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Fetal vs adult mesenchymal stem cells achieve greater gene expression, but less osteoinduction

World Journal of Stem Cells 2015年第1期7卷 223-234页

作者： Juan E Santiago-Torres Rebecca lovasz alicia l bertone Comparative Orthopedic Research laboratory Department of Veterinary Clinical Sciences College of Veterinary Medicine The Ohio State University Columbus OH 43210 United States College of Medicine The Ohio State University Columbus OH 43210 United States

AIM: To investigate adenoviral transduction in mesenchymal stem cells(MSCs) and effects on stemness in vitro and function as a cell therapy in ***: Bone marrow-derived adult and fetal MSC were isolated from an equine source and expanded in monolayer tissue culture. Polyethylenimine(PEI)-mediated transfection of pc DNA3-e GFP or adenoviral transduction of green fluorescent protein(GFP) was evaluated in fetal MSCs. Adenoviral-mediated transduction was chosen for subsequent experiments. All experiments were carried out at least in triplicate unless otherwise noted. Outcome assessment was obtained by flow cytometry or immunohystochemistry and included transduction efficiency, cell viability, stemness(i.e., cell proliferation, osteogenic and chondrogenic cell differentiation), and quantification of GFP expression. Fetal and adult MSCs were then transduced with an adenoviral vector containing the gene for the bone morphogenic protein 2(BMP2). In vitro BMP2 expression was assessed by enzyme linked immunosorbent assay. In addition, MSC-mediated gene delivery of BMP2 was evaluated in vivo in an osteoinduction nude mouse quadriceps model. New bone formation was evaluated by microradiography and ***: PEI provided greater transfection and viability in fetal MSCs than other commercial chemical reagents. Adenoviral transduction efficiency was superior to PEI-mediated transfection of GFP in fetal MSCs(81.3% ± 1.3% vs 35.0% ± 1.6%, P lar in adult MSCs(78.1% ± 1.9%). Adenoviral transduction provided significantly greater expression of GFP in fetal than adult MSCs(7.4 ± 0.1 vs 4.4 ± 0.3 millions of mean fluorescence intensity units, P ll as significantly greater in vitro BMP2 expression(0.16 pg/cell-day vs 0.10 pg/cell-day, P l MSC GFP positive cells decreased significantly faster than adult MSCs(1.15% ± 0.05% vs 11.4% ± 2.1% GFP positive at 2 wk post-transduction, P ll proliferation and osteo

关键词： Mesenchymal stem cells Biomarkers Next generation sequencing Diagnostic tool Tumor-initiating cells

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Accelerated chondrogenesis in nanofiber polymeric scaffolds embedded with BMP-2 genetically engineered chondrocytes

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Journal of Biomedical Science and Engineering 2010年第9期3卷 908-916页

作者： Robert T. Gorsline Prasam Tangkawattana John J. lannutti Mamoru Yamaguchi Christopher C. Kaeding alicia l. bertone 不详

This study evaluated chondrogenesis within a nanofiber polymeric scaffold seeded with isolated untreated chondrocytes, isolated chondrocytes genetically engineered with adenoviral (Ad) bone morphogenetic protein (BMP)-2, or isolated chondrocytes genetically engineered with green fluorescent protein (Ad-GFP). Electrospun polycaprolactone scaffolds (150-200 m thickness, 700 m fiber diameter, 30 m pore size) were optimally seeded with 1 x 107 isolated chondrocytes by using a 20% serum gradient culture system. Chondrocyte-scaffold constructs (untreated, Ad-B- MP-2 and Ad-GFP) were generated from 5 adult horses, cultured in triplicate for 7 or 14 days, and quantitatively analyzed for cell proliferation (DNA content;Hoechst assay), viability, morphology (confocal microscopy), matrix production (proteoglycan content;DMMB assay), and mRNA expression of collagen I, collagen II, and aggrecan. Chondrocytes transduced with Ad-BMP-2 demonstrated greater cell numbers and significantly greater expression of chondrogenic markers including aggrecan, collagen II, and proteoglycan through 14 days of culture as compared to untransduced or Ad-GFP controls. This study demons- trated that chondrocytes can be driven to seed a polycaprolactone nanofiber scaffold by serum gradient and a polycaprolactone nanofiber scaffold containing Ad-BMP2 transduced chondrocytes resulted in grea- ter and accelerated chondrogenesis than controls. This cell engineered construct has potential use in one-step cartilage repair in vivo.

关键词： Nanofiber Scaffold Chondrogenesis BMP- 2 Adenovirus

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Assessment of SARS-CoV-2 airborne infection transmission risk in public buses

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Geoscience Frontiers 2022年第6期13卷 224-235页

作者： M.bertone A.Mikszewski l.Stabile G.Riccio G.Cortellessa F.R.d'Ambrosio V.Papa l.Morawska G.Buonanno Department of Civil and Mechanical Engineering University of Cassino and Southern LazioCassinoFRItaly CIUS Building Performance lab The City University of New YorkNew YorkNYUSA International laboratory for Air Quality and Health Queensland University of TechnologyBrisbaneQueenslandAustralia Department of Industrial Engineering University of Naples“Federico II”Italy Department of Industrial Engineering University of SalernoItaly Department of Motor Sciences and Wellness University of Naples“Parthenope”Italy

Public transport environments are thought to play a key role in the spread of SARS-CoV-2 ***,high crowding indexes(*** numbers of people relative to the vehicle size),inadequate clean air supply,and frequent extended exposure durations make transport environments potential hotspots for transmission of respiratory *** the COVID-19 pandemic,generic mitigation measures(*** distancing)have been applied without also considering the airborne transmission *** is due to the lack of quantified data about airborne contagion risk in transport *** this study,we apply a novel combination of close proximity and room-scale risk assessment approaches for people sharing public transport environments to predict their contagion risk due to SARS-CoV-2 respiratory *** particular,the individual infection risk of susceptible subjects and the transmissibility of SARS-CoV-2(expressed through the reproduction number)are evaluated for two types of buses,differing in terms of exposure time and crowding index:urban and long-distance *** risk and reproduction number are calculated for different scenarios as a function of the ventilation rates(both measured and estimated according to standards),crowding indexes,and travel *** results show that for urban buses,the close proximity contribution significantly affects the maximum occupancy to maintain a reproductive number oflar,full occupancy of the bus would be permitted only for an infected subject breathing,whereas for an infected subject speaking,masking would be *** long-distance buses,full occupancy of the bus can be maintained only if specific mitigation solutions are simultaneously *** example,for an infected person speaking for 1 h,appropriate filtration of the recirculated air and simultaneous use of FFP2 masks would permit full occupancy of the bus for a period of almost 8 ***,a high percentage of immunized persons(>

关键词： SARS-CoV-2 Airborne transmission Bus Transport microenvironment Maximum occupancy Air exchange rate

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A novel mathematical template for developing fDOM probe fluorescence signal correction models for freshwaters

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Journal of Environmental Sciences 2024年第12期146卷 103-117页

作者： Hiua Daraei Edoardo bertone John Awad Rodney A.Stewart Christopher W.K.Chow Jinming Duan Amanda Mussared John Van leeuwen Sustainable Infrastructure and Resource Management(SIRM) UniSA STEMUniversity of South AustraliaMawson LakesSA 5095Australia Griffith School of Engineering and Built Environment Griffith UniversityQueensland 4222Australia Cities Research Institute Griffith UniversityParklands DriveSouthportQueensland 4222Australia CSIRO Environment AdelaideSA 5000Australia Australian Water Quality Centre SA Water250 Victoria SquareAdelaideSA 5000Australia Australian Rivers Institute Griffith University170 Kessels RoadNathanQueensland 4111Australia Environmental Health Research Centre Kurdistan University of Medical SciencesSanandajKurdistanIran

The reliable application of field deployable fluorescent dissolved organic matter(fDOM)probes is hindered by several influencing factors which need to be *** manuscript describes the corrections of temperature,pH,turbidity and inner filter effect on fluorescence signal of a commercial fDOM probe(fDOMs).For this,Australian waters with wide ranging qualities were selected,*** organic carbon(DOC)ranging from∼1 to∼30 mg/l,specific UV absorbance at 254 nm from∼1 to∼6 l/m/mg and turbidity from∼1 to∼350 ***-based model calibration experiments(MCEs)were performed.A model template was developed and used for the development of the correction *** each factor,data generated through MCEs were used to determine model coefficient(α)values by fitting the generated model to the experimental *** discrete factor models were generated by determination of a factor-specificα***αvalues derived for each water of the MCEs subset were consistent for each factor *** indicated generic nature of the fourαvalues across wide-ranging water *** correlation between fDOMs and DOC were achieved after applying the four-factor compensation models to new data(r,0.96,plso,average biases(and%)between DOC predicted through fDOMs and actual DOC were decreased by applying the four-factor compensationmodel(from3.54(60.9%)to 1.28(16.7%)mg/l DOC).These correction models were incorporated into a Mi-crosoft EXCEl-based software termed EXOf-Correct for ready-to-use applications.

关键词： Surface waters Online monitoring fDOM probes Fluorescence signal Model-based corrections

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一周岁母牛的膀胱破裂一例

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江西畜牧兽医杂志 1985年第3期 44-45页

作者：刘鑫 A.l.bertone

在纽约州兽医内科学院的兽医教学医院,有一头一岁龄,400公斤的黑白花母牛,两天来的病史为进行性减食和腹痛。兽医诊断为外伤性网——腹膜炎,并用促反刍药物(Ruminatorics)、磁铁和青霉素进行治疗。小母牛精神沉郁,厌食和不愿行走。两侧... 详细信息

在纽约州兽医内科学院的兽医教学医院,有一头一岁龄,400公斤的黑白花母牛,两天来的病史为进行性减食和腹痛。兽医诊断为外伤性网——腹膜炎,并用促反刍药物(Ruminatorics)、磁铁和青霉素进行治疗。小母牛精神沉郁,厌食和不愿行走。两侧下腹部中等度膨胀。心率高(104次/分),呼吸和直肠温度正常,四肢冰冷和中等度脱水。听诊无瘤胃收缩音,且瘤胃背侧有一气帽,尿流缺乏。

关键词：脓毒性腹膜炎母牛代谢性紊乱

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Electron-ion collider in China

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Frontiers of physics 2021年第6期16卷 1-78页

作者： Daniele PAnderle Valerio bertone Xu Cao lei Chang Ningbo Chang Gu Chen Xurong Chen Zhuojun Chen Zhufang Cui lingyun Dai Weitian Deng Minghui Ding Xu Feng Chang Gong longcheng Gui Feng-Kun Guo Chengdong Han Jun He Tie-Jiun Hou Hongxia Huang Yin Huang KrešImir KumeričKi lPKaptari Demin li Hengne li Minxiang li Xueqian li Yutie liang Zuotang liang Chen liu Chuan liu Guoming liu Jie liu liuming liu Xiang liu Tianbo liu Xiaofeng luo Zhun lyu Boqiang Ma Fu Ma Jianping Ma Yugang Ma lijun Mao Cédric Mezrag HervéMoutarde Jialun Ping Sixue Qin Hang Ren Craig DRoberts Juan Rojo Guodong Shen Chao Shi Qintao Song Hao Sun PawełSznajder Enke Wang Fan Wang Qian Wang Rong Wang Ruiru Wang Taofeng Wang Wei Wang Xiaoyu Wang Xiaoyun Wang Jiajun Wu Xinggang Wu lei Xia Bowen Xiao Guoqing Xiao Ju-Jun Xie Yaping Xie Hongxi Xing Hushan Xu Nu Xu Shusheng Xu Mengshi Yan Wenbiao Yan Wencheng Yan Xinhu Yan Jiancheng Yang Yi-Bo Yang Zhi Yang Deliang Yao Zhihong Ye Peilin Yin C-PYuan Wenlong Zhan Jianhui Zhang Jinlong Zhang Pengming Zhang Yifei Zhang Chao-Hsi Chang Zhenyu Zhang Hongwei Zhao Kuang-Ta Chao Qiang Zhao Yuxiang Zhao Zhengguo Zhao liang Zheng Jian Zhou Xiang Zhou Xiaorong Zhou Bingsong Zou liping Zou Guangdong Provincial Key laboratory of Nuclear Science Institute of Quantum MatterSouth China Normal UniversityGuangzhou 510006China IRFU CEAUniversitéParis-SaclayF-91191 Gif-sur-YvetteFrance Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China University of Chinese Academy of Sciences Beijing 100049China Nankai University Tianjin 300071China Xinyang Normal University Xinyang 464000China School of Physics and Materials Science Guangzhou UniversityGuangzhou 510006China Hunan University Changsha 410082China Nanjing University Nanjing 210093China Huazhong University of Science and Technology Wuhan 430074China European Centre for Theoretical Studies in Nuclear Physics and Related Areas(ECT*)and Fondazione Bruno Kessler Villa TambosiStrada delle Tabarelle 286I-38123 Villazzano(TN)Italy School of Physics Peking UniversityBeijing 100871China Hunan Normal University Changsha 410081China Institute of Theoretical Physics Chinese Academy of SciencesBeijing 100190China Nanjing Normal University Nanjing 210023China Department of Physics College of SciencesNortheastern UniversityShenyang 110819China Southwest Jiaotong University Chengdu 610000China Department of Physics Faculty of ScienceUniversity of ZagrebBijenička c.3210000 ZagrebCroatia Bogoliubov laboratory of Theoretical Physics Joint Institute for Nuclear ResearchDubna 141980Russia School of Physics and Microelectronics Zhengzhou UniversityZhengzhou 450001China lanzhou University Lanzhou 730000China Key laboratory of Particle Physics and Particle Irradiation(MOE) Shandong UniversityQingdao 266237China Key laboratory of Quark and lepton Physics(MOE)and Institute of Particle Physics Central China Normal UniversityWuhan 430079China School of Physics Southeast UniversityNanjing 211189China Key laboratory of Nuclear Physics and Ion-beam Application(MOE) Institute of Modern PhysicsFudan UniversityShanghai 200433China Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Depa

lepton scattering is an established ideal tool for studying inner structure of small particles such as nucleons as well as *** a future high energy nuclear physics project,an Electron-ion collider in China(EicC)has been *** will be constructed based on an upgraded heavy-ion accelerator,High Intensity heavy-ion Accelerator Facility(HIAF)which is currently under construction,together with a new electron *** proposed collider will provide highly polarized electrons(with a po-larization of 80%)and protons(with a polarization of 70%)with variable center of mass energies from 15 to 20 GeV and the luminosity of(2–3)×1033 cm^(−2)·s^(−1).Polarized deuterons and Helium-3,as well as unpolarized ion beams from Carbon to Uranium,will be also available at the *** main foci of the EicC will be precision measurements of the structure of the nucleon in the sea quark region,including 3D tomography of nucleon;the partonic structure of nuclei and the parton interaction with the nuclear environment;the exotic states,especially those with heavy flavor quark *** addition,issues fundamental to understanding the origin of mass could be addressed by measurements of heavy quarkonia near-threshold production at the *** order to achieve the above-mentioned physics goals,a hermetical detector system will be constructed with cutting-edge *** document is the result of collective contributions and valuable inputs from experts across the *** EicC physics program complements the ongoing scientific programs at the Jefferson laboratory and the future EIC project in the United *** success of this project will also advance both nuclear and particle physics as well as accelerator and detector technology in China.

关键词： electron ion collider nucleon structure nucleon mass exotic hadronic states quantum chromodynamics 3D-tomography helicity transverse momentum dependent parton distribution generalized parton distribution energy recovery linac polarization spin rotator

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马大结肠变位的手术治疗

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国外兽医学（畜禽疾病） 1993年第2期14卷 13-16页

作者： Rose P l 许万祥

bertone等(1986,1987)论述了采用大结肠切除和吻合术作为大结肠不同疾病(包括扭转)的外科校正方法。

关键词：马病大结肠外科手术

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水稻回交群体农艺性状的遗传变异及产量分析

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河南农业科学 2020年第11期49卷 10-18页

作者：田蓉蓉白天亮马帅国吕建东胡慧朱春艳李杰王震宇田蕾李培富宁夏大学农学院/宁夏优势特色作物现代分子育种重点实验室宁夏银川750021

为了解水稻生长发育过程中农艺性状的遗传变异及其对单株产量的影响,利用bertone和越光为亲本构建回交群体,对群体株高、穗长、茎基粗、剑叶长、剑叶宽、单株分蘖数、单株有效穗数、单穗质量和单株产量9个性状进行测定。结果表明,上述9... 详细信息

为了解水稻生长发育过程中农艺性状的遗传变异及其对单株产量的影响,利用bertone和越光为亲本构建回交群体,对群体株高、穗长、茎基粗、剑叶长、剑叶宽、单株分蘖数、单株有效穗数、单穗质量和单株产量9个性状进行测定。结果表明,上述9个性状均呈连续性变异,单株产量和单穗质量在2个回交群体中均表现出较高的变异;单株产量与单株分蘖数、单株有效穗数和单穗质量均呈极显著正相关,与株高、茎基粗均呈显著正相关。通过主成分分析将9个性状转换为5个主成分,累计贡献率为88.980%;结合主成分和逐步回归分析获得了单株产量、单穗质量、单株有效穗数、株高和剑叶长5个关键性状指标;通径分析发现,单穗质量对单株产量的直接作用最大,上述5个关键指标对单株产量的间接作用较小。遗传方差分析发现,不同性状的遗传方差分量组成差异很大,且不同分量占总方差的比例也不同,显性与环境互作方差是回交群体中主要的遗传方差来源。

关键词：水稻回交群体农艺性状产量主成分分析通径分析遗传方差

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水稻回交群体剑叶性状综合评价及QTl定位

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中国水稻科学 2021年第6期35卷 573-585页

作者：李杰田蓉蓉白天亮朱春艳宋佳伟田蕾马帅国吕建东胡慧王震宇罗成科张银霞李培富宁夏大学农学院/宁夏优势特色作物现代分子育种重点实验室银川750021 白银市农业农村局宁夏白银730900

【目的】通过对水稻剑叶性状的综合评价,明确剑叶相关性状间及与6个农艺性状的关系。检测剑叶相关性状的QTl,为优良株型品种选育,剑叶性状基因的精细定位和克隆奠定基础。【方法】以日本优质粳稻品种越光和葡萄牙粳稻地方种bertone构建... 详细信息

【目的】通过对水稻剑叶性状的综合评价,明确剑叶相关性状间及与6个农艺性状的关系。检测剑叶相关性状的QTl,为优良株型品种选育,剑叶性状基因的精细定位和克隆奠定基础。【方法】以日本优质粳稻品种越光和葡萄牙粳稻地方种bertone构建的回交群体两个世代为实验材料,利用BC3F1群体基因型构建遗传连锁图谱;测定亲本和BC3F2群体各株系剑叶SPAD、剑叶长、剑叶宽,计算剑叶长宽比、剑叶面积;利用隶属函数和标准差系数赋予权重法获得剑叶性状综合评价值(D值),分析其与6个农艺性状间的关系。分别利用单标记分析(SPA)和区间作图(IM)检测水稻剑叶相关性状QTl。【结果】在抽穗灌浆期,两亲本剑叶SPAD值呈现先升高后降低的动态变化。BC3F2群体的5个剑叶相关性状变异丰富,总体表现趋向轮回亲本越光。4个剑叶形态性状间相关性均达到极显著水平,与剑叶SPAD的相关性不显著。主成分和逐步线性回归分析表明剑叶宽、剑叶SPAD、剑叶长、剑叶面积是影响剑叶综合评价值(D值)的主要因子。高D值株系的株高、穗长、茎基粗和单株产量均极显著高于低D值株系,两者的分蘖数和有效穗数差异不显著。共检测到18个控制剑叶性状的QTl,分布在水稻第1、4、7和8染色体上,贡献率分布范围为4.00%~28.00%(SPA)和3.41%~27.00%(IM),除qFlSPAD1之外的17个QTl增效基因均来自bertone。在第8染色体上的RM22720-RM404区间发现1个QTl簇,含6个主效QTl,分别为q Fll8.1、q Fll8.2、qFlA8.1、q FlA8.2、qD8.1和q D8.2。【结论】获得了剑叶宽、剑叶SPAD、剑叶长和剑叶面积4个评价剑叶性状的关键指标;明确了剑叶性状与单株产量之间的正相关关系;检测到18个剑叶相关性状QTl,位于第8染色体RM22720-RM404区间的QTl簇,是影响剑叶性状的1个重要染色体区域。

关键词：水稻回交群体剑叶性状综合评价 QTl定位

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NaCl胁迫对不同耐盐性粳稻种质幼苗叶绿素荧光特性的影响

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中国农业科学 2022年第13期55卷 2509-2525页

作者：朱春艳宋佳伟白天亮王娜马帅国普正菲董艳吕建东李杰田蓉蓉罗成科张银霞马天利李培富田蕾宁夏大学农学院/宁夏优势特色作物现代分子育种重点实验室中航(宁夏)生物股份有限公司塔里木大学农学院

【目的】叶绿素荧光可反映盐胁迫下植物光合机构防御机制的受害程度和抗逆性。通过分析盐胁迫对不同耐盐性粳稻种质叶绿素荧光特性的影响，揭示其诱导动力学特征，初步阐明叶绿素荧光相关基因调控粳稻种质苗期耐盐性的机制，为耐盐水稻... 详细信息

【目的】叶绿素荧光可反映盐胁迫下植物光合机构防御机制的受害程度和抗逆性。通过分析盐胁迫对不同耐盐性粳稻种质叶绿素荧光特性的影响，揭示其诱导动力学特征，初步阐明叶绿素荧光相关基因调控粳稻种质苗期耐盐性的机制，为耐盐水稻品种筛选和培育提供理论依据。【方法】以 8 份耐盐、8 份盐敏感粳稻种质为试验材料，于苗期在水培条件下分别测定两组种质 0 mmol·l-1和 125 mmol·l-1Na Cl 胁迫 3 d、6 d 的叶片叶绿素荧光参数。通过主成分分析筛选关键指标，利用隶属函数和标准差系数赋予权重法综合评价各种质资源耐盐性，获得典型耐(敏)盐种质开展叶绿素荧光相关基因 OsHCF222和 OsABCI7 的表达特异性分析。【结果】与对照(CK,0 mmol·l-1NaCl 3 d、6 d)相比，盐胁迫(125 mmol·l-1NaCl 3 d、6 d)可显著降低粳稻种质的最大荧光产量(Fm)、原初光能转化效率(Fv/Fm)。与 CK 相比，耐盐种质的非光化学荧光淬灭系数(NPQ)和可变荧光的非光化学猝灭系数(qN)在盐胁迫 3 d 时显著降低，初始荧光产量(Fo)在盐胁迫 6 d 显著升高；盐敏感种质的光化学淬灭系数(qP)和 qN 在盐胁迫 3 d 时显著降低，实际光化学量子产量(Y)、NPQ、表观光合电子传递速率(ETR)在盐胁迫 3 d、6 d 时极显著降低。盐胁迫下 Fm、Fv/Fm、Y、NPQ 和 ETR 与耐盐级别(STS)均呈极显著正相关，且在耐、敏盐种质间差异显著。通过主成分分析将 8 个叶绿素荧光参数转换为 2 个主成分，累积贡献率 88.018%；结合各因子载荷大小筛选出 Fm、Fv/Fm、Y、NPQ 和 ETR 5 个关键指标，可将 16 份粳稻种质明确划分为耐盐组和盐敏感组两类。以两个主成分的隶属函数结合权重处理并累加计算盐胁迫下叶绿素荧光特性综合评价值 D(DCF)，并依此获得 16 份粳稻种质排名。采用 Kinetic 模式测定盐胁迫和正常生长(CK)条件下耐盐种质 Cigalon、bertone 和盐敏感种质新竹 8号、幸实的叶绿素荧光诱导动力学曲线。在 CK 条件下，4 份粳稻种质表现为相似的曲线形状，斜率较大，P 峰出现时间基本相同；盐胁迫下，随着盐胁迫时间的延长，盐敏感种质的 P 峰迅速降低，M 峰和曲线斜率逐渐变小；耐盐种质仍维持较高的 P 峰，M 峰和曲线斜率与 CK 相比无明显变化。通过对综合排名第 1 的耐盐种质 Cigalon 和排名第 16 的盐敏感种质幸实 NaCl 胁迫不同时间叶绿素荧光相关基因 OsHCF222 和 OsABCI7 的 qPCR 分析，结合叶绿素含量、耐盐相关叶绿素荧光参数的动态变化和相关分析，初步明确了 OsHCF222 和 OsABCI7 的差异表达与粳稻种质苗期耐盐性的关系。【结论】不同耐盐性粳稻种质的叶绿素荧光参数对盐胁迫的响应不尽相同，Fm、Fv/Fm、Y、NPQ 和 ETR 与水稻苗期耐盐性密切相关；OsHCF222和 OsABCI7 的表达量直接影响了粳稻种质苗期耐盐性；在耐盐粳稻中，NPQ 和 Fv/Fm起关键作用，Fm可能在调节盐敏感粳稻耐盐性中发挥重要作用。

关键词：粳稻盐胁迫叶绿素荧光参数诱导动力学曲线实时荧光定量PCR

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