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Influence of fire on woody vegetation of savanna and forest formations in the Cerrado biome

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林业研究:英文版 2023年第5期34卷 1207-1216页

作者： aline das gracas Costa Fillipe Tamiozzo Pereira Torres Gumercindo souza Lima Fabiano Rodrigues de Melo Carlos Moreira Miquelino Eleto Torres Bruno Leao Said Schettini Vicente Paulo Santana Neto AndréLuiz Lopes de Faria Department of Forest Engineering Federal University of VicosaUFVVicosaMG 36570-900Brazil Department of Geography Federal University of VicosaUFVVicosaMG 36570-900Brazil

Wildfires have environmental,economic,and social impacts,and can shape the landscape and benefit ecosystems such as the *** study evaluated the diversity,similarity,and floristic and structural differences of woody savanna and forest formations of the Cerrado,when affected and not affected by ***-eight 25-400m2 plots were randomly allocated and divided into Burnt Cerrado and Unburnt Cerrado,Burnt Forest,and Unburnt Forest,and divided into three levels of inclusion according to diameter class(smaller than 2 cm,2-5 cm and larger than5 cm).Species were identified,DBH and height measured,and phyto sociological parameters such as volume,diversity,and floristic similarity *** Cerrado had lower diversity,density,and dominance at all inclusion levels compared to the Unburnt Cerrado,and showed similarities between treatments at the inclusion *** Forest had smaller differences in diversity,density,and dominance than Unburnt *** formation was similar at level 1 of inclusion and in the total ***,it was dissimilar at lower *** lower density and diversity of species at the lowest levels of inclusion was associated with mortality from fire.

关键词： Biodiversity Brazil Burning Floristic Phytosociology

Dipeptidyl peptidase 4(DPP4),adipose inflammation,and insulin resistance:is it time to look to the hepatocyte?

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Hepatobiliary Surgery and Nutrition 2018年第6期7卷 499-500页

作者： Wellington S.Silva Júnior Maria das gracas C.souza Luiz Guilherme Kraemer-Aguiar Endocrinology Discipline Faculty of MedicineCenter of NaturalHumanHealthand Technology SciencesFederal University of Maranhao(UFMA)PinheiroMABrazil Laboratory for Clinical and Experimental Research on Vascular Biology(BioVasc) Biomedical CenterState University of Rio de Janeiro(UERJ)Rio de JaneiroRJBrazil Postgraduate Program in Clinical and Experimental Physiopathology(FISCLINEX) Faculty of Medical SciencesUERJRio de JaneiroRJBrazil Endocrinology Department of Internal MedicineFaculty of Medical SciencesUERJRio de JaneiroRJBrazil

Dipeptidyl peptidase 4(DPP4),also known as adenosine deaminase binding protein or cluster of differentiation 26(CD26),is a serine exopeptidase able to inactivate peptides composed of proline,hydroxyproline,or alanine as the penultimate *** has a strong capacity to act in various peptides and is also widely expressed in many specialized cell types,such as endothelial cells,macrophages,and *** its physiological aspects,DPP4 inactivates the glucagon-like peptide-1(GLP-1),an incretin secreted by the gastrointestinal tract(1).Based on the antidiabetic actions of this incretin,several DPP4 inhibitors(named as gliptins)were launched in the market and are being in use for the treatment of type 2 diabetes(1).It is noteworthy that DPP4 also inactivate some cytokines,chemokines,and neuropeptides involved in inflammation,immunity,and vascular function(2).

关键词： inflammation immunity peptidase

^(11)C-PK11195 plasma metabolization has the same rate in multiple sclerosis patients and healthy controls:a cross-sectional study

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Neural Regeneration Research 2021年第12期16卷 2494-2498页

作者： aline Morais de souza Milena Sales Pitombeira Larissa Estessi de souza Fabio Luiz Navarro Marques Carlos Alberto Buchpiguel Caroline Cristiano Real Daniele de Paula Faria Laboratory of Nuclear Medicine(LIM43) Department of Radiology and OncologyFaculdade de Medicina FMUSPUniversidade de Sao PauloSao PauloSPBrazil Department of Neurology Faculdade de Medicina FMUSPUniversidade de Sao PauloSao PauloSPBrazil

^(11)C-PK11195 is a positron emitter tracer used for Positron Emission Tomography(PET)imaging of innate immune cell activation in studies of neuroinflammatory *** the image quantitative analysis,it is necessary to quantify the intact fraction of this tracer in the arterial plasma during imaging acquisition(plasma intact fraction).Due to the complexity and costs involved in this analysis it is important to evaluate the real necessity of individual analysis in each 11C-PK11195 PET imaging *** purpose of this study is to compare 11CPK11195 plasma metabolization rate between healthy controls and multiple sclerosis(MS)patients and evaluate the interference of sex,age,treatment,and disease phenotype in the tracer intact fraction measured in arterial plasma samples.11C-PK11195 metabolization rate in arterial plasma was quantified by high performance liquid chromatography in samples from MS patients(n=50)and healthy controls(n=23)at 20,45,and 60 minutes after 11C-PK11195 *** were also stratified by sex,age,treatment type,and MS *** results showed no significant differences in the metabolization rate of healthy controls and MS patients,or in the stratified *** conclusion,11C-PK11195 metabolization has the same rate in patients with MS and healthy controls,which is not affected by sex,age,treatment,and disease ***,these findings could contribute to exempting the necessity for tracer metabolization determination in all 11C-PK11195 PET imaging acquisition,by using a population metabolization rate *** study procedures were approved by the Ethics Committee for Research Projects Analysis of the Hospital das Clinicas of the University of Sao Paulo Medical School(approval No.624.065)on April 23,2014.

关键词： ^(11)C-PK11195 HPLC kinetic modeling metabolization multiple sclerosis neuroinflammation PET imaging PET tracer radiometabolites

Effects of aeration on the suspended matter from a tropical and eutrophic estuary

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Journal of Environmental Sciences 2019年第12期31卷 175-186页

作者： Natascha Krepsky Maria das gracas S.Bispo Luiz F.Fontana Frederico S.da Silva Jodo P.M.Torres Olaf Malm Cleverson G.Silva Mirian A.C.Crapez Laboratory of Marine Geology Fluminense Federal UniuersityAue.Gen.Milton Tauares de Souza s/n°-Gragoata24210-346 NiterdiRJBrazil Radioisotopes Laboratory Eduardo Penna Franca Biophysics Institute Carlos Chagas FilhoFederal University of Rio de JaneiroAu.Carlos Chagas Filho373 CCS-Bl.G21941-902 Rio de JaneiroRJBrazil Marine Biology Department Fluminense Federal UniversityR.Mario Santos Bragas/nNiteroi24.020-141 NiteroiRJBrazil

A comprehensive understanding of the complex biogeochemical interactions between organic matter and persistent contaminants in the suspended matter is vital for ecoefficient estuary ***,little is known regarding aeration effects in suspended particulate ***,this study aimed to investigate the effects of aeration on the suspended matter from a Tropical and Eutrophic estuarine *** water with 60 g/L of suspended particulate matter(SPM)was collected from Guanabara Bay,Rio de Janeiro,Brazil,transferred to experimental boxes and aerated for 61 *** aggregates monitoring included abiotic variables measurements and,determination of total organic matter(TOM),biopolymers composition,bacterial activity,trace metals,and polycyclic aromatic hydrocarbons(PAHs)*** aeration enhanced dissolved oxygen(DO)concentration and the redox potential(Eh).However,from days 0 to 61 the predominant bacterial activities were denitrification and *** transport system activity increased after day 10,and aerobic activity was detected after day *** summary,aeration increased aerobic bacterial activity,lipids(LIP)and trace metal concentrations,although diminished protein/carbohydrate ratio and PAH *** metals concentration(Ni,Pb,Cu,Cr,Mn,and Fe)were the highest on day 19 when the p H was *** presented toxic values(Cu>20.0μg/g).The p H showed a strong negative correlation with Eh(r=-0.94;p<0.001).Acidic environment(p H≤5.9)in marine ecosystems with high loads of toxic trace metals is unsafe for ***,managers must be aware of the environmental and biological risks of introducing the aeration technique into a eutrophic marine environment.

关键词： Mixed-pollution Remediation Acidification Esterases enzymes Biopolymer

Composition of Cachacas Produced from Five Varieties of Sugarcane and the Correlation of the Presence of Dhurrin in the Cane with That of Ethyl Carbamate in the Product

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American Journal of Plant Sciences 2019年第2期10卷 339-350页

作者： Francielli D’Carlos Cravo Wilder Douglas Santiago Allan da Silva Lunguinho Richard Bispo Barbosa Renan Elan da Silva Oliveira Gabriela Fontes Alvarenga Stephano Daniel Santos Rafaella Hilda Zaniti souza Ellen Cristina de souza Katia Julia de Almeida Josefina Aparecida de souza David Lee Nelson Maria das gracas Cardoso Department of Food Science Federal University of LavrasC.P.LavrasMinas GeraisBrazil Department of Chemistry Federal University of LavrasC.P.LavrasMinas GeraisBrazil Pro-Reitoria de Pesquisa e Pos-Graduacao Federal University of Vales de Jequitinhonha and MucuriDiamantinaMGBrazil

The present work sought to characterize the cyanogenic glycoside dhurrin in five sugarcane varieties and to determine its possible relationship with the formation of ethyl carbamate in cachaca. For each variety, methanol, ethyl acetate and hexane extracts were prepared and submitted to thin layer chromatography. Chromatographic spots were revealed with iodine vapors. The physical and chemical parameters of the spirits produced from the five different varieties of sugar cane and by different production processes were within the legal limits. Although not exceeding the limit for ethyl carbamate, a significant variation in the final concentration of this compound was observed for each sample analyzed. The Rf values for the dhurrin standard and for the sugarcane variety SP 83-2847 were equal, similar to those for SP 80-3280 and CTC 11 and different from the Rf values for the RB 86-7515 and IAC 86-2480 samples.

关键词： Cachaca Ethyl Carbamate Quality Glycosides Cyanide Dhurrin

Essential Oils from Mentha viridis(L).*** Mentha pulegium L.:Cytogenotoxic Effects on Human Cells

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American Journal of Plant Sciences 2017年第6期8卷 1423-1437页

作者： Lucilene Fernandes Silva Maria das gracas Cardoso Paulo Sérgio Castilho Preté Maria Luisa Teixeira David Lee Nelson Maísa Lamounier Magalhaes Vanuzia Rodrigues Fernandes Ferreira Rafaela Vieira souza Luana Isac Soares Silvana Marcussi Departamento de Química Universidade Federal de LavrasCampus UniversitárioLavrasMinas GeraisBrasil Departamento de Química Universidade Federal dos Vales do Jequitinhonha e MucuriCampus UniversitárioDiamantinaMinas GeraisBrasil Departamento de Ciência dos Alimentos Universidade Federal de LavrasCampus UniversitárioLavrasMinas GeraisBrasil

The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. These essential oils were able to inhibit the breakdown of phospholipids induced by venoms of snakes of the Bothrops genus. Both oils presented hemolytic activity, although the Mentha viridis (L). L. oil induced hemolysis only at the highest concentrations (14.6 and 29 μL·mL-1). The essential oil from M. viridis induced 3.9;8.6 and 16.2 times greater damage to human leukocyte DNA than that observed with the positive control (100 μg·μL-1 doxorubicin) at concentrations of 0.25;0.5 and 1.0 μL·mL-1, respectively. A similar effect was observed for the oil from M. pulegium (2.1, 2.5 and 15.8 times greater damage). The results extend the characterization of these essential oils and demonstrate their potential use in industries.

关键词： Essential Oils Mentha viridis(L).L. Mentha pulegium L. Hemolysis Comet Assay

Essential Oils from Mentha piperita,Cymbopogon citratus,Rosmarinus officinalis,Peumus boldus and Foeniculum vulgare:Inhibition of Phospholipase A_(2) and Cytotoxicity to Human Erythrocytes

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American Journal of Plant Sciences 2017年第9期8卷 2196-2207页

作者： Danubia Aparecida de Carvalho Selvati Rezende Maria das gracas Cardoso Rafaela Vieira souza Maria Luisa Teixeira Rafaela Magalhaes Brandao Vanuzia Rodrigues Fernandes Ferreira Jéssica Oliveira e Nogueira Maisa Lamounier Magalhaes Silvana Marcussi David Lee Nelson Departamento de Química Universidade Federal de Lavras Lavras Brasil Departamento de Ciência dos Alimentos Universidade Federal de Lavras Lavras Brasil Universidade Federal do Vale do Jequitinhonha e Mucuri Diamantina Brasil

The essential oils from Mentha piperita, Cymbopogon citratus, Rosmarinus officinalis, Peumus boldus and Foeniculum vulgare were extracted by hydrodistillation and characterized and quantified by GC-MS and GC-DIC. The oils induced hemolysis with all the doses evaluated (0.6 to 1.8 μL), and the diameters of the halos varied between 9 and 15 mm. Pre-incubation of P. boldus oil with Bothrops jararacussu venom resulted in potentiation of venom-induced hemolysis (30%) (proteases and phospholipases A2). The essential oil from M. piperita (0.6 μL) inhibited venom-induced hemolysis by 45%, whereas 0.6 μL of R. officinalis oil increased the hemolysis by 20%. For the essential oil from F. vulgare, 100% inhibition of activity (0.6 and 1.2 μL) was observed. The application of C. citratus oil induced hemolysis with all the volumes evaluated. Phospholipase activity induced by the venom was only inhibited (10%) with the 0.6 μL volume of R. officinalis oil. The oils from M. piperita and F. vulgare (1.8 μL) and C. citratus oil (0.6 μL) potentiated the phospholipase activity. The results highlight the need for a broad characterization and regulation of the use of natural products, because they can have therapeutic or toxic actions.

关键词： Natural Products Snake Venoms Hemolysis Pharmacological Potential

Influence of Cold Storage on the Bioactivity Properties and the Quality of the Juice of Moro Blood Orange(Citrus sinensis(L.)Osbeck)

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American Journal of Plant Sciences 2019年第1期10卷 24-37页

作者： Maisa Lamounier Magalhaes Luiz Carlos De Oliveira Lima Allan da Silva Lunguinho Danubia Aparecida De Carvalho Selvati Rezende Vanuzia Rodrigues Fernandes Ferreira Rafaela Magalhaes Brandao Josefina Aparecida De souza Ellen Cristina De souza Kátia Júlia De Almeida David Lee Nelson Maria das gracas Cardoso Departamento de Ciencia dos Alimentos Universidade Federal de Lavras(UFLA)LavrasBrazil Departamento de Química Universidade Federal de Lavras(UFLA)LavrasBrazil Programa de Pos-Graduacao em Biocombustívis Universidade Federal do Vale do Jequitinhonha e MucuriDiamantinaBrazil

The possibility of commercialization of Moro blood oranges in tropical countries such as Brazil was evaluated to verify whether post-harvest management through storage at low temperatures for a period of 60 days can improve the bioactive properties and quality parameters. Moro blood oranges cultivated in Brazil did not contain significant amounts of anthocyanins at the time of harvesting, but these compounds were activated by post-harvest management through storage at low temperatures (4°C and 8°C) for a period of 60 days. The emergence of the anthocyanins in the juices occurred within a few weeks of storage, but the maximum levels were attained after 60 days and at the temperature of 8°C. Cold storage positively influenced other bioactive compounds such as total phenolic compounds, individual phenolic compounds, β-carotene and the antioxidant activity determined by the sequestration of DPPH free radicals. It did not influence the vitamin C content. In addition, storage significantly altered the color, total acidity and pH of the fruits, but it did not prevent its commercial use. The remaining quality parameters were not influenced. It is possible to commercialize these oranges in Brazil through post-harvest management.

关键词： Cyanidin-3-Glycoside Antioxidant Post-Harvest

“Cachacas”(Sugarcane Spirit)Aged Quantitation of Phenolic Compounds,Antibacterial and Antioxidant Activity

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American Journal of Plant Sciences 2014年第20期5卷 2935-3942页

作者： Leonardo Milani Avelar Rodrigues Maria das gracas Cardoso Luís Roberto Batista Wilder Douglas Santiago Juliana Maria Valério Resende Juliana de Andrade Santiago Marcos de souza Gomes Milene Aparecida Andrade Maria Luisa Teixeira Fabiana Reinis Franca Passamani Department of Food Science Federal University of LavrasLavrasBrazil Department of Chemistry Federal University of LavrasLavrasBrazil

The “cachaça” is currently one of the fastest growing agro-industrial industries in the country as the drink considered as a symbol of Brazilian nationality. This study aimed to quantify the phenolic compounds, evaluate the antioxidant and antibacterial activity of different samples of aged “cachaça”. The physic-chemical analysis was performed at the Brandy Quality Laboratory of the Federal University of Lavras, and the determination of phenolic compounds was performed by High Performance Liquid Chromatography (HPLC). The antimicrobial activity evaluation was held at the Food Mycology Laboratory through the cavity agar diffusion technique and by steam using the micro-organisms Escherichia coli, Staphylococcus aureus, Salmonella choleraesuis, Listeria monocytogenes and Pseudomonas aeruginosa. The antioxidant activity was evaluated using the DPPH radical sequestering method. The values obtained for the phenolic compounds ranged from 0.41 to 9.69 mg/L, observing the predominance of syringaldehyde, gallic acid and vanillic acid. The phenolic extracts showed a satisfactory antibacterial activity for both Gram-negative and Gram-positive but did not show inhibition against the bacteria Pseudomonas aeruginosa. The antioxidant activity was evidenced by the DPPH test for cachaças tested and the “cachaça” aged in oak barrel showed the highest activity, followed by “cachaça” aged in jequitibá, amburana and balm barrels.

关键词： Syringaldehyde Gallic Acid Vanillic Acid Bacteria DPPH Test