Accelerated chondrogenesis in nanofiber polymeric scaffolds embedded with BMP-2 genetically engineered chondrocytes
Accelerated chondrogenesis in nanofiber polymeric scaffolds embedded with BMP-2 genetically engineered chondrocytes作者机构：不详
出 版 物：《Journal of Biomedical Science and Engineering》 (生物医学工程（英文）)
年 卷 期：2010年第3卷第9期
页 面：908-916页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Nanofiber Scaffold Chondrogenesis BMP- 2 Adenovirus
摘 要：This study evaluated chondrogenesis within a nanofiber polymeric scaffold seeded with isolated untreated chondrocytes, isolated chondrocytes genetically engineered with adenoviral (Ad) bone morphogenetic protein (BMP)-2, or isolated chondrocytes genetically engineered with green fluorescent protein (Ad-GFP). Electrospun polycaprolactone scaffolds (150-200 m thickness, 700 m fiber diameter, 30 m pore size) were optimally seeded with 1 x 107 isolated chondrocytes by using a 20% serum gradient culture system. Chondrocyte-scaffold constructs (untreated, Ad-B- MP-2 and Ad-GFP) were generated from 5 adult horses, cultured in triplicate for 7 or 14 days, and quantitatively analyzed for cell proliferation (DNA content;Hoechst assay), viability, morphology (confocal microscopy), matrix production (proteoglycan content;DMMB assay), and mRNA expression of collagen I, collagen II, and aggrecan. Chondrocytes transduced with Ad-BMP-2 demonstrated greater cell numbers and significantly greater expression of chondrogenic markers including aggrecan, collagen II, and proteoglycan through 14 days of culture as compared to untransduced or Ad-GFP controls. This study demons- trated that chondrocytes can be driven to seed a polycaprolactone nanofiber scaffold by serum gradient and a polycaprolactone nanofiber scaffold containing Ad-BMP2 transduced chondrocytes resulted in grea- ter and accelerated chondrogenesis than controls. This cell engineered construct has potential use in one-step cartilage repair in vivo.
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