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HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis

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Plant Communications 2023年第3期4卷 244-257页

作者： Silvina Mangano alfonso Muñoz Lourdes fernández-Calvino M.Mar Castellano Centro de Biotecnología y Genómica de Plantas Universidad Politécnica de Madrid(UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria(INIA/CSIC)Campus Montegancedo UPM28223 Pozuelo de Alarcón(Madrid)Spain Fundación Instituto Leloir and Instituto de Investigaciones Bioquímicas de Buenos Aires(IIBA CONICET)Av.Patricias Argentinas 435Buenos Aires C1405BWEArgentina Departamento de Botánica Ecologíay Fisiología VegetalCampus de RabanalesEdificio Severo OchoaUniversidad de Córdoba14071 CórdobaSpain

Gibberellins(GAs)play important roles in multiple developmental processes and in plant response to the *** the GA pathway,a central regulatory step relies on GA-dependent degradation of the DELLA transcriptional ***,the relevance of the stability of other key proteins in this pathway,such as SLY1 and SNE(the F-box proteins involved in DELLA degradation),remains ***,we take advantage of mutants in the HSP70-HSP90 organizing protein(HOP)co-chaperones and reveal that these proteins contribute to the accumulation of SNE in ***,HOP proteins,along with HSP90 and HSP70,interact in vivo with SNE,and SNE accumulation is significantly reduced in the hop ***,greater accumulation of the DELLA protein RGA is observed in these *** agreement with these molecular phenotypes,hop mutants show a hypersensitive response to the GA inhibitor paclobutrazol and display a partial response to the ectopic addition of GA when GA-regulated processes are *** mutants also display different phenotypes associated with alterations in the GA pathway,such as reduced germination rate,delayed bolting,and reduced hypocotyl elongation in response to warm ***,ectopic overexpression of SNE reverts the delay in germination and the thermally dependent hypocotyl elongation defect of the hop1 hop2 hop3 mutant,revealing that SNE accumulation is the key aspect of the hop mutant ***,these data reveal a pivotal role for HOP in SNE accumulation and GA signaling.

关键词： HSP70-HSP90 organizing protein seed germination thermomorphogenesis RGA protein folding protein stability

Seven-year follow-up of posterior chamber phakic intraocular lens with central port design

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Eye and Vision 2021年第1期8卷 223-231页

作者： Luis fernández-Vega-Cueto Belén alfonso-Bartolozzi Carlos Lisa David Madrid-Costa JoséF.alfonso fernández-Vega Ophthalmological Institute Avda.Dres.Fernández-Vega 11433012 OviedoSpain Optometry and Vision Department Faculty of Optics and OptometryUniversity Complutense de MadridMadridSpain

Background:To assess the clinical outcomes of the Visian Implantable Collamer Lens(ICL)with a central port throughout 7 years of ***:Eighty-four eyes of 52 patients were evaluated over a follow-up period of 7 years after V4c ICL ***(UDVA)and corrected(CDVA)distance visual acuities,refraction,intraocular pressure(IOP),endothelial cell density(ECD)and vault were ***:The mean postoperative UDVA(logMAR)was 0.04±0.11,0.13±0.19 and 0.17±0.23 at 1-,5-and 7-years,respectively(P20 mmHg or an increase higher than 5 mmHg)occurred in any case throughout the 7-year of *** loss in ECD from the preoperative baseline at the last follow-up visit was 2.6%.No intraoperative or postoperative complications or adverse events occurred during the follow-up ***:The outcomes of this study show the long-term viability of the V4c ICL implantation as a surgical option for the correction of myopia.

关键词： Implantable Collamer Lens ICL Myopia Long-term

Planetary Gear Profile Modification Design Based on Load Sharing Modelling

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Chinese Journal of Mechanical Engineering 2015年第4期28卷 810-820页

作者： IGLESIAS Miguel fernández DEL RINCóN alfonso DE-JUAN Ana Magdalena GARCIA Pablo DIEZ Alberto VIADERO fernando Mechanical Engineering Research Group University of Cantabria

In order to satisfy the increasing demand on high performance planetary transmissions, an important line of research is focused on the understanding of some of the underlying phenomena involved in this mechanical system. Through the development of models capable of reproduce the system behavior, research in this area contributes to improve gear transmission insight, helping developing better maintenance practices and more efficient design processes. A planetary gear model used for the design of profile modifications ratio based on the levelling of the load sharing ratio is presented. The gear profile geometry definition, following a vectorial approach that mimics the real cutting process of gears, is thoroughly described. Teeth undercutting and hypotrochoid definition are implicitly considered, and a procedure for the incorporation of a rounding arc at the tooth tip in order to deal with corner contacts is described. A procedure for the modeling of profile deviations is presented, which can be used for the introduction of both manufacturing errors and designed profile modifications. An easy and flexible implementation of the profile deviation within the planetary model is accomplished based on the geometric overlapping. The contact force calculation and dynamic implementation used in the model are also introduced, and parameters from a real transmission for agricultural applications are presented for the application example. A set of reliefs is designed based on the levelling of the load sharing ratio for the example transmission, and finally some other important dynamic factors of the transmission are analyzed to assess the changes in the dynamic behavior with respect to the non-modified case. Thus, the main innovative aspect of the proposed planetary transmission model is the capacity of providing a simulated load sharing ratio which serves as design variable for the calculation of the tooth profile modifications.

关键词： transmission planetary gear load sharing relief profile modification

Facial nerve palsy following parotid gland surgery:A machine learning prediction outcome approach

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2023年第4期9卷 271-279页

作者： Carlos M.Chiesa‐Estomba Jose A.gonzález‐García Ekhiñe Larruscain Jon A.Sistiaga Suarez Miquel Quer Xavier León Paula Martínez‐Ruiz de Apodaca Celia López‐Mollá Miguel Mayo‐Yanez alfonso Medela Department of Otorhinolaryngology—Headand Neck Surgery Donostia University HospitalDonosti‐San SebastiánSpain Head&Neck Study Group of Young‐Otolaryngologists of the International Federations of Oto‐rhino‐laryngological Societies(YO‐IFOS) ParisFrance Biodonostia Health Research Institute San SebastiánSpain Department of Otorhinolaryngology Hospital Santa Creu I Sant PauUniversitatAutònoma de BarcelonaBarcelonaSpain Department of Otorhinolaryngology Doctor Peset University HospitalValenciaSpain Otorhinolaryngology—Head and Neck Surgery Department Complexo Hospitalario Universitario A Coruña(CHUAC)A CoruñaGaliciaSpain Clinical Research in Medicine International Center for Doctorate and Advanced Studies(CIEDUS)Universidade de Santiago deCompostela(USC)Santiago de CompostelaGaliciaSpain LEGIT Health BilbaoSpain

Introduction:Machine learning (ML)‐based facial nerve injury (FNI) forecasting grounded on multicentric data has not been released up to *** distinct ML models,random forest (RF),K‐nearest neighbor,and artificial neural network (ANN),for the prediction of FNI were evaluated in this ***:A retrospective,longitudinal,multicentric study was performed,including patients who went through parotid gland surgery for benign tumors at three different university ***:Seven hundred and thirty‐six patients were *** most compelling aspects related to risk escalation of FNI were as follows:(1) location,in the mid‐portion of the gland,near to or above the main trunk of the facial nerve and at the top part,over the frontal or the orbital branch of the facial nerve;(2) tumor volume in the anteroposterior axis;(3) the necessity to simultaneously dissect more than one level;and (4) the requirement of an extended resection compared to a lesser extended *** contrast,in accordance with the ML analysis,the size of the tumor (>3 cm),as well as gender and age did not result in a determining favor in relation to the risk of ***:The findings of this research conclude that ML models such as RF and ANN may serve evidence‐based predictions from multicentric data regarding the risk of ***:Along with the advent of ML technology,an improvement of the information regarding the potential risks of FNI associated with patients before each procedure may be achieved with the implementation of clinical,radiological,histological,and/or cytological data.

关键词： gland machine learning parotid personalized medicine surgery

Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality

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Journal of Animal Science and Biotechnology 2023年第1期14卷 114-133页

作者： Cláudia Lima Verde Leal Karina Canón-Beltrán Yulia N.Cajas Meriem Hamdi Aracelli Yaryes María Gemma Millán de la Blanca Paula Beltrán-Brena Rosane Mazzarella Juliano Coelho da Silveira alfonso Gutiérrez-Adán Encina M gonzález Dimitrios Rizos Department of Animal Reproduction National Center Institute for Agriculture and Food Research and Technology(CSIC-INIA)28040 MadridSpain. Departamento de Medicina Veterinaria Faculdade de Zootecnia e Engenharia de AlimentosUniversidade de Sao Paulo(FZEA-USP)PirassunungaBrazil. Facultad de Ciencias Agrarias y Ambientales Programa de Medicina VeterinariaFundacion Universitaria Juan de CastellanosTunjaColombia. Laboratorio de Biotecnologia de la Reproduccion Animal Facultad de Ciencias AgropecuariasUniversidad de Cuenca(UC)EC010205 CuencaEcuador. Department of Anatomy and Embryology Veterinary Faculty-Universidad Complutense de Madrid(UCM)MadridSpain.

Background:In vitro production of bovine embryos is a well-established technology,but the in vitro culture(IVC)system still warrants improvements,especially regarding embryo *** study aimed to evaluate the effect of extracellular vesicles(EVs)isolated from oviductal(OF)and uterine fluid(UF)in sequential IVC on the development and quality of bovine *** were cultured in SOF supplemented with either BSA or EVs-depleted fetal calf serum(dFCS)in the presence(BSA-EV and dFCS-EV)or absence of EVs from OF(D1 to D4)and UF(D5 to D8),mimicking in vivo *** from oviducts(early luteal phase)and uterine horns(mid-luteal phase)from slaughtered heifers were isolated by size exclusion *** rate was recorded on days 7-8 and their quality was assessed based on lipid contents,mitochondrial activity and total cell numbers,as well as survival rate after *** mRNA abundance for lipid metabolism-related transcripts and levels of phosphorylated hormonesensitive lipase(pHSL)proteins were also ***,the expression levels of 383 miRNA in OF-and UF-EVs were assessed by ***:Blastocyst yield was lower(P0.05).Lipid metabolism transcripts were affected by EVs and showed interaction with type of protein source in medium(PPARGC1B,LDLR,CD36,FASN and PNPLA2,P<0.05).Levels of pHSL were lower in dFCS(P<0.05).Twenty miRNA were differentially expressed between OF-and UF-EVs and only bta-miR-148b was increased in OF-EVs(P<0.05).Conclusions:Mimicking physiological conditions using EVs from OF and UF in sequential IVC does not affect embryo development but improves blast

关键词： Cattle Cryopreservation Embryo development Exosomes Lipid metabolism miRNAs Oviduct Uterus

Dominant Macroalgae in Bahía de Cochinos, Matanzas, Cuba

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American Journal of Plant Sciences 2022年第6期13卷 797-814页

作者： Yusimí alfonso Patricia gonzález Sánchez Hansel Caballero Aragón Pedro Chevalier Jhoana Díaz-Larrea Ruben Cabrera Acuario Nacional de Cuba La Habana Cuba Unidad Iztapalapa Departamento de Hidrobiología Universidad Autónoma Metropolitana Cd de México México Gabinete de Arqueología Oficina del Historiador de la Ciudad Habana Vieja Cuba

Spatial-temporal variations of macroalgae were analyzed in a study conducted in Cueva de Los Peces (CP) and Punta Perdiz (PP), two dive sites located on the eastern coast of Bahía de Cochinos. The most conspicuous species found from 3 to 20 m depths were present in the biotopes of ridges and shallow and deep terraces. Sampling was carried out in September 2014, and March and October 2016 by autonomous diving and direct methods. Qualitative visual censuses and quantitative analyzes were used to estimate the coverage (%) of the genera according to the AGRRA methodology with the use of the 10 m linear transect and quadrats as the sampling unit. The first list of macroalgae for the area is offered that includes 49 taxa of which 10 were Rhodophyta, 10 Ochrophyta (Phaeophyceae), and 29 Chlorophyta. The dominant genera during the study period were Halimeda, Dictyota, Lobophora, and Udotea. Spatial differences were found regarding the specific composition. Temporal variations were evidenced in terms of the relative abundance of the genera present, which reveals temporal changes in the qualitative structure, where some species replace others. The information obtained is pioneering and can serve as a comparative baseline for future monitoring of the area.

关键词： Cuba Macroalgae Spatial-Temporal Variations Species List

Artificial muscles driven by the cooperative actuation of electrochemical molecular *** discrepancies and challenges

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International Journal of Smart and Nano Materials 2017年第4期8卷 125-143页

作者： Toribio fernández Otero Laboratory of Electrochemistry Intelligent Materials and DevicesUniversidad Politécnica de Cartagena.Aulario IIICampus Alfonso XIIICartagenaSpain

Here we review the persisting conceptual discrepancies between different research groups working on artificial muscles based on conducting polymers and other electroactive *** basic question is if they can be treated as traditional electro-mechanical(physical)actuators driven by electric fields and described by some adaptation of their physical models or if,replicating natural muscles,they are electro-chemo-mechanical actuators driven by electrochemical reaction of the constitutive molecular machines:the polymeric *** that case the charge consumed by the reaction will control the volume variation of the muscular material and the motor displacement,following the basic and single Faraday’s laws:the charge consumed by the reaction determines the number of exchanged ions and solvent,the film volume variation to lodge/expel them and the amplitude of the *** from the linear relationships are due to the osmotic exchange of solvent and to the presence of parallel reactions from the electrolyte,which originate creeping *** and limitations are underlined.

关键词： Electromechanical actuator electro-chemo-mechanical artificial muscle molecular machines creeping osmotic effects

Emergence of bariatric psychiatry as a new subspecialty

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World Journal of Psychiatry 2022年第1期12卷 108-116页

作者： alfonso Troisi Department of Systems Medicine University of Rome Tor VergataRome 00133Italy

Bariatric surgery is the branch of surgery aimed at helping a person with obesity lose *** implementation of surgical treatment of obesity is growing at an impressive *** expected,the expanding implementation of bariatric procedures has progres-sively revealed critical issues that were not evident when the number of obese patients treated with surgery was relatively *** critical issue is the importance of mental health assessment and care of bariatric *** aim of this review is to provide readers with an up-to-date summary of the goals,methods,and clinical strategies of bariatric *** aims can be grouped into three distinct ***,to ascertain that there are no psychiatric contraindications to safe bariatric ***,to diagnose and treat pre-surgery mental conditions that could predict poor weight ***,to diagnose and treat post-surgery mental conditions associated with poor quality of *** bariatric psychiatry has gained the status of a new subspecialty within the field of mental health and psychopathology,many clinical questions remain *** need more long-term data on outcome measures such as quality of life,adherence to behavioral guidelines,risk of suicide,and postsurgery prevalence of psychological disturbances and mental disorders.

关键词： Bariatric surgery Psychiatry Weight loss Mental health Quality of life Preoperative assessment Postoperative follow-up

Inventory and Geometrical Changes in Small Glaciers Covering Three Northern Patagonian Summits Using Remote Sensing and GIS Techniques

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Journal of Mountain Science 2010年第1期7卷 26-35页

作者： alfonso fernández José Araos Jorge Marín Department of Geography Faculty of Architecture Urbanism and Geography Universidad de Concepción Centro de Estudios Avanzados en Zonas áridas (Ceaza)

In the last few decades, a large quantity of research has been performed to elucidate the current behavior of glaciers in southern Chile, especially with respect to the volumetric changes in the outlets of the Northern and Southern Patagonian Icefields (NPI and SPI, respectively). Calculations have shown a generalized thinning and withdrawal, which greatly contributes to the increase in sea level attributed to the ice melt from non-polar glaciers. However, these icefields are surrounded by many small icecaps, which have yet to be studied in detail. A precise estimation of the volume of ice located in these mountain chains could provide new information with respect to this area's exact contribution to the increase in sea level. Thus, this study presents an inventory of relatively small Northern Patagonian glaciers in the surrounding of the three summits: Mount Queulat, and the Macá and Hudson volcanoes. The study used remote sensing techniques in a GIS environment to determine the margins, surface areas, thickness changes and hypsometry for the glaciated zones. Landsat images from different dates were analyzed using standard band ratio and screen delineation techniques. Additionally, digital elevation models from different dates were compared using map algebra, calculating thickness changes. Based on the results, we propose that there are important volumetric changes in the glaciers studied, which could be explained by precipitation trends in a general context, and an influence of the glaciers' sizes in some local response. Therefore, we suggest the exact contribution of the Patagonia to the increase in sea level corresponds to a regional pattern rather than just the behavior of a single ice field.

关键词：冰川变化遥感技术 GIS技术库存地理信息系统海平面上升塔几何