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转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化（英文）

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Engineering 2024年第1期 57-68页

作者： Vedrana Vi?i? Bo?kor Nika Foglar Goran Josipovi? Marija Klasi? ana Vuji? Branimir Plav?a Toma Keser Samira Smajlovi? Aleksandar Vojta Vlatka Zoldo? Laboratory for Epigenetics Department of Biology Faculty of Science University of Zagreb Genos Glycoscience Research Laboratory Faculty of Pharmacy and Biochemistry University of Zagreb

Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2) are key transcription factors that regulate a complex gene network in the liver,creating a regulatory transcriptional *** Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase *** in silico analysis of HNF1A,HNF4A,and FOXA2 binding to the ten candidate glyco-genes studied in this work confirms a significant enrichment of these transcription factors specifically in the *** previous studies identified HNF1A as a master regulator of fucosylation,glycan branching,and galactosylation of plasma ***,we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan *** used the state-of-the-art clustered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9) molecular tool for the downregulation of the HNF1A,HNF4A,and FOXA2 genes in HepG2 cells-a human liver cancer cell *** results show that the downregulation of all three genes individually and in pairs affects the transcriptional activity of many gly co-genes,although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan *** effect is better seen as an overall change in the total HepG2 N-glycome,primarily due to the extension of biantenna ry *** propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan *** also propose a model showing feedback loops with the mutual activation of HNF1A-FOXA2 and HNF4A-FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.

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基于遗传算法的电动汽车能量数学建模及优化方法

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自动化与仪器仪表 2024年第1期 79-84页

作者：黄阿娜习璐咸阳职业技术学院陕西咸阳712000

针对基于规则的能量管理策略中不易优化的问题,提出一种基于遗传算法的电动汽车能量优化模型。首先确定遗传算法的种群规模,然后通过优化参数选择及编码,初始化种群,适应度函数确定,确定选择、交叉和变异算子,遗传算法的改进和设定迭代... 详细信息

针对基于规则的能量管理策略中不易优化的问题,提出一种基于遗传算法的电动汽车能量优化模型。首先确定遗传算法的种群规模,然后通过优化参数选择及编码,初始化种群,适应度函数确定,确定选择、交叉和变异算子,遗传算法的改进和设定迭代次数构建电动汽车能量管理优化模型,最后在WLTC循环工况、CLTC循环工况和NEDC循环工况下进行电动汽车的能量消耗仿真实验。仿真结果表明,本研究提出的优化模型相对于基于平均分配的电动汽车能量优化模型,WLTC循环工况下电动汽车的驱动电机总耗能降低了5.62%,CLTC循环工况下总耗能降低了6.83%、 NEDC循环工况下耗能降低了4.01%。本研究的优化策略能够减少电动汽车能量优化管理中计算量,提高优化精度,适应性良好,节能效果好,应用前景广阔。

关键词：遗传算法电动汽车能量管理节能

Concentric Centrifugal Figurate Erythema as an Unusual COVID-19 Evanescent Skin Manifestation in a Patient With CD3^(+)/CD45^(+)Lymphocyte Infiltration:A Case Report

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International Journal of Dermatology and Venereology 2024年第2期7卷 115-118页

作者： Lucía Achell Achell-Nava José Luis Zaldivar-Fujigaki Rebeca Pérez-Cabeza de Vaca ana Maria Espinosa García Luis Montiel López Dermatology Department National Medical Center 20 de NoviembreISSSTEMexico City 03229Mexico Biomedical Research Department National Medical Center 20 de NoviembreISSSTEMexico City 03100Mexico Clinical Pharmacology Division General Hospital of MexicoMexico City 06720Mexico Internal Medicine Department National Medical Center 20 de NoviembreISSSTEMexico City 03100Mexico

Introduction:Coronavirus disease 2019(COVID-19)may be associated with various skin *** them,figurate erythema(FE)is rare;only a few clinical reports have been ***,we reported an unusual concentric FE lesions and their immunohistochemical profile in a patient with *** presentation:A 52-year-old woman was diagnosed with *** month later,she developed asymptomatic annular erythematous concentric ring lesions on her inner right *** margin of every ring increased in coloration,and the lesions then completely resolved in 2 weeks.A biopsy revealed vasculitis of small vessels,an increase in the fluorescence intensity of CD3 and CD45,and negativity for COVID-19 ***:The results showed an unusual FE that has a clinical mixed type between erythema annulare centrifugum and erythema gyratum repens;the physiopathological mechanism in the FE due to COVID-19 is unknown;one theory involves rapid viral passage into the cutaneous vascular system that triggers an immune and inflammatory *** the results exhibited a negative COVID-19 RNA in the tissue,the CD3^(+)/CD45^(+)lymphocyte infiltration could explain the clinical pattern as a direct inflammatory response in the small vessel or as a delayed immune ***:Our findings suggest an immunohistochemical small vessel vasculitis pattern with an unusual clinical FE that differs from other types of COVID-19-associated FE or erythema reported previously.

关键词： erythema COVID-19 leukocytoclastic vasculitis skin manifestation CD3 complex CD45 antigen

Perspective on automated in vivo drug screening using the chodl mutant zebrafish line

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Neural Regeneration Research 2022年第11期17卷 2437-2438页

作者： ana-Maria Oprisoreanu Center for Regenerative Therapies Dresden(CRTD) TU Dresden

Since the early 2000s, the popularity of zebrafish in in vivo drug screening has shown a substantial *** zebrafish has become an important screening tool covering a wide range of tissue-specific pathologies and diseas... 详细信息

关键词： fish vivo screening

发展清洁能源对减污降碳的影响机理与效应分析

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河北环境工程学院学报 2024年第3期34卷 17-22页

作者：殷阿娜曹瑞娜河北地质大学经济学院河北石家庄050031

基于中国30个省份2011—2022年的面板数据,构建双向固定效应模型、调节效应模型,实证分析发展清洁能源对减污降碳的影响。结果表明:发展清洁能源对减污降碳具有显著促进作用;发展清洁能源对减污降碳的影响存在地理区位异质性、经济发展... 详细信息

基于中国30个省份2011—2022年的面板数据,构建双向固定效应模型、调节效应模型,实证分析发展清洁能源对减污降碳的影响。结果表明:发展清洁能源对减污降碳具有显著促进作用;发展清洁能源对减污降碳的影响存在地理区位异质性、经济发展阶段异质性和资源依赖属性异质性;环境规制对清洁能源发展促进减污降碳具有显著正向调节作用。

关键词：减污降碳环境规制双向固定效应模型清洁能源

围术期神经肌肉阻滞的管理:欧洲麻醉和重症监护学会临床指南

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中华麻醉学杂志 2024年第6期44卷 641-656页

作者： Thomas Fuchs-Buder Carolina S.Romero Heidrun Lewald Massimo Lamperti Arash Afshari ana-Marjia Hristovska Denis Schmartz Jochen Hinkelbein Dan Longrois Maria Popp Hans Dde Boer Massimiliano Sorbello Radmilo Jankovic Peter Kranke 法国南锡大学医学院附属医院麻醉学、重症监护与围术期医学科法国南锡54000 巴伦西亚大学综合医院麻醉与重症监护科西班牙巴伦西亚46014 慕尼黑工业大学麻醉学与重症监护科德国慕尼黑80333 克利夫兰阿布扎比诊所麻醉学研究所阿联酋阿布扎比31250 哥本哈根大学医院小儿和产科麻醉科丹麦哥本哈根2100 哥本哈根大学医院麻醉学与重症监护科丹麦赫维德2650 布鲁塞尔自由大学Erasme医院麻醉科比利时布鲁塞尔1070 科隆大学医院麻醉学与重症监护科德国科隆50937 巴黎大学附属比查特克劳德伯纳德医院麻醉学与重症监护医学科法国巴黎75018 维尔茨堡大学医院麻醉学、危重病医学、急诊医学和疼痛医学科德国维尔茨堡97080 格罗宁根大学医院麻醉学、疼痛医学与程序性镇静与镇痛科荷兰格罗宁根9713 GZ 卡坦尼亚大学医院麻醉与重症监护科意大利卡坦尼亚95123 尼什大学医学院尼什临床中心麻醉学与重症治疗科塞尔维亚尼什18000

近来的研究表明,由于围术期神经肌肉阻滞管理不当,肌肉松弛药(肌松药)残留阻滞及其术后并发症的发生率居高不下。需要重视神经肌肉功能监测仪、肌松药及其拮抗剂的合理应用,以利于改善神经肌肉阻滞管理。欧洲麻醉和重症监护学会(Europea... 详细信息

近来的研究表明,由于围术期神经肌肉阻滞管理不当,肌肉松弛药(肌松药)残留阻滞及其术后并发症的发生率居高不下。需要重视神经肌肉功能监测仪、肌松药及其拮抗剂的合理应用,以利于改善神经肌肉阻滞管理。欧洲麻醉和重症监护学会(European Society of anaesthesiology and Intensive Care,ESAIC)首次发布围术期神经肌肉阻滞管理临床指南,旨在为临床医生提供最佳医疗管理的循证建议,以提高围术期患者安全。本指南主要聚焦在3个临床问题:肌松药是否有助于成人气管插管?神经肌肉阻滞深度是否影响腹部手术患者的预后?肌松药残留阻滞的诊断和治疗策略有哪些?根据PICO原则(患者、干预、对照、结局;patient,intervention,comparator,outcome;PICO)构建临床问题并进行文献检索。初始纳入的临床研究为24 000篇,采用逐步筛选策略,最终临床研究88篇纳入指南分析。应用GRADE方法学(Grading of Recommendations,Assessment,Development and Evaluation,GRADE)对结局指标证据、质量等级进行评价。运用两轮德尔菲法收集专家组建议并形成统一推荐:R1推荐应用肌松药辅助气管插管(1A)。R2推荐应用肌松药以减少气管插管导致的咽部和(或)喉部损伤(1C)。R3推荐在快速顺序诱导插管(rapid sequence induction intubation,RSII)时使用短效肌松药,例如琥珀胆碱1 mg/kg或罗库溴铵0.9~1.2 mg/kg(1B)。R4推荐在需要改善外科手术条件时,加深神经肌肉阻滞深度(1B)。R5尚无足够的证据推荐深度神经肌肉阻滞可减轻术后疼痛或降低围术期并发症的发生率(2C)。R6推荐在拇内收肌处刺激尺神经,定量监测神经肌肉阻滞深度以排除肌松药残留阻滞(1B)。R7推荐应用舒更葡糖钠逆转甾体类肌松药(罗库溴铵、维库溴铵)诱导的深、中及浅度神经肌肉阻滞(1A)。R8推荐在使用新斯的明前,TOFr自行恢复至>0.2,并继续定量监测神经肌肉功能,直至TOFr超过0.9(1C)。

关键词：神经肌肉阻滞神经肌肉功能肌松药临床指南罗库溴铵琥珀胆碱外科手术定量监测

Oil recovery tests with ionic liquids: A review and evaluation of 1-decyl-3-methylimidazolium triflate

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Petroleum Science 2022年第4期19卷 1877-1887页

作者： Alba Somoza Alberto Arce ana Soto CRETUS Department of Chemical EngineeringUniversidade de Santiago de CompostelaE-15782Santiago de CompostelaSpain School of Engineering Universidade de A CorunaE-15403FerrolSpain

The main advantages of the use of ionic liquids in enhanced oil recovery are their tunability and stability in harsh environmental conditions. In this work, a comprehensive review of ionic liquids proposed to improve current chemical oil recovery methods has been presented, focusing on core flooding experiments. With an almost infinite number of possible ionic liquids, the amount of experiments carried out up to now has been very limited. However, results are promising, with additional recovery after secondary flooding of up to 32% of the original oil in place. Most formulations with ionic liquids have been proposed for sandstone reservoirs, the number of studies with carbonate cores being very scarce. The possibilities of a new room temperature surface active ionic liquid, 1-decyl-3-methylimidazolium triflate,for this application were analyzed. It was shown that it is able to drastically reduce the water/oil interfacial tension. An optimized formulation was proposed for carbonate reservoirs. After secondary flooding with brine, an additional recovery of 10.5% of original oil in place was achieved at room conditions. A combination of the proposed method followed by a polymer flooding step with polyacrylamide led to a lesser but still significant recovery, reducing the costs associated to the ionic liquid.

关键词： Enhanced oil recovery(EOR) Ionic liquids Core flooding Interfacial tension 1-Decyl-3-methylimidazolium triflate

analysis of the bacterial community composition in acidic well water used for drinking in Guinea-Bissau,West Africa

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Journal of Environmental Sciences 2014年第8期26卷 1605-1614页

作者： ana Machado Adriano A.Bordalo Laboratory of Hydrobiology and Ecology Institute of Biomedical SciencesUniversity of PortoPorto 4050-313Portugal CIIMAR/CIMAR-Interdisciplinary Centre of Marine and Environmental Research University of PortoPorto 4050-123Portugal

Potable water is a resource out of reach for millions worldwide,and the available water may be chemically and microbiologically compromised. This is particularly acute in Africa,where water-networks may be non-existent or restricted to a small fraction of the urban population,as in the case of Guinea-Bissau,West Africa. This study was carried out seasonally in Bolama（11°N）,where unprotected hand-dug wells with acidic water are the sole source of water for the population. We inspected the free-living bacterial community dynamics by automated rRNA intergenic spacer analyses,quantitative polymerase chain reaction and cloning *** results revealed a clear seasonal shift in bacterial assemblage composition and microbial abundance within the same sampling site. Temperature,pH and turbidity,together with the infiltration and percolation of surface water,which takes place in the wet season,seemed to be the driving factors in the shaping and selection of the bacterial community and deterioration of water quality. analysis of 16 S rDNA sequences revealed several potential pathogenic bacteria and uncultured bacteria associated with water and sediments,corroborating the importance of a culture-independent approach in drinking water monitoring.

关键词： Water quality Microbial community ARISA qPCR Drinking water Africa

Academic Stress Assessment Using Virtual Reality as an Educational Tool in Spine Surgery

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Open Journal of Modern Neurosurgery 2024年第2期14卷 114-123页

作者： Diana Chávez Lizárraga Jesús Alberto Pérez Contreras Fernando Alvarado Gómez Evelyn Quintero Medina Emmanuel Cantú Chávez Iván Ulises Sámano López ana Sofía Peña Blesa Department of Neurosurgery ángeles Pedregal Ciudad de México México Department of Traumatology and Orthopedics Hospital Santa Fe Bogotá Colombia

Introduction: The evaluation of academic stress in medical students and residents is a topic of significant interest, given the considerable challenges they face during their learning process with traditional teaching methods. The use of technologies like virtual reality presents an opportunity to enhance their skills through simulations and training. The main objective of this study is to qualitatively assess the stress levels experienced by medical students and residents by integrating virtual reality into their current learning methods, aiming to improve their ability to manage stressors in their practice. Material and Methods: A questionnaire was conducted with 12 medical students and 12 Traumatology and orthopedics residents. The purpose of the questionnaire was to evaluate the levels of academic stress using the SISCO inventory. The stress levels were calculated by transforming average values into percentages, and the following criteria were assigned: 0 to 33% for Mild Stress, 34 to 66% for Moderate Stress, and 67 to 100% for Deep Stress. Then, a virtual reality class focused on spine surgery was provided. Both medical students and residents were trained using the Non Nocere SharpSurgeon software platform and Oculus Quest 2 virtual reality glasses. At the end of the session, a second questionnaire related to the practice with virtual reality was conducted with the same evaluation criteria and a comparative analysis was carried out. Results: 12 undergraduate students from Hospital Angeles Mexico, CDMX and 12 traumatology and orthopedics residents at Hospital Santa Fe, Bogota were evaluated. The students in CDMX reported an average qualitative stress of 28.50% during habitual practices, which decreased to an average of 14.67% after virtual reality practice. Residents in Bogotá experienced an average qualitative stress of 30.50% with their current learning methods but this reduced to an average of 13.92% after using virtual reality. These findings indicate that t

关键词： Virtual Reality Academic Stress Learning Strategies Spine Surgery Training