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Coat protein of Chinese wheat mosaic virus upregulates and interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenase,a negative regulator of plant autophagy,to promote virus infection

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Journal of Integrative Plant Biology 2022年第8期64卷 1631-1645页

作者： Erbo Niu Chaozheng Ye Wanying Zhao Hideki Kondo Yunfeng Wu Jianping Chen Ida Bagus andika Liying Sun State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F UniversityXi'an 712100China Institute of Plant Science and Resources Okayama UniversityKurashiki 710‐0046Japan State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro‐products Institute of Plant VirologyNingbo UniversityNingbo 315211China College of Plant Health and Medicine Qingdao Agricultural UniversityQingdao 266109China

Autophagy is an intracellular degradation mechanism involved in antiviral defense,but the strategies employed by plant viruses to counteract autophagy-related defense remain unknown for the majority of the ***,we describe how the Chinese wheat mosaic virus(CWMV,genus Furovirus)interferes with autophagy and enhances its infection in Nicotiana *** two-hybrid screening and in vivo/in vitro assays revealed that the 19 k Da coat protein(CP19 K)of CWMV interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenases(GAPCs),negative regulators of autophagy,which bind autophagy-related protein 3(ATG3),a key factor in ***19 K also directly interacts with ATG3,possibly leading to the formation of a CP19 K–GAPC–ATG3 ***19 K–GAPC interaction appeared to intensify CP19 K–ATG3 ***,CP19 K expression upregulated GAPC gene transcripts and reduced autophagic ***,the silencing of GAPC genes in transgenic *** reduced CWMV accumulation,whereas CP19 K overexpression enhanced ***,our results suggest that CWMV CP19 K interferes with autophagy through the promotion and utilization of the GAPC role as a negative regulator of autophagy.

关键词： antiviral defense autophagy plant virus protein interaction viral coat protein

Enhancing of nominal characteristic trajectory following control for motion systems

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Journal of Systems Engineering and Electronics 2017年第6期28卷 1221-1235页

作者： Fitri Yakub Shamsul Sarip andika Aji Wijaya Yasuchika Mori Malaysia-Japan International Institute of Technology University of Technology MalaysiaKuala Lumpur 54100Malaysia Razak School of Engineering and Advanced Technology University of Technology MalaysiaKuala Lumpur54100Malaysia Department of Industrial Engineering University of Business and TechnologyJeddah 21361Saudi Arabia Graduate School of System Design Tokyo Metropolitan UniversityTokyo 191-0065Japan

The goal of this paper is to enhance a practical nominal characteristic trajectory following(NCTF) controller that is specifically designed for two-mass point-to-point positioning systems. A nominal characteristics trajectory contained in the NCTF controller acts as movement/motion reference and a compensator is utilized to force the object to detect and follow the reference/desired trajectory. The object must follow and track closely and should be as fast as possible. The NCTF controller is designed with two different intelligent based compensator approaches which are fuzzy logic and extended minimal resource allocation network. The proposed controller which is NCTF are compared with the conventional proportional integral compensator. Then the results of simulation are discussed for the positioning performances. The inertia variations due to the effect of the design parameters are also assessed to see the robustness of controllers. The results show that the NCTF control method designed from an intelligent based compensator has a better positioning performance in terms of percentage of overshoot, settling time, and steady state error than the classical based compensator.

关键词： two-mass rotary system intelligent approach classi cal method mechanical vibration nominal characteristic trajectory following

Coat protein of rice stripe virus enhances autophagy activity through interaction with cytosolic glyceraldehyde-3-phosphate dehydrogenases,a negative regulator of plant autophagy

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Stress Biology 2023年第1期3卷 35-51页

作者： Wanying Zhao Li Wang Lipeng Li Tong Zhou Fei Yan Heng Zhang Ying Zhu Ida Bagus andika Liying Sun State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYangling 712100ShaanxiChina Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjing 210095China Institute of Plant Virology Ningbo UniversityNingbo 312362China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Institute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhou 310021China College of Plant Health and Medicine Qingdao Agricultural UniversityQingdao 266109China

Viral infection commonly induces autophagy,leading to antiviral responses or conversely,promoting viral infection or *** this study,using the experimental plant Nicotiana benthamiana,we demonstrated that the rice stripe virus(RSV)coat protein(CP)enhanced autophagic activity through interaction with cytosolic glyceraldehyde-3-phosphate dehydrogenase 2(GAPC2),a negative regulator of plant autophagy that binds to an autophagy key factor,autophagy-related protein 3(ATG3).Competitive pull-down and co-immunoprecipitation(Co-IP)assays showed that RSV CP activated autophagy by disrupting the interaction between GAPC2 and *** RSV CP mutant that was unable to bind GAPC2 failed to disrupt the interaction between GAPC2 and ATG3 and therefore lost its ability to induce *** CP enhanced the autophagic degradation of a viral movement protein(MP)encoded by a heterologous virus,citrus leaf blotch virus(CLBV).However,the autophagic degradation of RSV-encoded MP and RNA-silencing suppressor(NS3)proteins was inhibited in the presence of CP,suggesting that RSV CP can protect MP and NS3 against autophagic ***,in the presence of MP,RSV CP could induce the autophagic degradation of a remorin protein(NbREM1),which negatively regulates RSV infection through the inhibition of viral cell-to-cell ***,our results suggest that RSV CP induces a selective autophagy to suppress the antiviral factors while protecting RSV-encoded viral proteins against autophagic degradation through an as-yet-unknown *** study showed that RSV CP plays dual roles in the autophagy-related interaction between plants and viruses.

关键词： RSV Coat protein Autophagy GAPC ATG3

Environmental and Management Factors That Influence Commelina Species in Selected Agro-Ecological Zones in Western Kenya

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American Journal of Plant Sciences 2022年第6期13卷 884-911页

作者： Runyambo Irakiza andika Darius Watako Arnold Mwonga Samuel Musili Paul Kirika Paul Department of Plant Animal and Food Science Jaramogi Oginga Odinga University of Science and Technology (JOOUST) Bondo Kenya Department of Crops Horticulture and Soils Egerton University Njoro Kenya East African Herbarium National Museums of Kenya Nairobi Kenya

Commelina species are plant resources full of promise as future food and feed that thrive in diverse ecosystems. They are medicinal plants, leafy vegetables, forage for ruminants, feed for cricket insects, crop protection, and fuel. However, information regarding factors driving Commelina in agro-ecological zones in Western Kenya is lacking. Therefore, we investigated the diversity of Commelina species, the composition of associated weed species as well as environmental and management factors affecting their diversity and distribution based on 22 variables from 12 production sites. In the survey, 115 species belonging to 30 families were recorded of which 11 Commelina species were identified. Among Commelina species, Commelina diffusa and Commelina benghalensis var. benghalensis (non-hybrid variant) had higher relative density. Multiple linear regressions revealed that the environment (exchangeable sodium percentage, magnesium, soil pH, and total nitrogen) and management (agriculture system type) variables exert a stronger effect on the diversity of Commelina species. Detrended Correspondence Analysis detected different ecological conditions for Commelina species and the composition of associated weed species. The forward selection based on Canonical Correspondence Analysis indicated that the distribution of Commelina species responded significantly to soil pH, available phosphorous, total nitrogen, fertility, and crop spacing. Partitioning variation showed the great importance to the environment than management (10.57% versus 5.97%). The low shared variance (environment × management) was -0.4%, indicating that the two factors have a more individualistic than interactive nature. However, the 83.86% that remained unexplained was attributed to stochastic variation or unmeasured variables. This study suggests that the identified five important variables affecting the distribution of Commelina species will certainly contribute to the prioritization of ecological aspects

关键词： Commelina Canonical Correspondence Analysis (CCA) Environment Management Weed Vegetation

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花生“一拌三喷”绿色防控技术示范效果

中国植保导刊 2022年第2期42卷 84-88页

作者：陈芳龙曲诚怀王秀连迟玉成 Ida Bagus andika 曹欣然青岛农业大学植物医学学院山东青岛266109 山东省烟台市牟平区农业技术推广中心山东烟台264001 山东省烟台市牟平区水道镇政府山东烟台264109 山东省花生研究所山东青岛200120

为探索花生不同生育阶段的最佳植保解决方案,于2019—2020年进行了“一拌三喷”绿色防控技术示范。结果表明,29%噻虫·咯·霜灵悬浮种衣剂拌种后,能够有效提高花生出苗率,示范区出苗率75.0%,较常规区和对照区增幅分别为17.1%和27.9%,差... 详细信息

为探索花生不同生育阶段的最佳植保解决方案,于2019—2020年进行了“一拌三喷”绿色防控技术示范。结果表明,29%噻虫·咯·霜灵悬浮种衣剂拌种后,能够有效提高花生出苗率,示范区出苗率75.0%,较常规区和对照区增幅分别为17.1%和27.9%,差异显著;同时还可减少根部病害的发生,未发生茎基腐病。示范区出苗齐,主根粗壮,侧根数量多(162根/株),示范区与常规区和对照区差异显著。200 g/L氟酰羟·苯甲唑悬浮剂对花生叶斑病防效显著(94.7%),示范区花生落叶少,叶斑病病情指数仅为2.6,防效显著优于常规区和对照区。花生产量显著提高,示范区两年平均单产达到332.8 kg/667m^(2),较对照区和常规区分别增加107.7 kg和31.6 kg,增产幅度达到47.8%和10.5%。采用“一拌三喷”绿色防控技术,示范区平均比对照区净增收入534元/667m^(2),比常规区净增收入206元/667m^(2),经济效益显著。建议在花生种植区域大面积推广应用以29%噻虫·咯·霜灵悬浮种衣剂拌种、200 g/L氟酰羟·苯甲唑悬浮剂叶面喷雾为主的“一拌三喷”花生绿色防控技术。

关键词：花生绿色防控花生病害产量

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一款六足机器人的运动学分析与运动控制研究

一款六足机器人的运动学分析与运动控制研究

作者：安迪（JULPRI andika）北京理工大学

学位级别：硕士

机械臂(或机械腿、机械爪)在机器人的应用中较为常见,是大部分机器人执行抓取、行走或攀爬任务的重要执行机构。尤其对于腿式机器人而言,许多研究文献已经阐明了如何将三自由度机械臂应用于多足机器人(尤其是六足机器人)的行走与避障任... 详细信息

机械臂(或机械腿、机械爪)在机器人的应用中较为常见,是大部分机器人执行抓取、行走或攀爬任务的重要执行机构。尤其对于腿式机器人而言,许多研究文献已经阐明了如何将三自由度机械臂应用于多足机器人(尤其是六足机器人)的行走与避障任务中。在某种程度上而言,六足机器人的运动方向与行走步态可以通过分析六足机器人三自由度机械臂的正逆运动学规律来确定。本文以一款典型六足机器人的三自由度单腿执行机构作为研究对象,根据该六足机器人单腿的结构尺寸参数与自由度配置方式,以Denavit-Hartenberg参数理论为基础进行单腿正逆运动学规律的研究与分析;在此过程中,构建了机器人单腿结构末端执行器的位姿与关节角的正逆运动学方程,借助Matlab与RoboAnalyzer对所得正逆运动学方程进行了仿真验证;在仿真验证的基础上,以Arduino Mega 2560作为六足机器人的控制核心,根据六足机器人的工作使命、功能需求与控制核心的片上资源,设计了基于该核心的机器人主控电路,将逆运动学规律实现代码化并写入控制核心,且引入三角步态,通过搭载的超声波传感器,实现了机器人在多种地形下的稳定行走与避障功能。本文进行的正逆运动学理论探索与仿真验证,其结果证明了六足机器人单腿结构设计的合理性与运动学分析的正确性。实物样机的实验结果有效直观的验证了在基于Arduino Mega 2560控制核心的六足机器人上采用三角步态行走的可行性,并通过了实物避障实验,验证了基于超声波传感器避障算法的有效性。本文工作具有一定的创新性与实用性,可为相关研究提供参考与帮助。

关键词：六足机器人运动学分析三角步态避障 Arduino

小麦黄花叶病毒（WYMV）RNA2基因功能的研究

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小麦黄花叶病毒（WYMV）RNA2基因功能的研究

中国植物病理学会2011年学术年会

作者：孙丽英向荣 Da Bagus andika 陈剑平浙江省农业科学院病毒与生物技术研究所

小麦黄花叶病毒（Wheat yellow mosaic virus,WYMV）是引起我国冬小麦菌传病毒病的主要病原。WYMV属于马铃薯Y病毒科大麦黄花叶病毒属。其基因组由两条单链正义RNA组成,RNA1编码的蛋白有:P3、7K、CI、14 K、NIa-VPg、NIb-VPg和CP,RNA2编...

小麦黄花叶病毒（Wheat yellow mosaic virus,WYMV）是引起我国冬小麦菌传病毒病的主要病原。WYMV属于马铃薯Y病毒科大麦黄花叶病毒属。其基因组由两条单链正义RNA组成,RNA1编码的蛋白有:P3、7K、CI、14 K、NIa-VPg、NIb-VPg和CP,RNA2编码P1和P2蛋白,其中P1蛋白与已知马铃薯Y病毒属成员编码的HC-Pro具有相似的结构域。目前,HC-Pro是研究最深入的病毒蛋白之一,它在病毒的复制、运输、移动、RNA沉默抑制和病毒致病性等方面有着重要的功能。实验室早期研究发现,同属的大麦黄花叶病毒（Barley yellow mosaic virus,BYMV）连续人工摩擦接种后病毒RNA2编码的P2蛋白碳端发生缺失,含有缺失P2碳端的BYMV不影响其侵染循环,并且致病力有所增强。为进一步分析WYMV致病机制,利用昆虫细胞表达系、嵌合体病毒载体PVX及双分子荧光互补（BiFC）技术,我们对WYMV RNA2编码的两个蛋白质进行了功能分析及验证。发现P1蛋白与P2蛋白氮端共表达时可加重PVX在本氏烟上引起的症状。但P1,P2都没有抑制农杆菌共表达GFP介导的基因沉默现象。利用双分子荧光互补（BiFC）技术在植物和昆虫细胞表达体系分析病毒编码蛋白质之间及与寄主因子之间的相互作用,结果发现WYMV P1除进行自我互作外,还与WYMV VPg发生特异性互作,通过亚细胞定位分析WYMV P1、VPg分别单独表达和一起表达时在细胞中的分布,发现P1可以改变VPg在亚细胞中的分布。根据上述研究结果,推测WYMV P1可能通过调节VPg亚细胞分布,通过协助VPg而实现其功能。并讨论了此过程中某些寄主因子的参与。

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菌传小麦病毒病的低温致病机制研究

菌传小麦病毒病的低温致病机制研究

中国小麦花叶病毒外壳蛋白的翻译策略与小麦品种抗病性相关

中国植物病理学会2012年学术年会

作者：孙丽英 Ida Bagus andika 陈剑平浙江省农业科学院病毒学与生物技术研究所

小麦黄花叶病毒（Wheat yellow mosaic virus,WYMV）和中国小麦花叶病毒（Chinesewheat mosaic virus,CWMV）是引起我国菌传小麦病毒病的主要病原,主要分布在黄淮,长江中下游等冬小麦种植区。此区是我国小麦单产最高的种植区,其气候特... 详细信息

小麦黄花叶病毒（Wheat yellow mosaic virus,WYMV）和中国小麦花叶病毒（Chinesewheat mosaic virus,CWMV）是引起我国菌传小麦病毒病的主要病原,主要分布在黄淮,长江中下游等冬小麦种植区。此区是我国小麦单产最高的种植区,其气候特点温度适宜,雨水充足有时有涝灾发生。而菌传病毒病暴发流行的主要气候条件是秋冬雨水充分,春季小麦返青拔节期的持续低温。当春季温度回暖,连续1周温度超过摄氏23℃时,病毒复制受到抑制,出现"隐症现象"。"隐症现象"具有很强的迷惑性,隐症之后,人们往往忽略了病害对小麦生产的影响。由于土传小麦病毒病的发病严重影响小麦根系发展,干扰植株的营养运输,同时影响小麦正常分蘖,因此,病害对小麦后期的抗逆反应,产量、品质都有不同程度的影响。针对土传小麦病毒病低温致病的的特点展开研究,不仅能够分析小麦土传病毒病的致病机理,而且能够通过此现象分析小麦抗病及病后恢复的分子机制。本研究首先明确了基因沉默抗病途径对病毒低温复制及病症表达的影响,其次分析了病毒编码的病程蛋白在不同温度时对症状表达的影响,最后分析了病毒蛋白在不同温度下的表达情况,发现CWMV外壳蛋白在温度上升至23℃时蛋白质的稳定性发生明显变化,并对其降解途径进行了深入分析。

关键词：小麦菌传病毒低温致病性基因沉默病毒蛋白稳定性

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中国小麦花叶病毒外壳蛋白的翻译策略与小麦品种抗病性相关