Cutaneous Dengue Virus Inoculation Triggers Strong B Cell Reactions but Contrastingly Poor T Cell Responses

作     者：Edith Marcial-Juárez Julio García-Cordero Raúl Antonio Maqueda-Alfaro Rafael Eduardo Saucedo-López Luvia Enid Sánchez-Torres Leticia Cedillo-Barrón Leopoldo Flores-Romo Edith Marcial-Juárez;Julio García-Cordero;Raúl Antonio Maqueda-Alfaro;Rafael Eduardo Saucedo-López;Luvia Enid Sánchez-Torres;Leticia Cedillo-Barrón;Leopoldo Flores-Romo

作者机构：Department of Cell BiologyCenter for Advanced Research(CINVESTAV-IPN)The National Polytechnic Institute07360 Mexico CityMexico Department of Molecular BiomedicineCenter for Advanced Research(CINVESTAV-IPN)The National Polytechnic Institute07360 Mexico CityMexico Immunology DepartmentThe National School of Biological Sciences(ENCB-IPN)The National Polytechnic Institute11340 Mexico CityMexico 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2020年第35卷第5期

页      面：575-587页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：supported by a grant from CONACYT-Mexico(221102)to Leopoldo Flores-Romo by a CINVESTAV grant to Leticia Cedillo-Barrón 

主　　题：Dengue virus(DENV) B and T cells proliferation Lymphocyte activation Lymph nodes In vivo cutaneous infection Immunocompetent mice 

摘      要：Dengue is a global health problem without current specific treatment nor safe vaccines *** severe dengue is related to pre-existing non-neutralizing dengue virus(DENV)antibodies,the role of T cells in protection or pathology is *** cutaneous DENV infection in immunocompetent mice we previously showed the generation of PNA+germinal centers(GCs),now we assessed the activation and proliferation of B and T cells in draining lymph nodes(DLNs).We found a drastic remodelling of DLN compartments from 7 to 14 days post-infection(dpi)with greatly enlarged B cell follicles,occupying almost half of the DLN area compared to*24%in na?ve *** clusters of proliferating(Ki-67+)cells inside B follicles were found 14 dpi,representing*33%of B cells in DLNs but only*2%in noninfected *** GCs,we noticed an important recruitment of tingle body macrophages removing apoptotic *** contrast,the percentage of paracortex area and total T cells decreased by 14–16 dpi,compared to *** randomly distributed Ki-67+T cells were found,similar to non-infected ***69 expression by CD4+and CD8+T cells was minor,while it was remarkable in B cells,representing 1764.7%of change from basal levels 3 *** apparent lack of T cell responses cannot be attributed to apoptosis since no significant differences were observed compared to noninfected *** study shows massive B cell activation and proliferation in DLNs upon DENV *** contrast,we found very poor,almost absent CD4+and CD8+T cell responses.