Coat protein of Chinese wheat mosaic virus upregulates and interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenase,a negative regulator of plant autophagy,to promote virus infection

作     者：Erbo Niu Chaozheng Ye Wanying Zhao Hideki Kondo Yunfeng Wu Jianping Chen Ida Bagus Andika Liying Sun Erbo Niu;Chaozheng Ye;Wanying Zhao;Hideki Kondo;Yunfeng Wu;Jianping Chen;Ida Bagus Andika;Liying Sun

作者机构：State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant ProtectionNorthwest A&F UniversityXi'an 712100China Institute of Plant Science and ResourcesOkayama UniversityKurashiki 710‐0046Japan State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro‐productsInstitute of Plant VirologyNingbo UniversityNingbo 315211China College of Plant Health and MedicineQingdao Agricultural UniversityQingdao 266109China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第8期

页      面：1631-1645页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 

基　　金：supported in part by the National Natural Science Foundation of China(32170163) the Basic Scientific Research Foundation,Ministry of education of China(Z109021801)to Liying Sun The program of introducing Talents of Innovative Discipline to Universities(project 111)(B18042)and(2016KW-069)to Liying Sun 

主　　题：antiviral defense autophagy plant virus protein interaction viral coat protein 

摘      要：Autophagy is an intracellular degradation mechanism involved in antiviral defense,but the strategies employed by plant viruses to counteract autophagy-related defense remain unknown for the majority of the ***,we describe how the Chinese wheat mosaic virus(CWMV,genus Furovirus)interferes with autophagy and enhances its infection in Nicotiana *** two-hybrid screening and in vivo/in vitro assays revealed that the 19 k Da coat protein(CP19 K)of CWMV interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenases(GAPCs),negative regulators of autophagy,which bind autophagy-related protein 3(ATG3),a key factor in ***19 K also directly interacts with ATG3,possibly leading to the formation of a CP19 K–GAPC–ATG3 ***19 K–GAPC interaction appeared to intensify CP19 K–ATG3 ***,CP19 K expression upregulated GAPC gene transcripts and reduced autophagic ***,the silencing of GAPC genes in transgenic *** reduced CWMV accumulation,whereas CP19 K overexpression enhanced ***,our results suggest that CWMV CP19 K interferes with autophagy through the promotion and utilization of the GAPC role as a negative regulator of autophagy.