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Asian Journal of Andrology 2004年第4期6卷 359-364页

作者： Gustavo F.Gonzales Stella Hartinger Yetssy flores Kelly Zárate Pedro Yi Instituto de Investigations de la Altura Department of Biological and Physiological SciencesDepartment of Biological and Physiological SciencesDepartment of Biological and Physiological SciencesFaculty of Veterinary Medicine and Animal Sciences Department of Biological and Physiological Sciences and and and and Universidad Peruana Cayetano Heredia. Postal Office 1843. Lima Peru

Aim: To evaluate the effect of a protein synthesis inhibitor cycloheximide on arresting activity in spermato-genesis and sperm count in male rats. Methods: The study used seminiferous tubule (ST) segments from adult rats cultured in vitro with or without cycloheximide to condition culture media, which have been concentrated, size fractioned (30-50 kDa) and administered 7 days to adult rats by intraperitoneal injections. The effects on testicular and epididymal weights, spermatogenesis and epididymal sperm count were determined. Results: The fraction (30-50 kDa), named arresting, obtained from the culture without cycloheximide decreased testicular and epididymal weights (P<0.01) and reduced the epididymal sperm count significantly. Study of the spermatogenic cycle by transillumination showed spermatogenic arrest at stage VII in rats treated with arresting compared to that observed in controls. The length of stage VII in the group receiving the seminiferous tubules culture media with cycloheximide (30-50 KDa CHX-STCM fraction) was similar to control. Conclusion: The difference in the effect may be the result of the presence or absence of arresting, a protein secreted by the tubules.

关键词： cycloheximide arresting conditioned medium seminiferous tubule sperm production transillumination

KCl:Eu^(2+) as a solar UV-C radiation dosimeter. Optically stimulated luminescence and thermoluminescence analyses

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Journal of Rare Earths 2009年第4期27卷 579-583页

作者： I.Aguirre de Cárcer H.L.D'Antoni M.Barboza-flores V.Correcher F.Jaque Departamento Física de Materiales Universidad Autónoma Madrid Spain NASA Ames Research Center Moffett Field California USA Centro de Investig ción en Física Universidad de Sonora México Centro Investigaciones Energía y Medioambientales (CIEMAT) Madrid Spain

The KCI：Eu2＋ system response to UV-C was investigated by analyzing the optically stimulated luminescence （OSL） and ther- mo-luminescence （TL） signal produced by ultraviolet light exposure at room temperature. It was found that after UV-C ***, OSL was produced on a wide band of visible wavelengths with decay time that varied by several orders of magnitude depending on the Eu2＋ aggregation state. In spite of the low intensity of solar UV-C reaching the Earth＇s surface in Madrid （40° N, 700 m a.s.1.）, it was possible to measure the UV-C radiation dose at 6：48 solar time by using the TL response of the KCI：Eu2＋ system and differentiate it from the ambient beta radiation dose.

关键词： thermo-luminescence rare-earth-doped alkali halides solar ultraviolet dosimeter environmental radiation

Convergence of the turkey gut microbiota following cohabitation under commercial settings

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Journal of Animal Science and Biotechnology 2021年第4期12卷 1635-1644页

作者： Elizabeth A.Miller Brittanie Winfield Bonnie P.Weber Cristian flores-Figueroa Jeannette Munoz-Aguayo Jared Huisinga Timothy J.Johnson Department of Veterinary and Biomedical Sciences University of MinnesotaSaint PaulMNUSA University of Minnesota Mid-Central Research and Outreach CenterWillmarMNUSA Science Innovations WillmarMNUSA

Background:Microbiota development is a critical aspect of turkey poult maturation,and the succession of microbes in the turkey gut has been shown to correlate with poult *** purpose of this study was to determine the fate of the microbiota in turkey poults after movement of birds first raised in an isolated hatch brood system into a more traditional commercial brood facility with pre-existing *** poults were first divided into groups raised in conventional brood pens from day-of-hatch and those raised in an experimental hatch brood *** 11 days of growth,hatch brood birds were moved into pens within the conventional brood barn and monitored for an additional 18 *** of both hatch brood and conventional pen birds was performed at multiple timepoints throughout the study,and cecal content was used to analyze the bacterial microbiota using 16S rRNA gene amplicon ***:Alpha diversity tended to be higher in samples from conventional pen birds compared to those from hatch brood birds prior to the day 11 move,but the difference between systems was not observed *** beta diversity metrics,bacterial community succession appeared delayed in the hatch brood system birds pre-move,but post-move community composition quickly converged with that of the conventional pen *** was validated through assessment of significantly different genera between hatch brood system and conventional pen birds,where numbers of significantly different taxa quickly decreased following the *** key taxa previously associated with poult performance were delayed in their appearance and relative abundance in hatch brood ***:Overall,this study demonstrates that the use of isolated hatch brood systems has an impact on the poult gut microbiota,but its impact is resolved quickly once the birds are introduced into a conventional brood ***,the benefits of pathogen reduction with hatch brood systems may out

关键词： Brood Gut Hatch Microbiota Poult Succession Turkey

Microbial and size characterization of airborne particulate matter collected on sticky tapes along US–Mexico border

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Journal of Environmental Sciences 2017年第3期29卷 207-216页

作者： Amir Gonzalez-Delgado Manoj K.Shukla David W.DuBois Juan P.flores-Mdrgez Joel A.Hernandez Escamilla Evangelina Olivas Plant and Environmental Sciences Department New Mexico State UniversityMSC 3Q Skeen Hall Room N 127P.O.Box 30003Las CrucesNM 88003-8003USA Universidad Autonoma de Ciudad Judrez UACJAv.Plutarco Elias Calles 1020Fovisste ChamizalCiudad JudrezChihuahua C.P.32310Mexico

Particulate matter （PM） emissions from various sources can affect significantly human health and environmental quality especially in the Chihuahuan Desert region along US- Mexico border. The objective of this study was to use the low-cost sticky tape method to collect airborne PM for size characterization and identification of fungal spores. Sticky tape samplers were placed at 1.0 and 2.0 m above the ground surface at experimental sites in Ciudad Ju＆ez, Mexico and at 0.6, 1.2 and 1.8 m at New Mexico sites, USA. Soil samples were collected in both countries to determine fungal diversity, texture and moisture content. Dust particles collected from all of the experimental sites had a dominant texture of clay （〈0.002 mm）. The dominant textures identified from soil samples collected from the US and Mexican sites were loam and sandy clay loam, respectively. Alternaria, Penicillium and Fusarium were frequently found funguses in the US sites while Alternaria and Aspergillus were commonly observed in the Mexican sites. The sticky tapes also showed a similar diversity of fungal microorganisms present in the airborne PM at both Mexico and US sites. Alternaria, Penicillium and Aspergillus were the three groups of airborne fungal microorgan- isms consistently present in the US and Mexican sites. The low-cost sticky tape method has the potential to be used for characterizing different airbome microorganisms and dust particles.

关键词： Fungal sporesSticky tapeParticle sizeMicroorganismDust

Microwave-ultrasound assisted extraction of red corn pigments and their effect on chemical composition and tecno-functional properties

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Food Bioscience 2022年第6期50卷 1111-1119页

作者： J.D.Garcia-Ortiz A.C.flores-Gallegos J.A.Ascacio-Valdés C.M.López-Badillo S.D.Nery-flores S.C.Esparza-González R.Rodríguez-Herrera School of Chemistry Autonomous University of CoahuilaCampus SaltilloSaltilloCoahuila25280Mexico School of Dentistry Autonomous University of CoahuilaCampus SaltilloSaltilloCoahuila25125Mexico

Currently, there is a growing interest in obtaining natural pigments, without presenting a risk to health, as well as to the matrix and food safety. The objective of this study was to characterize grains with different shades of red color (High pigmentation maize = RA, Medium pigmentation maize = RM, and Low pigmentation maize = RB, reddish maize with white segments = VO and white maize control = SC). The physical properties (kernel weight, length, width, thickness, and grain and flour color), chemical composition (moisture, fat, protein, fiber, starch, ash, and minerals), and techno-functional properties (capacity of absorption of organic molecules (OMAC), swelling (CH), oil retention (CAAC), and water absorption (WAS)) variables were measured. Afterward, RM was selected, and a pigment extraction (EAUM) was carried out using microwave-ultrasound equipment with different ratios of ethanol (0, 30, and 70%) and citric acid ratios (0 and 1%). The color and properties of the solid (starch, protein, fat, and their techno-functional properties) were determined in the aqueous phase and were compared with the properties prior to EAUM. The results indicated that the red grains have higher starch content (57.6%) and reddish colors, in addition, VO presented higher moisture (10.4%), fat (6.7%) and fiber (10.0%). The colors of the pigments obtained with EtOH (30 and 70%) show shades red and yellow. Finally, the EAUM decreased the fat content up to 57%, increased WAS and CAAC in the residual flour and generated changes in OMAC and CH, increasing in aqueous medium and reducing with EtOH.

关键词： Natural colorant Zea mays Green extraction Proximal analysis NIR Agronomic properties

Gamma-aminobutyric acid A receptors in Alzheimer＇s disease： highly localized remodeling of a complex and diverse signaling pathway

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Neural Regeneration Research 2018年第8期13卷 1362-1363页

作者： Andrea Kwakowsky Beatriz Calvo-flores Guzman Karan Govindpani Henry J. Waldvogel Richard L. Faull Centre for Brain Research Department of Anatomy and Medical ImagingFaculty of Medical and Health Sciences University of Auckland AucklandNew Zealand

Alzheimer＇s disease （AD）, the predominant form of dementia, is a chronic, incurable neurodegenerative disorder presenting with symptoms includ- ing progressive memory loss and disturbed emotional state. It has been estimated that dementia affects over 47 million people worldwide （Prince et al., 2015）, and with 60-80% of cases attributable to AD.

关键词： AD highly localized remodeling of a complex and diverse signaling pathway Gamma-aminobutyric acid A receptors in Alzheimer’s disease

The Potential of Cerebrolysin in the Treatment of Schizophrenia

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Pharmacology & Pharmacy 2014年第7期5卷 691-704页

作者： Gonzalo flores Marco Atzori Facultad de Ciencias Universidad Autónoma de San Luis Potosí San Luis Potosí Mexico Laboratorio de Neuropsiquiatría Instituto de Fisiología Universidad Autónoma de Puebla Puebla México

Schizophrenia and psychosis are psychiatric condition whose neural mechanisms are yet incompletely known, and for which pharmacological treatment is too often ineffective in a growing clinical cohort. Recently, dendritic morphological changes in arborization and dendritic spine density in limbic regions has been reported in postmortem tissue from schizophrenic patients and in animal models of schizophrenia, suggesting that the use of medication improving synaptogenesis may be beneficial as additional treatment of psychotic patients. Cerebrolysin (Cbl) is a drug available for clinical with active neuropeptides fragments that mimics the action of endogenous neurotrophic factors such as BDNF, GDNF, CNTF and NGF, which improves the integrity of the neuronal circuits as well as cognitive and behavioral performance by exerting a neuroprotective effect and promoting the generation of new functional synapses. Recent work from our laboratory has shown that Cbl ameliorates synaptic and dendritic pathology in animal models of schizophrenia by increasing synaptic density and restoring neuronal cytoarchitecture. This neuroprotective effect improves the integrity of the neuronal circuits and improves cognitive and behavioral performance. Importantly, Cbl treatment seems to be safe when used in combination with neuroleptics such as risperidone. The present article analyzes the potential of Cbl in the treatment of neurodevelopmental disease, and reviews the current literature on the effects of Cbl in in vivo animal models of neurodevelopmental disorders like schizophrenia.

关键词： Cerebrolysin Neurotrophic Factors Schizophrenia Prefrontal Cortex Hippocampus Amygdala

Thermodynamic and experimental studies on removal of calcium and sulfate ions from recycling water of complex sulfide flotation operations

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Transactions of Nonferrous Metals Society of China 2021年第10期31卷 3116-3127页

作者： Elvis BUSTOS-flores Martha Araceli ELIZONDO-ÁLVAREZ Alejandro URIBE-SALAS Department of Metallurgical Engineering CINVESTAV-IPNUnidad Saltillo25900Ramos ArizpeCoahuilaMexico

A chemical method for removing calcium sulfate saturated solutions(0.016 mol/L CaSO_(4))using barium chloride(BaCl_(2)∙2H_(2)O)and sodium phosphate(Na_(3)PO_(4))was experimentally *** main interest is to remove these ions from the solution through the precipitation of two solid species:sulfate(SO_(4)^(2−))as barite(BaSO4),and calcium(Ca^(2+))as hydroxyapatite(Ca_(5)(PO_(4))_(3)OH).Additionally,a solid/liquid separation method(i.e.,flotation)was explored,using oleic acid and dodecylamine as *** results show that,the chemical treatment of saturated solutions at 60℃,pH 11.5 and using 3.9 g/L BaCl_(2)·2H_(2)O and 1.6 g/L Na_(3)PO_(4),promotes the precipitation of barium sulfate and calcium-deficient hydroxyapatite(Ca_(10−x)(HPO_(4))_(x)(PO_(4))_(6−x)(OH)_(2−x)),with residual concentrations of calcium and sulfate below 0.10 and 5 mg/L,*** residual calcium concentration increases to 28 mg/L when using the same amount of reactants,at temperature and pH values below those *** highest flotation recovery of hydroxyapatite with oleic acid at pH 9.5 was about 80%,while that of barite floated with dodecylamine at pH 6.5 was about 90%.

关键词： calcium sulfate dodecylamine oleic acid barite hydroxyapatite flotation separation

基于无人机图像以及不同机器学习和深度学习模型的小麦倒伏率检测

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智慧农业（中英文） 2021年第2期3卷 23-34页

作者： Paulo flores 张昭北达科他州州立大学农业与生物系统工程系美国北达科他州法戈市58102

小麦在生长过程中发生倒伏会严重影响其产量,因此实时且准确地对小麦倒伏状况监测有很重要的意义。传统的方法采用手工方式生成数据集,不仅效率低、易出错,而且生成的数据集不准确。针对这一问题,本研究提出了一种基于图像处理的自动数... 详细信息

小麦在生长过程中发生倒伏会严重影响其产量,因此实时且准确地对小麦倒伏状况监测有很重要的意义。传统的方法采用手工方式生成数据集,不仅效率低、易出错,而且生成的数据集不准确。针对这一问题,本研究提出了一种基于图像处理的自动数据集生成方法。首先利用无人机在15、46和91 m三个高度采集图像数据;采集完数据后,根据无倒伏、倒伏面积50%的标准对每一块地的小麦倒伏情况进行人工评估;采用三种机器学习(支持向量机、随机森林、K近邻)和三种深度学习(ResNet101、GoogLeNet、VGG16)算法对小麦倒伏检测情况进行分类。结果显示,ResNet101的分类结果优于随机森林,并且在91 m高度采集的数据分类精度并不低于在15 m高度采集的数据。本研究证明了针对在91 m高度采集的无人机图像,采用ResNet101对小麦倒伏率检测是一种有效的替代人工检测的方法,其检测精度达到了75%。

关键词：小麦倒伏率机器学习深度学习数据采集高度无人机 ResNet101

The role of shape and size in the ecology of phytoplankton

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生态科学 2008年第5期27卷 295-299页

作者： Luigi Naselli-flores Department of Botanical Sciences University of Palermo

Phytoplankton is a polyphyletic group of photosynthetic, oxygen producing protoctists and bacteria adapted to persist in suspension and liable to passive movement by wind and current (Reynolds, 2006)[1]. They inhabit ... 详细信息

关键词：浮游植物生态系统自然环境物种