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Hydrothermal carbonization of livestock mortality for the reduction of pathogens and microbially-derived DNA

Frontiers of Environmental Science & Engineering 2017年第3期11卷 107-114页

作者： Thomas F. Ducey Jessica C. Collins Kyoung S. ro Bryan L. Woodbury D. Dee Griffin Coastal Plains Soil Water and Plant Research Center Agricultural Research Service United States Department of Agriculture Florence SC 29501 USA South Carolina Governor's School for Science and Mathematics Hartsville SC 29550 USA U.S. Meat Animal Research Center Agricultural Research Service United States Department of Agriculture Clay Center NE 68933 USA University of Nebraska-Lincoln Great Plains Veterinary Education Center Lincoln NE 68588 USA

Hydrothermal carbonization （HTC）, utilizing high temperature and pressure, has the potential to treat agricultural waste via inactivating pathogens, antibiotic resistance genes （ARG）, and contaminants of emerging concern （CEC） in a environmental and economical manner. Livestock mortality is one facet of agricultural waste that can pose a threat to the surrounding environment. While several methods are utilized to treat livestock mortality, there remains a paucity of data on the elimination of microbially- derived DNA in these treatment practices. This DNA, most notably ARGs, if it survives treatment can be reintroduced in agricultural environments where it could potentially be passed to pathogens, posing a risk to animal and human populations. HTC treatments have been successfully utilized for the treatment of CECs, however very little is understood on how ARGs survive HTC treatment. This study aims to fill this knowledge gap by examining the survivability ofmicrobially-derived DNA in the HTC treatment of livestock mortality. We examined three treatment temperatures （100℃, 150℃, and 200℃） at autogenic pressures at three treatment times （30, 60, and 240 min）. We examined the amplification of a plasmid-bome reporter gene carried by Escherichia coli DH 10B introduced to both beef bone and tissue. Results indicate that while all three temperatures, at all treatment times, were suitable for complete pathogen kill, only temperatures of 150℃ and 200℃ were sufficient for eliminating microbial DNA. These results serve as the basis for future potential HTC treatment recommendations for livestock mortality when considering the elimination of pathogens and ARGs.

关键词： High-temperature carbonization Microbial DNA Livestock mortality

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Cutaneous Dengue Virus Inoculation Triggers Strong B Cell Reactions but Contrastingly Poor T Cell Responses

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Virologica Sinica 2020年第5期35卷 575-587页

作者： Edith marcial-Juárez Julio García-Cordero Raúl Antonio Maqueda-Alfaro Rafael Eduardo Saucedo-López Luvia Enid Sánchez-Torres Leticia Cedillo-Barrón Leopoldo Flores-romo Department of Cell Biology Center for Advanced Research(CINVESTAV-IPN)The National Polytechnic Institute07360 Mexico CityMexico Department of Molecular Biomedicine Center for Advanced Research(CINVESTAV-IPN)The National Polytechnic Institute07360 Mexico CityMexico Immunology Department The National School of Biological Sciences(ENCB-IPN)The National Polytechnic Institute11340 Mexico CityMexico

Dengue is a global health problem without current specific treatment nor safe vaccines *** severe dengue is related to pre-existing non-neutralizing dengue virus(DENV)antibodies,the role of T cells in protection or pathology is *** cutaneous DENV infection in immunocompetent mice we previously showed the generation of PNA+germinal centers(GCs),now we assessed the activation and proliferation of B and T cells in draining lymph nodes(DLNs).We found a drastic remodelling of DLN compartments from 7 to 14 days post-infection(dpi)with greatly enlarged B cell follicles,occupying almost half of the DLN area compared to*24%in na?ve *** clusters of proliferating(Ki-67+)cells inside B follicles were found 14 dpi,representing*33%of B cells in DLNs but only*2%in noninfected *** GCs,we noticed an important recruitment of tingle body macrophages removing apoptotic *** contrast,the percentage of paracortex area and total T cells decreased by 14–16 dpi,compared to *** randomly distributed Ki-67+T cells were found,similar to non-infected ***69 expression by CD4+and CD8+T cells was minor,while it was remarkable in B cells,representing 1764.7%of change from basal levels 3 *** apparent lack of T cell responses cannot be attributed to apoptosis since no significant differences were observed compared to noninfected *** study shows massive B cell activation and proliferation in DLNs upon DENV *** contrast,we found very poor,almost absent CD4+and CD8+T cell responses.

关键词： Dengue virus(DENV) B and T cells proliferation Lymphocyte activation Lymph nodes In vivo cutaneous infection Immunocompetent mice

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Solvent-free fabrication of broadband WS2 photodetectors on paper

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Opto-Electronic Advances 2023年第3期6卷 1-11页

作者： Wenliang Zhang Onur Çakıroğlu Abdullah Al-Enizi Ayman Nafady Xuetao Gan Xiaohua Ma Sruthi Kuriakose Yong Xie Andres Castellanos-Gomez Materials Science Factory Instituto de Ciencia de Materiales de Madrid(ICMM-CSIC)Madrid E-28049Spain Department of Chemistry College of ScienceKing Saud UniversityRiyadh 11451Saudi Arabia Key Laboratory of Light Field Manipulation and Information Acquisition Ministry of Industry and Information Technologyand Shaanxi Key Laboratory of Optical Information TechnologySchool of Physical Science and TechnologyNorthwestern Polytechnical UniversityXi’an 710129China School of Advanced Materials and Nanotechnology Xidian UniversityXi’an 710071China

Paper-based devices have attracted extensive attention due to the growing demand for disposable flexible ***,we integrate semiconducting devices on cellulose paper substrate through a simple abrasion technique that yields high-performance photodetectors.A solvent-free WS_(2) film deposited on paper favors an effective electron-hole separation and hampers *** as-prepared paper-based WS2 photodetectors exhibit a sensitive photoresponse over a wide spectral range spanning from ultraviolet(365 nm)to near-infrared(940 nm).Their responsivity value reaches up to~270 mA W^(−1) at 35 V under a power density of 35 mW cm^(−2).A high performance photodetector was achieved by controlling the environmental exposure as the ambient oxygen molecules were found to decrease the photoresponse and stability of the WS_(2) ***,we have built a spectrometer using such a paperbased WS_(2) device as the photodetecting component to illustrate its potential *** present work could promote the development of cost-effective disposable photodetection devices.

关键词： paper electronics photodetector van der Waals materials solvent-free deposition tungsten disulfide

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Radiotherapy for gastric mucosa-associated lymphoid tissue lymphoma

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World Journal of Gastrointestinal Oncology 2021年第10期13卷 1453-1465页

作者： Laurent Quéro Mouna Labidi Marc Bollet Côme Bommier Sophie Guillerm Christophe Hennequin Catherine Thieblemont Department of Radiation Oncology AP-HPSaint Louis HospitalParis 75010France Faculty of Medicine Universitéde ParisParis 75005France Department of Radiation Oncology Hartmann Oncology Radiotherapy GroupLevallois-Perret 92044France Hemato-Oncology DMU DHIAP-HPSaint Louis HospitalParis 75010France

Gastric mucosa-associated lymphoid tissue(MALT)lymphoma is a rare disease which is often associated with Helicobacter pylori(***)***-line treatment of stage IE and IIE localized gastric MALT lymphoma is based on the eradication of *** presence of *** resistance factors such as translocation t(11;18),peri-gastric lymph node involvement and the degree of tumor infiltration of the gastric wall;or lack of response to antibiotic therapy are two main indications to treat with definitive radiotherapy(RT).RT is an effective treatment in localized gastric MALT lymphoma.A moderate dose of 30 Gy allows a high cure rate while being well *** treatment,regular gastric endoscopic follow-up is necessary to detect a potential occurrence of gastric adenocarcinoma.

关键词： Radiotherapy Mucosa-associated lymphoid tissue MALT Helicobacter pylori Lymphoma Review

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Anaphylactic shock induced by polyethylene glycol after bowel preparation for the colorectal cancer surgery:A case report

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世界临床病例杂志 2023年第23期11卷 5589-5594页

作者： Go Woon Park Nahyeon Park Jung Cheol Kuk Eung Jin Shin Dae ro Lim Department of General Surgery Soonchunhyang Bucheon HospitalBucheon 14584South Korea

BACKGroUND Polyethylene glycol(PEG)is widely used as an additive because of its hydrophilic and chemically inert ***,there are been increasing reports of PEG allergies,including anaphylaxis,although they are still *** case report aims to raise awareness,that the commonly used bowel cleansing agent containing PEG can cause serious allergic *** SUMMARY Prior to surgery for sigmoid colon cancer,a 63-year-old man was prescribed a bowel cleansing agent containing *** 30 min of ingestion,he developed symptoms of anaphylactic shock and did not respond to initial intramuscular epinephrine *** diagnosis of anaphylaxis to PEG,he was stabilized with fluid hydration and continuous norepinephrine *** While allergic reactions to PEG are rare,they can be ***,it is crucial for clinicians to be aware of this possibility and to diagnose and resuscitate patients immediately.

关键词： Polyethylene glycol Anaphylaxis Sigmoid colon cancer Bowel preparation Case report

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Low-temperature replacement construction of three-dimensional corrosion-resistant interface for deeply rechargeable Zn metal batteries

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纳米材料科学 2024年第3期 329页

作者： Jinze Li Daniel ro hrens Gianluca Dalfollo Xiaochao Wu Ziheng Lu Qiang Gao Bo Han Ruimin Sun Chenggang Zhou Jindi Wang Zhao Cai

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Zeolitic imidazolate framework-67 derived Al-Co-S hierarchical sheets bridged by nitrogen-doped graphene:Incorporation of PANI derived carbon nanorods for solid-state asymmetric supercapacitors

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Journal of Energy Chemistry 2022年第11期31卷 429-445,I0012页

作者： Emad S.Goda Bidhan Pandit Sang Eun Hong Bal Sydulu Singu Seong K.Kim Essam B.Moustafa Kuk ro Yoon Kuk ro Yoon Organic Nanomaterials Lab Department of ChemistryHannam UniversityDaejeon 34054Republic of Korea Gas Analysis and Fire Safety Laboratory Chemistry DivisionNational Institute for Standards136Giza 12211Egypt Department of Materials Science and Engineering and Chemical Engineering Universidad Carlos Ⅲ de MadridAvenida de La Universidad 3028911 LeganésMadridSpain Department of Chemical and Biomolecular Engineering Yonsei UniversitySeoul 03722Republic of Korea Department of Chemical Engineering Hannam University1646 Yuseongdae-roYuseong-guDaejeon 34054Republic of Korea Mechanical Engineering Department Faculty of EngineeringKing Abdulaziz UniversityP.O.Box 80204Jeddah 22254Saudi Arabia

Metal sulfides have been widely enticed as battery-type electrodes in supercapacitor devices because of their maximal theoretical ***,their lower conductivity and ion transport kinetics can largely restrict their rate performance,hence the practical usage in fields of demanding high power ***,the design of new electrodes with higher energy and power densities remains a highly challenging *** the best of our knowledge,a novel hierarchical composite of Al-CoS_(2) on nitrogendoped graphene(NG)is prepared based on a zeolite imidazole framework using a simple and scalable hydrothermal *** this hybrid,ultrathin Al-CoS_(2) nanosheet arrays are vertically orientated on the NG framework to limit self-aggregation,hence increasing the electrical property and cycle stability of *** is investigated that the Al/Co feeding ratio plays a crucial role in controlling the obtained hierarchical structure of Al-Co-S sheets and their electrode ***,Al^(3+) can influence remarkably the morphology and electrochemical property of the resultant graphene *** effective synergism is noticed between the redox Al-CoS_(2) and NG resulting in fast electron transfer and chargingdischarging ***,when the as-developed composite is utilized as a positive electrode at an applied current density of 1 A/g,a specific capacitance of 1915.8 F/g is attained with ultra-long cycle stability(96%,10,000 cycles)and an excellent retention rate(~89%).As a consequence,when a solid-state asymmetric supercapacitor(ASC)device is made by combining an Al-CoS_(2) @NG hybrid with a negative electrode made of polyaniline(PANI)derived carbon nanorods(PCNRs),it demonstrates remarkable specific capacitance(188 F/g),energy density(66.9 Wh/kg),and cyclic stability of 92%after 10,000 *** may open the pathway for the application of the next-generation supercapacitors in the future.

关键词： Hierarchical sheets MOFs Al-doped CoS_(2) Nitrogen-doped graphene Solid-state supercapacitor

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Maxwellian Averaged Neutron Capture Cross Sections in 56Fe and 57Fe

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Chinese Physics Letters 2014年第9期31卷 29-31页

作者：王涛峰李万雨金贵年 ro Tae-Ik KANG Yeong-rok IGASHIRA Masayuki KATABUCHI Tatsuya International Research Center for Nuclei and Particles in the Cosmos Beihang University Beijing 100191 School of Physics and Nuclear Energy Engineering Beihang University Beijing 100191 Department of Physics Kyungpook National University Daegu 702-70L Korea Department of Physics Dong-A University Busan 604-714 Korea Research Center Dong-nam Institute of Radiological and Medical Sciences Busan 619-953 Korea Research Laboratory for Nuclear Reactors Tokyo Institute of Technology Tokyo 152-8550 Japan

Measurements of the keV-neutron capture cross sections and radiative γ-ray spectrum of 56Fe and 57Fe are performed based on a 7 Li（p,n）7 Be reaction neutron source. The incident neutron spectrum on a capture sample is measured by means of a time-of-flight （TOF） method with a 6Li-glass detector. The radiative capture 7-rays emitted from an iron CS Fe or 57 Fe） or standard gold （197Au） sample are detected by a large anti-Compton NaI（TI） spectrometer covered with a heavy shield. The capture yields of samples are obtained by applying a pulse-height weighting technique to the corresponding capture γ-ray pulse-height spectrum. The Maxwellian averaged neutron capture cross sections of 56Fe and 5T Fe are derived according to the present capture cross section results.

关键词： NEUTroN capture RESEARCH NEUTroN sources RADIATIVE capture TIME-of-flight measurements NEUTroN capture gamma ray spectroscopy SPECTRA

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Effect of electrical stimulation on neural regeneration via the p38-RhoA and ERK1/2-Bcl-2 pathways in spinal cord-injured rats

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Neural Regeneration Research 2018年第2期13卷 340-346页

作者： Min Cheol Joo Chul Hwan Jang Jong Tae Park Seung Won Choi Seungil ro Min Seob Kim Moon Young Lee Department of Rehabilitation Medicine and Institute of Wonkwang Medical Science School of Medicine Wonkwang University Department of Neurosurgery School of Medicine Wonkwang University Department of Anesthesiology and Pain Medicine School of Medicine Wonkwang University Department of Physiology and Cell Biology School of Medicine University of Nevada Department of Physiology and Institute of Wonkwang Medical Science School of Medicine Wonkwang University

Although electrical stimulation is therapeutically applied for neural regeneration in patients, it remains unclear how electrical stimulation exerts its effects at the molecular level on spinal cord injury （SCI）. To identify the signaling pathway involved in electrical stimulation improving the function of injured spinal cord, 21 female Sprague-Dawley rats were randomly assigned to three groups： control （no surgical intervention, n = 6）, SCI （SCI only, n = 5）, and electrical simulation （ES; SCI induction followed by ES treatment, n = 10）. A complete spinal cord transection was performed at the 10^th thoracic level. Electrical stimulation of the injured spinal cord region was applied for 4 hours per day for 7 days. On days 2 and 7 post SCI, the Touch-Test Sensory Evaluators and the Basso-Beattie-Bresnahan locomotor scale were used to evaluate rat sensory and motor function. Somatosensory-evoked potentials of the tibial nerve of a hind paw of the rat were measured to evaluate the electrophysiological function of injured spinal cord. Western blot analysis was performed to measure p38-RhoA and ERK1/2-Bcl-2 pathways related protein levels in the injured spinal cord. Rat sensory and motor functions were similar between SCI and ES groups. Com- pared with the SCI group, in the ES group, the latencies of the somatosensory-evoked potential of the tibial nerve of rats were significantly shortened, the amplitudes were significantly increased, RhoA protein level was significantly decreased, protein gene product 9.5 expression, ERK1/2, p38, and Bcl-2 protein levels in the spinal cord were significantly increased. These data suggest that ES can promote the recovery of electrophysiological function of the injured spinal cord through regulating p38-RhoA and ERK1/2-Bcl-2 pathway-related protein levels in the injured spinal cord.

关键词： Bcl-2 ERKI/2 p38 PGP9.5 RhoA spinal cord injury somatosensory evoked potential muscle contraction electrical impulses neural regeneration

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Sodium alendronate loaded poly(L-lactideco-glycolide)microparticles immobilized on ceramic scaffolds for local treatment of bone defects

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Regenerative Biomaterials 2020年第3期7卷 293-302页

作者： Lucja Rumian Cornelia Wolf-Brandstetter Sina roβler Katarzyna Reczynska Hanna Tiainen Havard J.Haugen Dieter Scharnweber Elzbieta Pamula Faculty of Materials Science and Ceramics Department of Biomaterials and CompositesAGH University of Science and TechnologyAl.A.Mickiewicza 30Krakow 30-059Poland Technische Universitat Dresden Institute of Materials ScienceMax Bergmann Center of BiomaterialsBudapester Str.27Dresden 01-069Germany Department of Biomaterials Institute for Clinical DentistryUniversity of OsloGeitmyrsveien 71BlindernP.O.Box 1109Oslo NO-0317Norway

Bone tissue regeneration in critical-size defects is possible after implantation of a 3D scaffold and can be additionally enhanced once the scaffold is enriched with drugs or other factors supporting bone remodelling and *** alendronate(Aln),a widely used anti-osteoporosis drug,exhibits strong inhibitory effect on bone resorption performed by ***,we propose a new approach for the treatment of bone defects in craniofacial region combining biocompatible titanium dioxide scaffolds and poly(L-lactide-co-glycolide)microparticles(MPs)loaded with *** MPs were effectively attached to the surface of the scaffolds’pore walls by human recombinant *** release from the scaffolds was characterized by initial burst(2466% of the drug released within first 24 h)followed by a sustained release phase(on average 5 mg of Aln released per day from Day 3 to Day 18).In vitro tests evidenced that Aln at concentrations of 5 and 2.5 mg/ml was not cytotoxic for MG-63 osteoblast-like cells(viability between 8166% and 9863% of control),but it prevented RANKL-induced formation of osteoclast-like cells from macrophages derived from peripheral blood mononuclear cells,as shown by reduced fusion capability and decreased tartrateresistant acid phosphatase 5b activity(5665% reduction in comparison to control after 8 days of culture).Results show that it is feasible to design the scaffolds providing required doses of Aln inhibiting osteoclastogenesis,reducing osteoclast activity,but not affecting osteoblast functions,which may be beneficial in the treatment of critical-size bone tissue defects.

关键词： ceramic scaffolds sodium alendronate osteoblasts osteoclastogenesis collagen critical-size defect poly(L-lactideco-glycolide) microparticles

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