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Molecular identification of sex in Hippophae rhamnoides L. using isozyme and RAPD markers

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Forestry Studies in China 2010年第2期12卷 62-66页

作者： amit sharma Gaurav ZINTA Satender RANA Poonam SHIRKOT Department of Biotechnology Dr. Yashwant Singh Parmar University of Horticulture & Forestry

In many dioecious plants, gender affects economic value, breeding schemes and opportunities for commercial harvests. Hippophae rhamnoides L. is a dioecious plant species in which female genotypes are commercially preferred over male genotypes. Its berries have rich medicinal, nutritional and pharmaceutical properties because of their large amounts of vitamins, essential oils, proteins, fatty acids, free amino acids and flavanoids. Primary limitation for breeding H. rhamnoides L. is its dioecious nature, since gender cannot be identified by traditional methods. Therefore, some reliable and quick methods need to be developed. This commu- nication deals with the development of isozyme and RAPD markers for early sex identification in this dioecious tree. The isozyme analysis was conducted with four enzyme systems, viz. peroxidase, esterase, malate dehydrogenase and catalase. The peroxidase enzyme system produced a female specific sex marker, which successfully differentiated between the staminate and pistillate geno- types ofH. rhamnoides L. Thirty five random decamer primers were used in our study and one male sex linked marker was identified. OPD-20 （5＇-ACTTCGCCAC-3＇） displayed a band at 911 bp that expressed polymorphism between male and female genotypes. The staminate and pistillate genotypes could be distinguished using RAPD marker OPD-209n. These results revealed the immense poten- tial of peroxidase isozyme patterns and RAPD as genetic markers for sex identification in H. rhamnoides L.

关键词： Hippophae rhamnoides L. sex markers isozyme RAPD dioecism

Impact of vericiguat on heart failure with reduced ejection fraction:a review

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Global Health Journal 2023年第3期7卷 123-129页

作者： Manisha Vohra Mohammad Amir Ian Osoro amit sharma Ranjeet Kumar Department of Pharmacy Practice ISF College of PharmacyMogaPunjab 142001India

Introduction:Heart failure is a major public health issue with a prevalence of about 26 million people *** nitric oxide availability,lower soluble guanylate cyclase(sGC)activity,and decreased cyclic guanosine monophosphate(cGMP)production are the causes of HF's *** prescribed under the brand name Verquvo was approved by *** and Drug Administration(FDA)in January *** is a novel agent and the first sGC stimulator which helps to treat patients suffering from heart failure with reduced ejection fraction(HFrEF).Objective:The mechanism of action(cGMP pathway)of vericiguat,its clinical trials,its use in the treatment of heart failure,and its possible future aspects in therapeutic recommendations are all covered in this *** will also raise awareness amongst healthcare professionals about the pharmacokinetic and pharmacodynamic parameters,dosing,administration,and drug-related problems of this new ***:Various databases for drug review were used in this review like PubMed,Medline,Google scholar,Drug bank,***,Medscape,and European society of cardiology guidelines.A total of 58 articles were screened out of which 39 articles were included in this ***:This review discusses vericiguat's mechanism of action(cGMP pathway),clinical studies,application in the treatment of heart failure,and potential future considerations in therapeutic *** will also educate healthcare professionals about the new drug's pharmacokinetics and pharmacodynamics,dose,administration,and drug-related ***:After hospitalization for HFrEF,the 5-year survival rate is just 25%,and disease morbidity and death are stil *** adjunctive therapy for individuals with heart failure and a low ejection fraction,vericiguat has a moderate level of ***'s efficacy as an adjunct therapy to different drugs used to cure HF has to be further ***'s safety and dosage in patients w

关键词： Vericiguat Heart failure with reduced ejection fraction Soluble guanylate cyclase stimulator Nitric oxide Cyclicguanosinemonophosphate

The Curation of Genetic Variants: Difficulties and Possible Solutions

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Genomics, Proteomics & Bioinformatics 2012年第6期10卷 317-325页

作者： Kapil Raj Pandey Narendra Maden Barsha Poudel Sailendra Pradhananga amit Kumar sharma Deerwalk Services Department of Microbiology Bangalore University Central Department of Microbiology Tribhuvan University Department of Bioinformatics Wageningen University Department of Biotechnology Kathmandu University

The curation of genetic variants from biomedical articles is required for various clinical and research purposes. Nowadays, establishment of variant databases that include overall information about variants is becoming quite popular. These databases have immense utility, serving as a user-friendly information storehouse of variants for information seekers. While manual curation is the gold standard method for curation of variants, it can turn out to be time-consuming on a large scale thus necessitating the need for automation. Curation of variants described in biomedical literature may not be straightforward mainly due to various nomenclature and expression issues. Though current trends in paper writing on variants is inclined to the standard nomenclature such that variants can easily be retrieved, we have a massive store of variants in the literature that are present as non-standard names and the online search engines that are pre- dominantly used may not be capable of finding them. For effective curation of variants, knowledge about the overall process of curation, nature and types of difficulties in curation, and ways to tackle the difficulties during the task are crucial. Only by effective curation, can variants be correctly interpreted. This paper presents the process and difficulties of curation of genetic variants with possible solutions and suggestions from our work experience in the field including literature support. The paper also highlights aspects of interpretation of genetic variants and the importance of writing papers on variants following standard and retrievable methods.

关键词： Difficulties in curation Automated curation Manual curation Interpretation of variants

The role of drug utilization evaluation in medical sciences

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Global Health Journal 2023年第1期7卷 3-8页

作者： Ruby Gangwar Arvind Kumar Abrar Ahmed Zargar amit sharma Ranjeet Kumar Department of Pharmacy Practice ISF College of PharmacyMogaPunjab 142001India

Background:Drug utilization evaluation(DUE)is defined by the World Health Organization(WHO)and focuses on the medical,social,and economic consequences of pharmaceutical marketing,distribution,prescribing,and usage in *** WHO recommends a physician to every 1000 *** to the recent data from the Health Ministry in 2019,in which 1.16 million doctors are of active population with just 80%,or 0.9 million,*** a result,a ratio of 0.68 doctors for every 1000 people,which is much below as per the WHO *** article describes history,types,WHO guidelines,need and purpose of ***:The main aim of this paper is to provide information about the rational use of medication in outpatient and inpatient department with special emphasis of *** also provides awareness directly to healthcare professionals,researchers,academicians,pharmacist and nurses to reduce the irrationality of ***:The method used to compile this review information gathered from websites,Google scholar,PubMed,Research gate,and studies published on DUE from July 20 to Oct 22 were included as source of ***:We studied more than 35 published study on DUE,that reveals most of the physicians prescribed branded drugs not generic drugs,but WHO prescribing indicator allows to prescribe generic drugs in the hospital pharmacy to maintain better inventory *** may also help to prevent pharmacist misunderstanding during ***:The use of generic prescription names avoids the possibility of medication product duplication and lowers patient *** is important to remember that incorrect medication prescriptions have impact on both patients and their family *** indicators identify irrational prescribing behaviours to make therapy more rational and cost-effective.

关键词： Anatomical therapeutic chemical classification Drug utilization evaluation Prescribing indicators Pregnancy risk classification Rational use of the drug

A Comprehensive Review of Animal Models for Coronaviruses: SARSCoV-2, SARS-CoV, and MERS-CoV

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Virologica Sinica 2020年第3期35卷 290-304页

作者： Ashutosh Singh Rahul Soloman Singh Phulen Sarma Gitika Batra Rupa Joshi Hardeep Kaur amit Raj sharma Ajay Prakash Bikash Medhi Department of Pharmacology Postgraduate Institute of Medical Education and ResearchChandigarh 160012India

The recent outbreak of coronavirus disease(COVID-19) caused by the novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) has already affected a large population of the world. SARS-CoV-2 belongs to the same family of severe acute respiratory syndrome coronavirus(SARS-CoV) and Middle East respiratory syndrome coronavirus(MERSCoV). COVID-19 has a complex pathology involving severe acute respiratory infection, hyper-immune response, and coagulopathy. At present, there is no therapeutic drug or vaccine approved for the disease. There is an urgent need for an ideal animal model that can reflect clinical symptoms and underlying etiopathogenesis similar to COVID-19 patients which can be further used for evaluation of underlying mechanisms, potential vaccines, and therapeutic strategies. The current review provides a paramount insight into the available animal models of SARS-CoV-2, SARS-CoV, and MERS-CoV for the management of the diseases.

关键词： Coronaviruses SARS MERS COVID-19 Animal model

Evaluation of Radarsat-2 quad-pol SAR time-series images for monitoring groundwater irrigation

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International Journal of Digital Earth 2019年第10期12卷 1177-1197页

作者： amit Kumar sharma Laurence Hubert-Moy Buvaneshwari Sriramulu M.Sekhar Laurent Ruiz S.Bandyopadhyay Shiv Mohan Samuel Corgne Univ Rennes UMR LETG CNRSRennesFrance Indo-French Cell for Water Science Indian Institute of ScienceBangaloreIndia Department of Civil Engineering Indian Institute of ScienceBangaloreIndia UMR 1069 SAS INRAAgro-campus OuestRennesFrance Géosciences Environnement Toulouse Universitéde ToulouseCNRSIRDToulouseFrance EOS Indian Space Research OrganizationBangaloreIndia PLANEX Physical Research LaboratoryAhmedabadIndia

Groundwater assists farmers to irrigate crops for fulfilling the crop-water *** agriculture system is characterized by three cropping seasons known as Kharif(monsoon),Rabi(post-monsoon)and summer(pre-monsoon).In tropical countries like India,monitoring cropping practices using optical remote sensing during Kharif and Rabi seasons is constraint due to the cloud cover,which can be well addressed by microwave remote *** the proposed research,the strength of C-band polarimetric Synthetic Aperture Radar(SAR)time series images were evaluated to classify groundwater irrigated croplands for the Kharif and Rabi cropping seasons of the year *** present study was performed in the Berambadi experimental watershed of Kabini river basin,southern peninsular India.A total of fifteen polarimetric variables were estimated includes four backscattering coefficients(HH,HV,VH,VV)and eleven polarimetric indices for all Radarsat-2 SAR *** cumulative temporal sum(seasonal and dual-season)of these parameters was supervised classified using Support Vector Machine(SVM)classifier with intensive ground observation *** results using the best equation(highest accuracy and kappa)shows that the Kharif,Rabi and irrigated double croplands are respectively 9.58 km2(20.6%),16.14 km2(34.7%)and 6.22 km2(13.4%)with a kappa coefficient respectively 0.84,0.74 and 0.94.

关键词： Radarsat-2 Synthetic Aperture Radar Polarimetric Indices Irrigated Cropland Support Vector Machine Classifier Kabini Critical Zone Observatory

Public health progression and related challenges

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Global Health Journal 2023年第1期7卷 1-2,F0002页

作者： amit sharma Ian Osoro Department of Pharmacy Practice ISF College of PharmacyMogaPunjab 142001India

Public health has experienced a significant improvement over the last *** factors are responsible for this:increased scientific knowledge through research and acceptance of health as a public *** more health surveys and publishing the results has been proven to substantially improve public health particularly in developing *** health research remains of utmost importance to society.1-3 Both non-communicable and communicable diseases are on the rise and they are of great concern regardless of age,sex or any other individualistic categorization.

关键词： Public cable publishing

Management of ruptured renal angiomyolipoma with bilateral staghorn calculi

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Asian Journal of Urology 2024年第3期11卷 509-510页

作者： amit sharma Deepak Biswal Kishor Roy R.T.Raghavendra Department of Urology AIIMSRaipurCGIndia

Dear Editor,We present a case of ruptured renal angiomyolipoma(AML)and bilateral renal staghorn *** discuss about the management of this patient by angioembolization,partial nephrectomy,and pyelolithotomy,followed by contralateral interval percutaneous nephrolithotomy(PCNL).Our case is a 40-year-old male who presented with history of dull aching and dragging sensation in bilateral flanks of 6-month *** was admitted and evaluated at other hospital for dizziness,sweating,and loss of *** was found to have bilateral staghorn calculi and left AML and was referred to our institute for further management after initial *** proper written and informed consent was taken from the patient for management as well as publication of the report and images,maintaining the patient’s anonymity.

关键词： admitted sensation renal

Reviewing the insights of SARS-CoV-2:Its epidemiology,pathophysiology,and potential preventive measures in traditional medicinal system

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Clinical Traditional Medicine and Pharmacology 2024年第3期5卷 96-110页

作者： Munish sharma amit Kumar sharma Simran sharma Arvind Kumar sharma Munish sharma Kamal Kant sharma Munit sharma Department of Plant Sciences Central University of Himachal PradeshShahpurKangra176206Himachal PradeshIndia Department of Animal Science Central University of Himachal PradeshShahpur Campus176206KangraIndia Department of Earth and Environmental Sciences Central University of Himachal PradeshShahpur Campus176206KangraIndia Department of Zoology and Environmental Science Gurukula Kangri(Deemed to be University)Haridwar249404UttarakhandIndia Department of Botany and Microbiology Gurukula Kangri(Deemed to be University)Haridwar249404UttarakhandIndia Department of Botany Thakur P.G.College of EducationDhaliara177103DehraHimachal PradeshIndia

Background:The severe acute respiratory syndrome Coronavirus-2 causing COVID-19 viral infection was first reported in late December 2019,spreading swiftly across all over the *** declared it a pandemic by March *** problems emerged worldwide healthcare system and economic burden due to viral *** transmission rate and infectious nature make COVID-19 *** managing outbreak and controlling spread is *** valiant efforts to contain the COVID-19 outbreak,the situation has deteriorated to the point that there were no viable preventive therapies to treat this *** effective treatment for COVID-19 has been a complex and ongoing ***:The aim of this systematic review is to evaluate the structure,epidemiology,pathophysiology,po-tential therapeutic treatments,and preventive measures adopted in Traditional Chinese Medicine(TCM)in the management of Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)***:A systematic methodology has been adopted in this study following PRISMA *** key-words like“COVID-19”“SARS-CoV-2”“Coronavirus”and“Traditional Chinese Medicine”were searched in var-ious databases like ScienceDirect,Google Scholar,PubMed,and ResearchGate during the time span of *** trials and studies relevant to this study were identified through a systematic search strategy fol-lowing PRISMA ***:In this systematic review,after careful evaluation and reviewing of literature through PRISMA guidelines,145 relevant studies were identified,reviewed and included in this systematic review.A detailed study has been summarized on virus structure,etiology,epidemiology characteristics,pathophysiology,and potential therapeutic treatments and preventive measures adopted in traditional medicinal system in the treatment of infected COVID-19 ***:SARS-CoV-2 transmission dynamics and pathogenesis were revealed by the etiology ***-

关键词： COVID-19 SARS-CoV-2 Structure Epidemiology Pathophysiology Traditional medicine