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Tecia solanivora infestation increases tuber starch accumulation in Pastusa Suprema potatoes

Journal of Integrative Plant Biology 2018年第11期60卷 1083-1096页

作者： Pavan Kumar Etzel Garrido Kun Zhao Yi Zheng Saleh Alseekh Erandi Vargas-Ortiz alisdair r.fernie Zhangjun Fei Katja Poveda Georg Jander Boyce Thompson Institute for Plant research 533 Tower RoadIthacaNY 14853USA Department of Entomology Cornell UniversityIthacaNY 14853USA Max Planck Institute of Molecular Plant Physiology Wissenschaftspark GolmAm Muhlenberg 114476 PotsdamGermany department of entomology and nematology university of floridaGainesvilleFL 32611USA unidad de microbiologia basica y aplicada facultad de ciencias naturalesuniversidad autonoma de queretaroAv.de las Ciencias SINJuriquillaquerdtaroMdxico department of molecular biology institute for scientific and technological research of san luis potosiMexico

In response to infestation with larvae of the Guatemalan tuber moth （Tecia solanivora）, some Solanum tuberosum （potato） varieties exhibit an overcompensa- tion response, whereby the total dry mass of uninfested tubers is increased. Here, we describe early responses, within the first few days, of T. solanivora feeding, in the Colombian potato variety Pastusa Suprema. Non- targeted metabolite profiling showed significant second- ary metabolism changes in T. solanivora-infested tubers, but not in uninfested systemic tubers. In contrast, changes in primary metabolism were greater in unin- fested systemic tubers than in the infested tubers, with a notable 80% decline in systemic tuber sucrose levels within 1 d of T. solanivora infestation. This suggested either decreased sucrose transport from the leaves or increased sink strength, i.e., more rapid sucrose to starch conversion in the tubers. Increased sucrose synthesis was indicated by higher rubisco activase and lower starch synthase gene expression in the leaves of infested plants. Elevated sink strength was demonstrated by 45% more total starch deposition in systemic tubers of T. solanivord- infested plants compared to uninfested control plants. Thus, rather than investing in increased defense of uninfested tubers, Pastusa Suprema promotes deposition of photoassimilates in the form of starch as a response to T. solanivora infestation.

关键词： Tecia solanivora infestation increases tuber starch accumulation in Pastusa Suprema potatoes Figure

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A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species

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Plant Communications 2020年第5期1卷 129-140页

作者： Youjun Zhang Moxian Chen Beata Siemiatkowska Mitchell rey Toleco Yue Jing Vivien Strotmann Jianghua Zhang Yvonne Stahl alisdair r.fernie Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Max-Planck-Institut fur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany Department of Biology Hong Kong Baptist Universityand State Key Laboratory of AgrobiotechnologyThe Chinese University of Hong KongShatinHong Kong Institute for Developmental Genetics Heinrich Heine UniversityDusseldorfGermany

Although the use of stable transformation technology has led to great insight into gene function,its application in high-throughput studies remains ***-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis,but this technique does not work efficiently in other plant species,including Arabidopsis *** an efficient high-throughput transient expression system is currently lacking in the model plant species ***,we developed a method that is characterized by high efficiency,reproducibility,and suitability for transient expression of a variety of functional proteins in *** and 7 other plant species,including Brassica oleracea,Capsella rubella,Thellungiella salsuginea,Thellungiella halophila,Solanum tuberosum,Capsicum annuum,and *** of this method was independently verified in three independent research facilities,pointing to the robustness of this ***,in addition to demonstrating the utility of this technique in a range of species,we also present a case study employing this method to assess protein–protein interactions in the sucrose biosynthesis pathway in Arabidopsis.

关键词： transient expression agro-infiltration subcellular localization protein-protein interaction

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Metabolons,enzyme-enzyme assemblies that mediate substrate channeling,and their roles in plant metabolism

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Plant Communications 2021年第1期2卷 13-28页

作者： Youjun Zhang alisdair r.fernie Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 114476 Potsdam-GolmGermany

Metabolons are transientmulti-protein complexes of sequential enzymes that mediate substrate *** differ from multi-enzyme complexes in that they are dynamic,rather than permanent,and as such have considerably lower dissociation *** the fact that a huge number of metabolons have been suggested to exist in plants,most of these claims are erroneous as only a handful of these have been proven to *** believe that physical protein-protein interactions between consecutive enzymes of a pathway should rather be called enzyme-enzyme *** this review,we describe how metabolons are generally assembled by transient interactions and held together by both structural elements and non-covalent *** evidence for their existence comes fromprotein-protein interaction studies,which indicate that the enzymes physically interact,and direct substrate channelingmeasurements,which indicate that they functionally ***,advances in cell biology and proteomics have far outstripped those in classical enzymology and flux measurements,rendering most reports reliant purely on interactome *** developments in co-fractionation mass spectrometry will likely further exacerbate this *** this,only dynamic enzyme-enzyme assemblies in which both physical and functional interactions have been demonstrated should be termed *** discuss the level of evidence for the manifold plant pathways that have been postulated to contain metabolons and then list examples in both primary and secondary metabolism for which strong evidence has been provided to support these *** doing so,we pay particular attention to experimental and mathematical approaches to study metabolons as well as complexities that arise in attempting to follow ***,we discuss perspectives for improving our understanding of these fascinating but enigmatic interactions.

关键词： protein-protein interaction metabolon substrate channeling

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OsGF14b modulates defense signaling pathways in rice panicle blast response

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The Crop Journal 2021年第4期9卷 725-738页

作者： Shijuan Yan Qing Liu Thomas Naake Wenjie Huang Mengyu Chen Qian Kong Sheng Zhang Wenyan Li Xuan Li Qinjian Liu Jianyuan Yang alisdair r.fernie Bin Liu Guangdong Key Laboratory for Crop Germplasm resources Preservation and Utilization Agro-biological Gene Research Center Guangdong Academy of Agricultural Sciences Guangdong Key Laboratory of New Technology in rice Breeding Rice Research Institute Guangdong Academy of Agricultural Sciences Max Planck Institute of Molecular Plant Physiology 4. Proteomics and Metabolomics Facility Institute of Biotechnology Cornell University Guangdong Key Laboratory of New Technology in Plant Protection Plant Protection Research Institute Guangdong Academy of Agricultural Sciences

rice with panicle-blast resistance is needed for stable rice production. Although we have previously demonstrated that OsGF14 b underlies a quantitative trait locus that positively regulates rice panicle blast resistance, the mechanism is still unknown. In this study, a multi-omics approach was used to investigate the possible downstream signaling pathway regulated by Os GF14 b. OsGF14 b both strongly activated the gibberellin biosynthetic pathway during pathogen infection and reprogrammed the lignin biosynthetic pathway. reduced lignin accumulation was observed in glumes of OsGF14 b-overexpressing plants in comparison with the wild type after pathogen inoculation. OsGF14 b activated the auxin and jasmonic acid signaling pathways, but inactivated the salicylic acid signaling pathway. Auxin and jasmonic acid appeared to act independently on OsGF14 b-mediated panicle blast resistance. The roles of gibberellin, lignin, and auxin were different from their roles in leaf blast, suggesting that different mechanisms underlie leaf and panicle blast resistance in rice. This study provides a comprehensive catalog of molecular changes that could be targets for future studies of rice panicle blast resistance.

关键词： rice Panicle blast resistance OsGF14b Gibberellin Phytohormone

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MicroTom Metabolic Network: rewiring Tomato Metabolic regulatory Network throughout the Growth Cycle

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Molecular Plant 2020年第8期13卷 1203-1218页

作者： Yan Li Yang Chen Lu Zhou Shengjie You Heng Deng Ya Chen Saleh Alseekh Yong Yuan rao Fu Zixin Zhang Dan Su alisdair r.fernie Mondher Bouzayen Tao Ma Mingchun Liu Yang Zhang Key Laboratory of Bio-resource and Eco-environment of Ministry of Education College of Life SciencesSichuan UniversityChengdu 610065SichuanPeople's Republic of China Max Planck Institute of Molecular Plant Physiology Am Mühlenberg 114476 Potsdam-GolmGermany Center of Plant Systems Biology and Plant Biotechnology 4000 PlovdivBulgaria GBF University of ToulouseINRACastanet-TolosanFrance

Tomato(Solanum lycopersicum)is a major horticultural crop worldwide and has emerged as a preeminent model for metabolic *** many research efforts have focused on the analysis of metabolite differences between varieties and species,the dynamics of metabolic changes during the tomato growth cycle and the regulatory networks that underlie these changes are poorly *** this study,we integrated high-resolution spatio-temporal metabolome and transcriptome data to systematically explore the metabolic landscape across 20 major tomato tissues and growth *** the resulting MicroTom Metabolic Network,the 540 detected metabolites and their co-expressed genes could be divided into 10 distinct clusters based on their biological *** this dataset,we constructed a global map of the major metabolic changes that occur throughout the tomato growth cycle and dissected the underlying regulatory *** addition to verifying previously well-established regulatory networks for important metabolites,we identified novel transcription factors that regulate the biosynthesis of important secondary metabolites such as steroidal glycoalkaloids and *** findings provide insights into spatiotemporal changes in tomato metabolism and generate a valuable resource for the study of metabolic regulatory processes in model plants.

关键词： tomato metabolome transcriptome transcription factor co-expression flavonoids

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Passing the Baton: Substrate Channelling in respiratory Metabolism

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research 2018年第1期2018卷 128-143页

作者： alisdair r.fernie Youjun Zhang Lee J.Sweetlove Max-Planck-Institute of Molecular Plant Physiology Am Muhlenberg 114476 Potsdam-GolmGermany Center of Plant System Biology and Biotechnology 4000 PlovdivBulgaria Department of Plant Sciences University of OxfordSouth Parks RoadOxford OX13RBUK

Despite species-specifc diferences in the pathways of respiratory metabolism are remarkably conserved across the kingdoms of life with glycolysis,the tricarboxylic acid cycle,and mitochondrial electron transport chain representing the major components of the process in the vast majority of *** addition to being of critical importance in fueling life itself these pathways serve as interesting case studies for substrate channelling with research on this theme having been carried out for over 40 *** we provide a cross-kingdom review of the ample evidence for protein-protein interaction and enzyme assemblies within the three component pathways as well as describing the scarcer available evidence for substrate channelling itself.

关键词： life representing metabolism

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Exploiting Natural Variation in Tomato to Define Pathway Structure and Metabolic regulation of Fruit Polyphenolics in the Lycopersicum Complex

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Molecular Plant 2020年第7期13卷 1027-1046页

作者： Takayuki Tohge Federico Scossa regina Wendenburg Pierre Frasse llse Balbo Mutsumi Watanabe Saleh Alseekh Sagar Sudam Jadhav Jay C.Delfin Marc Lohse Patrick Giavalisco Bjoern Usadel Youjun Zhang Jie Luo Mondher Bouzayen alisdair r.fernie Max-Planck-lnstitut fiir Molekulare Pflanzenphysiologie Am Muehlenberg 114476 Potsdam-Golm.Germany Graduate School of Biological Science Nara Institute of Science and TechnologyIkoma 630-0192 Japan Council for Agricultural research and Economics(CrEA) Research Centre for Genomics and Bioinformaticsvia Ardeatina 54600178 RomeItaly Universite de Toulouse INP-ENSA ToulouseGenomique et Biotechnologie des FruitsCastanet-Tolosan 31326France Institute of Plant Systems Biology 4000 PlovdivBulgaria Max Planck Institute for Biology of Ageing Joseph Stelzmann Strasse 9b50931 CologneGermany Institute of Botany and Molecular Genetics BioSCRWTH Aachen University52056 AachenGermany National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China

While the structures of plant primary metabolic pathways are generally well defined and highly conserved across species,those defining specialized metabolism are less well characterized and more highly variable across *** this study,we investigated polyphenolic metabolism in the lycopersicum complex by characterizing the underlying biosynthetic and decorative reactions that constitute the metabolic network of polyphenols across eight different species of *** this purpose,GC-MS-and LC-MS-based metabolomics of different tissues of Solatium lycopersicum and wild tomato species were carried out,in concert with the evaluation of cross-hybridized microarray data for MapMan-based transcriptomic analysis,and publicly available rNA-sequencing data for annotation of biosynthetic *** combined data were used to compile species-specific metabolic networks of polyphenolic metabolism,allowing the establishment of an entire pan-species biosynthetic framework as well as annotation of the functions of decoration enzymes involved in the formation of metabolic diversity of the flavonoid *** combined results are discussed in the context of the current understanding of tomato flavonol biosynthesis as well as a global view of metabolic shifts during fruit *** results provide an example as to how large-scale biology approaches can be used for the definition and refinement of large specialized metabolism pathways.

关键词： Solanumly copersicum secondary metabolism natural diversity wild accessions pathway elucidation gene discovery

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Two mitochondrial phosphatases,PP2c63 and Sal2,are required for posttranslational regulation of the TCA cycle in Arabidopsis

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Molecular Plant 2021年第7期14卷 1104-1118页

作者： Youjun Zhang Jonas Giese Sandra M.Kerbler Beata Siemiatkowska Leonardo Perez de Souza Jessica Alpers David Barbosa Medeiros Dirk K.Hincha Danilo M.Daloso Mark Stitt Iris Finkemeier alisdair r.fernie Max-Planck-lnstitut fur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria lnstitute of Plant Biology and Biotechnology University of MuensterSchlossplatz 748149 MuensterGermany Departamento de Bioquimica e Biologia Molecular Universidade Federal do CearaFortalezaCearaBrasil

Protein phosphorylation is a well-established post-translational mechanism that regulates protein functions and metabolic *** is known that several plant mitochondrial proteins are phosphorylated in a reversible ***,the identities of the protein kinases/phosphatases involved in this mech-anism and their roles in the regulation of the tricarboxylic acid(TCA)cycle remain *** this study,we isolated and characterized plants lacking two mitochondrially targeted phosphatases(Sal2 and PP2c63)along with pyruvate dehydrogenase kinase(PDK),Protein-protein interaction analysis,quantitative phos-phoproteomics,and enzymatic analyses revealed that PDK specifically regulates pyruvate dehydrogenase complex(PDC),while PP2c63 nonspecifically regulates *** recombinant PP2c63 and Sal2 proteins were added to mitochondria isolated from mutant plants,protein-protein interaction and enzymatic analyses showed that PP2c63 directly phosphorylates and modulates the activity of PDC,while Sal2 only indirectly affects TCA cycle *** of steady-state metabolite levels and fluxes in the mutant lines further revealed that these phosphatases regulate flux through the TCA cycle,and that altered metabolism in the sa/2 pp2c63 double mutant compromises plant *** results are discussed in the context of current models of the control of respiration in plants.

关键词： TCA cycle pyruvate dehydrogenase phosphorylation mitochondrial protein phosphatase TCA cycle enzymes phosphorylation

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Elucidation of the melitidin biosynthesis pathway in pummelo

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Journal of Integrative Plant Biology 2023年第11期65卷 2505-2518页

作者： Shuangqian Shen Shouchuang Wang Chenkun Yang Chao Wang Qianqian Zhou Shen Zhou ran Zhang Yufei Li Zixuan Wang Liupan Dai Wenjv Peng Yingchen Hao Hao Guo Guangping Cao Xianqing Liu Fan Yao Qiang Xu alisdair rfernie Jie Luo Sanya Nanfan research Institute of Hainan University Hainan UniversitySanya 572025China National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory College of Life Science and TechnologyCollege of Biomedicine and HealthHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Horticultural Plant Biology of Ministry of Education Huazhong Agricultural UniversityWuhan 430070China Max Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476Germany Centre of Plant Systems Biology and Biotechnology Plovdiv 4000Bulgaria Yazhouwan National Laboratory Sanya 572025China

Specialized plant metabolism is a rich resource of compounds for drug *** acylated flavonoid glycoside melitidin is being developed as an anti-cholesterol statin drug candidate,but its biosynthetic route in plants has not yet been fully ***,we describe the gene discovery and functional characterization of a new flavonoid gene cluster(UDP-glucuronosyltransferases(Cg UGTs),1,2rhamnosyltransferase(Cg1,2rha T),acyltransferases(Cg ATs))that is responsible for melitidin biosynthesis in pummelo(Citrus grandis(L.)Osbeck).Population variation analysis indicated that the tailoring of acyltransferases,specific for bitter substrates,mainly determine the natural abundance of ***,3-hydroxy-3-methylglutaryl-Co A reductase enzyme inhibition assays showed that the product from this metabolic gene cluster,melitidin,may be an effective anti-cholesterol statin drug ***-expression of these clustered genes in Nicotiana benthamiana resulted in the formation of melitidin,demonstrating the potential for metabolic engineering of melitidin in a heterologous plant *** study establishes a biosynthetic pathway for melitidin,which provides genetic resources for the breeding and genetic improvement of pummelo aimed at fortifying the content of biologically active metabolites.

关键词： bioactive metabolites biosynthetic pathway flavonoids melitidin natural variation

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An Oryza-specific hydroxycinnamoyl tyramine gene cluster contributes to enhanced disease resistance

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Science Bulletin 2021年第23期66卷 2369-2380页

作者： Shuangqian Shen Meng Peng Hong Fang Zixuan Wang Shen Zhou Xinyu Jing Meng Zhang Chenkun Yang Hao Guo Yufei Li Long Lei Yuheng Shi Yangyang Sun Xianqing Liu Congping Xu Takayuki Tohge Meng Yuan alisdair r.fernie Yuese Ning Guo-Liang Wang Jie Luo National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China College of Tropical Crops Hainan UniversityHaikou 570228China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology Ohio State UniversityColumbus OH 43210USA VIB-UGent Center for Plant Systems Biology Ghent UniversityGhent 9052Belgium Max Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476Germany Center of Plant Systems Biology and Biotechnology Plovdiv 4000Bulgaria Graduate School of Biological Sciences Nara Institute of Science and TechnologyIkoma Nara 630-0192Japan

Genomic clustering of non-homologous genes for the biosynthesis of plant defensive compounds is an emerging theme, but insights into their formation and physiological function remain limited. Here we report the identification of a newly discovered hydroxycinnamoyl tyramine(HT) gene cluster in *** cluster contains a pyridoxamine 50-phosphate oxidase(Os PDX3) producing the cofactor pyridoxal50-phosphate(PLP), a PLP-dependent tyrosine decarboxylase(Os Ty DC1), and two duplicated hydroxycinnamoyl transferases(Os THT1 and Os THT2). These members were combined to represent an enzymological innovation gene cluster. Natural variation analysis showed that the abundance of the toxic tyramine intermediate of the gene cluster among different rice accessions is mainly determined by the coordinated transcription of Os Ty DC1 and Os THT1. Further pathogen incubation assays demonstrated that the end products of the HT gene cluster displayed enhanced resistance to the bacterial pathogen Xanthomonas oryzae pv. Oryzae(Xoo) and fungal pathogen Magnaporthe oryzae(M. oryzae), and the enhanced resistance is associated with the boost of phytoalexins and the activation of defense response. The unique presence of the HT gene cluster in Oryza AA genome, together with the enrichment of transposon elements within this gene cluster region, provides an evolutionary background to accelerate cluster member combinations. Our study not only discovered a gene cluster involved in the phenylpropanoid metabolism but also addressed the key aspects of gene cluster formation. In addition, our results provide a new metabolic pool for plant defense against pathogens.

关键词： rice Gene cluster Hydroxycinnamic acid amide Phytoalexin Xanthomonas oryzae pv.oryzae Magnaporthe oryzae Transposon element

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