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Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence

Light(Science & Applications) 2023年第3期12卷 415-424页

作者： Leanne Maurice alberto bilenca Biomedical Engineering Department Ben-Gurion University of the Negev1 Ben Gurion BlvdBe’er-Sheva84105Israel Ilse Katz Institute for Nanoscale Science and Technology Ben-Gurion University of the Negev1 Ben Gurion BlvdBe’er-Sheva84105Israel

Single particle tracking in three dimensions is an indispensable tool for studying dynamic processes in various disciplines,including material sciences,physics,and biology,but often shows anisotropic three-dimensional spatial localization precision,which restricts the tracking precision,and/or a limited number of particles that can be tracked simultaneously over extended *** we developed an interferometric,three-dimensional fluorescence single particle tracking method based on conventional widefield excitation and temporal phase-shift interference of the emitted,high-aperture-angle,fluorescence wavefronts in a greatly simplified,free-running,triangle interferometer that enables tracking of multiple particles at the same time with<10-nm spatial localization precision in all three dimensions over extended volumes(~35×35×2μm^(3))at video rate(25 Hz).We applied our method to characterize the microenvironment of living cells and up to~40μm deep in soft materials.

关键词： materials particle dimensions

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The seasonal role of field characteristics on seed-eating bird abundances in agricultural landscapes

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Current Zoology 2017年第3期63卷 279-286页

作者： Emmanuel ZUFIAURRE Mariano CODESIDO Agustin M. ABBA David bilenca Grupo de Estudios sobre Biodiversidad en Agroecosistemas (6EBA) Departamento de Biodiversidad y Biologia Experimental Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires and IEGEBA (UBA- CONICET). Ciudad Universitaria Pabellon II 4° Piso (C1428EHA). Ciudad Autonoma de Buenos Aires Argentina Centro de Estudios Parasitol6gicos y de Vectores (CEPAVE) CCT-CONICET-LA PLATA-UNLP. Calle 120 entre 61 y 62 S/N La Plata (B1902CHX) Buenos Aires Argentina

In temperate agroecosystems, avian responses in abundance and distribution to landscape attributes may be exacerbated by the coupling of natural seasons and farming practices. We assessed the sea- sonal roles of field type, field use in the surroundings, and distance from a field to the nearest woodlot on the abundance of seed-eating birds in a 225,000 km2 study area in the Pampas of central Argentina. During spring-summer and autumn of 2011-2013, we randomly selected 392 fields and used transect samples to collect data on abundance and presence of seed-eating bird species. We recorded a total of 11,579 individuals belonging to 15 seed-eating bird species. We used generalized lineal mixed models to relate bird abundance to field type, field use in the surroundings, and distance to the nearest woodlot. In spring-summer （breeding season） most bird responses were associated with their nesting requirements. Species that build their nests in trees, such as eared doves Zenaida auriculata, picazuro pigeons Patagioenas picazuro, and monk parakeets Myiopsitta monachus, were more abundant in fields closer to woodlots, whereas grassland yellow-finches Sicalis luteola, which nest at areas with tall grasses, were more abundant in fields with livestock use patches in the field surroundings. In au- tumn （non-breeding season）, most bird responses were associated with foraging and refuge needs. The high abundance of eared doves in crop stubbles and the association of pigeons at field surroundings dominated by croplands or at crop stubbles surrounded by livestock use fields revealed the intimate association of these species to sites with high availability of food resources. In addition, both picazuro pigeons and spot-winged pigeons Patagioenas maculosa were associated with woodlots, which provide suitable roosting sites. Our results show that in temperate agroecosystems, the relationships between field characteristics and seed-eating bird abundances vary with season.

关键词： field surroundings field type granivorous birds stubbles temperate agroecosystems woodlots.

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