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Journal of Cancer Therapy 2014年第4期5卷 307-314页

作者： ana paula Montaldi Julia Alejandra Pezuk Elza Tiemi Sakamoto-Hojo Luiz Gonzaga Tone María Sol Brassesco Department of Biology Faculty of Philosophy Sciences and Letters at Ribeirao Preto University of Sao Paulo Sao Paulo Brazil Department of Genetics Faculty of Medicine of Ribeirao Preto University of Sao Paulo Sao Paulo Brazil Department of Pediatrics Faculty of Medicine of Ribeirao Preto University of Sao Paulo Sao Paulo Brazil

The outcome of patients with osteosarcoma has not significantly improved in the last three decades. Therefore, there is still a need for the development of more effective therapeutic strategies. Methoxyamine (MX) is a base excision repair (BER) inhibitor that has shown anticancer potential by sensitizing a variety of tumor cells to ionizing radiation and chemotherapeutic drugs. In the present study, the in vitro antiproliferative effects of MX were evaluated in two osteosarcoma cell lines, HOS and MG-63. Evaluation of the influence on radiosensitivity and drug interactions in simultaneous treatments with methotrexate, doxorubicin, and cisplatin was also performed. Exposure to MX significantly decreased cell proliferation and mediated a substantial increase of apoptosis. Moreover, our results showed that MX synergized with ionizing radiation in both cell lines while potentiated the antitumor effects of cisplatin and methotrexate. Altogether, the results presented herein demonstrate the feasibility of inhibiting the BER pathway, which may in future be a promising strategy for overcoming intrinsic tumor resistance and to improve the outcome of patients with osteosarcoma.

关键词： Osteosarcoma Methoxyamine BER Cell Lines

Linking the spatiotemporal variation of litterfall to standing vegetation biomass in Brazilian savannas

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Journal of Plant Ecology 2020年第5期13卷 517-524页

作者： Alan N.Costa Jeane R.Souza Karyne M.Alves Anderson Penna-Oliveira Geisciele paula-Silva Ingrid S.Becker Kelly Marinho-Vieira ana L.Bonfim Alessandra Bartimachi Ernane H.M.Vieira-Neto Instituto de Biologia Universidade Federal de UberlândiaUberlândia 38405-320MGBrazil Instituto de Biotecnologia Universidade Federal de GoiásCatalão 75704-000GOBrazil Department of Wildlife Ecology and Conservation University of FloridaGainesvilleFL 32611USA

Aims Litterfall at a global scale is affected by climate,edaphic features and vegetation structure,with litter production increasing from grasslands to forests following the rise in standing ***,at landscape scales,the same relationship between litter production and vegetation structure has rarely been studied and comparisons of litterfall patterns between adjacent,structurally distinct communities are ***,we use a standardized methodology to describe the structural differences among four savanna physiognomies and analyze their relationship with changes in litterfall across the *** We evaluated the woody vegetation structure and composition in 48 sites,equally distributed across four physiognomies and monitored the monthly litter production from April 2014 to March *** Findings Results showed that the density,basal area,cylindrical volume and aboveground biomass of woody vegetation differ among physiognomies,increasing consistently from cerrado ralo,cerrado típico,cerrado denso and cerradã***,we found a strong and positive relationship between aboveground biomass and annual litter production,with litter yield increasing from 0.9 to 8.4 Mg ha^(−1)across different physiognomies,following the increment in vegetation *** production was seasonal and similar among vegetation types,increasing during the dry *** comprised the dominant fraction(approx.85%)and litterfall seasonality primarily resulted from the concentration of leaf shedding during dry ***,the temporal pattern of litterfall throughout the year showed a gradual reduction in the seasonality from open to closed vegetation types,likely following the decrease of deciduous species abundance in the plant *** results showed that changes in vegetation structure may affect spatial and temporal litterfall patterns in different physiognomies,which co-occur across the Cerrado landscape,with potential implications for the overall f

关键词： Cerrado ecosystem functioning habitat heterogeneity Neotropical savannas nutrient cycling

Feasibility Assessment of Sugarcane Expansion in Southwest Goiás, Brazil Based on the GIS Technology

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Journal of Geographic Information System 2016年第2期8卷 149-162页

作者： Bruno Cardoso Pedrosa Tuani Cristine Lima de Souza ana paula Dias Turetta Heitor Luis da Costa Coutinho Department of Environmental Engineering of Pontifícia Universidade Católica of Rio de Janeiro Rio de Janeiro Brazil Department of Geography of Rio de Janeiro Federal University Rio de Janeiro Brazil Brazilian Agricultural Research Corporation Nacional Center for Soil Research (EMBRAPA Soils) Rio de Janeiro Brazil

The current study approaches the sugarcane culture expansion in Southwestern Goiás, especially in Mineiros, Quirinópolis and Rio Verde counties, which represent different times and responses to this process. The current logistics structure and future prospects for sugarcane and its derivatives transportation are studied at national level with emphasis to the aforementioned micro-region. Maps showing land use and land cover in three different years were generated from Landsat TM-5 satellite images and they were used to analyze the dynamics of changes in land use and in land cover. The region is marked by strong and rapid growth in the agricultural sector and its sugar-energy industry has been expanding in recent years, although with different aspects among its counties. Since it is a promising region in this sector, due to the favorable soil and weather conditions to the crop, the region requires more investment and planning in logistics to ensure production flow and to make it stronger within domestic and foreign markets.

关键词： GIS Land Use and Land Cover Sugarcane Expansion Sustainability

Profile of Patients Treated in Outpatient Vestibular Rehabilitation at a Hospital in São Paulo

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International Journal of Clinical Medicine 2019年第1期10卷 9-15页

作者： Bárbara Vieira Coró Miguel Angelo Hyppolito Camila Giacomo Carneiro Barros ana paula do Rego André Faculty of Medicine of Ribeirã o Preto University of S&atilde o Paulo Ribeir&atilde o Preto Brazil Department of Ophthalmology Otorhinolaryngology and Head and Neck Surgery Faculty of Medicine of Ribeir&atilde o Preto University of S&atilde o Paulo Ribeir&atilde o Preto Brazil Hospital of Clinical of Ribeirã o Preto Faculty of Medicine of Ribeir&atilde o Preto University of S&atilde o Paulo Ribeir&atilde o Preto Brazil

Introduction: Currently it is very common that the search for diagnosis and treatment for curing diseases can cause vertigo or dizziness. Objective: This study sought to characterize the profile of patients seen in the clinic for vestibular rehabilitation in a tertiary hospital in the last 10 years. Methods: Survey questionnaires of patients with dizziness. Results: The prevalence of treated subjects was female 65.3% (N = 439). The average age was 54.9 years old. Dizziness type roundabout was more prevalent 33.04% (N = 222). Tinnitus occurred in 58.33% of the population. The time of most observed dizziness was less than 5 years 70.68% (N = 475). Neurovegetative symptoms appeared in 63.98% (N = 430) of the population. The bilateral normal hearing was more standard 35.26% (N = 237). The topographic diagnosis of peripheral origin was the most usual 65.47% (N = 440). Conclusion: The peripheral otoneurologic involvement was more prevalent in this population, affecting more women at an average age of 54.9 years old. The most prevalent symptoms were vertigo, nausea, tachycardia, sweating, vomiting and tinnitus, characterizing the peripheral vestibular impairment. The time of dizziness was less than five years. Audiological standard normal curve was predominant and the symptom of tinnitus was the most reported. The most prevalent comorbidity was Hypertension.

关键词： Dizziness Epidemiology Rehabilitation

Electrospun PLLA Membranes for Caffeine Delivery: Diffusional Approach

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Journal of Biomedical Science and Engineering 2017年第12期10卷 563-574页

作者： ana paula S. Immich José Antonio Tornero Francesc Cano Casas Manuel J. Lis Arias Institut d’Investigació Tèxtil i Cooperació Industrial de Terrassa Universitat Politècnica de Catalunya Terrassa Spain Chemical Engineering Department Universitat Politècnica de Catalunya Terrassa Spain

One of the great advantages of electrospun fibers is the large tridimensional area produced, capable of storing any type of material. The aim of our investigation is to study the electrospinning technique to produce polymer membranes for drug delivery applications, given their large surface area and ability to transport a bioactive compound. A mathematical modeling of the delivery system kinetics was also studied to find the mechanism that controls the releasing process. Results showed that electrospinning could provide regular and smooth membranes suitable for drug delivery processes. The mathematical modeling also proved that thicker PLLA membranes exhibited a Fickian diffusion behavior during the drug transport, presenting better control in drug delivery processes.

关键词： Diffusion Polymer Nano Escale Kinetics

Optimization of the Cultivation of Donor Mesenchymal Stromal Cells for Clinical Use in Cellular Therapy

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CellBio 2014年第1期3卷 25-33页

作者： Vanessa Valim Bruna Amorin Annelise Pezzi Maria Aparecida Lima da Silva ana paula Alegretti Lucia Silla Cellular Therapy Center Hospital de Clinicas de Porto Alegre Porto Alegre Brazil Post-Graduation in Medicine: Medical Sciences Federal University of Rio Grande do Sul Porto Alegre Brazil Cellular Therapy Center Hospital de Clinicas de Porto Alegre Porto Alegre Brazil Post-Graduation in Medicine: Medical Sciences Federal University of Rio Grande do Sul Porto Alegre Brazil Hematology and Bone Marrow Transplantation Hospital de Clinicas de Porto Alegre Porto Alegre Brazil

Mesenchymal stromal cells(MSC)have shown their benefits in graft-versus-host disease(GVHD), with five unsettled matters: 1) MSCs expansion in medium with Fetal Bovine Serum(FBS)and its risk of xenoreaction;2) The number of cells indicated for therapy that is relatively high, with the need to optimize the expansion, number and time wise;3) The utilization of third party donors;4) Culture passage number(P);and 5) Source of the cells. This study was designed to determine the superiority of the Platelet Lysates(PL)over FBS on the expansion of MSC, the optimal cell’ plating density and days between each pass, and to investigate if donor total nucleated cells(TNC)obtained from the washouts of discharged bags and filters of hematopoietic stem cell transplantation(HSCT)can be expanded to be used at clinical grade. TNC were removed, plated and after the first passage were cultivated in different concentrations with FBS or PL, and the number of days to reach 80% of confluence was observed. Next, cultures with the same plating density were fed either with PL or with FBS and after seven days counted to analyze how much they had grown in that period. The proliferation of mesenchymal stromal cells in the presence of PL and SFB was averaged 11.88 and 2.5 times, respectively, in a period of 7 days. The highest concentration of plating cells using PL took less time to reach confluence as compared with the three lower ones. This study suggests that the PL is the best choice as a supplement to expand MSC and to allow the proliferation of enough number of MSC at P2 for clinical use.

关键词： Platelet Lysate MSC Optimization Cell Therapy

Pre-formulation Study for Preparation of Mucoadhesive Buccal Tablets Containing Nystatin and Cashew Gum by Direct Compression

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Journal of Physical Science and Application 2019年第1期9卷 1-14页

作者： ana paula de SáPinto Abrahao Magalhaes Flávia Almada do Carmo Claudia Regina Elias Mansur Institute of Macromolecules Federal University of Rio de JaneiroRio de Janeiro 21941-598Brazil Faculty of Pharmacy Federal University of Rio de JaneiroRio de Janeiro 21941-599Brazil Program of Materials Engineering and Metallurgy Technology CenterAlberto Luiz Institute of CoimbraPost-Graduation and Engineering Research-COPPEFederal University of Rio de JaneiroRio de Janeiro 21941-594Brazil

Cashew gum is a branched chain heteropolysaccharide extracted from the cashew tree (anacardium occidentale L.).Purified cashew gum (PCG) is free of plant contaminants and is highly *** studies have indicated this polymer can be relevant in the pharmaceutical industry for production of ***,our research group reported that PCG can be used as a diluent for tablets produced by direct *** (Nys) is the drug of first choice for treatment of oral candidiasis,in the form of a *** treatment consists of up to six daily doses of a suspension of nys at 500,000 IU,causing low therapeutic adhesion by *** objective of this study was to investigate the behavior of PCG together with nys and other excipients (flavoring agents and lubricating agent) for future manufacture of mucoadhesive buccal tablets by direct *** that purpose,we performed pre-formulation tests (FTIR,TGA,XRD,solubility,pH,granulometry,swelling degree and powder flow) with physical mixtures of the drug and *** results were excellent,demonstrating that PCG is a polymer with potential for this type of application.

关键词： Purified cashew gum nystatin mucoadhesive buccal tablets

P53 protein expression and cell viability in irradiated peripheral blood mononuclear cells as bioindicators of radiosensitivity

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Journal of Biophysical Chemistry 2011年第2期2卷 63-67页

作者： Mariana Brayner Cavalcanti ana paula Galvao da Silva Rafael de Freitas e Silva Ademir Amaral Grupo de Estudos em Radioprotecao e Radioecologia(GERAR) Laboratorio de Modelagem e Biodosimetria Aplicada(LAMBDA)Departamento de Energia NuclearUniversidade Federal de PernambucoCidade UniversitariaPernambucoBrazil anaptysbio Incorporation Cell BiologySan DiegoUSA

Cellular radiosensitivity is directly correlated with the mechanism of DNA repair, in which p53 protein plays a major role. In this context, this study correlated cell death with p53 expression in lymphocytes irradiated in vitro with different doses of gammaradiation. For this, peripheral blood samples were collected from 10 healthy subjects. Each sample was divided in aliquots and, separately, irradiated with doses of 0,5;2 and 4 Gy. After this, peripheral blood mononuclear cells (PBMCs) were isolated and cultivated during 72 hours in 5% CO2 at 37oC without mitogen stimulation. The expression of p53 protein was evaluated by flow cytometry. In parallel, cell viability was determined by trypan blue staining. Statistical analysis was performed us-ing analysis of variance (ANOVA), differences were considered as statistically significant when p < 0.05. The results showed an increase of p53 expression with the absorbed dose, which was proportional to cell death, suggesting that p53 can be used as bioindicator of individual radiosensitivity.

关键词： P53 Protein Radiosensitivity Lymphocytes Flow Cytometry Cell Viability

Land-use change and windstorms legacies drove the recolonization dynamics of laurel forests in Tenerife, Canary islands

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Forest Ecosystems 2023年第1期10卷 113-124页

作者： Vicente Rozas Maria A.Garcia-Lopez Jose M.Olano Gabriel Sanguesa-Barreda Miguel Garcia-Hidalgo Susana Gomez-Gonzalez Roberto Lopez-Rubio Jose M.Fernandez-Palacios Ignacio Garcia-Gonzalez Laura Lozano-Lopez paula Garcia-Gonzalez ana I.Garcia-Cervigon EiFAB-iuFOR Universidad de ValladolidCampus Duques de Soria42004SoriaSpain Departamento de Biologia-IVAGRO Universidad de CadizCampus Rio San Pedro11510Puerto RealSpain Center for Climate and Resilience Research(CR)2 Blanco Encalada 20028370449SantiagoChile Area de Biodiversidad y Conservacion Universidad Rey Juan Carlosc/Tulipan s/n28933MostolesSpain Grupo de Ecologia y Biogeografia Insular Universidad de La Laguna38206La LagunaTenerifeSpain BIOAPLIC Departamento de BotanicaEscola Politecnica Superior de EnxenariaCampus TerraUniversidade de Santiago de CompostelaLugo27002Spain

Laurel forests are quite relevant for biodiversity conservation and are among the island ecosystems most severely damaged by human *** the past,Canary laurel forests have been greatly altered by logging,livestock and *** remains of laurel forests are currently protected in the Canary Islands(Spain).However,we miss basic information needed for their restoration and adaptive management,such as tree longevity,growth potential and responsiveness to natural and anthropogenic *** dendrochronological methods,we studied how forest dynamic is related to land-use change and windstorms in two well-preserved laurel forests on Tenerife *** cores were collected from over 80 trees per stand at three stands per *** used ring-width series to estimate tree ages and calculate annual basal area increments(BAI),cumulative diameter increases,and changes indicative of released and suppressed *** tree species were found in all stands,with Laurus novocanariensis,Ilex canariensis and Morella faya being the most common *** some individuals were over 100 years old,61.8%-88.9% of the trees per stand established between 1940 and 1970,coinciding with a post-war period of land abandonment,rural exodus and the onset of a tourism *** trees have shown growth rates larger than 1 cm diameter per year and most species have had increasing BAI trends over the past *** growth releases occurred after windstorms at both sites,but the effects of windstorms were site-dependent,with the 1958 storm affecting mainly the eastern tip of the island(anaga massif)and the 1991 storm the western tip(Teno massif).Given the great ability of laurel forest trees to establish after land use cessation and to increase growth after local disturbances such as windstorms,passive restoration may be sufficient to regenerate this habitat in currently degraded areas.

关键词： Canary islands Dendroecology Disturbance Forest structure Macaronesia Management cessation Tree rings

NT157 has antineoplastic effects and inhibits IRS1/2 and STAT3/5 in JAK2^(V617F)-positive myeloproliferative neoplasm cells

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Signal Transduction and Targeted Therapy 2020年第1期5卷 2440-2449页

作者： Bruna Alves Fenerich Jaqueline Cristina Fernandes ana paula Nunes Rodrigues Alves Juan Luiz Coelho-Silva Renata Scopim-Ribeiro Priscila Santos Scheucher Christopher A.Eide Cristina E.Tognon Brian J.Druker Eduardo Magalhães Rego João Agostinho Machado-Neto Fabiola Traina Department of Medical Images Hematologyand Clinical OncologyUniversity of Sao Paulo at Ribeirao Preto Medical SchoolRibeirao PretoSao PauloBrazil Center for Cell-Based Therapy Sao Paulo Research FoundationRibeirao PretoSao PauloBrazil Knight Cancer Institute Oregon Health&Science UniversityPortlandORUSA Howard Hughes Medical Institute PortlandORUSA Department of Internal Medicine University of Sao Paulo Medical SchoolSao PauloBrazil Department of Pharmacology Institute of Biomedical Sciences of the University of Sao PauloSao PauloBrazil

Recent data indicate that IGF1R/IRS signaling is a potential therapeutic target in BCR-ABL1-negative myeloproliferative neoplasms(MPN);in this pathway,IRS2 is involved in the malignant transformation induced by JAK2^(V617F),and upregulation of IGF1R signaling induces the MPN ***157,a synthetic compound designed as an IGF1R-IRS1/2 inhibitor,has been shown to induce antineoplastic effects in solid ***,we aimed to characterize the molecular and cellular effects of NT157 in JAK2^(V617F)positive MPN cell lines(HEL and SET2)and primary patient hematopoietic *** JAK2^(V617F)cell lines,NT157 decreased cell viability,clonogenicity,and cell proliferation,resulting in increases in apoptosis and cell cycle arrest in the G2/M phase(p<0.05).NT157 treatment inhibited IRS1/2,JAK2/STAT,and NFκB signaling,and it activated the AP-1 complex,downregulated four oncogenes(CCND1,MYB,WT1,and NFKB1),and upregulated three apoptotic-related genes(CDKN1A,FOS,and JUN)(p<0.05).NT157 induced genotoxic stress in a JAK2/STAT-independent ***157 inhibited erythropoietin-independent colony formation in cells from polycythemia vera patients(p<0.05).These findings further elucidate the mechanism of NT157 action in a MPN context and suggest that targeting IRS1/2 proteins may represent a promising therapeutic strategy for MPN.

关键词： JAK2 IGF1R neoplasms STAT3