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Neural Regeneration Research 2012年第29期7卷 2273-2278页

作者： Igor Papalia Giulia Ronchi Luisa Muratori alessandra Mazzucco Ludovico Magaudda Stefano Geuna Department of Biomorphology and Biotechnologies University of MessinaMessina 98100Italy Neuroscience Institute of the Cavalieri Ottolenghi Foundation (NICO)&Department of Clinical and Biological Sciences University of TurinTorino 10043Italy

The need for the continuous research of new tools for improving motor function recovery after nerve injury is justified by the still often unsatisfactory clinical outcome in these patients. It has been previously shown that the combined use of two reconstructive techniques, namely end-to-side neurorrhaphy and direct muscle neurotization in the rat hindlimb model, can lead to good results in terms of skeletal muscle reinnervation. Here we show that, in the rat forelimb model, the combined use of direct muscle neurotization with either end-to-end or end-to-side neurorrhaphy to reinnervate the denervated flexor digitorum muscles, leads to muscle atrophy prevention over a long postoperative time lapse （10 months）. By contrast, very little motor recovery （in case of end-to-end neurorrhaphy） and almost no motor recovery （in case of end-to-side neurorrhaphy） were observed in the grasping activity controlled by flexor digitorum muscles. It can thus be concluded that, at least in the rat, direct muscle neurotization after both end-to-end and end-to-side neurorrhaphy represents a good strategy for preventing denervation-related muscle atrophy but not for regaining the lost motor function.

关键词： nerve injury nerve reconstruction microsurgery axonal regeneration denervation muscle atrophy Schwann cells stereology grasping test rats

Effects of CXCL12 isoforms in a pancreatic pre-tumour cellular model:Microarray analysis

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World Journal of Gastroenterology 2021年第15期27卷 1616-1629页

作者： Monia Cecati Matteo Giulietti alessandra Righetti Berina Sabanovic Francesco Piva Department of Specialistic Clinical and Odontostomatological Sciences Polytechnic University of MarcheAncona 60126Italy

BACKGROUND Pancreatic ductal adenocarcinoma(PDAC)is the fourth leading cause of death among cancers,it is characterized by poor prognosis and strong *** the PDAC microenvironment,stromal cells release different extracellular components,including *** CXCL12 is a chemokine promoting the communication between tumour and stromal *** different splicing isoforms of CXCL12 are known(α,β,γ,δ,ε,θ)but their role in PDAC has not yet been *** To investigate the specific role ofα,β,andγCXCL12 isoforms in PDAC *** We used hTERT-HPNE E6/E7/KRasG12D(Human Pancreatic Nestin-Expressing)cell line as a pancreatic pre-tumour model and exposed it to theα,β,andγCXCL12 *** altered expression profiles were assessed by microarray analyses and confirmed by Real-Time polymerase chain *** functional enrichment analyses have been performed by Enrichr tool to highlight Gene Ontology enriched *** addition,wound healing assays have been carried out to assess the phenotypic changes,in terms of migration ability,induced by theα,β,andγCXCL12 *** Microarray analysis of hTERT-HPNE cells treated with the three different CXCL12 isoforms highlighted that the expression of only a few genes was ***,theαandβisoforms showed an alteration in expression of different genes,whereasγisoform affected the expression of genes also common withαandβ***βisoform altered the expression of genes mainly involved in cell cycle *** addition,all isoforms affected the expression of genes assay confirmed that CXCL12 enhanced the migration ability of hTERT-HPNE *** the CXCL12 splicing isoforms,theγisoform showed higher induction of migration thanαandβ*** Our data suggests an involvement and different roles of CXCL12 isoforms in PDAC ***,more investigations are needed to confirm these preliminary observations.

关键词： CXCL12 Splicing isoforms Pancreatic cancer Microarray Migration Wound healing assay

Intestinal complications in patients with Crohn's disease in the Brazilian public healthcare system between 2011 and 2020

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World Journal of Clinical Cases 2023年第14期11卷 3224-3237页

作者： Ligia Yukie Sassaki Adalberta Lima Martins Rodrigo Galhardi-Gasparini Rogerio Saad-Hossne alessandra Mileni Versut Ritter Tania Biatti Barreto Taciana Marcolino Bruno Balula Claudia Yang-Santos Department of Internal Medicine São Paulo State University-UNESPMedical School18618687BotucatuBrazil Department of Gastroenterology State Office for Pharmaceutical Assistance at Espírito Santo Health OfficeVitoria 29017-010Espirito SantoBrazil Department of Gastroenterology SETE–Specialized Medical CenterMarilia 17502-020Sao PauloBrazil Department of Surgery São Paulo State University-UNESPMedical School18618687BotucatuBrazil Real World Evidence IQVIA Brazil04719-002Sao PauloBrazil Medical Affairs Takeda Pharmaceuticals Brazil04794-000Sao PauloBrazil Clinical Research Takeda Pharmaceuticals Brazil04794-000Sao PauloBrazil

BACKGROUND This is a secondary database study using the Brazilian public healthcare system *** To describe intestinal complications(ICs)of patients in the Brazilian public healthcare system with Crohn’s disease(CD)who initiated and either only received conventional therapy(CVT)or also initiated anti-tumor necrosis factor(anti-TNF)therapy between 2011 and *** This study included patients with CD[international classification of diseases–10th revision(ICD-10):K50.0,K50.1,or K50.8](age:≥18 years)with at least one claim of CVT(sulfasalazine,azathioprine,mesalazine,or methotrexate).IC was defined as a CD-related hospitalization,pre-defined procedure codes(from rectum or intestinal surgery groups),and/or associated disease(pre-defined ICD-10 codes),and overall(one or more type of ICs).RESULTS In the 16809 patients with CD that met the inclusion criteria,the mean follow-up duration was 4.44(2.37)*** total,14697 claims of ICs were found from 4633 *** the 1-and 5-year of follow-up,8.3%and 8.2%of the patients with CD,respectively,presented at least one IC,of which fistula(31%)and fistulotomy(48%)were the most commonly *** overall incidence rate(95%CI)of ICs was 6.8(6.5–7.04)per 100 patient years for patients using only-CVT,and 9.2(8.8–9.6)for patients with evidence of anti-TNF *** The outcomes highlighted an important and constant rate of ICs over time in all the CD populations assessed,especially in patients exposed to anti-TNF *** outcome revealed insights into the real-world treatment and complications relevant to patients with CD and highlights that this disease remains a concern that may require additional treatment strategies in the Brazilian public healthcare system.

关键词： Crohn’s disease Intestinal complications Anti-tumor necrosis factor Conventional therapy Public healthcare system

A multi-agent system for itinerary suggestion in smart environments

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CAAI Transactions on Intelligence Technology 2021年第4期6卷 377-393页

作者： alessandra De Paola Salvatore Gaglio Andrea Giammanco Giuseppe Lo Re Marco Morana Universita degzi studi di Palermo PalermoItaly Srart Cities and Comtnunitics Natioral Jab CINI-Consorzio Interuniversitario Nazionale per l'Infortnatica PalerinoItaly

Modem smart environments pose several challenges,among which the design of intelligent algorithms aimed to assist the *** a variety of points of interest are available,for instance,trajectory recommendations are needed to suggest users the most suitable itineraries based on their interests and contextual *** in many cases,these interests must be explicitly requested and their lack causes the so-called cold-start ***,lengthy travelling distances and excessive crowdedness of specific points of interest make itinerary planning more *** address these aspects,a multi-agent itinerary suggestion system that aims at assisting the users in an online and collaborative way is proposed.A profiling agent is responsible for the detection of groups of users whose movements are characterised by similar semantic,spatial and temporal features;then,a recommendation agent leverages contextual information and dynamically associates the current user with the trajectory clusters according to a Multi-Armed Bandit policy;Framing the trajectory recommendation as a reinforcement learning problem permits to provide high-quality suggestions while avoiding both cold-start and preference elicitation *** effectiveness of the approach is demonstrated by some deployments in real-life scenarios,such as smart campuses and theme parks.

关键词： artificial intelligence pattern recognition

Immune response to vaccines in children with celiac disease

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World Journal of Gastroenterology 2017年第18期23卷 3205-3213页

作者： Caterina Anania Francesca Olivero alessandra Spagnolo Claudio Chiesa Lucia Pacifico Policlinico Umberto I Sapienza University of Rome Department of Public Health and Infectious Diseases Sapienza University of Rome Institute of Translational Pharmacology National Research Council

Celiac disease(CD) is an immune-mediated systemic condition evoked by ingestion of gluten and related prolamines in genetically susceptible subjects. The disease is featured by a variable combination of clinical signs, specific antibodies, HLA-DQ2 and HLA-DQ8 haplotypes, and enteropathy. Vaccination is the most potent intervention for infectious disease prevention. Several factors including age, gender, ethnicity, quality and quantity of vaccine antigen, doses, and route of administration can influence immune response to vaccination, although the main cause of variation in the responsiveness among vaccine recipients is host genetic variability. The HLA system has a fundamental role in identifying the antigens introduced into the host with the vaccines and in the development of specific antibodies, and some HLA phenotypes have been associated with a less effective immunological response. The available literature indicates that the immunological response to vaccines in CD children does not differ markedly from that of general population and antibody titres are high enough to provide long-term protection, except for hepatitis B virus vaccine. In this article, we review and discuss the scarce literature in this field in order to provide clinical practice guidelines to achieve the most efficient monitoring of the response to vaccines in pediatric CD patients.

关键词：腹的疾病孩子感染疫苗肝炎 B 疫苗 HLA 面筋免费饮食

To compute or not to compute?

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Journal of Traffic and Transportation Engineering(English Edition) 2019年第1期6卷 85-93页

作者： alessandra Fiore Ivo Vanzi Camillo Nuti Cristoforo Demartino Rita Greco Bruno Briseghella Department of Engineering and Geology University of Chieti-Pescara "G.d'Annunzio" DICAR Department of Civil Engineering and Architecture Polytechnic University of Bari College of Civil Engineering Fuzhou University Department of Architecture Roma Tre University College of Civil Engineering Nanjing Tech University DICATECH Department of Civil Engineering Environment Territory Building and Chemical Polytechnic University of Bari

In a previous paper "to retrofit or not to retrofit?"(Nuti and Vanzi, 2003) a straightforward procedure able to forecast the economic return of seismic structural upgrading was presented. More recently, the authors realized that the final mathematical results can be much simplified so as to allow back-of-an-envelope computation. The title of this paper tries to highlight precisely this aspect, namely that for many a regular seismic structural upgrading cases, nearly no computation is needed(apart from one subtraction and one multiplication) to assess their economic convenience. These findings are presented and discussed in this paper, together with a state of the art on the cost-studies available in literature and technical codes. The mathematical formulation leading to the proposed approximation is suitably explained, underlining its applicability field and comparing it with the rigorous solution. Also a table and a formula are furnished that alternatively allows to calculate the maximum estimation errors, in order to obtain an upper and lower bound for the maximum amount of money which should be allocated for seismic structural upgrading.

关键词： Seismic retrofitting Structural reliability Safety Optimization Cost minimization

Hypoallergenicity of a thickened hydrolyzed formula in children with cow’s milk allergy

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World Journal of Clinical Cases 2019年第16期7卷 2256-2268页

作者： Danilo Rossetti Salvatore Cucchiara alessandra Morace Beatrice Leter Salvatore Oliva Department of Pediatrics Sapienza University of Rome

BACKGROUND Allergy to cow’s milk is the most frequent allergy occurring in infants and young *** dietary management of these patients consists of the elimination of any cow’s milk proteins from the diet,and for formula-fed infants,the substitution of the usual infant formula with an adapted formula that is generally based on extensively hydrolyzed cow’s milk *** American Academy of Pediatrics has established specific criteria to confirm the hypoallergenicity of a formula intended for these *** To assess the hypoallergenicity of a new thickened extensively hydrolyzed casein-based formula (TeHCF) in children with cow’s milk allergy (CMA).METHODS Children diagnosed with CMA through a double-blind placebo-controlled food challenge (DBPCFC) were randomly administered increased doses of a placebo formula or the TeHCF [Allernova,new thickener including fibres (Novalac)] under double-blind conditions and medical surveillance on two separate ***,both of these formulas and a cow’s milk-based formula were randomly introduced to children who were highly suspected of having CMA on three separate *** and late reactions occurring after the introduction of any of these formulas were thoroughly recorded by the physician at the hospital and reported by parents to the physician after hospital discharge,*** the children tolerated the TeHCF during the DBPCFC,they were exclusively fed this formula during a 3-mo period where potential allergic symptoms,anthropometric parameters,as secondary outcomes,and adverse events were *** Cow’s Milk-related Symptoms Score (CoMiSSTM) was assessed and anthropometric parameters were compared to World Health Organization (WHO) reference *** Of the 30 children included in the study,the CMA diagnosis of 29 (mean age: 8.03 ± 7.43 mo) patients was confirmed by a *** children all tolerated the TeHCF during both the challenge and the subsequent 3-mo feeding period,which the

关键词： Cow's milk allergy Hypoallergenicity Tolerance Thickened extensively hydrolyzed formula Digestive comfort Dietary management

Molecular epidemiology and phylogeny of Nipah virus infection:A mini review

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Asian Pacific Journal of Tropical Medicine 2016年第7期9卷 610-615页

作者： Silvia Angeletti alessandra Lo Presti Eleonora Cella Massimo Ciccozzi Unit of Clinical Pathology and Microbiology University Campus Bio-Medico of Rome Department of Infectious Parasitic and Immunomediated Diseases Istituto Superiore di Sanità Public Health and Infectious Diseases Sapienza University

Nipah virus(Ni V) is a member of the genus Henipavirus of the family Paramyxoviridae,characterized by high pathogenicity and endemic in South *** is classified as a Biosafety Level-4(BSL-4) *** case-fatality varies from 40%-70% depending on the severity of the disease and on the availability of adequate healthcare *** present no antiviral drugs are available for Ni V disease and the treatment is just *** and evolutionary analyses can be used to help in understanding the epidemiology and the temporal origin of this *** review provides an overview of evolutionary studies performed on Nipah viruses circulating in different *** phylogenetic studies have been published from 2000 to 2015 years,searching on pub-med using the key words ‘Nipah virus AND phylogeny' and twenty-eight molecular epidemiological studies from 2006 to 2015 have been performed,typing the key words ‘Nipah virus AND molecular epidemiology'.Overall data from the published study demonstrated as phylogenetic and evolutionary analysis represent promising tools to evidence NiV epidemics,to study their origin and evolution and finally to act with effective preventive measure.

关键词： Nipah virus Phylogenetic analysis Molecular epidemiology NiV reservoir

Least speciose among the most speciose:Natural history correlates of monospecific and bispecific genera of Rodentia and Soricomorpha

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Integrative Zoology 2017年第6期12卷 489-499页

作者： Giovanni AMORI alessandra Maria BISSATTINI Spartaco GIPPOLITI Leonardo VIGNOLI Luigi MAIORANO Luca LUISELLI CNR Institute of Ecosystem StudiesRomeItaly Department of Science University Roma TreRomeItaly SocietàItaliana di Storia della Fauna RomeItaly Department of Biology and Biotechnology Charles Darwin Sapienza UniversityRomeItaly Department of Applied and Environmental Biology Rivers State University of Science and TechnologyPort HarcourtNigeria and Institute for DevelopmentEcologyConservation and CooperationRomeItaly

Monospecific and bispecific genera are of special concern as they represent unique phylogenetic/evolutionary trajectories within larger *** addition,as phylogenetically older taxa are supposed to be exposed to higher rarity and extinction risk,monospecific and bispecific genera may be intrinsically more prone to extinction risks than multispecies genera,although extinction risks also depend on the ecological and biological strategy of the ***,the distribution across biogeographical zones and the levels of threat to 2 speciose orders of mammals(monospecific and bispecific genera of Rodentia and Soricomorpha)are investigated in order to highlight major patterns at the worldwide *** Rodentia,39.7%of the genera(n=490)were monospecific and 17.9%were *** Soricomorpha,44.4%of the total genera(n=45)were monospecific and 15%were *** was a positive correlation between the number of monospecific genera and the total number of genera per *** of monospecific and bispecific genera richness were observed in Neotropical,Oriental and Afrotropical regions in rodents and in the Palearctic region in *** size was significantly uneven across biogeographic region in rodents(with larger ranges in Nearctic and Oriental regions and smaller ranges in the Australian region),but there was no difference across biogeographic regions in terms of range size in *** of the monospecific and bispecific genera occurred in forest habitat in both *** frequency distribution of the monospecific and bispecific genera across IUCN categories did not differ significantly from the expected pattern using the total rodent genera and the multispecies genera.

关键词： bispecific genera conservation monospecific genera Rodentia Soricomorpha

Microtubule Depolymerization Affects Endocytosis and Exocytosis in the Tip and Influences Endosome Movement in Tobacco Pollen Tubes

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Molecular Plant 2013年第4期6卷 1109-1130页

作者： Aurora Irene Idilli Piero Morandini Elisabetta Onelli Simona Rodighiero Marco Caccianiga alessandra Moscatelli Department of Biosciences Milan University Via Celoria 26 20133 Milan Italy Fondazione Filarete Milan University Viale Ortles 22 Milan Italy

Polarized organization of the cytoplasm of growing pollen tubes is maintained by coordinated function of actin filaments （AFs） and microtubules （MTs）. AFs convey post-Golgi secretory vesicles to the tip where some fuse with specific domains of the plasma membrane （PM）. Secretory activity is balanced by PM retrieval that maintains cell mem- brane economy and regulates the polarized composition of the PM, by dividing lipids/proteins between the shank and the tip. Although AFs play a key role in PM internalization in the shank, the role of MTs in exo-endocytosis needs to be characterized. The present results show that integrity of the MT cytoskeleton is necessary to control exo-endocytosis events in the tip. MT polymerization plays a role in promoting PM invagination in the apex of tobacco pollen tubes since nocodazole affected PM internalization in the tip and subsequent migration of endocytic vesicles from the apex for degradation. MT depolymerization in the apex and shank was associated with misallocation of a significantly greater amount of internalized PM to the Golgi apparatus and its early recycling to the secretory pathway. Fluorescence Recovery After Photobleaching （FRAP） experiments also showed that MT depolymerization in the tip region influenced the rate of exocytosis in the central domain of the apical PM.

关键词： pollen tube microtubules endocytosis exocytosis.