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技能在发展：全球趋势与地方共鸣——在国际职业技术教育大会开幕式上的致辞

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职业技术教育 2017年第21期38卷 14-15页

作者： stanley.Mutumba Simataa 本刊（整理/译）纳米比亚信息和通信技术部不详

今天我非常高兴，也非常荣幸，能够参加此次国际职业技术教育与培训（TVET）大会。在此，我要向中华人民共和国政府、中华人民共和国UNESCO全国委员会，以及唐山市政府组织召开这次会议表示感谢。

关键词：职业技术教育国际中华人民共和国开幕式致辞共鸣技能 UNESCO

Improved Recovery of Erythropoietin and Darbepoetin from Equine Plasma by the Application of a Wheat Germ Agglutinin Mediated Pre-Extraction Prior to Immunoaffinity Chromatography

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Advances in Bioscience and Biotechnology 2014年第7期5卷 651-660页

作者： Shawn M. R. stanley.Danny Chua The Singapore Turf Club Laboratory 1 Turf Club Ave Singapore Singapore

We describe a two-step method that uses wheat germ agglutinin immobilized on Sepharose gel followed by immunoaffinity chromatography (IAC) to extract recombinant human erythropoietin and Darbepoetin from equine plasma. Lectin affinity chromatography was shown to be an effective approach for isolating these epoetins from plasma and in combination with IAC;this method gave superior recovery when compared to the use of the latter technique alone. Moreover, due to the ease with which it can be scaled up, it is particularly well suited for pre-concentrating larger volumes of samples prior to IAC and this provides a facile way of improving the overall sensitivity with which these foreign proteins can be detected in equine plasma.

关键词： Erythropoietin Darbepoetin Alfa Immunoaffinity Extraction Lectin Affinity Extraction Wheat Germ Agglutinin Horseracing

A Quantum Chemical Screening of Two Imidazole-Chalcone Hybrid Ligands and Their Pd, Pt and Zn Complexes for Charge Transport and Nonlinear Optical (NLO) Properties: A DFT Study

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Computational Chemistry 2021年第4期9卷 215-237页

作者： Fritzgerald Kogge Bine Numbonui stanley.Tasheh Julius Numbonui Ghogomu Research Unit of Noxious Chemistry and Environmental Engineering Department of Chemistry Faculty of Science Univer-sity of Dschang Dschang Cameroon Department of Chemistry Faculty of Science The University of Bamenda Bambili-Bamenda Cameroon

A quantum chemical screening of two imidazole-based chalcone ligands: 2- [1-(3-(1H-imidazol-1-yl)propylimino)-3-(phenylallyl)]phenol and 2-[1-(3-(1H- imidazol-1-yl)propylimino)-3-4-nitrophenylallyl]phenol (hereinafter referred to as HL1 and HL2 respectively) and their Pd, Pt and Zn chelates for charge transport and nonlinear optical (NLO) properties, is reported via dispersion- corrected density functional theory (DFT-D3) and time-dependent DFT (TD- DFT) methods. From our results, Pd and Pt complexes have been observed to show excellent hole-transport properties, owing to their very small reorganization energies. The light extraction efficiency of the HL1-Pt complex was deduced to be particularly impressive, thus suitable for the manufacture of hole transport layer in violet light emitting diodes (LEDs). Moreover, redox potentials and chemical stability studies have enabled us to validate the greater stability in moisture (towards oxidation), of HL2 complexes compared to their HL1 counterparts. The first and second hyperpolarizabilities of both ligands and their complexes have been found to be outstandingly higher than those of the push-pull prototypical, para-nitroaniline by factors of up t

关键词： Density Functional Theory Time-Dependent Density Functional Theory Chalcone-Imidazoles Charge Transport and Nonlinear Optical Properties

Comparison between Hydroponic and Soil-Grown Strawberries: Sensory Attributes and Correlations with Nutrient Content

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Food and Nutrition Sciences 2015年第15期6卷 1371-1380页

作者： Chenin Treftz Zhang stanley.T. Omaye Environmental Sciences and Health Graduate Program Agriculture Nutrition and Veterinary Sciences Department University of Nevada Reno USA

Hydroponic growing methods are growing in popularity and seem to have numerous benefits (i.e., environmental, increased product yields, year round growing) compared to soil grown crops. Although these advantages are attractive, they do not guarantee a high quality product. Taste is a driver of consumer acceptance;therefore, sensory analysis of the hydroponic product will be an important indicator in its success. In this study, we evaluated the sensory differences and preferences in hydroponically grown and soil-grown strawberries (Fragaria x ananassa) using unspecified discriminatory and preference analyses, and descriptive testing correlated with nutrition content data. Most (87%) of participants could identify differences between hydroponically and soil grown strawberries and 70% preferred the hydroponically grown strawberry (p = 0.06). The nutrient composition of the strawberries significantly influenced several sensory analysis categories (sweetness, overall flavor and overall taste (p < 0.05)). The use of sensory studies in relation to consumer acceptance and nutrient quality will be an important factor to consider for exploring growing methods and techniques in hydroponic technology.

关键词： Hydroponic Sensory Evaluation Sustainable Foods Strawberry Greenhouse

Cancer immunotherapy: a brief review of the history, possibilities, and challenges ahead

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Journal of Cancer Metastasis and Treatment 2017年第1期3卷 250-261页

作者： stanley.J.Oiseth Mohamed S.Aziz Department of Pathology American University of the Caribbean School of MedicineCupecoySt.MaartenNetherlands Antilles.

The knowledge that the body possesses natural defenses to combat cancer existed long before the modern period,with multiple anecdotal reports of tumors miraculously disappearing,sometimes spontaneously or after a febrile or infectious *** tumor regression of untreated malignant tumors is currently a well-accepted albeit rare phenomenon,and it is recognized that immunosuppression is associated with a higher cancer *** treatment of bladder carcinoma by intravesical administration of live attenuated Bacillus Calmette-Guérin bacteria was shown to be very effective in 1976 and is now standard *** immunity against cancer involves complex interactions between the tumor,the host,and the *** immunotherapy uses various strategies to augment tumor immunity and represents a paradigm shift in treating cancer,since attention has become more focused on the“biologic passport”of the individual tumor rather than the site of origin of the *** different types of cancer immunotherapies discussed here include biologic modifiers,such as cytokines and vaccines,adoptive cell therapies,oncolytic viruses,and antibodies against immune checkpoint inhibitors,such as the co-inhibitory T-cell receptor PD-1 and one of its ligands,programmed death-ligand 1.

关键词： Cancer immunotherapy immune checkpoint inhibitors PD-1 programmed death-ligand 1 cytotoxic T-lymphocyte-associated antigen-4 adoptive cell therapy cancer vaccines oncolytic viruses history of cancer immunology

Improved Protein Transfer Efficiency and Signal Intensity in BlotMan Using Pulse Width Modulation

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Journal of Biomedical Science and Engineering 2016年第5期9卷 269-279页

作者： Thuc Nhi Truongvo Feixue Zheng stanley.Chien Hiroki Yokota Department of Biomedical Engineering Indiana University Purdue University Indianapolis Indianapolis IN USA YC Bioelectric LLC Indianapolis IN USA Department of Electrical and Computer Engineering Indiana University Purdue University Indianapolis Indianapolis IN USA

BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.

关键词： Pulse Width Modulation (PWM) Western Blotting Protein Transfer

白细胞介素5受体α链在支气管上皮细胞BEAS-2B的表达

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中华微生物学和免疫学杂志 2007年第1期27卷 69-73页

作者：汤葳 Albert Chan stanley.Chik Adrain Wu 邓伟吾上海交通大学医学院附属瑞金医院呼吸科 200025 香港大学附属玛丽医院免疫和变态反应科

目的白细胞介素5（n5）作为参与嗜酸粒细胞成熟、分化过程的最主要因子，其作用通过特异性受体IL-5Rα介导。本文以支气管上皮细胞株为研究对象，证实该上皮细胞株在炎症因子的刺激下可以表达IL-5Rα。方法通过促炎症因子TNF-α和TH2型... 详细信息

目的白细胞介素5（n5）作为参与嗜酸粒细胞成熟、分化过程的最主要因子，其作用通过特异性受体IL-5Rα介导。本文以支气管上皮细胞株为研究对象，证实该上皮细胞株在炎症因子的刺激下可以表达IL-5Rα。方法通过促炎症因子TNF-α和TH2型细胞因子IL-4、IL-5、IL-13以及白三烯成分之一的LTC4（白三烯C4）对细胞进行单独或协同刺激，以RT-PCR方法、流式细胞检测技术评价上皮细胞对IL-5Rα的基因和蛋白质的表达，同时评价在IL-5和TNF-α的协同刺激下IL-5Rα、ICAM-1和VCAM-1的协同表达。结果正常支气管上皮细胞在TH2型细胞因子、LTC4和TNF-α的刺激下可以表达IL-5Rα基因，在TNF-α单独或与其他因子的协同刺激下表达显著增强，更可以表达IL-5Rα蛋白。流式细胞检测分析显示TNF-α单独或协同刺激后16h IL-5Rα蛋白表达最高，并随时间的延长表达下降。当TNF-α与IL-5协同刺激后上皮细胞可以同时表达VCAM-1和IL-5Rα，而ICAM-1呈持续表达。结论通过对支气管上皮细胞的研究证实了支气管上皮细胞可以在炎症因子仆Bα和TH2型细胞因子的协同刺激下进一步表达IL-5特异性受体IL-5Rα，并在IL-5和TNF-α的刺激下进一步表达黏附分子VCAM-1，吸引嗜酸粒细胞及其前体细胞浸润至气道参与炎症。说明支气管上皮细胞不仅在哮喘炎症反应中是最主要的受累器官，也是导致炎症的参与者之一。

关键词： IL-5Rα VCAM-1 支气管上皮细胞哮喘

Randomized trial in malignant biliary obstruction:Plastic vs partially covered metal stents

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World Journal of Gastroenterology 2013年第46期19卷 8638-8646页

作者： Peter L Moses Khalid M AlNaamani Alan N Barkun Stuart R Gordon Roger D Mitty M stanley.Branch Thomas E Kowalski Myriam Martel Viviane Adam Division of Gastroenterology and Hepatology University of Vermont Division of Gastroenterology McGill University Health Center McGill University Department of Epidemiology and Biostatistics and Occupational Health McGill University Health Center Division of Gastroenterology and Hepatology Dartmouth Hitchcock Medical Center Lebanon Division of Gastroenterology Tufts University School of Medicine Boston Division of Gastroenterology Duke University Medical Center Durham Division of Gastroenterology and Hepatology Thomas Jefferson University Hospital

AIM:To compare efficacy and complications of par-tially covered self-expandable metal stent(pcSEMS)to plastic stent(PS)in patients treated for malignant,infrahilar biliary ***:Multicenter prospective randomized clinical trial with treatment allocation to a pcWallstent(SEMS)or a 10 French *** patients aged≥18,for infrahilar malignant biliary obstruction and a Karnofsky performance scale index>60%from 6 participating North American university *** endpoint was time to stent failure,with secondary outcomes of death,adverse events,Karnofsky performance score and short-form-36 scale administered on a three-monthly basis for up to 2 *** analyses were performed for stent failure and death,with Cox proportional hazards regression models to determine significant predictive ***:Eighty-five patients were accrued over 37mo,42 were randomized to the SEMS group and 83patients were available for *** to stent failure was 385.3±52.5 d in the SEMS and 153.3±19.8 d in the PS group,P=*** to death did not differ between groups(192.3±23.4 d for SEMS vs211.5±28.0 d for PS,P=0.70).The only significant predictor was treatment allocation,relating to the time to stent failure(P=0.01).Amongst other measured outcomes,only cholangitis differed,being more common in the PS group(4.9%vs 24.5%,P=0.029).The small number of patients in follow-up limits longitudinal assessments of performance and quality of *** an initially planned 120 patients,only 85 patients were ***:Partially covered SEMS result in a longer duration till stent failure without increased complication rates,yet without accompanying measurable benefits in survival,performance,or quality of life.

关键词： Randomized Biliary Obstruction Stent Plastic Metal Palliative Common bile duct

Laryngeal Stenosis from Trapped Overflowed Head Scarf in the Wheel of a Moving Motor Cycle: A Case Report

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Case Reports in Clinical Medicine 2016年第10期5卷 347-352页

作者： stanley.Baba Amutta Mohammed Abdullahi Daniel Aliyu Caleb Manya Department of Otorhinolaryngology Usmanu Danfodiyo University Teaching Hospital Sokoto Nigeria

Introduction: Laryngeal stenosis is partial or circumferential narrowing of the endolaryngeal airway from congenital or acquired causes. It can present with life threatening upper airway obstruction, and the goal of treatment is restoration of good quality voice, respiration and laryngeal competence. We report a case of laryngeal stenosis following strangulation by a trapped overflowed head scarf, whilst being transported on a motor cycle and highlighted the treatment challenges. Methodology: A case of laryngeal stenosis studied, clinical findings, investigation results and treatment analysed. Results: A 70-year-old Nigerian elderly woman presented with three weeks’ history of hoarseness and progressive difficulty in breathing, following pulling of her long head scarf into the back wheel of the motor cycle taking her home. She had immediate loss of consciousness, which lasted for about 60 minutes. There was associated bout of cough while eating. Examination revealed life threatening upper airway obstruction. X-ray soft tissue neck showed soft tissue mass obliterating larygotracheal air column. Flexible nasopharyngolaryngoscopy showed grossly distorted laryngeal inlet with invisible vocal cords. CT scan of the larynx showed multiple and displaced fractures of the laryngeal cartilages. Microlaryngoscopy findings were pin-hole laryngeal lumen with firm collapsed mucosa. Emergency tracheostomy was done to relieve the upper airway obstruction. Attempts were made to excise the collapse mucosa and insert a stent proved difficult. Referral for endoscopic laser excision was caution because of the potential risk of aspiration. She was counselled and coping well with the tracheostomy.

关键词： Laryngeal Stenosis Strangulation Treatment Sokoto