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云光技术 1996年第1期28卷 46-50页

作者： yurk.,OK 李继东

本文介绍了无色差面复消色差物镜玻璃的选择方法；给出了采用Ｖ．Ｎ．Ｃｈｕｒｉｌｏｖｓｋｉｉ提出的计算方法根据校正位置色差、二级光谱色差和球面像差的校正条件，便可确定物镜所有结构参数的计算公式；列举了一个物镜的计算实例。

关键词：色差物镜复消色差

Neuroprotective role of protein tyrosine phosphatase-1B in rod photoreceptor neurons

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Protein & Cell 2013年第12期4卷 890-892页

作者： Raju v.S.Rajala Ammaji Rajala Department of Ophthalmology University of Oklahoma Health Sciences CenterOklahoma CityOklahoma 73104USA Department of Physiology University of Oklahoma Health Sciences CenterOklahoma CityOklahoma 73104USA Department of Cell Biology University of Oklahoma Health Sciences CenterOklahoma CityOklahoma 73104USA Dean A.McGee eye Institute Oklahoma CityOklahoma 73104USA

Dear Editor,Protein tyrosine phosphatase-1B(PT-P1B)is an abundant,widely expressed non-receptor tyrosine phosphatase,which is thought to be a key negative regulator of insulin signaling(Tonks,2003).Increased and prolo... 详细信息

关键词： PTP1B insulin of

ISOLATION PURIFICATION AND PRELIMINARY CHARACTERIZATION OF A PROTEIN SPECIFIC FOR WHEAT PLANTS·NONINFECTED ey PSEUDOCERCOSPORELLA HERPOTRICHOIDES

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植物病理学报 1993年第1期 37-42页

作者： v.Lind G.Wenzel D-8059 Grunbach Germany Institute for Resistance Genetics

A wide range of plants respond to pathogen attack with production ofpathogenesis-related proteins This paper describes another kind of proteins-healthy specific proteins,which only appear in healthy wheat plants,whenplants are infected by ***,they disappear One of this kind ofproteins,Pc2,was purified by DEAE-cellulose ion exchange,Sephadex G-100

关键词： ISOLATION PURIFICATION AND PRELIMINARY CHARACTERIZATION OF A PROTEIN SPECIFIC FOR WHEAT PLANTS NONINFECTED ey PSEUDOCERCOSPORELLA HERPOTRICHOIDES

Conservation and divergence of Grb7 family of Ras-binding domains

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Protein & Cell 2012年第1期3卷 60-70页

作者： Raju v.S.Rajala Ammaji Rajala vivek K.Gupta Department of Ophthalmology University of Oklahoma Health Sciences CenterOklahoma CityOK 73104USA Department of Cell Biology University of Oklahoma Health Sciences CenterOklahoma CityOK 73104USA Dean A.McGee eye Institute Oklahoma CityOK 73104USA

Ras proteins are signal-transducing GTPases that cycle between inactive GDP-bound and active GTP-bound *** is a prolific signaling molecule interacting with a spectrum of effector molecules and acting through more than one signaling *** Ras-effector proteins contain a Ras-associating(RA)domain through which these associate with Ras in a GTP-dependent *** RA domain is highly conserved among the members of the growth factor receptor-bound(Grb)7 family of proteins which includes Grb7,Grb10 and *** laboratory has reported an unusual observation that RA domain of Grb14 binds to the C-terminal nucleotide binding site of cyclic nucleotide gated channel(CTRCNGA1)and inhibits the channel *** modeling of the CTR-CNGA1 displays 50%---70%tertiary structural similarity towards Ras *** named this region as Ras-like domain(RLD).The interaction between RA-Grb14 and RLD-CNGA1 is mediated through a simple protein-protein interaction temporally and spatially regulated by light and *** is interesting to note that Grb14 binds to GTPase-mutant Rab5,a Ras-related small GTPase whereas Grb10 binds only to GTP-bound form of active Rab5 but not to GTPase-defective mutant *** results suggest that Grb14 might have been evolved later in the evolution that binds to both Ras and nucleotide binding proteins such as *** studies also suggest that eukaryotic CNG channels could be evolved through a gene fusion between prokaryotic ion channels and cyclic nucleotide binding proteins,both of which might have undergone several sequence variations for functional adaptation during evolution.

关键词： growth factor receptor-bound protein 14 Ras-associating domain cyclic nucleotide gated channel rod outer segments tyrosine kinase signaling Ras proteins

Contrasting plant growth performance of invasive polyploid and native diploid Prosopis is mediated by the soil bacterial community

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Ecological Processes 2023年第1期12卷 193-203页

作者： Rishabh Kaushik Meesha Sharma Ch.v.Ramana Ch.Sasikala Maharaj K.Pandit Jealott’s Hill International Research Centre WarfieldBracknell RG426EYEnglandUK Department of Environmental Studies University of DelhiDelhi 110007India Department of Plant Sciences School of Life SciencesUniversity of HyderabadP.O.Central UniversityHyderabad 500046India Bacterial Discovery Laboratory Centre for EnvironmentInstitute of Science and TechnologyJ.N.T.University HyderabadKukatpallyHyderabad 500085India Jindal School of Environment&Sustainability OP Jindal Global UniversitySonipatHaryana 131001India

Background Soil microbial communities affect above-ground plant diversity and community composition by influencing plant growth *** studies have tested the effect of soil bacterial microbiome on growth performance of native and invasive plants,but the influence of specific bacterial isolates has not been ***,we investigated the effects of soil bacterial exclusion by soil sterilization and by inoculation of Streptomyces rhizobacterial isolates on the growth performance of native and invasive Prosopis *** Plant growth performance of invasive *** was significantly reduced when grown in sterilized soils,whereas native *** showed enhanced growth performance in the sterilized *** grown in the soil inoculated with the specific Streptomyces isolate from ***(PJ1),the growth performance of invasive *** was significantly enhanced while that of native *** seedlings was significantly ***,inoculation of *** and *** seedlings with Streptomyces isolate from the rhizosphere of native ***(PC1)had no significant effect on the growth performances either of *** or *** Our study reveals that invasive *** experiences positive feedback from the non-native soil bacterial community,while the native *** experiences negative feedback from its soil bacterial *** results provide fresh experimental evidence for the enemy release hypothesis,and further our understanding of the contrasting growth-promoting effects of differentially recruited microbial species belonging to the same genus(Streptomyces)in the rhizospheres of alien invasive and native plants.

关键词： Soil bacteria Streptomyces Invasive Prosopis juliflora Prosopis cineraria Soil microbiota

How genetic errors in GPCRs affect their function:Possible therapeutic strategies

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Genes & Diseases 2015年第2期2卷 108-132页

作者： Henriette Stoy vsevolod v.Gurevich University of Tu¨bingen Tu¨bingen 72074Germany Department of Pharmacology Vanderbilt UniversityNashvilleTN 37232USA

Activating and inactivating mutations in numerous human G protein-coupled receptors(GPCRs)are associated with a wide range of disease *** we use several class A GPCRs with a particularly large set of identified disease-associated mutations,many of which were biochemically characterized,along with known GPCR structures and current models of GPCR activation,to understand the molecular mechanisms yielding pathological *** on this mechanistic understanding we also propose different therapeutic approaches,both conventional,using small molecule ligands,and novel,involving gene therapy.

关键词： Activation Agonist Mutation Gene therapy Genetic disorder GPCR

Mechanism involved in the modulation of photoreceptor-specific cyclic nucleotidegated channel by the tyrosine kinase adapter protein Grb14

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Protein & Cell 2011年第11期2卷 906-917页

作者： vivek K.Gupta Ammaji Rajala Karla K.Rodgers Raju v.S.Rajala Department of Ophthalmology University of Oklahoma Health Sciences CenterOklahoma CityOK 73104USA Department of Cell Biology University of Oklahoma Health Sciences CenterOklahoma CityOK 73104USA Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences CenterOklahoma CityOK 73104USA Dean A.McGee eye Institute University of Oklahoma Health Sciences CenterOklahoma CityOK 73104USA

We recently found that growth factor receptor-bound(Grb)protein 14 is a novel physiological modulator of photoreceptor specific cyclic nucleotide-gated channel alpha subunit(CNGA1).Grb14 promotes the CNG channel closure through its Ras-associating(RA)*** the current study we show that this RA domain-mediated inhibition of rod CNG channel is electrostatic in ***14 competes with cGMP for the CNGA1 binding pocket and electrostatically interacts with Arg^(559) through a negatively chargedβ-turn at its RA ***,the three Glu residues(180–182)in Grb14 are absolutely critical for electrostatic interaction with the cGMP binding pocket and resultant *** study also demonstrates that substitution of Lys^(140) for Ala or in combination with polyglutamte mutants of Grb14 results in a significantly reduced binding with *** results suggest that in addition to Glu^(180–182) and Lys^(140),other residues in Grb14 may be involved in the electrostatic interaction with *** RA domain is highly conserved among the members of Grb7 family of proteins,which includes Grb7,Grb10 and ***,only Grb14 is able to modulate the channel activity,but not Grb7 and *** together,it suggests the existence of a divergence in RA domains among the members of the Grb7 family.

关键词： growth factor receptor-bound protein 14(Grb14) Ras-associating domain cyclic nucleotide gated channel rod outer segments tyrosine kinase signaling

Laser Machined Fiber‑Based Microprobe: Application in Microscale Electroporation

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Advanced Fiber Materials 2022年第4期4卷 859-872页

作者： Jongwoon Kim Yajun Zhao Shuo Yang Ziang Feng Anbo Wang Rafael v.Davalos Xiaoting Jia Bradley Department of Electrical and Computer Engineering Virginia TechBlacksburgVA 24060USA Department of Biomedical Engineering and Mechanics Virginia TechBlacksburgVA 24061USA

Microscale electroporation devices are mostly restricted to in vitro experiments(i.e.,microchannel and microcapillary).Novel fiber-based microprobes enable in vivo microscale electroporation and arbitrarily select the cell groups of interest to *** developed a flexible,fiber-based microscale electroporation device through a thermal drawing process and femtosecond laser micromachining *** fiber consists of four copper electrodes(80μm),one microfluidic channel(30μm),and has an overall diameter of 400μ*** dimensions of the exposed electrodes and channel were customizable through a delicate femtosecond laser *** feasibility of the fiber probe was validated through numerical simulations and in vitro *** reversible and irreversible microscale electroporation was observed in a 3D collagen scaffold(seeded with U251 human glioma cells)using fluorescent *** ablation regions were estimated by performing the covariance error ellipse method and compared with the numerical *** computational and experimental results of the working fiber-based microprobe suggest the feasibility of in vivo microscale electroporation in space-sensitive areas,such as the deep brain.

关键词： Fiber probe Polymer Micromachine Irreversible electroporation Reversible electroporation Microscale electroporation

Arrestin-mediated signaling: Is there a controversy?

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World Journal of Biological Chemistry 2018年第3期9卷 25-35页

作者： vsevolod v Gurevich Eugenia v Gurevich Department of Pharmacology Vanderbilt University

The activation of the mitogen-activated protein(MAP) kinases extracellular signal-regulated kinase(ERK)1/2 was traditionally used as a readout of signaling of G protein-coupled receptors(GPCRs) via arrestins, as opposed to conventional GPCR signaling via G proteins. Several recent studies using HEK293 cells where all G proteins were genetically ablated or inactivated, or both non-visual arrestins were knocked out, demonstrated that ERK1/2 phosphorylation requires G protein activity, but does not necessarily require the presence of non-visual arrestins. This appears to contradict the prevailing paradigm. Here we discuss these results along with the recent data on gene edited cells and arrestinmediated signaling. We suggest that there is no real controversy. G proteins might be involved in the activation of the upstream-most MAP3Ks, although in vivo most MAP3K activation is independent of heterotrimeric G proteins, being initiated by receptor tyrosine kinases and/or integrins. As far as MAP kinases are concerned, the best-established role of arrestins is scaffolding of the three-tiered cascades(MAP3K-MAP2 K-MAPK). Thus, it seems likely that arrestins, GPCRbound and free, facilitate the propagation of signals in these cascades, whereas signal initiation via MAP3K activation may be independent of arrestins. Different MAP3Ks are activated by various inputs, some of which are mediated by G proteins, particularly in cell culture, where we artificially prevent signaling by receptor tyrosine kinases and integrins, thereby favoring GPCR-induced signaling. Thus, there is no reason to change the paradigm: Arrestins and G proteins play distinct non-overlapping roles in cell signaling.

关键词： G protein-coupled receptors Arrestin G protein Signaling Extracellular signal-regulated kinase 1/2 c-Jun N-terminal kinase 3

Improvement of tissue analysis and classification using optical coherence tomography combined with Raman spectroscopy

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Journal of Innovative Optical Health Sciences 2015年第4期8卷 10-19页

作者： Chih-Hao Liu Ji Qi Jing Lu Shang Wang Chen Wu Wei-Chuan Shih Kirill v.Larin Department of Biomedical Engineering University of Houston 3605 Cullen Blvd. HoustonTexas 77204-5060USA Department of Electrical and Computer Engineering University of Houston 4800 Calhoun Rd. HoustonTexas 77204USA Department of Molecular Physiology and Biophysics Baylor College of Medicine One Baylor Plaza HoustonTexas 77030USA

Optical coherence tomography(OCT)provides significant advantages of high resolution(approaching the histopathology level)realtime imaging of tsess without use of contrast *** on these advantages,the microstructural features of tumors can be visualized and detected ***,it is still not clinically accepted for tumor margin delin-eation due to poor specificity and *** contrast,Raman spectroscopy(RS)can obtain tissue information at the molecular level,but does not provide real-time inaging ***,combining OCT and RS could provide *** this end,we present a tissue analysis and dassification method using both the slope of OCT intensity signal vs depth and the principle components from the RS spectrum as the indicators for tissuse *** goal of this study was to understand prediction accuracy of OCT and combined OCT/RS method for dassification of optically similar tisues and *** pilot experiments were performed on mouse kidneys,livers,and small intestines(SIs).The prediction accuracy with five-fold cross validation of the method has been evaluated by the support vector machine(SvM)*** results demonstrate that tissue characterization based on the OCT/RS method was superior compared to using OCT structural information *** combined OCT/RS method is potentially useful as a noninvasive optical biopsy technique for rapid and automatic tissue characterization during surgery.

关键词： OCT signal slope principle component analysis multi-support vector machine Raman spectra