检索结果-南通市图书馆

Endogenous prostaglandin d2synthesis inhibits e-selectin generation in human umbilical vein endothelial cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Health 2011年第5期3卷 304-311页

作者： Hideyuki Negoro Hiroyuki kobayashi Yoshio Uehara 不详

We examined the role of prostaglandin d2 (PGd2) in the formation of E-selectin following inter-leukin-1 (IL-1) stimulation in human umbilical vein endothelial cells (HUVEC) transfected with lipocaline-type PGd2 synthase (L-PGdS) genes. HUVEC were isolated from human umbilical vein and incubated with 20 U/mL IL-1 and various concentrations of authentic PGd2. The isolated HUVEC were also transfected with L-PGdS genes by electroporation. The L-PGdS-transfected HUVEC were used to investigate the role of endogenous PGd2 in IL-1-stimulated E-selectin biosynthesis. We also used an anti-PGd2 antibody to examine whether an intracrine mechanism was involved in E-selectin production. PGd2 and E-selectin levels were determined by radio-immunoassay and enzyme- immunoassay, respectively. E-selectin mRNA was assessed by real-time RT-PCR. IL-1-stimulated E-selectin production by HUVEC was dose-dependently inhibited by authentic PGd2 at concentrations greater than 10–6 mol/L. L-PGdS gene-transfected HUVEC produced more PGd2 than HUVEC transfected with the reporter gene alone. IL-1 induced increases in E-selectin production in HUVEC transfected with the reporter genes alone. However, this effect was significantly attenuated in the case of IL-1 stimulation of HUVEC trans-fected with L-PGdS genes, and accompanied by an apparent suppression of E-selectin mRNA expression. Neutralization of extracellular PGd2 by anti-PGd2- specific antibody influenced neither E-selectin mRNA expression nor E-selectin biosynthesis. HUVEC transfected with L-PGdS genes showed increased PGd2 synthesis. This increase was associated with attenuation of both E-selectin generation and E-selectin mRNA expression. The results suggest that endogenous PGd2 decreases E-selectin synthesis and E-selectin mRNA expression, probably through an intracrine mechanism.

关键词： Prostaglandin E-selectin PGdS Endothelial Cell

Characterization of Full-WdM-Band Photodiode Modules Using Raman Amplifiers in the U Band at 40 Gb/s

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

光学学报 2003年第S1期23卷 447-448页

作者： Morio Wada Toshimasa Umezawa Takahiro Kudou Takashi Mogi Shinji Iio Shinji kobayashi Tsuyoshi Yakihara Akira Miura Corporate R&d Center Yokogawa Electric Co. JapanCorporate R&D Center Yokogawa Electric Co. JapanCorporate R&D Center Yokogawa Electric Co. JapanCorporate R&D Center Yokogawa Electric Co. JapanCorporate R&D Center Yokogawa Electric Co. JapanCorporate R&D Center Yokogawa Electric Co. JapanCorporate R&D Center Yokogawa Electric Co. JapanCorporate R&D Center Yokogawa Electric Co. Japan

We present the characteristics of a full-WdM-band photodiode module in 40 Gb/s U-band operation using a Raman amplifier. This module is suitable for wide-band transmission systems with the additional new channels in t... 详细信息

关键词： WdM for with Characterization of Full-WdM-Band Photodiode Modules Using Raman Amplifiers in the U Band at 40 Gb/s of in

厚靶d(d,n)~3He反应加速器中子源的产额、能谱和角分布(英文)

在线全文

学校读者我要写书评

暂无评论

计算物理 2008年第6期25卷 744-748页

作者：姚泽恩杜洪新谭新建张宇 Tooru kobayashi Gerard Bengua 兰州大学核科学与技术学院甘肃兰州730000 日本京都大学反应堆研究所大阪590-0494

给出一种计算氘钛厚靶d(d,n)3He反应加速器中子源的产额、能谱和角分布的方法,并发展了一个计算机模拟程序,程序能够计算氘束流能量小于1.0 MeV的中子源的产额、能谱和角分布.计算时使用推荐的d(d,n)3He反应截面数据和来自SRIM-2003程... 详细信息

给出一种计算氘钛厚靶d(d,n)3He反应加速器中子源的产额、能谱和角分布的方法,并发展了一个计算机模拟程序,程序能够计算氘束流能量小于1.0 MeV的中子源的产额、能谱和角分布.计算时使用推荐的d(d,n)3He反应截面数据和来自SRIM-2003程序的氘在氘钛靶中的阻止本领数据.给出一些典型计算结果,包括中子积分产额、中子能谱和角分布.

关键词： d(d,n)^3He反应 Tidx厚靶中子产额中子能谱中子角分布

Numerical modeling for the coupled thermo-mechanical processes and spalling phenomena in sp Pillar Stability Experiment (APSE)

在线全文

学校读者我要写书评

暂无评论

Journal of Rock Mechanics and Geotechnical Engineering 2013年第1期5卷 58-72页

作者： T.Koyama M.Chijimatsu H.Shimizu S.Nakama T.Fujita A.kobayashi Y.Ohnishi department of Urban Management Kyoto University Hazama Corporation Institute of Fluid Science Tohoku University Japan Atomic Energy Agency (JAEA) Faculty of Environmental and Urban Engineering Kansai University Kyoto University

In this paper, the coupled thermo-mechanical （TM） processes in the AEspoe Pillar Stability Experiment （APSE） carried out by the Swedish Nuclear Fuel and Waste Management Company （SKB） were simulated using both continuum and discontinuum based numerical methods. Two-dimensional （2d） and three- dimensional （3d） finite element method （FEM） and 2d distinct element method （dEM） with particles were used. The main objective for the large scale in situ experiment is to investigate the yielding strength of crystalline rock and the formation of the excavation disturbed/damaged zone （EdZ） during excavation of two boreholes, pressurizing of one of the boreholes and heating. For the dEM simulations, the heat flow algorithm was newly introduced into the original code. The calculated stress, displacement and temperature distributions were compared with the ones obtained from in situ measurements and FEM simulations. A parametric study for initial microcracks was also performed to reproduce the spalling phenomena observed in the APSE.

关键词： Coupled thermo-mechanical (TM)processesAspoe Pillar Stability Experiment (APSE)Excavation disturbed/damaged zone (EdZ)Finite element method (FEM)distinct element method (dEM)

Tongue-like Barrett's esophagus is associated with gastroesophageal reflux disease

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第26期14卷 4196-4203页

作者： Hatsushi Yamagishi Tomoyuki Koike Shuichi Ohara Shigeyuki kobayashi Ken Ariizumi Yasuhiko Abe Katsunori Iijima Akira Imatani Yoshifumi Inomata Katsuaki Kato daisuke Shibuya Shigemitsu Aida Tooru Shimosegawa division of Gastroenterology Tohoku University Graduate School of MedicineSendai 980-8574Japan Miyagi Cancer Society Sendai980-0011Japan department of Gastroenterlogy Sendai City HospitalSendai 984-8501Japan

AIM:To test this hypothesis of barrett esophagus (BE) classified into two types and to further determine if there was any correlation between the shape of endoscopically suspected esophageal metaplasia (ESEM), prevalence of reflux esophagitis (RE) and heartburn. METHOdS: A total of 6504 Japanese who underwent endoscopy for their annual stomach check-up were enrolled in this study. BE was detected without histological confirmation that is ESEM. We originally classified cases of ESEM into 3 types based on its shape: Tongue-like (T type), dome-like (d type) and Wave-like (W type) ESEM. The respective subjects were prospectively asked to complete questionnaires concerning the symptoms of heartburn, dysphagia, and abdominal pain for a one-month period. RESULTS: ESEM was observed in 10.3% of 6504 subjects (ESEM d with female subjects. Statistical analysis showed that the prevalence of heartburn and RE were significantly higher in the T type ESEM than in the W type ESEM (P d with reflux symptoms and RE whereas the W type ESEM was not associated with GERd.

关键词： Tongue-like endoscopically suspectedesophageal metaplasia dome-like endoscopicallysuspected esophageal metaplasia Wave-likeendoscopically suspected esophageal metaplasia Gastroesophageal reflux disease

A High-Precision US-Guided Robot-Assisted HIFU Treatment System for Breast Cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering 2018年第5期4卷 702-713页

作者： Tianhan Tang Takashi Azuma Toshihide Iwahashi Hideki Takeuchi Etsuko kobayashi Ichiro Sakuma Graduate School of Engineering The University of Tokyo Tokyo 113-8656 Japan Graduate School of Medicine The University of Tokyo Tokyo 113-8654 Japan

Breast cancer is the most commonly diagnosed cancer in women. A strong treatment candidate is high- intensity focused ultrasound （HIFU）, a non-invasive therapeutic method that has already demonstrated its promise. To improve the precision and lower the cost of HIFU treatment, our group has developed an ultrasound （US）-guided, five-degree-of-freedom （dOF）, robot-assisted HIFU system. We constructed a fully functional prototype enabling easy three-dimensional （3d） US image reconstruction, target seg-mentation, treatment path generation, and automatic HIFU irradiation. The position was calibrated using a wire phantom and the coagulated area was assessed on heterogeneous tissue phantoms. Under the US guidance, the centroids of the HIFU-ahlated area deviated by less than 2 mm from the planned treatment region. The overshoot around the planned region was well below the tolerance of clinical usage. Our system is considered to he sufficiently accurate for breast cancer treatment.

关键词： HIFU Breast cancer US-guided navigation Medical robotics Accuracy evaluation

Purification of dengue VirusParticles by One-Step Ceramic Hydroxyapatite Chromatography

在线全文

学校读者我要写书评

暂无评论

World Journal of Vaccines 2012年第3期2卷 155-160页

作者： Yae Kurosawa Maiko Saito Shintaro kobayashi Tsuneo Okuyama Protein Technos Institute Kanagawa Japan R&d department PENTAX New Ceramics Division HOYA Corporation Tokyo Japan

dengue virus type 2 ThNH7/93 retained infectious activity after purification by ceramic hydroxyapatite chromatogra-phy. dengue virus type 2 culture fluid was loaded onto the ceramic hydroxyapatite column and eluted with a linear gradient of sodium phosphate buffer. Culture fluid and protein contaminants derived from host cells were eluted initially, followed by elutions of dsdNA, and then dengue virus type 2. The recoveries of dengue virus type 2 were 64 ± 14% (n = 11) in the hemagglutination (HA) test and 60% (n = 2) determined by focus assay for viral infectivity. This protocol was highly reproducible, simple, rapid, and appears applicable to other virus species such as influenza virus, Japanese encephalitis virus and adenovirus.

关键词： Ceramic Hydroxyapatite Virus Purification dengue Virus Virus Particle Virion

Safety and Efficacy of Combination Therapy with Insulin Glargine and Oral Hypoglycaemic Agents Including dPP-4 Inhibitors in Japanese T2dM Patients: ALOHA 2 Study, a Post-Marketing Surveillance for Lantus®

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of diabetes Mellitus 2014年第4期4卷 273-289页

作者： Masayuki kobayashi Shoko Tsukube Yukio Ikeda Yujin Shuto Sanofi K.K. Tokyo Japan Sanofi K.K. Tokyo Japanc

Aims: In the Add-on Lantus®?to Oral Hypoglycaemic Agents 2 (ALOHA 2) Study in Japanese adults with type 2 diabetes mellitus (T2dM), data on the safety and efficacy of combination therapy with insulin glargine (Lantus®) and oral anti-hyperglycaemic drugs (OAds) including dipeptidyl peptidase-4 (dPP-4) inhibitors in a real-life setting were collected and analyzed. Methods: This postmarketing surveillance was a prospective, observational, 24-week study that complied with the pharmaceutical affairs law and the ministerial ordinance of “Good Post-Marketing Study Practice (GPSP)” in Japan. Safety, efficacy and patient-reported outcomes (PROs);patients’ satisfaction with treatment (dTSQs and dTSQc) and patients’ self-reported health (EQ-5d and EQ-VAS) of combination therapy of insulin glargine and OAds were evaluated. Results: A total of 2,630 patients were enrolled. Of the 2,602 patients in the safety analysis population, 161 patients experienced 175 cases of adverse drug reactions, and the major adverse drug reaction was hypoglycaemia (140 patients, 5.38%). Out of those with hypoglycaemia, 11 patients (0.42%) had severe hypoglycaemia and the incidence rate (episodes per patient-year) was 0.019. Basal supported oral therapy (BOT) with insulin glargine substantially reduced the HbA1c, FPG and 2 hour-PPG levels for 24 weeks by -1.61%, -54.4 mg/dL and -74.5 mg/dL respectively. The mean weight was increased, however the change was +0.50 kg. In addition, the treatment satisfaction scores of dTSQs (mean treatment satisfaction score increased 3.6 from baseline to last observation) and dTSQc, EQ-5d index scores and EQ-VAS scores were significantly improved. Conclusion: Insulin glargine and OAds combination therapy was suggested to be effective and well tolerated. Patients’ satisfaction with treatment and their self-reported health improved in spite of the addition of injections to oral agents. The combination therapy of insulin glargine and OAds including dPP-4 inhibitors

关键词： ALOHA 2 Insulin Glargine Patient-Reported Outcomes Post-Marketing Surveillance Study Type 2 diabetes

~2H(d,n)~3He核反应中子注量的伴随粒子法测量

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

核电子学与探测技术 2002年第3期22卷 200-203页

作者：叶邦角 H.Nanjyo T.kobayashi 中国科学技术大学近代物理系安徽合肥230027 ICEPP University of Tokyo Hongo ICEPP University of Tokyo Hongo

2 H(d,n) 3 He核反应单能中子源广泛应用于几 Me V中子的散射和极化实验。采用伴随粒子法测量中子注量 ,用 Si半导体探测器测量 3 He粒子 ,用 0 .8μm Al箔来屏蔽散射的 d束 ,系统可很好地分辨 3 He、d、T和 p,可测 d+束能量到 16 5 ke... 详细信息

2 H(d,n) 3 He核反应单能中子源广泛应用于几 Me V中子的散射和极化实验。采用伴随粒子法测量中子注量 ,用 Si半导体探测器测量 3 He粒子 ,用 0 .8μm Al箔来屏蔽散射的 d束 ,系统可很好地分辨 3 He、d、T和 p,可测 d+束能量到 16 5 ke V,测量结果与用 NE2 13探测器的结果相比较 ,一致性好于97%。

关键词：测量核反应中子注量伴随粒子法单能中子源氦3 氢2