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利用先进磨削技术生产高精度铰刀

现代制造 2020年第8期 30-30页

作者： anca Group 不详

铰刀在当今的机械加工车间中起着不可或缺的作用,对于机械师的工具库至关重要,因此行业内广泛流传着这样一句话:如果想要一个精确的孔,请从一把完美的铰刀开始。铰孔,本质上是扩孔的过程。铰刀设计用于将钻孔尺寸进行少量的放大,但具有... 详细信息

铰刀在当今的机械加工车间中起着不可或缺的作用,对于机械师的工具库至关重要,因此行业内广泛流传着这样一句话:如果想要一个精确的孔,请从一把完美的铰刀开始。铰孔,本质上是扩孔的过程。铰刀设计用于将钻孔尺寸进行少量的放大,但具有高精度,能够确保孔的侧面具有光滑的表面光洁度。正确地使用铰刀,可将数千个孔扩孔至高精度孔,而不会降低性能。铰刀是目前现有的最精确的孔加工刀具,如果在金属加工中使用铰刀,通常根据ISO标准将其用于H6/H7的高精度公差。ISO和ANSI公制标准中它们均指可以自由组装和拆卸的紧固件。

关键词：表面光洁度磨削技术机械加工车间高精度孔铰孔 ISO标准金属加工铰刀设计

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升级智能工厂实现数控机床的自动化生产

现代制造 2020年第5期 40-40页

作者： anca Group anca Group

随着技术的发展,在未来的工厂里,自动化设备将会取代大部分人工劳动力。拥有一个由磨床组成的集成网络,会让工厂在制造工艺、生成知识产权、刀具设计、客户响应度、循环周期和盈利能力等诸多方面更加高效。自动化磨床将在生产之余处理... 详细信息

随着技术的发展,在未来的工厂里,自动化设备将会取代大部分人工劳动力。拥有一个由磨床组成的集成网络,会让工厂在制造工艺、生成知识产权、刀具设计、客户响应度、循环周期和盈利能力等诸多方面更加高效。自动化磨床将在生产之余处理完很多繁重复杂的任务。

关键词：刀具设计数控机床自动化生产自动化设备循环周期集成网络智能工厂知识产权

High levels of homocysteine downregulate apolipoprotein E expression via nuclear factor kappa B

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World Journal of Biological Chemistry 2016年第1期7卷 178-187页

作者： Violeta G Trusca Adina D Mihai Elena V Fuior Ioana M Fenyo anca V Gafencu Department of Genomics Transcriptomics and Molecular TherapiesInstitute of Cellular Biology and Pathology"Nicolae Simionescu" of the Romanian Academy School of Biological and Biomedical Sciences Durham University

AIM: To investigate the effect of high homocysteine(Hcy) levels on apolipoprotein E(apoE) expression and the signaling pathways involved in this gene ***: Reverse transcriptase polymerase chain reaction(RT-PCR) and Western blot were used to assess apo E expression in cells treated with various concentrations(50-500 μmol/L) of Hcy. Calcium phosphatetransient transfections were performed in HEK-293 and RAW 264.7 cells to evaluate the effect of Hcy on apoE regulatory elements [promoter and distal multienhancer 2(ME2)]. To this aim, plasmids containing the proximal apoE promoter [(-500/+73)apoE construct] alone or in the presence of ME2 [ME2/(-500/+73)apoE construct] to drive the expression of the reporter luciferase gene were used. Co-transfection experiments were carried out to investigate the downstream effectors of Hcymediated regulation of apoE promoter by using specific inhibitors or a dominant negative form of IKβ. In other co-transfections, the luciferase reporter was under the control of synthetic promoters containing multiple specific binding sites for nuclear factor kappa B(NF-κB), activator protein-1(AP-1) or nuclear factor of activated T cells(NFAT). Chromatin immunoprecipitation(ChI P)assay was accomplished to detect the binding of NF-κB p65 subunit to the apoE promoter in HEK-293 treated with 500 μmol/L Hcy. As control, cells were incubated with similar concentration of cysteine. NF-κB p65 proteins bound to DNA were immunoprecipitated with anti-p65 antibodies and DNA was identified by PCR using primers amplifying the region-100/+4 of the apoE gene. RESULTS: RT-PCR revealed that high levels of Hcy(250-750 μmol/L) induced a 2-3 fold decrease in apoE m RNA levels in HEK-293 cells, while apo E gene expression was not significantly affected by treatment with lower concentrations of Hcy(100 μmol/L). Immunoblotting data provided additional evidence for the negative role of Hcy in apoE expression. Hcy decreased apoE promoter activity, in the presen

关键词： Apolipoprotein E Homocysteine Nuclear factor kappa B Gene regulation MAPK/ERK kinase

黑曲霉MIUG 1.15生产胞外转化酶的研究

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生物技术 1999年第2期9卷 14-16页

作者：钦传光丹.娃楞亭娜巴日姆.加布里拉尼古拉乌.安卡湖北工学院生物工程系加拉兹大学食品工业系

研究黑曲霉ＭＩＵＧ１．１５产生转化酶的条件和特性。表面培养时转化酶的产率较高。每隔６ｄ更换一次培养基，菌种连续发酵４５—５０ｄ后，转化酶活力比最初的高４．７倍。此后，ｐＨ值陡然下降，转化酶迅速失活。转化酶最适宜温度４... 详细信息

研究黑曲霉ＭＩＵＧ１．１５产生转化酶的条件和特性。表面培养时转化酶的产率较高。每隔６ｄ更换一次培养基，菌种连续发酵４５—５０ｄ后，转化酶活力比最初的高４．７倍。此后，ｐＨ值陡然下降，转化酶迅速失活。转化酶最适宜温度４５℃，ｐＨ值４．５，７０℃下热稳定（６０ｍｉｎ内的失活率为５４％）。

关键词：黑曲霉转化酶表面培养发酵条件培养基