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A field guide for aging passerine nestlings using growth data and predictive modeling

Avian Research 2021年第2期12卷 141-149页

作者： Audrey A.Sanchez Andrew W.Bartlow allison M.Chan Jeanne M.Fair Aaron A.Skinner Kelly Hutchins Maria A.Musgrave emily M.Phillips Brent e.Thompson Charles D.Hathcock environmental Stewardship Los Alamos National LaboratoryLos AlamosNMUSA Biosecurity and Public Health Los Alamos National LaboratoryLos AlamosNMUSA Water Program N3BLos AlamosNMUSA

Background:Accurate nestling age is valuable for studies on nesting strategies,productivity,and impacts on repro-ductive *** aging guides consist of descriptions and photographs that are time consuming to read and subjective to *** Western Bluebird(Sialia mexicana)is a secondary cavity-nesting passerine that nests in coniferous and open deciduous *** box programs for cavity-nesting species have provided suitable nesting locations and opportunities for data collection on nestling growth and ***:We developed models for predicting the age of Western Bluebird nestlings from morphometric meas-urements using model training and *** were developed for mass,tarsus,and two different culmen ***:Our models were accurate to within less than a day,and each model worked best for a specific age *** mass and tarsus models can be used to estimate the ages of Western Bluebird nestlings 0-10 days old and were accurate to within 0.5 days for mass and 0.7 days for *** culmen models can be used to estimate ages of nest-lings 0-15 days old and were also accurate to within less than a *** daily mean,minimum,and maximum values of each morphometric measurement are provided and can be used in the field for accurate nestling age estimations in real ***:The model training and validation procedures used here demonstrate that this method can create aging models that are highly *** methods can be applied to any passerine species provided sufficient nest-ling morphometric data are available.

关键词： Cavity-nesting Nest boxes Nestling development Predictive models Western Bluebird

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Study of Aerosol Direct and Indirect effects and Auto-conversion Processes over the West African Monsoon Region Using a Regional Climate Model

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Advances in Atmospheric Sciences 2018年第2期35卷 182-194页

作者： Zeinab SALAH Ahmed SHALABY allison L. STeINeR Ashraf S. ZAKeY Ritesh GAUTAM Mohamed M. ABDeL WAHAB The egyptian Meteorological Authority Cairo 11784 Egypt University of Michigan Department of Climate and Space Sciences and Engineering Ann Arbor M148109-2143 USA Centre of Studies in Resources engineering and Interdisciplinary Program in Climate Studies Indian Institute of Technology Bombay Bombay 400076 India Cairo University Department of Astronomy Space Science and Meteorology Giza 12613 Egypt environmental Defense Fund Washington DC 20009 USA

This study assesses the direct and indirect effects of natural and anthropogenic aerosols （e.g., black carbon and sulfate） over West and Central Africa during the West African monsoon （WAM） period （June-July-August）. We investigate the impacts of aerosols on the amount of cloudiness, the influences on the precipitation efficiency of clouds, and the associated radiative forcing （direct and indirect）. Our study includes the implementation of three new formulations of auto-conversion parameterization [namely, the Beheng （BH）, Tripoli and Cotton （TC） and Liu and Daum （R6） schemes] in RegCM4.4.1, besides the default model＇s auto-conversion scheme （Kessler）. Among the new schemes, BH reduces the precipitation wet bias by more than 50% over West Africa and achieves a bias reduction of around 25% over Central Africa. Results from detailed sensitivity experiments suggest a significant path forward in terms of addressing the long-standing issue of the characteristic wet bias in RegCM. In terms of aerosol-induced radiative forcing, the impact of the various schemes is found to vary considerably （ranging from -5 to -25 W m-2）.

关键词： aerosol, cloud West African monsoon auto-conversion RegCM

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大肠杆菌vpr基因突变株的构建及其特性鉴定

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中国兽医学报 2003年第1期23卷 49-51页

作者：严亚贤陆承平 Heather e.allison 上海交通大学农学院动物科学系上海201101 南京农业大学农业部动物疫病诊断与免疫重点开放实验室江苏南京210095 英国利物浦大学环境与分子微生物系

将重组自杀性质粒 p YYvpr转化到含全 vpr基因的 *** MC10 6 1感受态细胞中 ,利用卡那霉素抗性筛选到 6个菌落 ,经 PCR和 Southern blot进一步验证 ,得到一个可靠的 vpr同源基因突变株 ,即 MC10 6 1△ vpr。该突变株与亲本 MC10 6 1具... 详细信息

将重组自杀性质粒 p YYvpr转化到含全 vpr基因的 *** MC10 6 1感受态细胞中 ,利用卡那霉素抗性筛选到 6个菌落 ,经 PCR和 Southern blot进一步验证 ,得到一个可靠的 vpr同源基因突变株 ,即 MC10 6 1△ vpr。该突变株与亲本 MC10 6 1具有相似的生长特征。噬菌体裂解试验表明 ,突变株对 VT2噬菌体 C43b不敏感 ,而 MC10 6 1敏感 ,可见大量的蚀菌斑。噬菌体溶原试验表明 ,MC10 6 1和突变株 MC10 6 1△vpr对噬菌体的敏感性无差别。所有这些表明 ,vpr基因与 VT2噬菌体的裂解性有关 ,可能是编码 VT2噬菌体受体蛋白的基因。

关键词：大肠杆菌 VPR基因突变体构建特性鉴定

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Layer-by-layer functionalized nanotube arrays:A versatile microfluidic platform for biodetection

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Microsystems & Nanoengineering 2015年第1期1卷 1-7页

作者： allison L.Yost Setareh Shahsavari Grinia M.Bradwell Roberta Polak Fabio Fachin Robert e.Cohen Gareth H.McKinley Mehmet Toner Michael F.Rubner Brian L.Wardle Department of Mechanical engineering Massachusetts Institute of Technology77 Massachusetts AvenueCambridgeMA 02139USA Department of Materials Science and engineering Massachusetts Institute of Technology77 Massachusetts AvenueCambridgeMA 02139USA Department of Chemical engineering Massachusetts Institute of Technology77 Massachusetts AvenueCambridgeMA 02139USA Department of Aeronautics and Astronautics Massachusetts Institute of Technology77 Massachusetts AvenueCambridgeMA 02139USA Center for engineering in Medicine and BioMeMS Resource Center Massachusetts General HospitalHarvard Medical Schooland Shriners Hospital for ChildrenBostonMA 02139USA

We demonstrate the layer-by-layer(LbL)assembly of polyelectrolyte multilayers(PeM)on three-dimensional nanofiber *** porosity(99%)aligned carbon nanotube(CNT)arrays are photolithographically patterned into elements that act as textured scaffolds for the creation of functionally coated(nano)porous ***-scale bilayers of poly(allylamine hydrochloride)/poly(styrene sulfonate)(PAH/SPS)are formed conformally on the individual nanotubes by repeated deposition from aqueous solution in microfluidic *** and experimental results show that the LbL deposition is dominated by the diffusive transport of the polymeric constituents,and we use this understanding to demonstrate spatial tailoring on the patterned nanoporous elements.A proof-of-principle application,microfluidic bioparticle capture using N-hydroxysuccinimide-biotin binding for the isolation of prostate-specific antigen(PSA),is demonstrated.

关键词： layer-by-layer carbon nanotube microfluidic nanoporous functionalization bioparticle capture

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London Poems and History

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Journal of Literature and Art Studies 2016年第5期6卷 462-467页

作者： Tzu Yu allison Lin Gaziantep University Gaziantep Turkey

In this article, I read different poems of London through the perspectives of time and the self. The city of London, as a physical space, a world in the Alfred Tennyson＇s Cleopatra＇s Nee history to Tennyson＇s Victorian Lo globe, is changing dle, the flow of tid ndon. The Needle through both inner time and outer time. Firstly, in Lord e symbolises the passing time, through the long egyptian has been through different seas and places. The sense of history, a fusion of inner time and outer time, is claimed by the Needle＇s subjective self, seeing London as a ＂monster town＂. Secondly, Ahren Wamer＇s Greek titled poem is trying to locate the one in London, which cannot be localized, in the trend of globalization, as the gazer observed on the bus. Struggling between the self and the other, inner and outer existences, happiness and being unhappy, W. B. Yeats＇ from Vacillation comes to show the reader that through reflection and memory, the sense of one＇s own self can be reinforced and affirmed, while creating one＇s own personal history. Last but not the least, I read a part from T. S. eliot＇s Four Quartets As the dialectic of light and shadow plays a sense of Beauty, the soul is aware of all fancy things, but only without any meanings. The question of the self and tradition, the poet and the world, somehow, is a timeless one

关键词： London self time history tradition

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Fundamental molecular biology.影印版

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丛书名：国外优秀生命科学教学用书 (影印版)

2008年

作者： Lizabeth A. allison

"教育部高等教育司推荐."

ISBN: (纸本)9787040250572

"教育部高等教育司推荐."

关键词： Molecular biology

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大肠杆菌VT噬菌体受体(vpr)基因突变株的鉴定

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微生物学报 2002年第6期42卷 745-747页

作者：严亚贤孙建和陆承平 Heather e allison 上海交通大学农业与生物学院上海201101 南京农业大学农业部动物疫病诊断与免疫重点开放实验室南京210095 英国利物浦大学生物科学院环境与分子微生物系

The recombinant suicide plasmid pYYvpr contained a Kan resistant cassette and a 778bp internal piece of the vpr gene. It was transformed into the wild type *** strain, MC 1061,which possessed the full-length vpr gene and did not contain the λ pir gene. The recombinant suicide plasmid could not replicate in MC 1061. Colonies grew on the plates of Kan were analysed by PCR and Southern blot with two different Dig-probes,vpr and Kan R probes. One colony was detected vpr and Kan R gene by PCR and Southern blot showed the mutant had the different vpr map with the wild type. The isogenic knockout mutant, named MC1061 Δvpr,was *** mutant supported lysogenic infection of VT2 recombinant phage, but did not support lytic infection. All results showed that the vpr gene should be related to the lytic infection of VT2 phage.

关键词： VT噬菌体受体 vpr基因突变鉴定大肠杆菌0157 强致病性

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Cold adaptation does not alter ATP homeostasis during cold exposure in Drosophila melanogaster

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Integrative Zoology 2018年第4期13卷 471-481页

作者： Caroline M.WILLIAMS James R.ROCCA Arthur S.eDISON David B.allison Theodore J.MORGAN Daniel A.HAHN Department of Integrative Biology University of CaliforniaBerkeleyCaliforniaUSA Departments of entomology and Nematology University of FloridaGainesvilleFloridaUSA Biochemistry University of FloridaGainesvilleFloridaUSA Advanced Magnetic Resonance Imaging and Spectroscopy Facility McKnight Brain InstituteUniversity of FloridaGainesvilleFloridaUSA Departments of Genetics and Biochemistry Complex Carbohydrate Research CenterUniversity of GeorgiaAthensGeorgiaUSA Department of epidemiology and Biostatistics School of Public HealthIndiana University BloomingtonBloomingtonIndianaUSA Division of Biology Kansas State UniversityManhattanKansasUSA

In insects and other ectotherms,cold temperatures cause a coma resulting from loss of neuromuscular function,during which ionic and metabolic homeostasis are progressively *** adaptation improves homeostasis during cold exposure,but the ultimate targets of selection are still an open *** acclimation and adaptation remodels mitochondrial metabolism in insects,suggesting that aerobic energy production during cold exposure could be a target of ***,we test the hypothesis that cold adaptation improves the ability to maintain rates of aerobic energy production during cold exposure by using^(31)P NMR on live *** lines of Drosophila melanogaster artificially selected for fast and slow recovery from a cold coma,we show that cold exposure does not lower ATP levels and that cold adaptation does not alter aerobic ATP production during cold ***-hardy and cold-susceptible lines both experienced a brief transition to anaerobic metabolism during cooling,but this was rapidly reversed during cold exposure,suggesting that oxidative phosphorylation was sufficient to meet energy demands below the critical thermal minimum,even in cold-susceptible *** thus reject the hypothesis that performance under mild low temperatures is set by aerobic ATP supply limitations in ***,excluding oxygen and capacity limitation as a weak link in energy *** work suggests that the modulations to mitochondrial metabolism resulting from cold acclimation or adaptation may arise from selection on a biosynthetic product(s)of those pathways rather than selection on ATP supply during cold exposure.

关键词： chill coma energetics insect oxygen limitation thermal tolerance

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Understanding of the influence of process parameters on the heat transfer behavior at the metal/die interface in high pressure die casting process

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中国科学:技术科学 2010年第4期 455-455页

作者： allison John Research & Advanced engineering Department Scientific Research LaboratoryFord Motor Company

The current paper focuses on the influence of the process parameters on the peak values of the interfacial heat transfer coefficient(IHTC)at metal/die interface during high pressure die casting(HPDC)process.A"step shape"casting and AM50 alloy were used during the *** IHTC was determined by solving the inverse thermal problem based on the measured temperature inside the *** show that the initial die surface temperature(IDST,TDI)has a dominant influence while the casting pressure and fast shot velocity have a secondary influence on the IHTC peak *** curve fitting,it was found that the IHTC peak value(hmax)changes as a function of the IDST in a manner of hmax=e αTγ DI .Such relationship between the IHTC peak value and the IDST can also be found when the casting alloy is ADC12,indicating that this phenomenon is a common characteristic in the HPDC process.

关键词： high pressure die casting interfacial heat transfer coefficient AM50 ADC12

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Uncovering the immunological repertoire of the carpet shell clam Ruditapes decussatus through a transcriptomic-based approach

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Aquaculture and Fisheries 2019年第1期4卷 37-42页

作者： Frederico M.Batista allison M.Churcher Manuel Manchado Alexandra Leitao Deborah M.Power Centro de Cieencias do Mar(CCMAR) Universidade do AlgarveCampus de Gambelas8005-139 FaroPortugal Instituto Portugues do Mar e da Atmosfera Estaçao Experimental de Moluscicultura de TaviraPortugal Department of Biosciences College of ScienceSwansea UniversitySwanseaSA28PPWalesUnited Kingdom IFAPA Centro el Toruno Junta de AndalucíaCamino Tiro Pichon sn11500 El Puerto de Santa MaríaCadizSpain environmental Science Center(eSC) Qatar UniversityP.O.Box 2713 DohaQatar

The grooved carpet-shell clam Ruditapes decussatus is native to the Northern Atlantic and Mediterranean Sea and has a high commercial *** is one of the main native bivalve species cultured in *** main objective of the present study was to gain further insights into the immunological repertoire of *** through a transcriptomic *** mantle samples of eight *** individuals were sequenced using Illumina platform.A total of 67132 contigs with more than 800 bp were *** annotation of these contigs revealed 146 immune-related *** gene families in which the highest number of immune-related genes was observed were:C1q domain-containing proteins(63),tumor necrosis factors(15)and toll-like receptors(TLRs,10).A total of 5359 putative single nucleotide polymorphisms(SNPs)were identified in the 146 immune-related *** density of SNPs ranged between 0.04 and 7.92 SNPs/100 *** highest and the lowest SNP density were observed in genes of the C1q domain-containing protein *** to the importance of TLRs in innate immunity,we focused our attention on these membrane *** TLRs were identified based on protein domain *** analysis revealed that *** TLRs were diverse and only 3 showed orthology with TLRs of known immune functions in other bivalve ***,our analysis suggests that lineage restricted-expansions of TLRs occurred in all mollusc taxa analysed including in venerids.

关键词： Ruditapes decussatus Transcriptome Immune genes Toll-like receptors

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