were conducted to determine temporal homogeneity. Historical pluviometric data analysis shows no corresponding precipitation reduction and temperature undergoes only a slight increase in the same period. On the other hand, evaporation is extremely high, higher than precipitation during most of the year, leaving almost no water for infiltration （aquifer recharge） and runoff. Furthermore, the Aracuai head- waters of its tributaries are now occupied by a monoculture, Eucaliptus sp., used for paper production. Because of the decreased fluvial discharges, its lowlands, usually used for agriculture and pasture, are abandoned and partially eroded.

关键词： river discharge evaporation Jequitinhonha Basin

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Structural Characteristics of Mulato Grass I under Different Cutting Heights

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American Journal of Plant Sciences 2014年第5期5卷 627-635页

作者： Leonardo Assis Dutra Fabianno Cavalcante de Carvalho Eneas Reis leite Magno José Duarte Candido Joao Ambrósio de Araújo Filho Curso de Zootecnia Universidade Estadual Vale do AcaraúSobralBrazil departamento de Zootecnia Universidade Federal do CearáFortalezaBrazil

This work aimed to evaluate the effects of different individual heights of cut on structural characteristics of Brachiaria hibrida cv. Mulato I in four periods of the year. The study was carried out at the Fazenda Experimental Vale do Acaraú, in Sobral, Ceará, Brazil, from January to december, 2008. The experimental design was a split plot, with the plots representing four pasture conditions through manual cuts of 10 cm, 20 cm, 30 cm and 40 cm heights, which were done every 30 days. Each treatment had three replications. The subplots were represented by four periods of evaluation: January-February-March, April-May-June, July-August-September and October-November-december. Fertilization was performed with 350 kg of N/ha, divided into monthly applications. Irrigation was conducted in alternate days during the dry season. The variables studied were: mean height before cutting (cm);dry matter content (%);total dry matter production (t/ha);percentage of leaves;percentage of stems;percentage of dead material;leaf blade/stem ratios;number of total leaves per tiller;number of live leaves per tiller;average size of leaves;and tiller density. Cutting at 10 cm caused a decrease in total dry matter production, underestimating the potential production. Cutting heights between 20 and 30 cm contributed positively to increase the total number of leaves, number of green leaves, average size of the leafs and total dry matter production. Under the present experimental conditions, cutting around 40 cm was not recommended because of a decreasing in the leaf/stem ratio and a increasing in stem fraction and dry matter content, compromising the structure of the canopy.

关键词： Grazing Management Leaf/Stem Ratio Tillers

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Leaf Anatomy of Prosthechea moojenii(Pabst)*** and *** Cath.&***(Orchidaceae)as an Approach to Taxonomy of Species Occurring in Biomes Cerrado and Atlantic Forest of the Bahia

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American Journal of Plant Sciences 2012年第12期3卷 1752-1758页

作者： Filipe de Castro Seixas Silvana Helena Nascimento Monteiro Lazaro Benedito da Silva Kelly Regina Batista leite Instituto de Biologia Departamento de BotanicaLaboratório de Anatomia Vegetal e Identificacao de MadeirasCampus Univer-sitário de OndinaUniversidade Federal da BahiaSalvadorBrasil Instituto de Pesquisa Jardim Botanico do Rio de Janeiro Uni-dade de Botanica SistemáticaRio de JaneiroBrasil

Prosthechea is a neotropical genus, belonging to the family Orchidaceae. The Brazil recorded 32 species of the genus of which nine occur in the state of Bahia, including P. silvana and P. moojenii. The first, P. silvana is epiphytic, endemic Atlantic Forest areas of South and Southeast of the Bahia and morphologically similar to P. moojenii, rupicolous species considered endemic to the Chapada Diamantina. There are doubts whether these two species are really distinct, because they differ only as to the way of life and aspects related to the size of the inflorescence and floral structure. Thus the anatomical study of these two species, emerges as a tool that aims to investigate the delimitation of these taxa. For this study, the sheet was cut freehand with a razor blade to obtain cross sections and treated by chemical means to obtain paradermic sections, all the material was stained with safranine/Astra blue (safrablau) and safranin, respectively, and the sections were then mounted between a semipermanent and cover slipped slides. The results allowed to distinguish P. silvana of the P. moojenii through the following characteristics observed in the leaf (thickening of the cell walls of epidermal cells, amount and disposition of sclerenchyma fibers and fiber arrangement of vascular and vascular bundles in the mesophyll) and root (number of arcs of xylem and phloem, thickening of cells median cortex), infers taxonomic characters for the genus. The data indicated anatomical distinction between the two species studied here of the Prosthechea, however, suggest the anatomical analysis of other organs to reproductive and vegetative.

关键词： Laeliinae Flora of Bahia Velamen Endemism

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