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Function, kinetic properties, crystallization, and regulation of microbial malate dehydrogenase

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第4期17卷 247-261页

作者： Toshiko TAKAHASHI-INIGUEZ Nelly ABURTO-RODRIGUEZ ana laura vilchis-gonzalez Maria Elena FLORES Departamento de Biologla Molecular y Biotecnologia lnstituto de Investigaciones BiomddicasUniversidad Nacional Autonoma de MexicoMexicoD.F.04510Mexico

Malate dehydrogenase （MDH） is an enzyme widely distributed among living organisms and is a key protein in the central oxidative pathway. It catalyzes the interconversion between malate and oxaloacetate using NAD＋ or NADP＊ as a cofactor. Surprisingly, this enzyme has been extensively studied in eukaryotes but there are few reports about this enzyme in prokaryotes. It is necessary to review the relevant information to gain a better understanding of the function of this enzyme. Our review of the data generated from studies in bacteria shows much diversity in their molecular properties, including weight, oligomeric states, cofactor and substrate binding affinities, as well as differences in the direction of the enzymatic reaction. Furthermore, due to the importance of its function, the transcription and activity of this enzyme are rigorously regulated. Crystal structures of MDH from different bacterial sources led to the identification of the regions involved in substrate and cofactor binding and the residues important for the dimer-dimer interface. This structural information allows one to make direct modifications to improve the enzyme catalysis by increasing its activity, cofactor binding capacity, substrate specificity, and thermostability. A comparative analysis of the phylogenetic reconstruction of MDH reveals interesting facts about its evolutionary history, dividing this superfamily of proteins into two principle clades and establishing relationships between MDHs from different cellular compartments from archaea, bacteria, and eukaryotes.

关键词： Malate dehydrogenase Carbon metabolism Tricarboxylic acid cycle

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Properdin deficiency associated with systemic meningococcal disease due to a novel ***337Arg pathogenic variant

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Genes & Diseases 2024年第6期11卷 43-46页

作者： laura gonzalez-Sanchez ana Mei Agudo Anne Van Den Rym Maria Isabel Begiristain Alazne Saizar Rebeca Perez de Diego Pilar Nozal Alberto Lopez-Lera Margarita Lopez-Trascasa Fernando Corvillo Complement Research Group Hospital La Paz Institute for Health Research(ldiPAZ)La Paz University HospitalMadrid 28046Spain Center for Biomedical Network Research on Rare Diseases MadridSpain Laboratory of Immunogenetics of Human Diseases IdiPAz Institute for Health ResearchLa Paz University HospitalMadrid 28046Spain Ormaiztegui Clinic Guipuzcoa 20216Spain Immunology Unit La Paz University HospitalMadrid 28046Spain Departamento de Medicina Universidad Autonoma de MadridMadrid28029Spain

Properdin(FP)is a soluble glycoprotein with a key role in the regulation of the alternative pathway(AP)of the complement system.1 Neutrophils are the main source of FP,although monocytes,bone marrow progenitors,and T cells also synthesize this complement *** is codified by the gene CFP,located at Xp11.23-p11.3,and formed by 10 exons,of which exons 2 to 10 are translated conforming six thrombospondin type 1 repeats(TSR1-6).

关键词： Xp11.2 glycoprotein progenitor

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Land-use change and windstorms legacies drove the recolonization dynamics of laurel forests in Tenerife, Canary islands

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Forest Ecosystems 2023年第1期10卷 113-124页

作者： Vicente Rozas Maria A.Garcia-Lopez Jose M.Olano Gabriel Sanguesa-Barreda Miguel Garcia-Hidalgo Susana Gomez-gonzalez Roberto Lopez-Rubio Jose M.Fernandez-Palacios Ignacio Garcia-gonzalez laura Lozano-Lopez Paula Garcia-gonzalez ana I.Garcia-Cervigon EiFAB-iuFOR Universidad de ValladolidCampus Duques de Soria42004SoriaSpain Departamento de Biologia-IVAGRO Universidad de CadizCampus Rio San Pedro11510Puerto RealSpain Center for Climate and Resilience Research(CR)2 Blanco Encalada 20028370449SantiagoChile Area de Biodiversidad y Conservacion Universidad Rey Juan Carlosc/Tulipan s/n28933MostolesSpain Grupo de Ecologia y Biogeografia Insular Universidad de La Laguna38206La LagunaTenerifeSpain BIOAPLIC Departamento de BotanicaEscola Politecnica Superior de EnxenariaCampus TerraUniversidade de Santiago de CompostelaLugo27002Spain

Laurel forests are quite relevant for biodiversity conservation and are among the island ecosystems most severely damaged by human *** the past,Canary laurel forests have been greatly altered by logging,livestock and *** remains of laurel forests are currently protected in the Canary Islands(Spain).However,we miss basic information needed for their restoration and adaptive management,such as tree longevity,growth potential and responsiveness to natural and anthropogenic *** dendrochronological methods,we studied how forest dynamic is related to land-use change and windstorms in two well-preserved laurel forests on Tenerife *** cores were collected from over 80 trees per stand at three stands per *** used ring-width series to estimate tree ages and calculate annual basal area increments(BAI),cumulative diameter increases,and changes indicative of released and suppressed *** tree species were found in all stands,with Laurus novocanariensis,Ilex canariensis and Morella faya being the most common *** some individuals were over 100 years old,61.8%-88.9% of the trees per stand established between 1940 and 1970,coinciding with a post-war period of land abandonment,rural exodus and the onset of a tourism *** trees have shown growth rates larger than 1 cm diameter per year and most species have had increasing BAI trends over the past *** growth releases occurred after windstorms at both sites,but the effects of windstorms were site-dependent,with the 1958 storm affecting mainly the eastern tip of the island(anaga massif)and the 1991 storm the western tip(Teno massif).Given the great ability of laurel forest trees to establish after land use cessation and to increase growth after local disturbances such as windstorms,passive restoration may be sufficient to regenerate this habitat in currently degraded areas.

关键词： Canary islands Dendroecology Disturbance Forest structure Macaronesia Management cessation Tree rings

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Knowledge and preventive measures practiced by junior high school students from Mexico City regarding influenza A (H1N1)

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Health 2012年第10期4卷 995-999页

作者： Veronica Cruz-Licea Fernando gonzalez-Dominguez laura Vargas-Parada Monica Hernandez-Riquelme ana Flisser Departamento de Salud Publica Facultad de MedicinaUniversidad Nacional Autonoma de MexicoMexico CityMexico Direccion de Ensenanza Hospital General“Dr.Manuel Gea Gonzalez”Secretaria de SaludMexico CityMexico Departamento de Biologia Facultad de CienciasUniversidad Nacional Autonoma de MexicoMexico CityMexico Direccion General de Innovacion y Fortalecimiento Academico Administracion Federal de Servicios Educativos en el Distrito FederalSecretaria de Educacion PublicaMexico CityMexico Departamento de Microbiologia y Parasitologia Facultad de MedicinaUniversidad Nacional Autónoma de MexicoMexico CityMexico

Background: Influenza A (H1N1) is the most recent pandemic disease that has affected the human population. Objective: To evaluate knowledge and preventive measures related with this disease one year after the epidemic of Mexico- that took place in 2009. Material and methods: An epidemiologic survey regarding influenza A (H1N1) was conducted in June 2010 among 2541 students from the second grade of all public high schools in a borough in Mexico City. The questionnaire included items on the knowledge of the disease and practice of preventive measures. Results: Most students obtained the information from television, half of them from parents and only one fifth from teachers;72% of the participants had a favorable knowledge about the disease and the measures to avoid being infected. However, only 37% practiced such preventive measures. Conclusion: Knowledge has a positive influence on practices towards health. Parents and teachers have an important role in health education, thus efforts should be directed to involve them more intensely in health education.

关键词： Alcohol Gel Epidemiologic Survey Hand Washing Health Behavior Influenza A (H1N1) Outbreak Mexico City

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Comparative Transcriptomic analysis of Salt Adaptation in Roots of Contrasting Medicago truncatula Genotypes

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Molecular Plant 2012年第5期5卷 1068-1081页

作者： Ons Zahaf Sandrine Blanchet Axel de Zelicourt Benoit Alunni Julie Plet Carole Laffont laura de Lorenzo Sandrine Imbeaud Jean-Laurent lchante Anouck Diet Mounawer Badri ana Zabalza Esther M. gonzalez Herve Delacroix Veronique Gruber Florian Frugier Martin Crespi Institut des Sciences du Vegetal (ISV) CNRS 91198 Gif-sur-Yvette France Universite Paris Diderot Paris 7 75205 Paris Cedex 13 France Departamento de Microbiologia y Parasitologia Universidad de Sevilla 41012 Sevilla Spain Present address: Centro Nacional de Biotecnologia (CNB) CSIC 28049 Madrid Spain Centre de Genetique Moleculaire (CGM) CNRS Gif/Orsay DNA Microarray Platform (GODMAP) Universite Paris-Sud 11 91190 Gif-sur-Yvette France Present address: Genomique Fonctionnelle des tumeurs solides INSERM IUH Universit~ Paris Descartes 75010 Paris France Laboratory of Legumes Centre of Biotechnology of Borj Cedria BP 901 2050 Hammam-Lif Tunisia Departamento de Ciencias del Medio Natural Universidad P~blica de Navarra 31006 Pamplona Spain

Evolutionary diversity can be driven by the interaction of plants with different environments. Molecular bases involved in ecological adaptations to abiotic constraints can be explored using genomic tools. Legumes are major crops worldwide and soil salinity is a main stress affecting yield in these plants. We analyzed in the Medicago truncatula legume the root transcriptome of two genotypes having contrasting responses to salt stress： TN1.11, sampled in a salty Tunisian soil, and the reference Jemalong A17 genotype. TN1.11 plants show increased root growth under salt stress as well as a differential accumulation of sodium ions when compared to A17. Transcriptomic analysis revealed specific gene clusters preferentially regulated by salt in root apices of TN1.11, notably those related to the auxin pathway and to changes in histone variant isoforms. Many genes encoding transcription factors （TFs） were also differentially regulated between the two genotypes in response to salt. Among those selected for functional studies, overexpression in roots of the A17 ge- notype of the bHLH-type TF most differentially regulated between genotypes improved significantly root growth under salt stress. Despite the global complexity of the differential transcriptional responses, we propose that an increase in this bHLH TF expression may be linked to the adaptation of M. truncatula to saline soil environments.

关键词： root architecture abiotic stress legume transcriptome bHLH.

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Health Status of Urban Medium-Low-Income Elderly at the Community

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Open Journal of Endocrine and Metabolic Diseases 2018年第3期8卷 105-116页

作者： Lilia Cárdenas-Ibarra Jesús Z. Villarreal-Pérez Raúl Fernando Gutiérrez Herrera Perla Alejandra Mellado-Urbina ana laura Turner-Llaguno Adolfo Montemayor-Alatorre José Carlos Lira-Castillo Fernando J. Lavalle González Endocrinology Service Dr. José E. González University Hospital Universidad Autónoma de Nuevo León (UANL) San Nicolás de los Garza Mexico Family Practice Department Dr. Jose E. Gonzalez University Hospital UANL San Nicolás de los Garza Mexico Medical School UANL San Nicolás de los Garza Mexico

Background: Nonagenarians are uncommon in our population. The study aim was to profile physical mobility, declared health problems, hypertension and diabetes frequency, awareness, treated and good control in urban low-income elders. Method: The study had cross-sectional design in population sample. Subjects were urban men and women older than 59 years at the medium low income stratum at a developing country. Survey was carried out from October 2008 to October 2009. All elder dwellers in randomly selected houses were surveyed;clinical exam after consent. Good Control: Systolic/Diastolic Results: There were 162 elders, 53 (32.7%) were male, 99 (61.1%) were younger than 70 years. Forty nine (30.2%) had impaired mobility;among them 20 (55.6%) were bed or home restricted, and 29 (44.4%) needed help to go out. Unstable health condition was found in 36 (22.2%), with significantly more men 19 (52.8%) vs 34 (27.0%), X2 = 8.5, df1, P 0.05. The frequency, proportions for: known diagnosis, on treatment, and good control were 75 (69.4%), 55 (73.3%), 45 (60.0%) and 12 of 45 (26.7%) for hypertension;53 (49.1%), 47 (88.7%), 42 (79.2%) and 9 of 42 (21.4%) for diabetes. The most frequent unstable conditions were cardiovascular. Conclusions: Data suggest unstable health no association with the oldest old. The small proportion in good control of Diabetes and/or hypertension could explain the large frequency of elders with unstable health and prevention of reaching nonagenarian age.

关键词： Community Health Elderly Awareness Treatment and Control of Hypertension and Diabetes Mellitus Type 2

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